Permeability change of liposomal membrane induced by interleukin-1 alpha.


:Interleukin-1 (IL-1) is produced and released by various cells, including activated macrophages, and plays important roles in inflammation as well as immune responses. Since the precursor of IL-1 has no signal peptide, the mechanism of IL-1 release has been an enigma. To investigate the possibility of direct interaction of IL-1 with the lipid bilayer, the interleukin-1 alpha (IL-1 alpha)- or beta (IL-1 beta)-induced permeability change of the liposomal membrane was determined. IL-1 alpha, but not IL-1 beta, caused an increase in the permeability of liposomes composed of phosphatidylserine (PS), at neutral and acidic pHs, as demonstrated by measuring the efflux of calcein. On the other hand, liposomes composed of phosphatidylcholine (PC) showed no increase in permeability when incubated with IL-1 alpha, suggesting the importance of acidic phospholipids in the interaction of IL-1 alpha with the membrane. Furthermore, permeability of liposomal membrane was markedly increased by IL-1 alpha in the presence of 1 microM calcium ions, although a permeability change was observed even in the absence of calcium ions. IL-1 alpha also induced the efflux of fluorescent dextran (average M(r) of 39,600), raising the possibility of translocation of IL-1 alpha through the cell membrane.


J Biochem


Journal of biochemistry


Oku N,Saito N,Okada S,Watanabe N,Kobayashi Y




Has Abstract


1995-10-01 00:00:00












  • 5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside stimulates the rat enhancer of split- and hairy-related protein-2 gene via atypical protein kinase C lambda.

    abstract::The 5'-AMP-activated protein kinase (AMPK) functions as a cellular energy sensor. 5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside (AICAR) is a chemical activator of AMPK. In the liver, AICAR suppresses expression of thephosphoenolpyruvate carboxykinase(PEPCK) gene. The rat enhancer of split- and hairy-related prot...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Komatsu Y,Yanagisawa Y,Moriizumi M,Tsuchiya Y,Yokouchi H,Otsuka H,Aoyagi M,Tsukada A,Kanai Y,Haneishi A,Takagi K,Asano K,Ono M,Tanaka T,Tomita K,Yamada K

    更新日期:2016-04-01 00:00:00

  • Specific efflux of glutathione from the basolateral membrane domain in polarized MDCK cells during ricin-induced apoptosis.

    abstract::Although the depletion of reduced glutathione (GSH) has been observed in a variety of apoptotic systems, little is known about the mechanism of GSH depletion. In this study we used polarized MDCK cells to study the GSH flux during ricin-induced apoptosis. Here we report that the specific accumulation of GSH occurred i...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda T,Sadakata N,Komatsu N,Muramatsu T

    更新日期:1999-10-01 00:00:00

  • Signalling pathways in the unfolded protein response: development from yeast to mammals.

    abstract::The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Mori K

    更新日期:2009-12-01 00:00:00

  • Partial purification and characterization of UDPG:t-cinnamate glucosyltransferase in the root of sweet potato, Ipomoea batatas Lam.

    abstract::Previously, we isolated t-cinnamoyl-D-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. The enzyme which catalyzes the formation of t-cinnamoyl-D-glucose has been purified 539-fold from sweet potato root (Ipomoea batatas Lam.) and characterized. It required UDP-glucose as a gl...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shimizu T,Kojima M

    更新日期:1984-01-01 00:00:00

  • Metabolism of trichothecene mycotoxins. II. Substrate specificity of microsomal deacetylation of trichothecenes.

    abstract::The substrate specificity of microsomal nonspecific carboxyesterase [EC] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohta M,Matsumoto H,Ishii K,Ueno Y

    更新日期:1978-09-01 00:00:00

  • Purification and characterization of diacylglycerol lipase from human platelets.

    abstract::Diacylglycerol lipase (DGL) was solubilized from human platelet microsomes with heptyl-beta-D-thioglucoside, and purified to homogeneity on SDS-PAGE using a combination of chromatographic and electrophoretic methods. The molecular mass of the purified DGL was estimated to be 33 kDa. Its apparent pI was pH 6.0, as dete...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Moriyama T,Urade R,Kito M

    更新日期:1999-06-01 00:00:00

  • The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils.

    abstract::The degranulation reactions of human neutrophils induced by 1-oleoyl-2-acetylglycerol (OAG), phorbol 12-myristate 13-acetate (PMA), and calcium ionophore A23187 or their combinations, were studied. OAG in the absence of the Ca2+-ionophore A23187 stimulated the releases of both lysozyme and lactoferrin, constituents of...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kang D,Tsuda H,Takeshige K,Shibata Y,Minakami S

    更新日期:1985-12-01 00:00:00

  • Protein N-arginine methylation in subcellular fractions of lymphoblastoid cells.

    abstract::Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Lin CH,Hsieh M,Li YC,Li SY,Pearson DL,Pollard KM,Li C

    更新日期:2000-09-01 00:00:00

  • Active site organization of bacterial type I fatty acid synthetase.

    abstract::Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morishima N,Ikai A

    更新日期:1987-12-01 00:00:00

  • The nudix hydrolase 7 is an Acyl-CoA diphosphatase involved in regulating peroxisomal coenzyme A homeostasis.

    abstract::Coenzyme A (CoASH) is an obligate cofactor for lipids undergoing beta-oxidation in peroxisomes. Although the peroxisomal membrane appears to be impermeable to CoASH, peroxisomes contain their own pool of CoASH. It is believed that CoASH enters peroxisomes as acyl-CoAs, but it is not known how this pool is regulated. T...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Reilly SJ,Tillander V,Ofman R,Alexson SE,Hunt MC

    更新日期:2008-11-01 00:00:00

  • Expression analysis of the aldo-keto reductases involved in the novel biosynthetic pathway of tetrahydrobiopterin in human and mouse tissues.

    abstract::Tetrahydrobiopterin (BH(4)) acts as a cofactor of the aromatic amino-acid hydroxylases, and its deficiency may result in hyperphenylalaninemia (HPA) and decreased production of the neurotransmitters. BH(4) is synthesized by sepiapterin reductase (SPR) from 6-pyruvoyl-tetrahydropterin (PPH(4)). A patient with SPR defic...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hirakawa H,Sawada H,Yamahama Y,Takikawa S,Shintaku H,Hara A,Mase K,Kondo T,Iino T

    更新日期:2009-07-01 00:00:00

  • Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism.

    abstract::In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kagiwada S,Zen R

    更新日期:2003-04-01 00:00:00

  • Potentiometric studies on benzeneboronic acid-alpha-chymotrypsin interactions.

    abstract::When the competitive inhibitor benzeneboronic acid (BBA) forms a complex with alpha-chymotrypsin [EC] protons are released in the acidic pH region. The proton release can be measured by a difference potentiometric technique. The proton release is also observed in chymotrypsinogen A but not in TRCK-, DIP-, and...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hanai K

    更新日期:1976-01-01 00:00:00

  • New gene cluster for lantibiotic streptin possibly involved in streptolysin S formation.

    abstract::Streptolysin S (SLS) is a serum-extractable and oxygen-stable hemolysin produced by Group A Streptococcus. A SLS-deficient mutant in which transposon Tn 916 was inserted in a locus distinct from the sag gene cluster [Nizet et al. (2000) Infect. Immun. 68, 4245-4254] was obtained by filter mating of the transposon-harb...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Karaya K,Shimizu T,Taketo A

    更新日期:2001-05-01 00:00:00

  • Identification of novel splicing variants of protein tyrosine phosphatase receptor type Z.

    abstract::Protein tyrosine phosphatase receptor type Z (PTPRZ, also known as PTPζ or RPTPβ) is preferentially expressed in the central nervous system (CNS). PTPRZ plays important roles during development and adulthood in CNS myelination, learning and memory. Three splicing isoforms for PTPRZ have been identified to date: two re...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujikawa A,Chow JPH,Matsumoto M,Suzuki R,Kuboyama K,Yamamoto N,Noda M

    更新日期:2017-11-01 00:00:00

  • Catalytic properties of NADPH-specific dihydropteridine reductase from bovine liver.

    abstract::The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC]. With quinonoid-dihydro-6-methylpterin, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakanishi N,Hasegawa H,Akino M,Yamada S

    更新日期:1986-03-01 00:00:00

  • Studies on nitrate reductase of Clostridium perfringens. Purification, some properties, and effect of tungstate on its formation.

    abstract::Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Seki-Chiba S,Ishimoto M

    更新日期:1977-12-01 00:00:00

  • A 1H NMR method for the analysis of antigen-antibody interactions: binding of a peptide fragment of lysozyme to anti-lysozyme monoclonal antibody.

    abstract::A proton nuclear magnetic resonance (NMR) study is reported of the molecular structural basis of antigen-antibody interactions. An immunologically reactive proteolytic fragment corresponding to one of the antigenic regions on hen egg-white lysozyme (HEL) was used in combination with a monoclonal antibody that recogniz...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ito W,Nishimura M,Sakato N,Fujio H,Arata Y

    更新日期:1987-09-01 00:00:00

  • Structural characterization of the gene for human histidine-rich glycoprotein, reinvestigation of the 5'-terminal region of cDNA and a search for the liver specific promoter in the gene.

    abstract::Genomic DNA libraries were screened for the human histidine-rich glycoprotein (HRG) gene and a sequence of 15,499 nucleotides was determined. The gene is composed of 7 exons and 6 introns, and all the exon-intron boundaries match the consensus GT/AG sequence for donor and acceptor splice sites. Each of cystatin-like d...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Wakabayashi S,Takahashi K,Koide T

    更新日期:1999-03-01 00:00:00

  • In vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.

    abstract::We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

    更新日期:2020-05-01 00:00:00

  • The structural and functional organization of intramolecular chaperones: the N-terminal propeptides which mediate protein folding.

    abstract::A large number of prokaryotic as well as eukaryotic proteins are produced with amino terminal propeptides. These amino-terminal extensions are essential for mediating proper folding of their corresponding proteins and are also termed as intramolecular chaperones. Though these propeptides are highly specific and unique...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Shinde U,Inouye M

    更新日期:1994-04-01 00:00:00

  • Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein.

    abstract::Hepatitis C virus (HCV) core protein has been suggested to play crucial roles in the pathogeneses of liver steatosis and hepatocellular carcinomas due to HCV infection. Intracellular HCV core protein is localized mainly in lipid droplets, in which the core protein should exert its significant biological/pathological f...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sato S,Fukasawa M,Yamakawa Y,Natsume T,Suzuki T,Shoji I,Aizaki H,Miyamura T,Nishijima M

    更新日期:2006-05-01 00:00:00

  • A novel sulfotransferase abundantly expressed in the dauer larvae of Caenorhabditis elegans.

    abstract::We have isolated a gene (clone Y113G7A.11) from Caenorhabditis elegans (C. elegans), that we have designated as ceST1, and which is the only member of the cytosolic sulfotransferase (SULT) gene family present in the genome of this organism. We identified the SULT motifs of ceST1 based upon their deduced amino acid seq...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hattori K,Inoue M,Inoue T,Arai H,Tamura HO

    更新日期:2006-03-01 00:00:00

  • Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1.

    abstract::The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (Mr 55,830). The predicted amino acid sequence was consistent with the partial a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Xu XM,Koyama N,Cui M,Yamagishi A,Nosoh Y,Oshima T

    更新日期:1991-05-01 00:00:00

  • Characterization and regulation of taurine transport in Caco-2, human intestinal cells.

    abstract::We characterized the taurine transport system in human intestinal Caco-2 cells and showed that it is subject to adaptive regulation. The activity of taurine transport in Caco-2 cells was evaluated by means of an Na+- and Cl(-)-dependent high-affinity transport system, the characteristics of which were similar to those...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Satsu H,Watanabe H,Arai S,Shimizu M

    更新日期:1997-06-01 00:00:00

  • Exogenous type-II phospholipase A2 stimulates prostaglandin synthesis in rat liver-derived BRL-3A cells in the presence of tumor necrosis factor alpha.

    abstract::The effect of extracellular type-II phospholipase A2 (PLA2) on prostaglandin (PG) synthesis has been studied using rat liver-derived BRL-3A cells. The addition of type-II PLA2 to the medium of BRL-3A cells resulted in a marked decrease in the enzymatic activity in the medium. An immunochemical study involving an anti-...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suga H,Murakami M,Kudo I,Inoue K

    更新日期:1995-11-01 00:00:00

  • Importance of Tyr310 residue in the third repeat of microtubule binding domain for filament formation of tau protein.

    abstract::The inhibition of tau fibrillation is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. As a series of studies on inhibiting the transition of soluble monomeric tau into mature fibril, the effect of Tyr310 residue in the third repeat (R3) of the microtubule-binding domain (MBD) on th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishiura C,Takeuchi K,Minoura K,Sumida M,Taniguchi T,Tomoo K,Ishida T

    更新日期:2010-03-01 00:00:00

  • Purification and characterization of 210,000-dalton inhibitor of calcium-activated neutral protease from rabbit skeletal muscle and its relation to 50,000-dalton inhibitor.

    abstract::An endogenous inhibitor of calcium-activated neutral protease (CANP), which was isolated from rabbit skeletal muscle with chemically drastic pretreatments, comprised major (high-molecular-weight form, HMW-inhibitor) and minor (low-molecular-weight form, LMW-inhibitor) components. HMW-inhibitor was purified to homogene...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakamura M,Inomata M,Hayashi M,Imahori K,Kawashima S

    更新日期:1985-09-01 00:00:00

  • Acceleration of the ATPase activity of glycerol-treated muscle fibers by repeated stretch-release cycles.

    abstract::Glycerol-treated muscle fiber bundles were fixed at their rest length in 50 mM KC1, 2 mM MgC1(2), and 10 micron CaC1(2) at pH 7.8 and 0 degrees C in the presence of sufficient amounts of ATP, creatine kinase, and creatine phosphate. The fiber bundles were stretched linearly with time for 0.3 s at a constant amplitude,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Arata T,Mukohata Y,Tonomura Y

    更新日期:1978-10-01 00:00:00

  • Characterization of the human E2F4 promoter region and its response to 12-O-tetradecanoylphorbol-13-acetate.

    abstract::The E2F transcription factors (TFs), which control the progression of the cell cycle in response to DNA-damage and various stresses, are known to interact with a tumor suppressor, Retinoblastoma 1 (RB1). We previously showed that the response of the human RB1 promoter to a 12-O-tetradecanoylphorbol-13-acetate (TPA) in...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hamada H,Goto Y,Arakawa J,Murayama E,Ogawa Y,Konno M,Oyama T,Asai M,Sato A,Tanuma SI,Uchiumi F

    更新日期:2019-06-14 00:00:00