Abstract:
:In order to study the conformation of the side chain of lysine substrates bound to the active center of trypsin, two lysine analogs, cis- and trans-2,6-diamino-4-hexenoic acids (4,5-dehydrolysines) were synthesized and kinetic parameters for the hydrolysis of benzoyl methyl esters and phenylthiazolones of these analogs by this enzyme were compared with those of the corresponding lysine derivatives. The derivatives of cis-4,5-dehydrolysine were hydrolyzed much more slowly than those of lysine, owing largely to the small kcat values for the former. On the other hand, the derivatives of the trans-isomer were hydrolyzed at about the same rates as those of lysine and the values of both Km and kcat of the former are also similar to those of the latter. These results indicate that the conformation of the side chain of the lysine derivatives hydrolyzed by trypsin is such that the beta- and epsilon-carbons are in a trans-like conformation, as suggested by X-ray crystallographic studies of inhibitor-trypsin complex.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Mizusaki K,Sugahara Y,Tsunematsu H,Makisumi Sdoi
10.1093/oxfordjournals.jbchem.a121695subject
Has Abstractpub_date
1986-07-01 00:00:00pages
21-5issue
1eissn
0021-924Xissn
1756-2651journal_volume
100pub_type
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