Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen.

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00

abstract：:The nucleotide sequence of gene PBII encoding salivary proline-rich protein P-B was determined. PBII is 7.1 kb long and contains 3 exons. PBII exhibits considerable nucleotide sequence homology not only in exons but also in introns with PBI (accession number D89501), the gene whose nucleotide sequence was determined p...

journal_title：Journal of biochemistry

authors： Isemura S

更新日期：2000-03-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:We studied the mechanism of formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study. The hydroxyl radical is also generated through Fenton t...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Tero-Kubota S,Yamamoto Y,Kato M,Nakano M,Yagi K,Ogino K

更新日期：2003-12-01 00:00:00

abstract：:We generated and characterized novel antibodies specific for a cleavage site of human caspase-8/FLICE and its substrate, FLICE-like inhibitory protein (FLIP). The synthetic peptides used as immunogens were CQGDNYQKGIPVETD (#791) and VSEGQLEDSSLLEVD (#1342), which corresponded to cleaved regions of N-terminal fragments...

journal_title：Journal of biochemistry

authors： Niikura Y,Nonaka T,Imajoh-Ohmi S

更新日期：2002-07-01 00:00:00

abstract：:Thrombomodulin (TM) is a thrombin receptor on the surface of endothelial cells that converts thrombin from a procoagulant to an anticoagulant. Thrombin promotes invasion by various tumor cells, and positive or negative correlations are found between the expression of TM and tumorigenesis in some patients. In this stud...

journal_title：Journal of biochemistry

authors： Niimi S,Harashima M,Takayama K,Hara M,Hyuga M,Seki T,Ariga T,Kawanishi T,Hayakawa T

更新日期：2005-05-01 00:00:00

abstract：:Studies were carried out on the delta 5-desaturation reaction in ergosterol biosynthesis with a particulate fraction of cell-free extract of yeast. A reduced pyridine nucleotide coenzyme and molecular oxygen were required for the reaction. It was shown that the enzyme activity is located in a fraction corresponding to...

journal_title：Journal of biochemistry

authors： Osumi T,Nishino T,Katsuki H

更新日期：1979-03-01 00:00:00

abstract：:An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against ...

journal_title：Journal of biochemistry

authors： Iwasaki A,Suda M,Nakao H,Nagoya T,Saino Y,Arai K,Mizoguchi T,Sato F,Yoshizaki H,Hirata M

更新日期：1987-11-01 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:An actin-depolymerizing marine natural product, mycalolide B, and a related compound, kabiramide D, were labeled with biocytin, a biotin derivative, and used to specify target molecules in cultured rat 3Y1 fibroblasts. Mycalolide B exhibited the ability to bind to various intracellular proteins, probably through the M...

journal_title：Journal of biochemistry

authors： Wada S,Matsunaga S,Saito S,Fusetani N,Watabe S

更新日期：1998-05-01 00:00:00

abstract：:Alginate, which is an anionic polysaccharide, is widely distributed in the cell wall of brown algae. Alginate and the products of its degradation (oligosaccharides) are used in stabilizers, thickeners and gelling agents, especially in the food industry. The degradation of alginate generally involves a combination of s...

journal_title：Journal of biochemistry

authors： Yagi H,Fujise A,Itabashi N,Ohshiro T

更新日期：2018-04-01 00:00:00

abstract：:Aldehyde reductases from several mammalian and avian tissues transferred the pro-4R hydrogen of NADPH to the substrate, whereas the stereospecificity of carbonyl reductases was not uniform being correlated with the ability to catalyze the oxidoreduction of hydroxysteroids. ...

journal_title：Journal of biochemistry

authors： Nakayama T,Hara A,Kariya K,Sawada H

更新日期：1985-10-01 00:00:00

abstract：:Thymidine kinase [ATP: thymidine 5'-phosphotransferase, EC 2.7.1.21] has been purified more than 3,500 fold from microplasmodia of Physarum polycephalum. Properties of the enzyme were determined on preparations purified 1,400 fold. Thymidine was transformed to dTMP while a stoichiometric quantity of ATP was transforme...

journal_title：Journal of biochemistry

authors： Chraibi R,Wright M

更新日期：1983-02-01 00:00:00

abstract：:NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

journal_title：Journal of biochemistry

authors： Shioi Y,Takamiya K,Nishimura M

更新日期：1976-02-01 00:00:00

abstract：:β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to th...

journal_title：Journal of biochemistry

authors： Okabe T,Miyajima T,Nakagawa K,Tsukamoto S,Fujiwara K,Ikeguchi M

更新日期：2014-11-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated plate...

journal_title：Journal of biochemistry

authors： Takuwa Y,Takuwa N,Sugimoto N

更新日期：2002-06-01 00:00:00

abstract：:Calf thymus DNA polymerases alpha and beta [EC 2.7.7.7] and terminal deoxynucleotidyl transferase [EC 2.7.7.31] were analyzed on two-dimensional gel slabs. DNA polymerase beta appeared as a single spot on two-dimensional gel at the position of 40,000 daltons and pI 8.0 using non-equilibrium pH gradient gel electrophor...

journal_title：Journal of biochemistry

authors： Koiwai O,Masaki S,Kaneda T,Yoshida S

更新日期：1982-08-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:A sugar autoanalyzer was used on a preparative scale to resolve a gluco-oligosaccharide mixture. In this way the components of the following mixtures were resolved: O-alpha-D-glucopyranosyl-(1-3)-O-[alpha-D-glucopyranosyl-(1-6)]-D-glucose (1), O-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl-(1-3)-D-glucose (2)...

journal_title：Journal of biochemistry

authors： Torii M,Alberto BP,Tanaka S,Tsumuraya Y,Misaki A,Sawai T

更新日期：1979-03-01 00:00:00

abstract：:The gizzard tropomyosin molecule is composed of two subunits at 1:1 molar ratio. Possible composites of the tropomyosin molecule are two kinds of homodimer (one for each subunit), a heterodimer of two subunits, or a mixture of heterodimer and homodimer(s). We tried to evaluate the native subunit composition of gizzard...

journal_title：Journal of biochemistry

authors： Nakamura S,Sakurai T,Nonomura Y

更新日期：1991-05-01 00:00:00

abstract：:A cell-free protein-synthesizing system active in initiation of translation of both endogenous mRNA and exogenous mRNA has been obtained from postmitochondrial supernatant (S-12) of the liver of ethionine-treated rats by adding reticulocyte ribosomal extract as a source of initiation factor. Formation of polysomes in ...

journal_title：Journal of biochemistry

authors： Hase M,Endo Y,Natori Y

更新日期：1982-05-01 00:00:00

abstract：:An ultraviolet absorption difference spectrum characteristic of the ionization change of a tyrosyl residue was observed on the binding of subtilisin BPN' with Streptomyces subtilisin inhibitor (SSI) at alkaline pH. This difference spectrum was considered to be induced by a pKa shift (from 9.7 to > or = 11.5) of a tyro...

journal_title：Journal of biochemistry

authors： Masuda-Momma K,Shimakawa T,Inouye K,Hiromi K,Kojima S,Kumagai I,Miura K,Tonomura B

更新日期：1993-12-01 00:00:00

abstract：:In our recent study on monoclonal antibodies (mAbs AHA-1-5) against glutaraldehyde (GA)-conjugated histamine (HA), we identified one mAb (AHA-2) which can detect neuronal HA in the rat brain with an immunocytochemistry method (ICC) [Fujiwara et al. (1999) J. Biochem. 126, 503-509]. In the present study the specificity...

journal_title：Journal of biochemistry

authors： Fujiwara K,Murata I,Yagisawa S,Tanabe T,Yabuuchi M,Sakakibara R,Tsuru D

更新日期：1999-12-01 00:00:00

abstract：:The sulfhydryl groups required for the catalytic activity of gramicidin S synthetase of Bacillus brevis and Escherichia coli isoleucyl tRNA synthetase were compared. In gramicidin S synthetase 2(GS 2), about four sulfhydryl groups react rapidly with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) or N-ethylmaleimide (NEM),...

journal_title：Journal of biochemistry

authors： Kanda M,Hori K,Kurotsu T,Miura S,Saito Y

更新日期：1984-09-01 00:00:00

abstract：:We have studied the protein composition of the pectoralis superficialis muscle of genetically dystrophic (New Hampshire line 413) and normal control (line 412) chickens by one- and two-dimensional gel electrophoresis. A protein, referred to hereafter as the 30 kDa abnormal protein, was specifically detected in the aff...

journal_title：Journal of biochemistry

authors： Kobayashi R,Toyoshima I,Masamune O,Tashima Y

更新日期：1990-01-01 00:00:00

abstract：:The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...

journal_title：Journal of biochemistry

authors： Katagiri M,Murakami H,Yabusaki Y,Sugiyama T,Okamoto M,Yamano T,Ohkawa H

更新日期：1986-10-01 00:00:00

abstract：:Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

journal_title：Journal of biochemistry

authors： Ogawa T,Shimohigashi Y,Ohno M

更新日期：1997-11-01 00:00:00

abstract：:The localization of GTP-binding protein (G-protein) subunits, Go alpha, Gi2 alpha and beta, in subcellular fractions of rat cerebral cortex was determined by means of immunoassays specific for the respective subunits. High concentrations of all three subunits were observed in both crude mitochondrial and microsomal fr...

journal_title：Journal of biochemistry

authors： Asano T,Nagahama M,Kato K

更新日期：1990-05-01 00:00:00

abstract：:A membrane protein insoluble in water was isolated by gel chromatography in the presence of 0.1% sodium dodecyl sulfate (SDS) from chromatophores of a photosynthetic bacterium, Rhodospirillum rubrum. This is one of the major membrane proteins of the chromatophore. The protein was found to bind about four grams of SDS ...

journal_title：Journal of biochemistry

authors： Miyake J,Ochiai-Yanagi S,Kasumi T,Takagi T

更新日期：1978-06-01 00:00:00