Feedback inhibition by methionine and S-adenosylmethionine, and desensitization of homoserine O-acetyltransferase in Brevibacterium flavum.

abstract：:Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

journal_title：Journal of biochemistry

authors： Sato S,Imamura S,Ozeki Y,Kawaguchi A

更新日期：1992-01-01 00:00:00

abstract：:Rabbit skeletal muscle alpha-tropomyosin (Tm) and the deletion mutant (D234Tm) in which internal actin-binding pseudo-repeats 2, 3, and 4 are missing [Landis et al. (1997) J. Biol. Chem. 272, 14051-14056] were used to investigate the interaction between actin and tropomyosin or actin and troponin (Tn) by means of fluo...

journal_title：Journal of biochemistry

authors： Hai H,Sano K,Maeda K,Maéda Y,Miki M

更新日期：2002-03-01 00:00:00

abstract：:To study the structure/function relationship and enzymatic properties of human aldolase C, we have constructed an Escherichia coli expression plasmid, pHAC11, for the isozyme. E. coli cells carrying this plasmid produced enzymatically active human aldolase C. The kcat and Km values for fructose-1,6-bisphosphate (Fru-1...

journal_title：Journal of biochemistry

authors： Kusakabe T,Motoki K,Hori K

更新日期：1994-06-01 00:00:00

abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...

journal_title：Journal of biochemistry

authors： Hashinotsume M,Higashino K,Hada T,Yamamura Y

更新日期：1978-12-01 00:00:00

abstract：:The three-dimensional structure of rat-liver acyl-CoA oxidase-II (ACO-II) in a complex with a C12-fatty acid was solved by the molecular replacement method based on the uncomplexed ACO-II structure. The crystalline form of the complex was obtained by cocrystallization of ACO-II with dodecanoyl-CoA. The crystalline com...

journal_title：Journal of biochemistry

authors： Tokuoka K,Nakajima Y,Hirotsu K,Miyahara I,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Tojo H,Miura R

更新日期：2006-04-01 00:00:00

abstract：:The planar cell polarity (PCP) protein, Prickle (Pk), is conserved in invertebrates and vertebrates, and regulates cellular morphogenesis and movement. Vertebrate Pk consists of at least two family members, Pk1 and Pk2, both of which are expressed in the brain; however, their localization and function at synapses rema...

journal_title：Journal of biochemistry

authors： Hida Y,Fukaya M,Hagiwara A,Deguchi-Tawarada M,Yoshioka T,Kitajima I,Inoue E,Watanabe M,Ohtsuka T

更新日期：2011-06-01 00:00:00

abstract：:Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, ...

journal_title：Journal of biochemistry

authors： Matsumura K,Hori S,Sugiura H,Shimizu T

更新日期：1990-10-01 00:00:00

abstract：:Phase transition was detected by a fluorescence polarization technique in the membrane of Thermus thermophilus HB8: it was found to be a function of cell growth temperature when the cell growth temperature was varied between 50-80 degrees C. A systematic relation between the phase transition temperature and the growth...

journal_title：Journal of biochemistry

authors： Wakayama N,Oshima T

更新日期：1978-06-01 00:00:00

abstract：:The interactions of substoichiometric TNP-ATP and F1-ATPase from Escherichia coli (EF1) were examined and compared with those in the case of mitochondrial F1-ATPase (MF1) and F1-ATPase from thermophilic Bacillus PS3 (TF1). EF1 hydrolyzed substoichiometric TNP-ATP faster than TF1 or MF1, although some 20% of the TNP-AT...

journal_title：Journal of biochemistry

authors： Muneyuki E,Hisabori T,Sasayama T,Mochizuki K,Yoshida M

更新日期：1996-11-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:The presence of two genomic DNA regions encoding malate synthase (MS) was shown by Southern blot analysis of the genomic DNA from an n-alkane-assimilating yeast, Candida tropicalis, using a partial MS cDNA probe, in accordance with the fact that two types of partial MS cDNAs have previously been isolated. This was als...

journal_title：Journal of biochemistry

authors： Hikida M,Atomi H,Fukuda Y,Aoki A,Hishida T,Teranishi Y,Ueda M,Tanaka A

更新日期：1991-12-01 00:00:00

abstract：:Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet react...

journal_title：Journal of biochemistry

authors： Takahashi C,Nakakita S,Hase S

更新日期：2003-07-01 00:00:00

abstract：:Poly(A) polymerase [EC 2.7.7.19] was highly purified from beef liver nuclei by the use of column chromatographies on heparin-Sepharose 4B and Blue Dextran-Sepharose 4B. The purified enzyme showed one major protein band of the molecular weight of 57,000 in SDS polyacrylamide gel electrophoresis, which agreed with the m...

journal_title：Journal of biochemistry

authors： Ohyama Y,Fukami H,Ohta T

更新日期：1980-08-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:Previously, we reported that proteasomes (large multi-protease complexes) are present in a latent state in a variety of eukaryotic cells, and can be activated by treatment with various compounds such as sodium dodecyl sulfate (SDS) or poly-lysine (Tanaka et al. (1988) J. Biol. Chem. 263, 16209-16217). In the present s...

journal_title：Journal of biochemistry

authors： Tanaka K,Yoshimura T,Ichihara A

更新日期：1989-09-01 00:00:00

abstract：:Dopamine D2 receptor binding subunits of the porcine anterior pituitary were visualized by autoradiography following photoaffinity labeling with [125I]N-azidophenethylspiperone and sodium dodecyl-sulfate polyacrylamide gel electrophoresis. The ligand binding subunit comprising the pituitary D2 dopamine receptor migrat...

journal_title：Journal of biochemistry

authors： Jarvie KR,Niznik HB

更新日期：1989-07-01 00:00:00

abstract：:Kinetic studies of the two beta-glucosidase isozymes, GB-1 and GB-2, which were from the culture filtrate of a phytopathogenic fungus Pyricularia oryzae, revaled that the latter isozyme was an allosteric protein with two substrate binding sites. The homotropic effects of o- and m-nitrophenyl-beta-D-glucosides on GB-2 ...

journal_title：Journal of biochemistry

authors： Hirayama T,Nagayama H,Matsuda K

更新日期：1980-04-01 00:00:00

abstract：:The cDNAs encoding the precursors of cystatin SN, cystatin S, and two mutants of cystatin S (-18R-->W; 117R-->W) were expressed in Escherichia coli JM109 with isopropyl-beta-D-thio-galactoside (IPTG) induction. Premature cystatin S with the original signal [-20MARPLCTLLLLMATLAGALA] was processed and a large amount of ...

journal_title：Journal of biochemistry

authors： Saitoh E,Isemura S

更新日期：1994-08-01 00:00:00

abstract：:Dithiothreitol or glutathione, essential for amino acid incorporation into polypeptides in cell-free systems, inhibited glucosamine incorporation into ovalbumin, a glycoprotein containing about three residues of N-acetylucosamine and about five residues of mannose. However, the thiol was necessary for mannose incorpor...

journal_title：Journal of biochemistry

authors： Nakayama T,Narita K,Ogata K

更新日期：1976-05-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:alphaB-Crystallin, a member of the small heat shock protein (sHSP) family, is expressed in various tissues including lens, heart, and skeletal muscle. Previously we identified the gene of HSPB2, another member of the sHSP family, located 1-kb upstream of the alphaB-crystallin gene in a head-to-head manner. In the pres...

journal_title：Journal of biochemistry

authors： Ijichi N,Tsujimoto N,Iwaki T,Fukumaki Y,Iwaki A

更新日期：2004-03-01 00:00:00

abstract：:A glutathione S-transferase isozyme which is absent in normal rat liver has been isolated from the hereditary hyperbilirubinuria rat liver cytosol. The enzyme was purified to apparent homogeneity by GSH-affinity chromatography and HPLC on CM-Sepharose CL-6B. It is a heterodimer of two non-identical subunits, i.e., sub...

journal_title：Journal of biochemistry

authors： Igarashi T,Tsuchiya T,Satoh T

更新日期：1991-01-01 00:00:00

abstract：:2-Amino-4-methylphenol was converted to a brownish yellow material by the lysates of human erythrocytes or purified human hemoglobin. The reaction proceeded oxidatively, coupled with the oxidation of hemoglobin. The major component of the brownish yellow material produced by oxidative condensation of 2-amino-4-methylp...

journal_title：Journal of biochemistry

authors： Tomoda A,Arisawa M,Koshimura S

更新日期：1991-12-01 00:00:00

abstract：:Carboxyl groups of native human fibrinogen were modified with glycine methyl ester and 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. It seemed likely that the modification occurred stepwise. Approximately 26% of the carboxyl groups of fibrinogen was modified finally. The modified fibrinogen had no interaction with cat...

journal_title：Journal of biochemistry

authors： Kurioka S,Inoue F,Matsuda M

更新日期：1975-11-01 00:00:00

abstract：:beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

journal_title：Journal of biochemistry

authors： D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

更新日期：1999-09-01 00:00:00

abstract：:We previously identified a human cDNA encoding a novel receptor tyrosine kinase, termed Sky, which is predominantly expressed in the brain and has a unique extracellular domain consisting of two immunoglobulin (Ig)-like and two fibronectin type III (FN III) motifs. In attempts to define the functional role of the Sky ...

journal_title：Journal of biochemistry

authors： Ohashi K,Honda S,Ichinomiya N,Nakamura T,Mizuno K

更新日期：1995-06-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...

journal_title：Journal of biochemistry

authors： Okazaki N,Hazeki K,Izumi T,Nigorikawa K,Hazeki O

更新日期：2011-03-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00