Abstract:
:The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extracellular domain of the rat P2X4 receptor expressed in Escherichia coli. Then, we established a B-cell hybridoma producing the monoclonal antibody for the head domain of the rat P2X4 receptor with strict recognition, including S-S bond formation. In addition, we succeeded in the detection and immune precipitation of rat P2X4 receptor molecules on cultured cells. As the antibody specifically binds to the rat P2X4 receptor molecule, it is expected that the established monoclonal antibody will be applicable as a tool for detecting increasing expression levels of the P2X4 receptor molecule accompanied with increasing intensity of neuropathic pain.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Igawa T,Higashi S,Abe Y,Ohkuri T,Tanaka H,Morimoto S,Yamashita T,Tsuda M,Inoue K,Ueda Tdoi
10.1093/jb/mvs143subject
Has Abstractpub_date
2013-03-01 00:00:00pages
275-82issue
3eissn
0021-924Xissn
1756-2651pii
mvs143journal_volume
153pub_type
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133000
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pub_type: 杂志文章
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