Semi-micro-scale frontal gel chromatography of interacting systems of a protein and small molecules: binding of warfarin, tryptophan, or FMN to albumin, and of o-nitrophenol to catechol 2,3-dioxygenase.

abstract：:NADP+-specific isocitrate dehydrogenase was found in several tissues of the pupa of the silkworm, Bombyx mori. This enzyme was highly purified from the whole bodies of pupae. This is the first isolation of the enzyme from insect materials. The purified enzyme gave a single protein band on polyacrylamide gel electropho...

journal_title：Journal of biochemistry

authors： Miake F,Torikata T,Koga K,Hayashi K

更新日期：1977-08-01 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:In a previous report (J. Biochem. 89, 1333-1335, 1981) we showed that 30 degrees C-treatment (pCa less than 6 and 2 mM MgCl2), like EDTA-treatment, caused a reversible removal of regulatory light-chains from Akazara adductor myosin. Utilizing the heat-treatment, we now show (a) that not half but the total removal of r...

journal_title：Journal of biochemistry

authors： Ojima T,Nishita K,Watanabe S

更新日期：1983-02-01 00:00:00

abstract：:A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and r...

journal_title：Journal of biochemistry

authors： Tsugita A,Uchida T,Mewes HW,Ataka T

更新日期：1987-12-01 00:00:00

abstract：:Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...

journal_title：Journal of biochemistry

authors： Haraguchi T,Nagano H

更新日期：1983-03-01 00:00:00

abstract：:We studied the mechanism of formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study. The hydroxyl radical is also generated through Fenton t...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Tero-Kubota S,Yamamoto Y,Kato M,Nakano M,Yagi K,Ogino K

更新日期：2003-12-01 00:00:00

abstract：:ZFP36L1 is an RNA-binding protein responsible for mRNA decay in the cytoplasm. ZFP36L1 has also been suggested as a nuclear-cytoplasmic shuttling protein because it contains a potential nuclear localization signal and a nuclear export signal. However, it remains unclear how the nuclear localization of ZFP36L1 is contr...

journal_title：Journal of biochemistry

authors： Matsuura Y,Noguchi A,Sakai S,Yokota N,Kawahara H

更新日期：2020-11-01 00:00:00

abstract：:We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion fragments of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universa...

journal_title：Journal of biochemistry

authors： Kang DC,Takeshige K,Minakami S

更新日期：1990-12-01 00:00:00

abstract：:Human platelet membrane glycoproteins IIb (GPIIb) and IIIa (GPIIIa), which have been proposed to be subunits of a receptor for fibrinogen, were purified from Triton X-100-solubilized platelet membranes by affinity chromatography on a concanavalin A (Con A)-Sepharose column followed by preparative sodium dodecyl sulfat...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-11-01 00:00:00

abstract：:The interaction between riboflavin and riboflavin binding protein (RBP) was studied by 13C-NMR spectroscopy. The 13C-NMR spectra of riboflavin selectively enriched at the 2-, 4-, 4a-, and 10a-positions and of (3-[13C]methyl)riboflavin were measured both in the free and RBP-bound forms. The 13C signals of 13C-enriched ...

journal_title：Journal of biochemistry

authors： Miura R,Tojo H,Fujii S,Yamano T,Miyake Y

更新日期：1984-07-01 00:00:00

abstract：:An escape mutant of human parainfluenza virus type 1 (hPIV1), which was selected by serial passage in the presence of a sialidase inhibitor, 4-O-thiocarbamoylmethyl-2-deoxy-2,3-didehydro-N-acetylneur-aminic acid (TCM-Neu5Ac2en), exhibited remarkable syncytium formation and virus-induced cell death in LLC-MK2 cells but...

journal_title：Journal of biochemistry

authors： Takaguchi M,Takahashi T,Hosokawa C,Ueyama H,Fukushima K,Hayakawa T,Itoh K,Ikeda K,Suzuki T

更新日期：2011-02-01 00:00:00

abstract：:A high molecular weight protease inhibitor was purified from the egg white of Cuban crocodile (Crocodylus rhombifer). It inhibited the casein hydrolyzing activity of trypsin, subtilisin and papain. Its native molecular weight was 730,000 and it consisted of four subunits of equal molecular weight, each pair of which w...

journal_title：Journal of biochemistry

authors： Ikai A,Kitamoto T,Nishigai M

更新日期：1983-01-01 00:00:00

abstract：:A fluorescent fragment of Mr = 23,800 was obtained by the papain digestion of N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene diamine (abbreviated as IAEDANS)-modified chicken gizzard myosin. The fragment was isolated by gel filtration on a Sephadex G-100 column in the presence of 5 M guanidine-HCl followed by anion exch...

journal_title：Journal of biochemistry

authors： Onishi H,Maita T,Miyanishi T,Watanabe S,Matsuda G

更新日期：1986-12-01 00:00:00

abstract：:The midpoint pCa value of the fluorescence titration curve of the complex of 2-[4'-iodoacetamido)anilino)-naphthalene-6-sulfonic acid-labeled troponin (IAANS-Tn) and nonpolymerizable tropomyosin (NPTm) was much larger than that for the complex of Tn containing dansylaziridine-labeled troponin C (DANZ-TnC) and NPTm. Th...

journal_title：Journal of biochemistry

authors： Iio T,Nishio T,Kondo H

更新日期：1988-08-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:ADAMTS13 is the metalloprotease responsible for the proteolytic degradation of von Willebrand factor (VWF). A severe deficiency of this VWF-cleaving protease activity causes thrombotic thrombocytopenic purpura. This protease, comprising 1,427 amino acid residues, is composed of multiple domains, i.e., a preproregion, ...

journal_title：Journal of biochemistry

authors： Soejima K,Nakamura H,Hirashima M,Morikawa W,Nozaki C,Nakagaki T

更新日期：2006-01-01 00:00:00

abstract：:The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...

journal_title：Journal of biochemistry

authors： Miyanishi T,Toyoshima C,Wakabayashi T,Matsuda G

更新日期：1988-03-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a class of bioactive lipid molecules in animal tissues, including the endocannabinoid anandamide and the anti-inflammatory substance N-palmitoylethanolamine. Enzymatic hydrolysis of NAEs is considered to be an important step to regulate their endogenous levels. Lysosomal NAE-hydrolysing ...

journal_title：Journal of biochemistry

authors： Wang J,Zhao LY,Uyama T,Tsuboi K,Wu XX,Kakehi Y,Ueda N

更新日期：2008-11-01 00:00:00

abstract：:Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-H...

journal_title：Journal of biochemistry

authors： Takabe T,Niwa S,Ishikawa H,Miyakawa M

更新日期：1980-01-01 00:00:00

abstract：:The sequence of the 5'-terminal 106 nucleotides of cucumber mosaic virus (strain Y) RNA 4, the mRNA coding for viral coat protein, has been determined. The first AUG was located at 77 nucleotides from the 5'-terminus and was confirmed to be an initiation codon by analysis of the N-terminal amino acid sequence of the p...

journal_title：Journal of biochemistry

authors： Hidaka S,Tsunasawa S,Yoon J,Narita K,Takanami Y,Kubo S,Miura K

更新日期：1985-01-01 00:00:00

abstract：:Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsi...

journal_title：Journal of biochemistry

authors： Matsuda T,Watanabe K,Nakamura R

更新日期：1983-07-01 00:00:00

abstract：:Guanosine 3',5'-monophosphate (cyclic GMP)-dependent protein kinase purified from silkworm pupae reacts with rat liver ribosomal proteins when a stimulatory modulator (Kuo, W.N. & Kuo, J.F. 1976) J. Biol. Chem. 251, 4283-4286) is added to the reaction mixture. Judging from autoradiogram of the radioactive proteins sep...

journal_title：Journal of biochemistry

authors： Chihara-Nakashima M,Hashimoto E,Nishizuka Y

更新日期：1977-06-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:A synchronous enzyme-reaction system using water-soluble formazan and a non-enzymatic electron mediator was developed and applied to an enzyme immunoassay (EIA). The reaction system consists of four steps: (I) dephosphorylation of NADP(+) to produce NAD(+) by alkaline phosphatase (ALP), (II) reduction of NAD(+) to pro...

journal_title：Journal of biochemistry

authors： Inouye K,Ueno I,Yokoyama S,Sakaki T

更新日期：2002-01-01 00:00:00

abstract：:alpha-Actinin purified from chicken gizzard smooth muscle was characterized in comparison with alpha-actinins from chicken striated muscles, or fast-skeletal muscle, slow-skeletal muscle, and cardiac muscle. The gizzard alpha-actinin molecule consisted of two apparently identical subunits with a molecular weight of 10...

journal_title：Journal of biochemistry

authors： Endo T,Masaki T

更新日期：1982-11-01 00:00:00

abstract：:Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

journal_title：Journal of biochemistry

authors： Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

更新日期：2001-04-01 00:00:00

abstract：:The oleate (delta 9-18:1)/cis-vaccenate (delta 11-18:1) ratios in phospholipids increased in the order of normal liver, host liver and hepatoma in rats. The amount of oleate increased in phospholipids and decreased in triacylglycerol in the same order, whereas the distributions of cis-vaccenate among the major lipid c...

journal_title：Journal of biochemistry

authors： Hori T,Okuyama H

更新日期：1987-05-01 00:00:00

abstract：:OS-9 gene is frequently coamplified with CDK4 gene in human sarcomas. We isolated and characterized three isoforms of OS-9 cDNA found in a myeloid leukemia HL-60 cDNA library. Isoform 1 consisted of 2,700 bp, from which a 667 amino acid sequence was deduced and found to be identical with that of OS-9 cDNA from osteosa...

journal_title：Journal of biochemistry

authors： Kimura Y,Nakazawa M,Yamada M

更新日期：1998-05-01 00:00:00

abstract：:An in vitro system for accurate transcription initiation by RNA polymerase II was developed using Ehrlich ascites tumor cells. Truncated DNA containing adenovirus 2 major late promoter was faithfully transcribed in this lysate, although the efficiency of transcription was lower than that in a HeLa cell lysate. Creatin...

journal_title：Journal of biochemistry

authors： Ohtsuki M,Sekimizu K,Natori S

更新日期：1984-10-01 00:00:00