Activation of SoxR-dependent transcription in Pseudomonas aeruginosa.

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:Bacterial flagellar motors exploit the electrochemical potential gradient of a coupling ion as energy source and are composed of stator and rotor proteins. Vibrio alginolyticus has a Na(+)-driven motor and its stator is composed of PomA and PomB. Recently, we isolated increased motility strains (sp1-sp4) from the PomA...

journal_title：Journal of biochemistry

authors： Terashima H,Terauchi T,Ihara K,Nishioka N,Kojima S,Homma M

更新日期：2013-08-01 00:00:00

abstract：:In a previous study, we purified PAF-acetylhydrolase, which converts PAF to an inactive metabolite, lysoPAF, from peritoneal fluid of guinea pigs subjected to experimental endotoxin shock and found that this purified enzyme had similar biochemical properties to the plasma enzyme [Karasawa, K., Yato, M., Setaka, M., an...

journal_title：Journal of biochemistry

authors： Karasawa K,Kuge O,Kawasaki K,Nishijima M,Nakano Y,Tomita M,Yokoyama K,Setaka M,Nojima S

更新日期：1996-10-01 00:00:00

abstract：:Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

journal_title：Journal of biochemistry

authors： Murase T,Yamada N,Akanuma Y,Ohsawa N

更新日期：1982-09-01 00:00:00

abstract：:D-Erythrulose reductase of beef liver was crystallized from ammonium sulfate solution at pH 8.17. The crystals are needle-shaped. The enzyme protein contains 851 amino acid residues per mole of the enzyme: Lys28, His11, Arg52, Asp79, Thr58, Ser56, Glu68, Pro20, Gly80, Ala107, Val112, Met24, Ile31, Leu88, Tyr7, Phe22, ...

journal_title：Journal of biochemistry

authors： Uehara K,Tanimoto T

更新日期：1975-09-01 00:00:00

abstract：:Many membrane proteins such as ion channels are oligomers, but the determinants of the degree of oligomerization are not fully understood. Mechanosensitive channel with large conductance (MscL), which is ubiquitous in bacteria, is a homopentamer with two transmembrane helices and a cytoplasmic helix in each subunit. T...

journal_title：Journal of biochemistry

authors： Ando C,Liu N,Yoshimura K

更新日期：2015-08-01 00:00:00

abstract：:It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...

journal_title：Journal of biochemistry

authors： Yamato K,Handa S,Yamakawa T

更新日期：1975-12-01 00:00:00

abstract：:A number of L-amino acids and derivatives were tested as substrates for the purified Pseudomonas L-phenylalanine oxidase. The reaction products of these amino acids were analyzed by high performance liquid chromatography and the kinetic properties of the reactions were partially characterized. In addition to L-phenyla...

journal_title：Journal of biochemistry

authors： Koyama H

更新日期：1984-08-01 00:00:00

abstract：:Autoactivation of the proenzyme form of a subunit of the first component (C1r) was performed in the presence and absence of diisopropyl fluorophosphate (DFP). The time-course of autoactivation of zymogen C1r followed a sigmoidal curve and was accelerated by addition of the enzyme C1r and by increasing the concentratio...

journal_title：Journal of biochemistry

authors： Kasahara Y,Odai H,Takahashi K,Nagasawa S,Koyama J

更新日期：1985-01-01 00:00:00

abstract：:Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

journal_title：Journal of biochemistry

authors： Mikami B,Nomura K,Morita Y

更新日期：1983-07-01 00:00:00

abstract：:In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with...

journal_title：Journal of biochemistry

authors： Nakamura Y,Oda K,Nakada S

更新日期：1991-09-01 00:00:00

abstract：:Calmodulin-dependent protein kinase IV (CaM-kinase IV) kinase was recently discovered in the rat brain by its activity to activate the inactive recombinant CaM-kinase IV expressed in Escherichia coli [Okuno, S. and Fujisawa, H. (1993) J. Biochem. 114, 167-170]. In the present study, CaM-kinase IV kinase was purified a...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：1994-10-01 00:00:00

abstract：:A gene for a methionine aminopeptidase (MAP; EC 3.4.11.18), which catalyzes the removal of amino-terminal methionine from the growing peptide chain on the ribosome, has been cloned from the hyperthermophilic Archaeon, Pyrococcus furiosus, by a novel method effectively using its cosmid protein library, sequenced and ex...

journal_title：Journal of biochemistry

authors： Tsunasawa S,Izu Y,Miyagi M,Kato I

更新日期：1997-10-01 00:00:00

abstract：:Studies on human cell hybrids of a cervical carcinoma cell line, HeLa, and normal fibroblasts have indicated that the tumorigenicity of these cells is under the control of a putative tumor suppressor on chromosome 11, although the nature of this suppressor remains unknown. We examined the expression of caveolin-1, a p...

journal_title：Journal of biochemistry

authors： Suzuki T,Suzuki Y,Hanada K,Hashimoto A,Redpath JL,Stanbridge EJ,Nishijima M,Kitagawa T

更新日期：1998-08-01 00:00:00

abstract：:The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

journal_title：Journal of biochemistry

authors： Takenaka H,Ikehara M,Tonomura Y

更新日期：1976-12-01 00:00:00

abstract：:The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous w...

journal_title：Journal of biochemistry

authors： Yoon SS,Park SH,Kim YC,Shin M,Chong CK,Choi JD

更新日期：2008-10-01 00:00:00

abstract：:Glutamine synthetase from a Gram-positive acid-fast bacterium, Mycobacterium smegmatis, was purified to homogeneity from cells grown with glycerol-bouillon medium. Electron micrographs of the enzyme revealed a dodecameric arrangement of its subunits in two superimposed hexagonal rings, similar to the structure of glut...

journal_title：Journal of biochemistry

authors： Kimura K,Yagi K,Matsuoka K

更新日期：1984-06-01 00:00:00

abstract：:A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

journal_title：Journal of biochemistry

authors： Ohnishi M,Yamashita T,Hiromi K

更新日期：1977-01-01 00:00:00

abstract：:Three alkaline DNases, A, B, and C, with preference for the digestion of double-stranded DNA (dsDNA) were partially purified from microplasmodia of Physarum polycephalum. They were very similar but differed in their isoelectric points. These were pH 5.8 for DNase A, 7.1 for DNase B, and 9.1 for DNase C. All three enzy...

journal_title：Journal of biochemistry

authors： Waterborg JH,Kuyper CM

更新日期：1980-02-01 00:00:00

abstract：:The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein of 286 amino acids. The amino acid sequence shared approximately 80-90% similarity to the Bacillus ...

journal_title：Journal of biochemistry

authors： Ertunga NS,Colak A,Belduz AO,Canakci S,Karaoglu H,Sandalli C

更新日期：2007-06-01 00:00:00

abstract：:To investigate the role of the C-terminal region of barley beta-amylase, plasmid delta 54 was constructed with an expression vector (pBETA92) of barley beta-amylase by site-directed mutagenesis. Escherichia coli JM109 harboring plasmid delta 54 was expected to express delta 54 beta-amylase in which 54 amino acid resid...

journal_title：Journal of biochemistry

authors： Yoshigi N,Sahara H,Koshino S

更新日期：1995-01-01 00:00:00

abstract：:Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodec...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-10-01 00:00:00

abstract：:A novel fucose-containing neutral glycosphingolipid (GL-5) was purified from the eggs of the sea urchin, Hemicentrotus pulcherrimus. The chemical structure was determined to be Fuc alpha 1-3GalNAc beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-4Glc beta 1-1Cer by methylation analysis, partial acid hydrolysis, fast atom bombardm...

journal_title：Journal of biochemistry

authors： Kubo H,Jiang GJ,Irie A,Suzuki M,Inagaki F,Hoshi M

更新日期：1992-08-01 00:00:00

abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...

journal_title：Journal of biochemistry

authors： Katayama E,Wakabayashi T,Reinach F,Masaki T,Fischman DA

更新日期：1984-03-01 00:00:00

abstract：:The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

journal_title：Journal of biochemistry

authors： Inoue H,Takahashi S,Fukui K,Miyake Y

更新日期：1991-10-01 00:00:00

abstract：:Activation of antiviral innate immunity is triggered by cellular pattern recognition receptors. Retinoic acid inducible gene-I (RIG-I)-like receptors (RLRs) detect viral non-self RNA in cytoplasm of virus-infected cells and play a critical role in the clearance of the invaded viruses through production of antiviral cy...

journal_title：Journal of biochemistry

authors： Yoneyama M,Jogi M,Onomoto K

更新日期：2016-03-01 00:00:00

abstract：:3-Ketovalidoxylamine A C-N lyase was purified about 900-fold from the cell-free extract of Flavobacterium saccharophilum by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate (SDS) polyacry...

journal_title：Journal of biochemistry

authors： Takeuchi M,Asano N,Kameda Y,Matsui K

更新日期：1985-12-01 00:00:00

abstract：:The expression of the human SREBP-2 gene is transcriptionally regulated in a cooperative manner by sterol regulatory element-binding proteins (SREBPs) and the general transcription factor NF-Y [Sato, R., Inoue, J., Kawabe, Y., Kodama, T., Takano, T., and Maeda, M. (1996) J. Biol. Chem. 271, 26461-26464]. To understand...

journal_title：Journal of biochemistry

authors： Inoue J,Sato R,Maeda M

更新日期：1998-06-01 00:00:00

abstract：:Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. The purified enzyme was very labile when incubated either at 37 degrees C or 0 degrees C, and lost its ac...

journal_title：Journal of biochemistry

authors： Natori Y,Karasawa K,Arai H,Tamori-Natori Y,Nojima S

更新日期：1983-02-01 00:00:00