Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: a new lipocortin-like protein.

abstract：:The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphoryla...

journal_title：Journal of biochemistry

authors： Miyazawa K

更新日期：2013-01-01 00:00:00

abstract：:Studies on the solution structure dynamics of RNA/DNA are becoming crucially important. The phenomena of SSCP (single-strand conformation polymorphism), small RNA dynamics in a cell, and others can be related to the conformational changes of single-stranded (ss) RNAs/DNAs in solution. However, little is known about th...

journal_title：Journal of biochemistry

authors： Biyani M,Nishigaki K

更新日期：2005-10-01 00:00:00

abstract：:Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related...

journal_title：Journal of biochemistry

authors： Kato S,Tsuji M,Nakanishi Y,Suzuki S

更新日期：1980-03-01 00:00:00

abstract：:The activities of the digestive enzymes, maltase [EC 3.2.1.20], sucrase [EC 3.2.1.26], trehalase [EC 3.2.1.28], Leucine aminopeptidase [EC 3.4.11.1], and alkaline phosphatase [EC 3.1.3.1] were measured in various regions of the small intestine of rats. The activities of all these enzymes were much higher in the jejunu...

journal_title：Journal of biochemistry

authors： Saito M,Murakami E,Nishida T,Fujisawa Y,Suda M

更新日期：1975-09-01 00:00:00

abstract：:The structures of the N-linked oligosaccharides on recombinant human hepatocyte growth factor (rh-HGF) expressed by Chinese hamster ovary (CHO) cells were studied by two-dimensional sugar mapping. The oligosaccharides released from the glycopeptides by peptide: N-glycosidase F (PNGase F) treatment were tagged with 2-a...

journal_title：Journal of biochemistry

authors： Hara H,Nakae Y,Sogabe T,Ihara I,Ueno S,Sakai H,Inoue H,Shimizu S,Nakamura T,Shimizu N

更新日期：1993-07-01 00:00:00

abstract：:We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole...

journal_title：Journal of biochemistry

authors： Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo T

更新日期：2007-04-01 00:00:00

abstract：:The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG1 in the expression...

journal_title：Journal of biochemistry

authors： Inayoshi Y,Kaneoka H,Machida Y,Terajima M,Dohda T,Miyake K,Iijima S

更新日期：2005-10-01 00:00:00

abstract：:Papain [EC 3.4.22.2] polymerizes readily upon treatment with tetranitromethane (TNM) by forming intermolecular covalent linkages through its tyrosine residues (Tsukamoto, S. & Ohno, M. (1974) J. Biochem. 75, 1377-1380). Polymerization occurred optimally at pH 9.0 with S-sulfenylsulfonate papain. Circular dichroic spec...

journal_title：Journal of biochemistry

authors： Tsukamoto S,Ohno M

更新日期：1978-12-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:Mitochondria exhibiting cyanide-resistant respiration were isolated from Hansenula anomala which had been incubated in the presence of antimycin A to induce cyanide-resistant respiration. The cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin A or myxothiazol, suggesting that the bra...

journal_title：Journal of biochemistry

authors： Sakajo S,Minagawa N,Komiyama T,Yoshimoto A

更新日期：1990-08-01 00:00:00

abstract：:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamo-Yamada F,Cho S,Sugano M

更新日期：1980-05-01 00:00:00

abstract：:Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. Carbohydrate analyses showed that the catalase was a glycoprotein containing a...

journal_title：Journal of biochemistry

authors： Kikuchi-Torii K,Hayashi S,Nakamoto H,Nakamura S

更新日期：1982-11-01 00:00:00

abstract：:Neurotrophins such as NGF promote neuronal survival and differentiation via the cell surface TrkA neurotrophin receptor. Compounds with neurotrophic actions that are low in molecular weight and can permeate the blood-brain barrier are promising therapeutic agents against neurodegenerative diseases such as Alzheimer's ...

journal_title：Journal of biochemistry

authors： Kosaka K,Mimura J,Itoh K,Satoh T,Shimojo Y,Kitajima C,Maruyama A,Yamamoto M,Shirasawa T

更新日期：2010-01-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:Troponin is the regulatory protein of striated muscle. Without Ca2+, the contraction of striated muscle is inhibited. Binding of Ca2+ to troponin activates contraction. The location of troponin on the thin filaments and its relation to the regulatory mechanism has been unknown, though the Ca2+-induced dislocation of t...

journal_title：Journal of biochemistry

authors： Ishikawa T,Wakabayashi T

更新日期：1999-07-01 00:00:00

abstract：:p40(phox) of the phagocyte NADPH oxidase forms a complex with p67(phox) in cytosol, and coincidentally decreases in patients who lack p67(phox). Here we investigated the mode of translocation of p40(phox) to the membrane, its cytoskeletal localization on activation of the NADPH oxidase, and the dependency of its expre...

journal_title：Journal of biochemistry

authors： Tsunawaki S,Yoshikawa K

更新日期：2000-11-01 00:00:00

abstract：:alphaB-Crystallin, a member of the small heat shock protein (sHSP) family, is expressed in various tissues including lens, heart, and skeletal muscle. Previously we identified the gene of HSPB2, another member of the sHSP family, located 1-kb upstream of the alphaB-crystallin gene in a head-to-head manner. In the pres...

journal_title：Journal of biochemistry

authors： Ijichi N,Tsujimoto N,Iwaki T,Fukumaki Y,Iwaki A

更新日期：2004-03-01 00:00:00

abstract：:Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-compl...

journal_title：Journal of biochemistry

authors： Baba S,Takahashi K,Noguchi S,Takaku H,Koyanagi Y,Yamamoto N,Kawai G

更新日期：2005-11-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:The effects of intra- and extravesicular calcium and magnesium ions on the hydrolysis of the phosphoenzyme (EP) intermediate formed in the reaction of Ca2+,Mg2+-dependent ATPase of the sarcoplasmic reticulum were investigated. The rate constants of EP hydrolysis were measured under conditions that allowed a single tur...

journal_title：Journal of biochemistry

authors： Daiho T,Takisawa H,Yamamoto T

更新日期：1985-02-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP3) releases Ca2+ from the non-mitochondrial Ca2+ store site of various types of cells. To study the mechanisms of the Ca2+ release from the store site, the effect of InsP3 on the passive Ca2+ release and influx, and the active Ca2+ uptake in the presence of oxalate, was examined using...

journal_title：Journal of biochemistry

authors： Hirata M,Kukita M,Sasaguri T,Suematsu E,Hashimoto T,Koga T

更新日期：1985-06-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

journal_title：Journal of biochemistry

authors： Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

更新日期：2001-08-01 00:00:00

abstract：:To investigate the function of the gamma-carboxyglutamic acid (Gla) residues of factor IXa in the activation of factor X, a new species of bovine factor IXa, designated "factor IXa beta'," and its corresponding Gla-domainless form, designated "Gla-domainless factor IXa beta'," were prepared under controlled conditions...

journal_title：Journal of biochemistry

authors： Morita T,Fukudome K,Miyata T,Iwanaga S

更新日期：1991-12-01 00:00:00

abstract：:The inhibition of tau fibrillation is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. As a series of studies on inhibiting the transition of soluble monomeric tau into mature fibril, the effect of Tyr310 residue in the third repeat (R3) of the microtubule-binding domain (MBD) on th...

journal_title：Journal of biochemistry

authors： Nishiura C,Takeuchi K,Minoura K,Sumida M,Taniguchi T,Tomoo K,Ishida T

更新日期：2010-03-01 00:00:00

abstract：:Hydrostatic pressure-induced morphological and physiochemical changes in monomeric myosin were investigated. The turbidity of a myosin solution increased after release of pressure, indicating aggregation of the molecules. Some molecules were single headed after exposure to 100-200 MPa, in contrast with an intact molec...

journal_title：Journal of biochemistry

authors： Yamamoto K,Yoshida Y,Morita J,Yasui T

更新日期：1994-07-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:The phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme catalyzes reversibly the intra-molecular phosphoryl interconverting reaction of mannose-6-phosphate and mannose-1-phosphate or glucose-6-phosphate and glucose-1-phosphate. Glucose-6-phosphate and glucose-1-phosphate are known to be utilized for energy metaboli...

journal_title：Journal of biochemistry

authors： Akutsu J,Zhang Z,Tsujimura M,Sasaki M,Yohda M,Kawarabayasi Y

更新日期：2005-08-01 00:00:00

abstract：:Saccharomyces cerevisiae has three distinct citrate synthases, two located in mitochondria (mature Cit1p and Cit3p) and one in peroxisomes (mature Cit2p). While the precursor of the major mitochondrial enzyme, Cit1p, has a signal for mitochondrial targeting at its N-terminus (MTS), Cit2p has one for peroxisomal target...

journal_title：Journal of biochemistry

authors： Lee JG,Cho SP,Lee HS,Lee CH,Bae KS,Maeng PJ

更新日期：2000-12-01 00:00:00

abstract：:The effects of polyamines on the breakdown of synthetic polynucleotides [poly(A), poly(C), and poly(U)] by E. coli ribonuclease I [ribonucleate 3'-oligonucleotidohydrolase, EC 3.1.4.23] and ribonuclease II [EC 3.1.4.1] have been studied. The degradation of poly(C) by RNase II was stimulated by spermine and spermidine,...

journal_title：Journal of biochemistry

authors： Kumagai H,Igarashi K,Yoshikawa M,Hirose S

更新日期：1977-02-01 00:00:00