Different buffer effects in selecting HM-1 killer toxin single-chain fragment variable anti-idiotypic antibodies.

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...

journal_title：Journal of biochemistry

authors： Kohno K

更新日期：2010-01-01 00:00:00

abstract：:A cysteine protease inhibitor with an apparent Mr = 12,600, designated limulus (L)-cystatin, was isolated from hemocyte lysates of the Japanese horseshoe crab (Tachypleus tridentatus), using two steps of chromatography, including dextran sulfate-agarose, and carboxymethylated papain-agarose. L-cystatin inhibits amidol...

journal_title：Journal of biochemistry

authors： Agarwala KL,Kawabata S,Hirata M,Miyagi M,Tsunasawa S,Iwanaga S

更新日期：1996-01-01 00:00:00

abstract：:Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

journal_title：Journal of biochemistry

authors： Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

更新日期：2003-12-01 00:00:00

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:Ras is a signal-transducing, guanine nucleotide-binding protein for various membrane receptors including tyrosine kinase receptors. Ras participates in the regulation of cell proliferation, differentiation, and morphology. Activated ras oncogenes have been identified in various forms of human cancer including epitheli...

journal_title：Journal of biochemistry

authors： Yamamoto T,Taya S,Kaibuchi K

更新日期：1999-11-01 00:00:00

abstract：:We previously showed that fatty liver was easily induced in suncus by starvation and that the plasma level of apolipoprotein B (apo B) was very low. There are three possible explanations for the low level of apo B in the animals: low synthetic rate, low secretion rate, and rapid catabolism in the circulation of apo B....

journal_title：Journal of biochemistry

authors： Nagayoshi A,Matsuki N,Saito H,Tsukamoto K,Kaneko K,Wakashima M,Kinoshita M,Yamanaka M,Teramoto T

更新日期：1995-04-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00

abstract：:We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion fragments of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universa...

journal_title：Journal of biochemistry

authors： Kang DC,Takeshige K,Minakami S

更新日期：1990-12-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00

abstract：:To establish a system for overproduction of the ferrochelatase [EC 4.99.1.1] from Escherichia coli, a plasmid designated pFC3 was constructed. The 35-kDa protein was accumulated in E. coli DH5 alpha cells that harbored pFC3 to a level equal to approximately 9% of the total protein (roughly 50 mg/liter) upon thermal in...

journal_title：Journal of biochemistry

authors： Miyamoto K,Kanaya S,Morikawa K,Inokuchi H

更新日期：1994-03-01 00:00:00

abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...

journal_title：Journal of biochemistry

authors： Isemura S,Saitoh E,Ito S,Isemura M,Sanada K

更新日期：1984-10-01 00:00:00

abstract：:The effects of various divalent cations on the Ca2+ uptake by microsomes from bovine aortic smooth muscle were studied. High concentrations (1 mM) of Co2+, Zn2+, Mn2+, Fe2+, and Ni2+ inhibited neither the Ca2+ uptake by the microsomes nor the formation of the phosphorylated intermediate (E approximately P) of the Ca2+...

journal_title：Journal of biochemistry

authors： Sumida M,Hamada M,Takenaka H,Hirata Y,Nishigauchi K,Okuda H

更新日期：1986-09-01 00:00:00

abstract：:The stress-related protein Stm1 interacts with ribosomes, and is implicated in repressing translation. Stm1 was previously studied both in vivo and in vitro by cell-free translation systems using crude yeast lysates, but its precise functional mechanism remains obscure. Using an in vitro reconstituted translation syst...

journal_title：Journal of biochemistry

authors： Hayashi H,Nagai R,Abe T,Wada M,Ito K,Takeuchi-Tomita N

更新日期：2018-03-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamo-Yamada F,Cho S,Sugano M

更新日期：1980-05-01 00:00:00

abstract：:Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was r...

journal_title：Journal of biochemistry

authors： Degawa M,Namiki M,Yoshimoto N,Makino M,Iwamoto M,Nemoto K,Hashimoto Y

更新日期：2003-06-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:Transient and steady state kinetics were studied in the interactions of ATP with acto-H-meromyosin reconstituted from bovine arterial heavy-meromyosin (HMM) and rabbit skeletal muscle F-actin. The results showed that the rate of dissociation of the hybrid acto-HMM induced by ATP was slower than the rate of the fluores...

journal_title：Journal of biochemistry

authors： Takeuchi K,Tonomura Y

更新日期：1978-08-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:The denaturation of lysozyme and ribonuclease A by guanidine hydrochloride was followed in the presence and absence of glycerol and sorbitol by means of circular dichroism measurements at 25 degrees C. The protein-solvent interactions in the presence of these polyols were also studied by means of density measurements,...

journal_title：Journal of biochemistry

authors： Gekko K,Ito H

更新日期：1990-04-01 00:00:00

abstract：:Seven ganglioside species were isolated and purified from erythrocyte membranes of Japanese cats by DEAE-Sephadex and Iatrobeads column chromatographies. The structures of these gangliosides were determined as Gmi(NeuGc), Gm3(NeuAc), GM3(NeuGc), GD3(NeuGc), GD3(NeuGc comes from NeuAc), GT3(NeuGc), and another GM3 cont...

journal_title：Journal of biochemistry

authors： Ando N,Yamakawa T

更新日期：1982-03-01 00:00:00

abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

journal_title：Journal of biochemistry

authors： Maeng JH,Yoon JB

更新日期：1998-07-01 00:00:00

abstract：:We examined the signaling mechanisms involved in the differentiation-inducing activity of lupeol toward B16 2F2 melanoma cells. alpha-Melanocyte stimulating hormone (alpha-MSH), forskolin and dibutyryl cAMP, which are believed to be cAMP-elevating agents and analogues, enhanced lupeol-induced B16 2F2 cell differentiat...

journal_title：Journal of biochemistry

authors： Hata K,Hori K,Takahashi S

更新日期：2003-09-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:L-selectin is a cell adhesion molecule mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells expressing L-selectin ligands. In addition to its action in adhesion, an intracellular signaling role for L-selectin has been recognized. Its cytoplasmic domain is involved in signal tr...

journal_title：Journal of biochemistry

authors： Chen C,Ba X,Xu T,Cui L,Hao S,Zeng X

更新日期：2006-08-01 00:00:00

abstract：:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka S

更新日期：1989-05-01 00:00:00

abstract：:3-O-Methyl hexosamine was found for the first time and characterized as one of the sugar components of oyster glycolipid. The alditol acetate of this sugar was identified as 3-O-methyl-N-acetylgalactosaminitol acetate by comparing its retention time on gas chromatography and mass spectrum with those of the authentic s...

journal_title：Journal of biochemistry

authors： Matsubara T,Hayashi A

更新日期：1978-04-01 00:00:00