Introduction of a series of alkyl thiomaltosides, useful new non-ionic detergents, to membrane biochemistry.

abstract：:Interleukin-1 (IL-1) is produced and released by various cells, including activated macrophages, and plays important roles in inflammation as well as immune responses. Since the precursor of IL-1 has no signal peptide, the mechanism of IL-1 release has been an enigma. To investigate the possibility of direct interacti...

journal_title：Journal of biochemistry

authors： Oku N,Saito N,Okada S,Watanabe N,Kobayashi Y

更新日期：1995-10-01 00:00:00

abstract：:Genomic DNA libraries were screened for the human histidine-rich glycoprotein (HRG) gene and a sequence of 15,499 nucleotides was determined. The gene is composed of 7 exons and 6 introns, and all the exon-intron boundaries match the consensus GT/AG sequence for donor and acceptor splice sites. Each of cystatin-like d...

journal_title：Journal of biochemistry

authors： Wakabayashi S,Takahashi K,Koide T

更新日期：1999-03-01 00:00:00

abstract：:Rat and mouse cDNAs for Zn-alpha 2-glycoprotein (Zn alpha 2gp) were isolated from liver libraries (lambda gt11) and compared with the human one. The lengths of cDNA inserts analyzed were 1,233 and 1,273 nucleotides for rat and mouse, respectively. The deduced amino acid sequences suggested that rat and mouse Zn alpha ...

journal_title：Journal of biochemistry

authors： Ueyama H,Naitoh H,Ohkubo I

更新日期：1994-09-01 00:00:00

abstract：:Chemical modification of gamma-carboxyglutamic acid (Gla) residues in human prothrombin to gamma-methyleneglutamic acid (gamma-MGlu) residues elicited a conformation similar, if not identical, to that of des-gamma-carboxy prothrombin or PIVKA-II, i.e., prothrombin molecules induced by vitamin K antagonists or vitamin ...

journal_title：Journal of biochemistry

authors： Sugo T,Watanabe K,Naraki T,Matsuda M

更新日期：1990-09-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00

abstract：:We isolated three genomic clones of rat P-450(11 beta) genes (CYP11B). Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively. The third one was a novel gene resembling both CYP11B1 and CYP11B2, and was named CYP11B3 gene (CYP11B3). CYP11B2 and CYP11B3 are ...

journal_title：Journal of biochemistry

authors： Nomura M,Morohashi K,Kirita S,Nonaka Y,Okamoto M,Nawata H,Omura T

更新日期：1993-02-01 00:00:00

abstract：:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...

journal_title：Journal of biochemistry

authors： Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IM

更新日期：1990-05-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:Escherichia coli RNase HII is composed of 198 amino acid residues. The enzyme has been overproduced in an insoluble form, purified in a urea-denatured form, and refolded with poor yield [M. Itaya (1990) Proc. Natl. Acad. Sci. USA 87, 8587-8591]. To facilitate the preparation of the enzyme in an amount sufficient for p...

journal_title：Journal of biochemistry

authors： Ohtani N,Haruki M,Muroya A,Morikawa M,Kanaya S

更新日期：2000-05-01 00:00:00

abstract：:The plant NADPH oxidases, known as respiratory burst oxidase homologues (Rbohs), play an indispensable role in a wide array of cellular and developmental processes. Arabidopsis thaliana RbohF (AtRbohF)-mediated production of reactive oxygen species (ROS) is involved in biotic and abiotic stress responses. Because of t...

journal_title：Journal of biochemistry

authors： Kimura S,Kawarazaki T,Nibori H,Michikawa M,Imai A,Kaya H,Kuchitsu K

更新日期：2013-02-01 00:00:00

abstract：:The nucleotide sequence of 5S rRNA from the fission yeast, S. pombe, has been established by post labeling procedures combined with cataloging RNase T1- and A-oligonucleotides derived from unlabeled 5S rRNA. The sequence consists of 119 nucleotides without a modified base and shows more dissimilarities (at 38 position...

journal_title：Journal of biochemistry

authors： Komiya H,Miyazaki M,Takemura S

更新日期：1981-05-01 00:00:00

abstract：:A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono Q. The enzyme had a...

journal_title：Journal of biochemistry

authors： Ohmoto T,Kinoshita T,Moriyoshi K,Sakai K,Hamada N,Ohe T

更新日期：1998-09-01 00:00:00

abstract：:Partition of merocyanine dyes, which have a negative charge, onto photosynthetic membranes of chloroplasts and bacteria was analyzed by measuring the fluorescence intensity change, absorbance change, and amount of dye in the supernatant after centrifugation. The partition depended on the surface potential, which is a ...

journal_title：Journal of biochemistry

authors： Masamoto K,Matsuura K,Itoh S,Nishimura M

更新日期：1981-02-01 00:00:00

abstract：:The mechanism by which GTP induces Ca2+ release from Ca2(+)-preloaded rat hepatic microsomes was studied. In the same concentration range as that for Ca2+ release, GTP inhibited the initial rate of ATP-driven Ca2+ uptake. It also inhibited the formation by ATP of the phosphorylated intermediate of Ca2(+)-ATPase, which...

journal_title：Journal of biochemistry

authors： Kimura S,Higham S,Robison BC,Kraus-Friedmann N

更新日期：1990-04-01 00:00:00

abstract：:Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Tagaya M,Mizushima S

更新日期：1997-02-01 00:00:00

abstract：:Interaction between protein and phospholipid molecules in sarcoplasmic reticulum (SR) was studied by measurement of the conformational fluctuation of the protein using the kinetics of the hydrogen-deuterium exchange reaction. It was revealed that the state of SR ATPase undergoes a phase transition at about 18 degrees ...

journal_title：Journal of biochemistry

authors： Kirino Y,Anzai K,Shimizu H,Ohta S,Nakanishi M,Tsuboi M

更新日期：1977-10-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00

abstract：:It has been found in the brush border membrane vesicles from porcine small intestine that valinomycin causes vesicle aggregation and that Ca2+-induced aggregation of the vesicles is saturably stimulated in the presence of valinomycin. The apparent half-maximal concentration of valinomycin required to enhance the Ca2+ ...

journal_title：Journal of biochemistry

authors： Ohyashiki T,Kodera M,Mohri T

更新日期：1984-09-01 00:00:00

abstract：:Studies on human cell hybrids of a cervical carcinoma cell line, HeLa, and normal fibroblasts have indicated that the tumorigenicity of these cells is under the control of a putative tumor suppressor on chromosome 11, although the nature of this suppressor remains unknown. We examined the expression of caveolin-1, a p...

journal_title：Journal of biochemistry

authors： Suzuki T,Suzuki Y,Hanada K,Hashimoto A,Redpath JL,Stanbridge EJ,Nishijima M,Kitagawa T

更新日期：1998-08-01 00:00:00

abstract：:1. Fractionation of fibroin prepared from the posterior silk glands of Bombyx mori was carried out. After carboxymethylation of the fibroin, it was fractionated by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-cellulose column chromatography. 2. The fibroin was composed of at least two protein...

journal_title：Journal of biochemistry

authors： Shimura K,Kikuchi A,Ohtomo K,Katagata Y,Hyodo A

更新日期：1976-10-01 00:00:00

abstract：:Myosin subfragment-1.pyrophosphate (S1.PPi) complex and S1.ADP complex were observed with 31P NMR at various temperatures between 0 and 25 degrees C. The signal of S1.PPi complex showed a small temperature dependence, indicating that a single conformation exists for the complex. It also showed that the electron densit...

journal_title：Journal of biochemistry

authors： Tanokura M,Ebashi S

更新日期：1993-01-01 00:00:00

abstract：:1. The actin-activated Mg2+-ATPase activity of gizzard HMM increased in proportion to the square of the extent of LC phosphorylation. This result indicates that the LCs of HMM are randomly phosphorylated, and the phosphorylation of both heads of HMM is required for the activation of HMM Mg2+-ATPase by F-actin. 2. In 7...

journal_title：Journal of biochemistry

authors： Ikebe M,Ogihara S,Tonomura Y

更新日期：1982-05-01 00:00:00

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extrac...

journal_title：Journal of biochemistry

authors： Igawa T,Higashi S,Abe Y,Ohkuri T,Tanaka H,Morimoto S,Yamashita T,Tsuda M,Inoue K,Ueda T

更新日期：2013-03-01 00:00:00

abstract：:FOXP2 is a member of the P subfamily of FOX transcription factors, the DNA-binding domain of which is the winged helix forkhead domain (FHD). In this work we show that the FOXP2 FHD is able to bind to various DNA sequences, including a novel sequence identified in this work, with different affinities and rates as dete...

journal_title：Journal of biochemistry

authors： Webb H,Steeb O,Blane A,Rotherham L,Aron S,Machanick P,Dirr H,Fanucchi S

更新日期：2017-07-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:The midpoint pCa value of the fluorescence titration curve of the complex of 2-[4'-iodoacetamido)anilino)-naphthalene-6-sulfonic acid-labeled troponin (IAANS-Tn) and nonpolymerizable tropomyosin (NPTm) was much larger than that for the complex of Tn containing dansylaziridine-labeled troponin C (DANZ-TnC) and NPTm. Th...

journal_title：Journal of biochemistry

authors： Iio T,Nishio T,Kondo H

更新日期：1988-08-01 00:00:00

abstract：:The steric course of the enoyl reduction catalyzed by fatty acid synthetase was investigated with the enzymes from bakers' yeast, rat liver and Brevibacterium ammoniagenes. The non-enzymic hydrogen-deuterium exchange of the methylene group of malonyl-CoA was studied by NMR spectroscopy. The half-life period of the met...

journal_title：Journal of biochemistry

authors： Saito K,Kawaguchi A,Seyama Y,Yamakawa T,Okuda S

更新日期：1981-12-01 00:00:00

abstract：:Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracel...

journal_title：Journal of biochemistry

authors： Kimura T,Yoshida A,Tabuchi Y,Ikari A,Takeguchi N,Asano S

更新日期：2002-06-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00