Active site organization of bacterial type I fatty acid synthetase.

abstract：:The gene encoding subtilisin Amylosacchariticus from Bacillus subtilis var. amylosacchariticus was isolated and the entire nucleotide sequence of the coding sequence was determined. The deduced amino acid sequence revealed an N-terminal signal peptide and pro-peptide of 106 residues followed by the mature protein comp...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Oyama H,Honda T,Tone H,Takeshita T,Kamiyama T,Tsuru D

更新日期：1988-06-01 00:00:00

abstract：:Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecul...

journal_title：Journal of biochemistry

authors： Igarashi J,Hayashi N,Kikuchi G

更新日期：1976-11-01 00:00:00

abstract：:Midkine (MK) is a 13-kDa heparin-binding growth/differentiation factor, and its interaction with heparan sulfate proteoglycan is important in promotion of neurite outgrowth. This study was performed to reveal the species of syndecans that interact with MK during embryogenesis of the central nervous system in the rat. ...

journal_title：Journal of biochemistry

authors： Nakanishi T,Kadomatsu K,Okamoto T,Ichihara-Tanaka K,Kojima T,Saito H,Tomoda Y,Muramatsu T

更新日期：1997-02-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:Ornithine transcarbamylase (OTC) is one of the urea cycle enzymes. While the chicken is a uricotelic animal, it has measurable OTC activity in its kidney. OTC activity is highly variable within and between chicken breeds. Chicken OTC may have some physiological significance because of its significant activity in the k...

journal_title：Journal of biochemistry

authors： Shimogiri T,Kono M,Mannen H,Mizutani M,Tsuji S

更新日期：1998-11-01 00:00:00

abstract：:Antiserum against Z-protein, which is one of the components of myofibrillar Z-lines, strongly stained the epithelial cells of chicken small intestine at the apical tips of the microvilli and the junctional complexes. Immunoblot tests with anti-Z-protein antiserum showed that there is a peptide of the same antigenicity...

journal_title：Journal of biochemistry

authors： Ohashi K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:1. The endogenous phosphorylation of mouse brain microsomes was studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). 2. It was found that specific proteins and lipids in brain microsomes were phosphorylated by the terminal phosphate of ATP under appropriate con...

journal_title：Journal of biochemistry

authors： Satomi D

更新日期：1977-12-01 00:00:00

abstract：:We studied the regulation of lipid body biogenesis in the oleaginous fungus Mortierella ramanniana var. angulispora by investigating culture conditions to modulate lipid body size, which we found was affected by the carbon-to-nitrogen ratio (C/N ratio) in the culture medium. Increasing the nitrogen source or decreasin...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Noda N,Yamaoka M

更新日期：2004-02-01 00:00:00

abstract：:Spectrophotometric studies revealed the irreversible photodissociation of reduced cytochrome oxidase-nitric oxide (NO) at 5 K. The dissociated NO recombined as the sample temperature was raised, and the half-recombination temperature was 65 K. The photodissociation at 5K was also confirmed by a change in the EPR spect...

journal_title：Journal of biochemistry

authors： Yoshida S,Hori H,Orii Y

更新日期：1980-12-01 00:00:00

abstract：:p-Nitrophenyl O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha- D-glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-alpha-D-glucopyranoside (FG5P) is hydrolyzed by human pancreatic a-amylase (HPA) or salivary alpha-amylase (HSA) to O-6-deoxy-6-[(2-pyridy...

journal_title：Journal of biochemistry

authors： Omichi K,Ikenaka T

更新日期：1986-11-01 00:00:00

abstract：:Partial amino acid sequences of two mitochondrial cytochrome P-450's, P-450 (SCC) and P-450 (11 beta), and two microsomal cytochrome P-450's, P-450 (C-21), and P-450 (17 alpha, Lyase), from adrenal cortex were analyzed and compared. Mitochondrial P-450's and microsomal P-450's were different in the amino acid sequence...

journal_title：Journal of biochemistry

authors： Ogishima T,Okada Y,Kominami S,Takemori S,Omura T

更新日期：1983-11-01 00:00:00

abstract：:Two kinds of myosin phosphatases were purified from fresh chicken gizzard smooth muscle. Alkaline phosphatase (CGP-a), which requires Mg2+, was most active at pH 8.6 with 2 to 4 mM Mg2+, and was essentially the same as the phosphatase we reported previously (J. Biochem. 99, 1027-1036 (1986]. On the other hand, neutral...

journal_title：Journal of biochemistry

authors： Yoshida M,Yagi K

更新日期：1988-02-01 00:00:00

abstract：:The amino acid sequence of glutathione S-transferase a from guinea pig liver was determined. Glutathione S-transferase a was composed of two identical subunits, each comprising 218 amino acid residues. The amino acid sequence of glutathione S-transferase a exhibited 73% homology with that of human glutathione S-transf...

journal_title：Journal of biochemistry

authors： Kamei-Hayashi K,Oshino R,Hara S

更新日期：1993-12-01 00:00:00

abstract：:Ca(2+)/calmodulin-dependent protein kinases (CaM-kinases) I and IV are activated upon phosphorylation of their Thr(177) and Thr(196), respectively, by the upstream Ca(2+)/calmodulin-dependent protein kinases CaM-kinase kinase alpha and beta, and deactivated upon dephosphorylation by protein phosphatases such as CaM-ki...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：2001-10-01 00:00:00

abstract：:Trypsin was used as a probe of F-actin conformation. F-actin is known to be refractory to proteolysis [Jacobson, G.R. and Rosenbusch, J.P. (1976) Proc. Natl. Acad. Sci. U.S. 73, 2742-2746]. However, here it was found that F-actin could also be digested by trypsin to a 33-kDa fragment (like G-actin) when free MgADP is ...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1988-08-01 00:00:00

abstract：:Reports vary on the role of growth hormone (GH) in adipocyte differentiation. In this study, we showed that GH exerted dual effects depending on the stage of differentiation, using a serum-free culture of 3T3-L1 preadipocytes. GH promoted the differentiation when added to the medium during differentiation-inducing tre...

journal_title：Journal of biochemistry

authors： Tominaga S,Morikawa M,Osumi T

更新日期：2002-12-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:Ras is a signal-transducing, guanine nucleotide-binding protein for various membrane receptors including tyrosine kinase receptors. Ras participates in the regulation of cell proliferation, differentiation, and morphology. Activated ras oncogenes have been identified in various forms of human cancer including epitheli...

journal_title：Journal of biochemistry

authors： Yamamoto T,Taya S,Kaibuchi K

更新日期：1999-11-01 00:00:00

abstract：:We have unexpectedly found that Streptomyces subtilisin inhibitor (SSI) and some other similar serine protease inhibitors produced by Streptomycetes strongly inhibit Streptomyces griseus metallo-endopeptidase II (SGMP II) [Kajiwara, K. et al. (1991) J. Biochem. 110, 350-354]. In order to elucidate the mode of their un...

journal_title：Journal of biochemistry

authors： Kumazaki T,Kajiwara K,Kojima S,Miura K,Ishii S

更新日期：1993-10-01 00:00:00

abstract：:Yeast mitochondrial ATP synthase has three regulatory proteins, ATPase inhibitor, 9K protein, and 15K protein. The 9K protein binds directly to purified F1-ATPase, as does the ATPase inhibitor, but the 15K protein does not [Hashimoto, T. et al. (1987) J. Biochem. 102, 685-692]. In the present study, we found that 15K ...

journal_title：Journal of biochemistry

authors： Hashimoto T,Yoshida Y,Tagawa K

更新日期：1990-07-01 00:00:00

abstract：:Previously, several workers reported that at very low ionic strength and in the presence of ATP, the extent of binding of S-1 with the F-actin-tropomyosin-troponin complex or regulated actin (FA-TM-TN) is unaffected by removal of Ca2+. However, in this study we found that during the ATPase reaction at physiological io...

journal_title：Journal of biochemistry

authors： Inoue A,Tonomura Y

更新日期：1982-04-01 00:00:00

abstract：:To study how collagen synthesis is regulated in developing chick embryonic skin, hydroxyproline synthesis, incorporation of proline, and translational activity and content of collagen mRNA in 12-, 15-, and 18-day skins were determined and compared with each other. Hydroxyproline synthesis in the 18-day skins was marke...

journal_title：Journal of biochemistry

authors： Ohyama K,Tokimitsu I,Tajima S

更新日期：1991-08-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:The protein level of the GTS1 gene product (Gts1p) fluctuated during the oscillation of energy metabolism in continuous culture of the yeast Saccharomyces cerevisiae. Here, we found that the GTS1 mRNA level oscillated with the same periodicity as the metabolic oscillation, suggesting that the expression of GTS1 was re...

journal_title：Journal of biochemistry

authors： Tonozuka H,Wang J,Mitsui K,Saito T,Hamada Y,Tsurugi K

更新日期：2001-11-01 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

journal_title：Journal of biochemistry

authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1993-06-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:To establish a system for overproduction of the ferrochelatase [EC 4.99.1.1] from Escherichia coli, a plasmid designated pFC3 was constructed. The 35-kDa protein was accumulated in E. coli DH5 alpha cells that harbored pFC3 to a level equal to approximately 9% of the total protein (roughly 50 mg/liter) upon thermal in...

journal_title：Journal of biochemistry

authors： Miyamoto K,Kanaya S,Morikawa K,Inokuchi H

更新日期：1994-03-01 00:00:00

abstract：:Calpastatin, an endogenous inhibitor protein specifically acting on calpain [EC 3.4.22.17; Ca2+-dependent cysteine proteinase], was purified to apparent homogeneity from the cytosol fraction of human erythrocytes. The yield was 0.38 mg from 400 ml of blood. The purification procedures included DEAE-cellulose and Ultro...

journal_title：Journal of biochemistry

authors： Takano E,Murachi T

更新日期：1982-12-01 00:00:00