Abstract:
:The position of a single N'-formylkynurenine residue in ozone-inactivated hen egg-white lysozyme [EC 3.2.1.17] was determined by two methods. One involved identification of an amino-terminus of the C-peptide obtained by selective cleavage with hydrazine of the kynurenyl peptide linkage in oxidized lysozyme. The other was to analyze a tryptic peptide containing kynurenine in the modified enzyme. Both methods showed that tryptophan 62 in lysozyme was so sensitive to ozone as to be selectively oxidized to N'-formylkynurenine with concomitant loss of the lytic activity.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Sakiyama F,Natsuki Rdoi
10.1093/oxfordjournals.jbchem.a131051subject
Has Abstractpub_date
1976-01-01 00:00:00pages
225-8issue
1eissn
0021-924Xissn
1756-2651journal_volume
79pub_type
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