Autotaxin--an LPA producing enzyme with diverse functions.

abstract：:In the preceding papers, we showed that one of the two complementar factors of polypeptide chain elongation factor 1 (EF-1) from pig liver, EF-1alpha, functionally corresponds to bacterial EF-Tu (Nagata, S., Iwasaki, K., and Kaziro, Y. (1976) Arch. Biochem. Biophys. 172, 168), while the other, EF-1betagamma, as well a...

journal_title：Journal of biochemistry

authors： Nagata S,Motoyoshi K,Iwasaki K

更新日期：1978-02-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00

abstract：:To reveal the molecular orientation of plastocyanin (PC) on spinach thylakoid membranes, the position of Lys residues modified by acetic anhydride was compared between thylakoid-bound PC and the isolated one. Digestion of the isolated PC by a trypsin yielded a peptide map with seven spots prior to the acetylation of t...

journal_title：Journal of biochemistry

authors： Takano M,Takahashi M,Oobatake M,Asada K

更新日期：1985-11-01 00:00:00

abstract：:CEL-III is a haemolytic lectin, which has two beta-trefoil domains (domains 1 and 2) and a beta-sheet-rich domain (domain 3). In domain 3 (residues 284-432), there is a hydrophobic region containing two alpha-helices (H8 and H9, residues 317-357) and a loop between them, in which alternate hydrophobic residues, especi...

journal_title：Journal of biochemistry

authors： Hisamatsu K,Tsuda N,Goda S,Hatakeyama T

更新日期：2008-01-01 00:00:00

abstract：:Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...

journal_title：Journal of biochemistry

authors： Haraguchi T,Nagano H

更新日期：1983-03-01 00:00:00

abstract：:Soluble guanylate cyclase (sGC) consisting of two different subunits (alpha: Mr = 74,000, beta: Mr = 69,000) was purified more than 12,000-fold in terms of specific activity from the supernatant of bovine lung homogenates and characterized. The heme content determined with the pyridine hemochromogen method and Bradfor...

journal_title：Journal of biochemistry

authors： Tomita T,Tsuyama S,Imai Y,Kitagawa T

更新日期：1997-09-01 00:00:00

abstract：:A new assay method for hydrogenase [EC 1.12.2.1] based on the enzymic electrode reaction of H2-H+ equilibrium has been established. The method is based on the experimental fact that the short-circuit current of the electric cell composed of an electrode with hydrogenase and methylviologen as the mediator of H2-H+ equi...

journal_title：Journal of biochemistry

authors： Yagi T,Goto M,Nakano K,Kimura K,Inokuchi H

更新日期：1975-09-01 00:00:00

abstract：:Thyrotropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the TRH-induced hydrolysis of PIP2, the conversion of phosphatidylinositol 4-phosphate (PIP) to PIP2 and the accumulation...

journal_title：Journal of biochemistry

authors： Suzuki R,Takenawa T

更新日期：1987-09-01 00:00:00

abstract：:In order to study the conformation of the side chain of lysine substrates bound to the active center of trypsin, two lysine analogs, cis- and trans-2,6-diamino-4-hexenoic acids (4,5-dehydrolysines) were synthesized and kinetic parameters for the hydrolysis of benzoyl methyl esters and phenylthiazolones of these analog...

journal_title：Journal of biochemistry

authors： Mizusaki K,Sugahara Y,Tsunematsu H,Makisumi S

更新日期：1986-07-01 00:00:00

abstract：:Incubation of quiescent cultures of Swiss 3T3 cells with epidermal growth factor (EGF) caused an increase in c-myc mRNA. Under these conditions, EGF did not induce phosphoinositide turnover, formation of diacylglycerol, formation of inositol tris-, bis-, and monophosphates, protein kinase C activation, or Ca2+ mobiliz...

journal_title：Journal of biochemistry

authors： Tsuda T,Hamamori Y,Fukumoto Y,Kaibuchi K,Takai Y

更新日期：1986-12-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:A novel positively charged planar bilayer membrane was formed from a mixture containing 20% cationic lipid, 1,2-dioleoyloxy-3-(trimethylammonio)propane, and neutral phospholipid mixture of 56% phosphatidylethanolamine and 24% egg phosphatidylcholine. The basic properties of the bilayer were essentially the same as tho...

journal_title：Journal of biochemistry

authors： Anzai K,Masumi M,Kawasaki K,Kirino Y

更新日期：1993-10-01 00:00:00

abstract：:Enterococcus hirae V-ATPase, in contrast to most V-type ATPases, is resistant to N-ethylmaleimide (NEM). Alignment of the amino acid sequences of NtpA suggests that the NEM-sensitive Cys of V-type ATPases is replaced by Ala in E. hirae V-ATPase. Consistent with this prediction, the V-ATPase became sensitive upon subst...

journal_title：Journal of biochemistry

authors： Murata T,Yoshikawa Y,Hosaka T,Takase K,Kakinuma Y,Yamato I,Kikuchi T

更新日期：2002-11-01 00:00:00

abstract：:Long interspersed nuclear element (LINE) is known to be transposed by reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. Our previous study revealed that the second G residue (G8) in the GGAUA loo...

journal_title：Journal of biochemistry

authors： Otsu M,Kajikawa M,Okada N,Kawai G

更新日期：2017-10-01 00:00:00

abstract：:The peptide bond syntheses catalyzed by subtilisin BPN', papain [EC 3.4.22.2], and pepsin [EC 3.4.23.1] were studied comparatively at the optimum pH of the enzymes with the coupling system Cbz-(AA)n-OH + Leu-X leads to Cbz-(AA)n-Leu-X, in which AA = various amino acid residues, n = 1-3 and X = -NH2, -OEt, -OBut, -ODPM...

journal_title：Journal of biochemistry

authors： Morihara K,Oka T

更新日期：1981-02-01 00:00:00

abstract：:We examined the effect of porcine brain Ca2+-dependent regulator (CDR) protein on microtubule (MT) assembly from microtubular proteins isolated from porcine brain by temperature-dependent cycles of assembly-disassembly. CDR exhibited a potent inhibitory effect on MT assembly in the presence of Ca2+, whereas it had lit...

journal_title：Journal of biochemistry

authors： Nishida E,Kumagai H,Ohtsuki I,Sakai H

更新日期：1979-05-01 00:00:00

abstract：:OsCnfU-1A is a chloroplast-type Nfu-like protein that consists of tandem repeats sharing high sequence homology. Domain I of this protein, but not domain II, has a C-X-X-C motif that is thought to assemble an iron-sulphur cluster. Herein we report the solution structure of OsCnfU-1A domain I (73-153). Although OsCnfU-...

journal_title：Journal of biochemistry

authors： Saio T,Kumeta H,Ogura K,Yokochi M,Asayama M,Katoh S,Katoh E,Teshima K,Inagaki F

更新日期：2007-07-01 00:00:00

abstract：:The flagellar motor is composed of the stator and the rotor, and the interaction between the stator and the rotor at the cytoplasmic region is believed to produce mechanical force for the rotation of flagella. The periplasmic region of the stator has been proposed to play an important role in assembly around and incor...

journal_title：Journal of biochemistry

authors： Hizukuri Y,Kojima S,Homma M

更新日期：2010-09-01 00:00:00

abstract：:Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

journal_title：Journal of biochemistry

authors： Narutaki S,Dunn BM,Oda K

更新日期：1999-01-01 00:00:00

abstract：:Members of the transforming growth factor-β (TGF-β) family, including TGF-βs, activin and bone morphogenetic proteins (BMPs), are multifunctional proteins that regulate a wide variety of cellular responses, such as proliferation, differentiation, migration and apoptosis. TGF-β family signalling is mainly mediated by m...

journal_title：Journal of biochemistry

authors： Imamura T,Oshima Y,Hikita A

更新日期：2013-12-01 00:00:00

abstract：:The reaction of D-amino acid oxidase [EC 1.4.3.3] (DAO) from porcine kidney with beta-cyano-D-alanine (D-BCNA) was studied. DAO was found to catalyze elimination of the cyano group as well as oxidation of D-BCNA. During the course of the reaction in the presence of excess oxygen, an intermediate was observed which exh...

journal_title：Journal of biochemistry

authors： Miura R,Shiga K,Miyake Y,Watari H,Yamano T

更新日期：1980-05-01 00:00:00

abstract：:Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...

journal_title：Journal of biochemistry

authors： Okubo T,Yanai N,Watanabe S,Arai Ki,Obinata M

更新日期：2000-04-01 00:00:00

abstract：:Identification of single-stranded regions in Torulopsis utilis 5S RNA was attempted by the use of Nuclease S1, a single-strand specific endonuclease. When T. utilis 5S RNA was subjected to prolonged incubation with Nuclease S1, about 50% of the substrate 5S RNA remained as large oligonucleotide "cores." Such Nuclease...

journal_title：Journal of biochemistry

authors： Nishikawa K,Takemura S

更新日期：1977-04-01 00:00:00

abstract：:The position of a single N'-formylkynurenine residue in ozone-inactivated hen egg-white lysozyme [EC 3.2.1.17] was determined by two methods. One involved identification of an amino-terminus of the C-peptide obtained by selective cleavage with hydrazine of the kynurenyl peptide linkage in oxidized lysozyme. The other ...

journal_title：Journal of biochemistry

authors： Sakiyama F,Natsuki R

更新日期：1976-01-01 00:00:00

abstract：:We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

journal_title：Journal of biochemistry

authors： Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

更新日期：2008-12-01 00:00:00

abstract：:The cytoplasmic peptide elongation factor, EF-1 alpha, is anchored at the endoplasmic reticulum membrane by phosphatidylinositol via ethanolamine bridging presumably to Asp306 of the protein. ...

journal_title：Journal of biochemistry

authors： Hayashi Y,Urade R,Utsumi S,Kito M

更新日期：1989-10-01 00:00:00

abstract：:Urate oxidases (UOs) catalyze the cofactor-independent oxidation of uric acid, and an extensive water network in the active site has been suggested to play an essential role in the catalysis. For our present analysis of the structure and function of the water network, the crystal qualities of Bacillus sp. TB-90 urate ...

journal_title：Journal of biochemistry

authors： Hibi T,Itoh T

更新日期：2020-10-01 00:00:00

abstract：:Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influence its DNA-binding a...

journal_title：Journal of biochemistry

authors： Xue Y,Wang S,Feng X

更新日期：2009-07-01 00:00:00

abstract：:Hyaluronan (HA), a high-molecular-weight glycosaminoglycan ubiquitously present in the extracellular matrices (ECMs) of animals, plays important roles in ECM organization and cell behavior through binding to hyaluronan-binding proteins (HABPs). We previously reported that HA has anti-inflammatory effects on guinea pig...

journal_title：Journal of biochemistry

authors： Nochi H,Shinomiya T,Tamoto K

更新日期：2006-01-01 00:00:00

abstract：:A high molecular weight protease inhibitor was purified from the egg white of Cuban crocodile (Crocodylus rhombifer). It inhibited the casein hydrolyzing activity of trypsin, subtilisin and papain. Its native molecular weight was 730,000 and it consisted of four subunits of equal molecular weight, each pair of which w...

journal_title：Journal of biochemistry

authors： Ikai A,Kitamoto T,Nishigai M

更新日期：1983-01-01 00:00:00