Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver.

abstract：:The fluorescence characteristics of product (I), formed during the lipid peroxidation of rat liver phosphatidylcholine liposomes containing glycine, and fluorescent product (II), derived from the reaction of malonaldehyde with glycine, were examined to elucidate the mechanism of fluorescent chromophore formation. Fluo...

journal_title：Journal of biochemistry

authors： Shimasaki H,Hirai N,Ueta N

更新日期：1988-11-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00

abstract：:Translesion DNA synthesis (TLS) involves PCNA mono-ubiquitination and TLS DNA polymerases (pols). Recent evidence has shown that the mono-ubiquitination is induced not only by DNA damage but also by other factors that induce stalling of the DNA replication fork. We studied the effect of spontaneous DNA replication err...

journal_title：Journal of biochemistry

authors： Suzuki M,Niimi A,Limsirichaikul S,Tomida S,Miao Huang Q,Izuta S,Usukura J,Itoh Y,Hishida T,Akashi T,Nakagawa Y,Kikuchi A,Pavlov Y,Murate T,Takahashi T

更新日期：2009-07-01 00:00:00

abstract：:Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet react...

journal_title：Journal of biochemistry

authors： Takahashi C,Nakakita S,Hase S

更新日期：2003-07-01 00:00:00

abstract：:We prepared human rheumatoid arthritic synovial fluid phospholipase A2 (PLA2) [EC 3.1.1.4] from insect cells infected with a recombinant baculovirus. The PLA2 DNA was designed, changing the original codons to those used frequently in the polyhedrin gene. Sixteen oligo-deoxynucleotides ranging from 40 to 70 nucleotides...

journal_title：Journal of biochemistry

authors： Kawauchi Y,Takasaki J,Matsuura Y,Masuho Y

更新日期：1994-07-01 00:00:00

abstract：:Human neutrophil elastase was purified to homogeneity as two isozymes named E1 and E2. The isozymes degraded Type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan similarly to each other. The degradation of such basement membrane components by elastase may assist the extravasation of neutrophils in ...

journal_title：Journal of biochemistry

authors： Watanabe H,Hattori S,Katsuda S,Nakanishi I,Nagai Y

更新日期：1990-11-01 00:00:00

abstract：:To improve our understanding of the physiological roles of parvalbumins, PA-1 (pI 4.78) and PA-2 (pI 4.97) parvalbumins were prepared from bullfrog skeletal muscle and their calcium binding properties were examined in a medium of constant ionic strength (I = 0.106, pH 6.80, at 20 degrees C) containing various concentr...

journal_title：Journal of biochemistry

authors： Ogawa Y,Tanokura M

更新日期：1986-01-01 00:00:00

abstract：:Kinetic analyses of the hydrolysis reactions of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside [(GlcNAc)(2)-UMB (1), GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)(2)-UMB (4)] by ChiA and ChiB from Serratia marcescens were performed. Both enzymes released UMB from all compounds ...

journal_title：Journal of biochemistry

authors： Honda Y,Kitaoka M,Tokuyasu K,Sasaki C,Fukamizo T,Hayashi K

更新日期：2003-02-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00

abstract：:The alpha-subunit of rod photoreceptor G protein transducin (G(t1)alpha) is heterogeneously modified at the N-terminus by a mixture of acyl groups, laurate (C12:0), myristate (C14:0), and two unsaturated fatty acids (C14:1 and C14:2). Although the N-fatty acylation of G(t1)alpha plays important roles in protein-protei...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Matsuda T,Matsuura Y,Haga T,Fukada Y

更新日期：2004-03-01 00:00:00

abstract：:Rat liver glucocorticoid receptor was partially purified and characterized for its hormone binding using selenite. Selenite at very low concentrations irreversibly inhibited the hormone binding. The concentration for half maximal inhibition was approximately 2.8 microM. The inhibition was restored by dithiothreitol. T...

journal_title：Journal of biochemistry

authors： Tashima Y,Terui M,Itoh H,Mizunuma H,Kobayashi R,Marumo F

更新日期：1989-03-01 00:00:00

abstract：:1. The relation between intrinsic viscosity in 0.2 M NaCl and molecular weight for samples of chondroitin sulfate isolated from shark cartilage could be expressed by [eeta] = 6.5 X 10(-6) Mr114. 2. Their sedimentation coefficients were linear with respect to the square root of molecular weight, and the intercept was n...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-03-01 00:00:00

abstract：:To investigate the effect of plasminogen on cartilage catabolism, we assessed collagen degradation in rabbit articular cartilage explants treated with or without plasminogen and interleukin-1alpha (IL-1alpha). The combination of IL-1 alpha and plasminogen induced rapid collagen degradation, amounting to more than 60% ...

journal_title：Journal of biochemistry

authors： Saito S,Katoh M,Masumoto M,Matsumoto S,Masuho Y

更新日期：1997-07-01 00:00:00

abstract：:The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extrac...

journal_title：Journal of biochemistry

authors： Igawa T,Higashi S,Abe Y,Ohkuri T,Tanaka H,Morimoto S,Yamashita T,Tsuda M,Inoue K,Ueda T

更新日期：2013-03-01 00:00:00

abstract：:Hepatic peroxisomes and mitochondria from 20-day-old chick embryo were separated by sucrose density gradient centrifugation and the characteristics of carnitine acyltransferases in these organelles were studied. The carnitine acyltransferase activities in peroxisomes were increased markedly by the treatment of chick e...

journal_title：Journal of biochemistry

authors： Ishii S,Ishii H,Suga T

更新日期：1985-09-01 00:00:00

abstract：:p-Nitrophenyl O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha- D-glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-alpha-D-glucopyranoside (FG5P) is hydrolyzed by human pancreatic a-amylase (HPA) or salivary alpha-amylase (HSA) to O-6-deoxy-6-[(2-pyridy...

journal_title：Journal of biochemistry

authors： Omichi K,Ikenaka T

更新日期：1986-11-01 00:00:00

abstract：:Micromolar levels of free calcium ions added to the extracellular medium elicit secretion of serotonin from electropermeabilized bovine platelets in the presence of millimolar levels of Mg-ATP. Such Ca2(+)-dependent secretion of serotonin was almost completely impaired when the permeabilized platelets were preincubate...

journal_title：Journal of biochemistry

authors： Morimoto T,Oho C,Ueda M,Ogihara S,Takisawa H

更新日期：1990-08-01 00:00:00

abstract：:Induction of the expression of the beta-lactamase gene, blaB, is regulated by the blaA gene located just upstream of blaB in the opposite direction in Proteus vulgaris. The expression of the blaA gene is negatively autoregulated by its own product BlaA, the activator of the blaB gene. The P. vulgaris BlaA protein shar...

journal_title：Journal of biochemistry

authors： Ishiguro K,Sugimoto K

更新日期：1996-07-01 00:00:00

abstract：:Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

journal_title：Journal of biochemistry

authors： Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

更新日期：1989-11-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...

journal_title：Journal of biochemistry

authors： Kabashima T,Kitazono A,Kitano A,Ito K,Yoshimoto T

更新日期：1997-09-01 00:00:00

abstract：:"DNase binding protein" of 43K daltons as determined by SDS-polyacrylamide gel electrophoresis, was purified from the 0.1 M KCl-soluble (non-structural) fraction of chicken skeletal muscle. The protein was distinct from actin in amino acid composition and physicochemical properties. "DNase binding protein" was also is...

journal_title：Journal of biochemistry

authors： Kohama K,Holtzer H

更新日期：1981-02-01 00:00:00

abstract：:Previously, we isolated t-cinnamoyl-D-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. The enzyme which catalyzes the formation of t-cinnamoyl-D-glucose has been purified 539-fold from sweet potato root (Ipomoea batatas Lam.) and characterized. It required UDP-glucose as a gl...

journal_title：Journal of biochemistry

authors： Shimizu T,Kojima M

更新日期：1984-01-01 00:00:00

abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

journal_title：Journal of biochemistry

authors： Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

更新日期：2002-02-01 00:00:00

abstract：:The heavy chain of myosin subfragment-1 prepared by chymotrypsin treatment had a molecular weight of about 96 K. It was split into 26 K, 50K, and 21 K fragments on trypsin treatment. The effect of actin binding on the susceptibilities of the junctions between 26 K and 50 K and between 50 K and 21 K, and on that of alk...

journal_title：Journal of biochemistry

authors： Yamamoto K,Sekine T

更新日期：1979-12-01 00:00:00

abstract：:Bispecific antibody targeting of two different antigens is promising, but when fragment-based antibodies are used, homogeneous production is difficult. To overcome this difficulty, we developed a method using the SpyTag/SpyCatcher system in which a covalent bond is formed between the two polypeptides. Using this metho...

journal_title：Journal of biochemistry

authors： Yumura K,Akiba H,Nagatoishi S,Kusano-Arai O,Iwanari H,Hamakubo T,Tsumoto K

更新日期：2017-09-01 00:00:00

abstract：:Rhizopus delemar (ATCC 34612) lipase was modified by phospholipid (PL)-treatment so as to enhance its activity on lipoprotein. In order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove PL from the PL-treated lipase solution which included PL nonessential t...

journal_title：Journal of biochemistry

authors： Iwai M,Shimada Y,Tsujisaka Y

更新日期：1980-08-01 00:00:00

abstract：:C3-translational product, which was synthesized with rabbit liver mRNA in a reticulocyte lysate protein-synthesizing system, did not react with [14C]methylamine, indicating the lack of an internal thioester bond. Instead, the C3-translational product reacted with iodo[1-14C]acetamide, as determined by gel electrophore...

journal_title：Journal of biochemistry

authors： Iijima M,Tobe T,Sakamoto T,Tomita M

更新日期：1984-11-01 00:00:00

abstract：:In order to stabilize human RNase 1 by introduction of an intramolecular cross-link, a mutant protein (4-118CL RNase 1), in which Arg4 and Val118 are replaced with cysteine residues and linked by a disulfide bond, was designed and expressed in Escherichia coli as inclusion bodies. The 4-118CL RNase 1 that refolded und...

journal_title：Journal of biochemistry

authors： Futami J,Tada H,Seno M,Ishikami S,Yamada H

更新日期：2000-08-01 00:00:00

abstract：:Studies with crude preparations obtained from a cysteine auxotroph of Saccharomyces cerevisiae showed that O-acetylserine sulfhydrylase could be separated from O-acetylhomoserine sulfhydrylase by chromatography on a DEAE-cellulose column and centrifugation in a sucrose density gradient. On the basis of sedimentation d...

journal_title：Journal of biochemistry

authors： Yamagata S

更新日期：1980-11-01 00:00:00