Permeability change of liposomal membrane induced by interleukin-1 alpha.


:Interleukin-1 (IL-1) is produced and released by various cells, including activated macrophages, and plays important roles in inflammation as well as immune responses. Since the precursor of IL-1 has no signal peptide, the mechanism of IL-1 release has been an enigma. To investigate the possibility of direct interaction of IL-1 with the lipid bilayer, the interleukin-1 alpha (IL-1 alpha)- or beta (IL-1 beta)-induced permeability change of the liposomal membrane was determined. IL-1 alpha, but not IL-1 beta, caused an increase in the permeability of liposomes composed of phosphatidylserine (PS), at neutral and acidic pHs, as demonstrated by measuring the efflux of calcein. On the other hand, liposomes composed of phosphatidylcholine (PC) showed no increase in permeability when incubated with IL-1 alpha, suggesting the importance of acidic phospholipids in the interaction of IL-1 alpha with the membrane. Furthermore, permeability of liposomal membrane was markedly increased by IL-1 alpha in the presence of 1 microM calcium ions, although a permeability change was observed even in the absence of calcium ions. IL-1 alpha also induced the efflux of fluorescent dextran (average M(r) of 39,600), raising the possibility of translocation of IL-1 alpha through the cell membrane.


J Biochem


Journal of biochemistry


Oku N,Saito N,Okada S,Watanabe N,Kobayashi Y




Has Abstract


1995-10-01 00:00:00












  • Inhibition by palmitoyl CoA of dynein ATPase from sea urchin spermatozoa.

    abstract::ATPase of 14S dynein, extracted from spermatozoa of the sea urchin, Hemicentrotus pulcherrimus, and partially purified by sucrose density gradient centrifugation, was inhibited non-competitively by palmitoyl CoA at concentrations higher than 20 microns, and was stimulated at concentrations between 2 microns and 10 mic...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujiwara A,Yokokawa M,Hino A,Yasumasu I

    更新日期:1982-08-01 00:00:00

  • gamma-Carboxyglutamic acid (Gla)-domainless blood coagulation factor IXa species: preparation and properties.

    abstract::To investigate the function of the gamma-carboxyglutamic acid (Gla) residues of factor IXa in the activation of factor X, a new species of bovine factor IXa, designated "factor IXa beta'," and its corresponding Gla-domainless form, designated "Gla-domainless factor IXa beta'," were prepared under controlled conditions...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morita T,Fukudome K,Miyata T,Iwanaga S

    更新日期:1991-12-01 00:00:00

  • Isolation of a stable tetramer of the low molecular weight component of neurofilaments (NF-L).

    abstract::When crude neurofilaments were dissolved in a solution containing 8 M urea and 1% beta-mercaptoethanol (beta-ME), the component proteins of the neurofilaments and other contaminating filaments were solubilized into monomeric forms. However, when reassembled filaments were solubilized again by the addition of urea to 8...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tokutake S,Tanaka K

    更新日期:1989-01-01 00:00:00

  • On the denaturation of porcine erythrocyte catalase with alkali, urea, and guanidine hydrochloride in relation to its subunit structure.

    abstract::The dissociation of porcine erythrocyte catalase [EC] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takeda A,Hirano K,Shiroya Y,Samejima T

    更新日期:1983-04-01 00:00:00

  • Primary structure and autoproteolysis of brevilysin H6 from the venom of Gloydius halys brevicaudus.

    abstract::The complete amino acid sequence of brevilysin H6 (H6), a zinc-protease isolated from Gloydius halys brevicaudus venom, was determined by a manual Edman degradation method. H6 has an amino-terminal pyroglutamic acid and consists of a total of 419 residues. An N-linked sugar chain is attached at Asn-181. The molecule i...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujimura S,Oshikawa K,Terada S,Kimoto E

    更新日期:2000-08-01 00:00:00

  • Synthesis of various phosphodiesters and phosphomonoesters with ribonuclease N.

    abstract::1. 3'-Guanylyl-ethanol, 3'-guanylyl-propanol, and 3'-guanylyl-alpha-glycerol were synthesized by ribonuclease N1 [EC] using guanosine 2',3'-cyclic phosphate as a phosphate donor and various alcohols as phosphate acceptors. The yields of these phosphodiesters were 15%, 13.5%, 38.2%, respectively, with respect t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tamanoi F,Uchida T,Egami F,Oshima T

    更新日期:1976-07-01 00:00:00

  • Aminoacyl transfer RNA formation. VII. Lack of correlation between aminoacylation and PPi-ATP exchange catalyzed by isoleucyl-tRNA synthetase of Escherichia coli in the presence of various divalent cations.

    abstract::Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takeda Y,Ohnishi T,Ogiso Y

    更新日期:1976-09-01 00:00:00

  • The role of nuclear serine proteases in the degradation of newly synthesized histones and ribosomal proteins.

    abstract::To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsurugi K,Oyanagi M,Ogata K

    更新日期:1983-05-01 00:00:00

  • Purification and characterization of 7 beta-hydroxysteroid dehydrogenase from Ruminococcus sp. of human intestine.

    abstract::7 beta-Hydroxysteroid dehydrogenase (7 beta-HSD) was produced by Ruminococcus sp. PO1-3 obtained from among human intestinal bacteria. The enzyme was purified from a crude extract by ammonium sulfate fractionation, and Butyl-Toyopearl 650M, Sephadex G-150, Matrex Red A and Octyl-Sepharose chromatographies. The purifie...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Akao T,Akao T,Kobashi K

    更新日期:1987-09-01 00:00:00

  • Enhancement of transfection efficiency by protamine in DDAB lipid vesicle-mediated gene transfer.

    abstract::We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: You J,Kamihira M,Iijima S

    更新日期:1999-06-01 00:00:00

  • Importance of the ionic nature of ionic liquids in affecting enzyme performance.

    abstract::The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF(6)], [BMIm][BF(4)]), and [BMIm][MeSO(4)], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO(4), KPF(6), and NaBF(4)) on the enzyme performance wa...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yang Z,Yue YJ,Huang WC,Zhuang XM,Chen ZT,Xing M

    更新日期:2009-03-01 00:00:00

  • Cloning and characterization of phosphoglucomutase and phosphomannomutase derived from Sphingomonas chungbukensis DJ77.

    abstract::The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous w...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yoon SS,Park SH,Kim YC,Shin M,Chong CK,Choi JD

    更新日期:2008-10-01 00:00:00

  • Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver.

    abstract::D-Erythrulose reductase of beef liver was crystallized from ammonium sulfate solution at pH 8.17. The crystals are needle-shaped. The enzyme protein contains 851 amino acid residues per mole of the enzyme: Lys28, His11, Arg52, Asp79, Thr58, Ser56, Glu68, Pro20, Gly80, Ala107, Val112, Met24, Ile31, Leu88, Tyr7, Phe22, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Uehara K,Tanimoto T

    更新日期:1975-09-01 00:00:00

  • Intestinal absorption of bile alcohols.

    abstract::The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hoshita T,Harada N,Morita I,Kihira K

    更新日期:1981-11-01 00:00:00

  • Amino acid sequence of the 203-residue fragment of the heavy chain of chicken gizzard myosin containing the SH1-type cysteine residue.

    abstract::A fluorescent fragment of Mr = 23,800 was obtained by the papain digestion of N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene diamine (abbreviated as IAEDANS)-modified chicken gizzard myosin. The fragment was isolated by gel filtration on a Sephadex G-100 column in the presence of 5 M guanidine-HCl followed by anion exch...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Onishi H,Maita T,Miyanishi T,Watanabe S,Matsuda G

    更新日期:1986-12-01 00:00:00

  • Biochemical characterization of endonuclease V from the hyperthermophilic archaeon, Pyrococcus furiosus.

    abstract::Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kiyonari S,Egashira Y,Ishino S,Ishino Y

    更新日期:2014-05-01 00:00:00

  • Tissue-specific regulation of renin-binding protein gene expression in rats.

    abstract::Rat gene for renin-binding protein (RnBP) was shown to be expressed in the kidney, adrenal gland, brain, lung, spleen, ovary, testis, and heart. On sodium depletion and captopril administration, the rat showed a marked increase in the adrenal RnBP mRNA level and a slight decrease in the kidney RnBP mRNA level. In two-...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tada M,Takahashi S,Miyano M,Miyake Y

    更新日期:1992-08-01 00:00:00

  • A spin-label and hydrogen-deuterium exchange reaction kinetics study of protein-lipid interactions in lipid-replaced Ca2+-ATPase of rabbit skeletal muscle sarcoplasmic reticulum.

    abstract::The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Higashi K,Kirino Y

    更新日期:1983-12-01 00:00:00

  • Effect of dietary proteins on the turnover of rat liver argininosuccinate synthetase.

    abstract::The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (K...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuda M,Shikata Y,Katsunuma T

    更新日期:1979-03-01 00:00:00

  • Human neutrophil elastase: degradation of basement membrane components and immunolocalization in the tissue.

    abstract::Human neutrophil elastase was purified to homogeneity as two isozymes named E1 and E2. The isozymes degraded Type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan similarly to each other. The degradation of such basement membrane components by elastase may assist the extravasation of neutrophils in ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Watanabe H,Hattori S,Katsuda S,Nakanishi I,Nagai Y

    更新日期:1990-11-01 00:00:00

  • Characterization of cyanide-resistant respiration and appearance of a 36 kDa protein in mitochondria isolated from antimycin A-treated Hansenula anomala.

    abstract::Mitochondria exhibiting cyanide-resistant respiration were isolated from Hansenula anomala which had been incubated in the presence of antimycin A to induce cyanide-resistant respiration. The cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin A or myxothiazol, suggesting that the bra...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sakajo S,Minagawa N,Komiyama T,Yoshimoto A

    更新日期:1990-08-01 00:00:00

  • Deletion analysis of protein kinase Calpha reveals a novel regulatory segment.

    abstract::Using a combined pharmacological and genetic approach, we have identified aa 260-280 in the C2 region as a critical factor in the catalytic function of protein kinase Calpha (PKCalpha). Progressive truncations from the N-terminus as well as selected internal deletion mutants were expressed in Saccharomyces cerevisiae ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Rotenberg SA,Zhu J,Hansen H,Li XD,Sun XG,Michels CA,Riedel H

    更新日期:1998-10-01 00:00:00

  • A simple and effective method for hemolysis with a hypoxanthine-xanthine oxidase system and alteration of erythrocyte phospholipid composition during the hemolysis.

    abstract::A very rapid hemolysis was found to be caused by active oxygen species produced by a hypoxanthine-xanthine oxidase system with very low concentrations of hypoxanthine. The addition of superoxide dismutase or catalase inhibited the hemolysis, indicating that O2- and H2O2 participate in this system. The extent of erythr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Taniguchi M,Aikawa M,Sakagami T

    更新日期:1981-03-01 00:00:00

  • Process of attachment of phi X174 parental DNA to the host cell membrane.

    abstract::The phi X174-DNA membrane complex was isolated from Escherichia coli infected with phi X174 am3 by isopycnic sucrose gradient centrifugation followed by zone electrophoresis. The phi X174 DNA-membrane complex banded at two positions, intermediate density membrane fraction and cytoplasmic membrane fraction, having bouy...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Azuma J,Morita J,Komano T

    更新日期:1980-08-01 00:00:00

  • Control of Trx1 redox state modulates protection against methyl methanesulfonate-induced DNA damage via stabilization of p21.

    abstract::Thioredoxin 1 (Trx1) is known to play an important role in protecting against cell death. However, the mechanism for control of Trx1 in cell death resulting from DNA damage has not been fully investigated. In this study, we used the DNA-damaging agent methyl methanesulfonate (MMS) to investigate the protective effects...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Gu L,Gao W,Yang HM,Wang BB,Wang XN,Xu J,Zhang H

    更新日期:2016-01-01 00:00:00

  • Characterization of Moloney murine leukaemia virus/avian myeloblastosis virus chimeric reverse transcriptases.

    abstract::Reverse transcriptases (RTs) from Moloney murine leukaemia virus (MMLV) and avian myeloblastosis virus (AMV) contain all the fingers, palm, thumb, connection and RNase H domains. The fingers, palm and thumb domains are thought to be involved in the reverse transcription activity, and the RNase H domain is in the RNase...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yasukawa K,Mizuno M,Inouye K

    更新日期:2009-03-01 00:00:00

  • Proton-relay system of carboxypeptidase Y as a sole catalytic site: studies on mutagenic replacement of his 397.

    abstract::His397 was replaced with alanine by site-directed mutagenesis of the cloned PRC1 gene in order to confirm the role of this residue in the proton-relay system of carboxypeptidase Y (CPY). The expressed and purified H397A showed a CD spectrum almost identical to that of the wild type enzyme, but its heat stability and c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Jung G,Ueno H,Hayashi R

    更新日期:1998-08-01 00:00:00

  • High performance liquid chromatography of the hydroperoxides produced by lipoxygenases.

    abstract::Separation of 13-hydroperoxylinoleic acid or 13-hydroperoxylinolenic acid from linoleic acid or linolenic acid, respectively, was carried out easily and quickly by high performance liquid chromatography on porous polymer gel (TSK-Gel LS-140) using n-hexane/ethanol as an eluent. An eluent containing a large amount of n...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Aoshima H

    更新日期:1977-08-01 00:00:00

  • Roles of nucleoside triphosphates in microtubule assembly.

    abstract::Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kobayashi T,Simizu T

    更新日期:1976-06-01 00:00:00

  • The hydrophobic region of signal peptides is a determinant for SRP recognition and protein translocation across the ER membrane.

    abstract::Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hatsuzawa K,Tagaya M,Mizushima S

    更新日期:1997-02-01 00:00:00