Sea urchin protease specific to the SPKK motif in histone.

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:The role that interaction with laminin may play in Leishmania donovani infection was investigated. Binding of (125)I-radiolabeled laminin, in a liquid-phase assay, by the parasite was rapid, saturable, specific, reversible, and of high affinity. Using a Western blotting procedure, a 67 kDa laminin-binding protein (LBP...

journal_title：Journal of biochemistry

authors： Bandyopadhyay K,Karmakar S,Ghosh A,Das PK

更新日期：2001-07-01 00:00:00

abstract：:We examined the signaling mechanisms involved in the differentiation-inducing activity of lupeol toward B16 2F2 melanoma cells. alpha-Melanocyte stimulating hormone (alpha-MSH), forskolin and dibutyryl cAMP, which are believed to be cAMP-elevating agents and analogues, enhanced lupeol-induced B16 2F2 cell differentiat...

journal_title：Journal of biochemistry

authors： Hata K,Hori K,Takahashi S

更新日期：2003-09-01 00:00:00

abstract：:Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1....

journal_title：Journal of biochemistry

authors： Komiya H,Takemura S

更新日期：1979-10-01 00:00:00

abstract：:A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

journal_title：Journal of biochemistry

authors： Oda S,Sato M,Toyoshima S,Osawa T

更新日期：1989-06-01 00:00:00

abstract：:Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

journal_title：Journal of biochemistry

authors： Narutaki S,Dunn BM,Oda K

更新日期：1999-01-01 00:00:00

abstract：:Frontal gel chromatography is a convenient and accurate method to obtain the free ligand concentration of a protein-ligand mixture. Because a large amount of sample (more than 6 ml) is required for the method, it has been rarely used for binding experiments. We have developed a system to carry out frontal gel chromato...

journal_title：Journal of biochemistry

authors： Honjo M,Ishida T,Horiike K

更新日期：1997-08-01 00:00:00

abstract：:Aldehyde reductases from several mammalian and avian tissues transferred the pro-4R hydrogen of NADPH to the substrate, whereas the stereospecificity of carbonyl reductases was not uniform being correlated with the ability to catalyze the oxidoreduction of hydroxysteroids. ...

journal_title：Journal of biochemistry

authors： Nakayama T,Hara A,Kariya K,Sawada H

更新日期：1985-10-01 00:00:00

abstract：:The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification. The purified preparation of the reductase showed an apparent molecular weight of 27,000 daltons. The reductase appeared to contain loosely-bound FAD as a prosthetic gr...

journal_title：Journal of biochemistry

authors： Kubota S,Yoshida Y,Kumaoka H

更新日期：1977-01-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:Rat brain type II (beta) protein kinase C (PKC) was phosphorylated by rat lung casein kinase II (CK-II). Neither type I (gamma) nor type III (alpha) PKC was significantly phosphorylated by CK-II. CK-II incorporated 0.2-0.3 mol of phosphate into 1 mol of type II PKC. This phosphate was located at the single seryl resid...

journal_title：Journal of biochemistry

authors： Tominaga M,Kitagawa Y,Tanaka S,Kishimoto A

更新日期：1991-10-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:Rat liver glucocorticoid receptor was partially purified and characterized for its hormone binding using selenite. Selenite at very low concentrations irreversibly inhibited the hormone binding. The concentration for half maximal inhibition was approximately 2.8 microM. The inhibition was restored by dithiothreitol. T...

journal_title：Journal of biochemistry

authors： Tashima Y,Terui M,Itoh H,Mizunuma H,Kobayashi R,Marumo F

更新日期：1989-03-01 00:00:00

abstract：:We recently described the cloning of putative human CMP-sialic acid transporter (hCST) cDNA [Ishida, N. et al. (1996) J. Biochem. 120, 1074-1078]. The hCST cDNA coded for a hydrophobic protein with an amino acid sequence showing a high degree of similarity (92% identity) to that of murine CMP-sialic acid transporter. ...

journal_title：Journal of biochemistry

authors： Ishida N,Ito M,Yoshioka S,Sun-Wada GH,Kawakita M

更新日期：1998-07-01 00:00:00

abstract：:Rho-kinase is a main player in the regulation of cytoskeletal events and a promising drug target in the treatment of both vascular and neurological disorders. Here we report the crystal structure of the Rho-kinase catalytic domain in complex with the specific inhibitor Y-27632. Comparison with the structure of PKA bou...

journal_title：Journal of biochemistry

authors： Yamaguchi H,Miwa Y,Kasa M,Kitano K,Amano M,Kaibuchi K,Hakoshima T

更新日期：2006-09-01 00:00:00

abstract：:We have isolated cDNAs and completed for the first time the primary structure for a novel collagenous chain that was partially characterized earlier and named alpha 1(Y) chain [Yoshioka, H. et al. (1992) Genomics 13, 884-886]. The size of the coding region was unexpectedly small compared with the length of the mRNA (>...

journal_title：Journal of biochemistry

authors： Inoguchi K,Yoshioka H,Khaleduzzaman M,Ninomiya Y

更新日期：1995-01-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:Class B scavenger receptor type I (SR-BI) is a phosphatidylserine (PS)-recognizing receptor of testicular Sertoli cells responsible for the phagocytosis of spermatogenic cells undergoing apoptosis. Here, we determined signal mediators that compose a signalling pathway for SR-BI-induced phagocytosis. Results of a yeast...

journal_title：Journal of biochemistry

authors： Osada Y,Sunatani T,Kim IS,Nakanishi Y,Shiratsuchi A

更新日期：2009-03-01 00:00:00

abstract：:3-Isopropylmalate dehydrogenase (IPMDH) is a dimeric enzyme with a strongly hydrophobic core that is composed of residues from four alpha-helices. We replaced Glu253, which is found in the hydrophobic core and is part of the subunit interface of the Bacillus subtilis (Bs) IPMDH, with several other amino acids to probe...

journal_title：Journal of biochemistry

authors： Ohkuri T,Yamagishi A

更新日期：2007-06-01 00:00:00

abstract：:Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

journal_title：Journal of biochemistry

authors： Nakanaga K,Hama K,Aoki J

更新日期：2010-07-01 00:00:00

abstract：:The binding of actin filaments to connectin, a muscle elastic protein, was investigated by means of turbidity and sedimentation measurements and electron microscopy. In the presence of less than 0.12 M KCl at pH 7.0, actin filaments bound to connectin. Long actin filaments formed bundles. Short actin filaments also ag...

journal_title：Journal of biochemistry

authors： Maruyama K,Hu DH,Suzuki T,Kimura S

更新日期：1987-06-01 00:00:00

abstract：:By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

journal_title：Journal of biochemistry

authors： Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

更新日期：1982-06-01 00:00:00

abstract：:High mobility group box 1 (HMGB1) protein is the most abundant chromatin-associated non-histone protein expressed in all nucleated eukaryotic cells. We examined the phosphorylation of mammalian HMGB1 by testing the ability of the cyclin-dependent kinase 5 (Cdk5) to use as substrates native protein, either unmodified o...

journal_title：Journal of biochemistry

authors： Ugrinova I,Pashev IG,Pasheva EA

更新日期：2011-05-01 00:00:00

abstract：:A hybridoma cell line producing mouse monoclonal antibody against pig kidney Na,K-ATPase was established. The antibody, named 38 (mAb38, IgG1), was purified from mouse ascites fluid by chromatography on a protein A-Sepharose column. Antigens immobilized on microplate wells with p-benzoquinone were used for titer assay...

journal_title：Journal of biochemistry

authors： Urayama O,Nagamune H,Nakao M,Hara Y,Sugiyama H,Sato K,Nakao T

更新日期：1985-07-01 00:00:00

abstract：:The amino acid sequence of the glycophorin from porcine erythrocyte membrane has been determined by Edman degradation. Porcine glycophorin is a polypeptide chain of 133 amino acid residues and contains 12 oligosaccharide units attached to the amino-terminal side of the molecule. Ten oligosaccharides are linked O-glyco...

journal_title：Journal of biochemistry

authors： Honma K,Tomita M,Hamada A

更新日期：1980-12-01 00:00:00

abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...

journal_title：Journal of biochemistry

authors： Hashinotsume M,Higashino K,Hada T,Yamamura Y

更新日期：1978-12-01 00:00:00

abstract：:We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion fragments of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universa...

journal_title：Journal of biochemistry

authors： Kang DC,Takeshige K,Minakami S

更新日期：1990-12-01 00:00:00

abstract：:Erucic acid was incorporated into cardiac phosphatidylserine and hepatic and cardiac sphingomyelin of male Sprague-Dawley rat. The fatty acid compositions of mitochondrial and microsomal phospholipids were similar in both liver and heart. The effect of a low fat diet and a diet containing erucic acid on the fatty acid...

journal_title：Journal of biochemistry

authors： Ishinaga M,Sato J,Kitagawa Y,Sugimoto E,Kito M

更新日期：1982-07-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

journal_title：Journal of biochemistry

authors： Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

更新日期：2001-08-01 00:00:00