The degradation rates of type I, II, and III collagens by tadpole collagenase.

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

journal_title：Journal of biochemistry

authors： Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

更新日期：1982-06-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

journal_title：Journal of biochemistry

authors： Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

更新日期：2000-07-01 00:00:00

abstract：:1. The addition of palmitic acid or myristic acid to a medium containing delipidated fetal bovine serum resulted in severe inhibition of V79-R cell growth. The degree of inhibition by palmitic acid was concentration dependent. Simultaneous addition of oleic acid protected the cells from the inhibition by palmitic acid...

journal_title：Journal of biochemistry

authors： Urade R,Kito M

更新日期：1982-05-01 00:00:00

abstract：:We designed mutant lysozymes with N-glycosylation signal sequences (Asn48-Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkholder minimum medium, N-glycosylation occurred in both lysozymes....

journal_title：Journal of biochemistry

authors： Ueda T,Iwashita H,Hashimoto Y,Imoto T

更新日期：1996-01-01 00:00:00

abstract：:Rho family GTPases act as transducers of signals from extracellular stimuli to the cytoskeleton and gene expression. Their actions are temporal and spatial determinants for cellular functions. The cellular functions of Rho family GTPases have been studied in fibroblasts and endothelial cells, and recent advances have ...

journal_title：Journal of biochemistry

authors： Negishi M,Katoh H

更新日期：2002-08-01 00:00:00

abstract：:In order to study the conformation of the side chain of lysine substrates bound to the active center of trypsin, two lysine analogs, cis- and trans-2,6-diamino-4-hexenoic acids (4,5-dehydrolysines) were synthesized and kinetic parameters for the hydrolysis of benzoyl methyl esters and phenylthiazolones of these analog...

journal_title：Journal of biochemistry

authors： Mizusaki K,Sugahara Y,Tsunematsu H,Makisumi S

更新日期：1986-07-01 00:00:00

abstract：:Protein carbonyls are reported to increase in aging and in pathologies such as Alzheimer's disease and ischemic injury. Detailed study of this important issue has, however, been hampered by lack of an appropriate method to identify individual carbonylated proteins. We describe here an immunoblot method to identify ind...

journal_title：Journal of biochemistry

authors： Nakamura A,Goto S

更新日期：1996-04-01 00:00:00

abstract：:YfkN isolated from the culture supernatant of Bacillus subtilis in the exponential phase of growth is a protein of 143.5 kDa that derives from a putative large precursor of 159.6 kDa processed at both the N- and C-terminal ends. Pulse-chase experiments indicated that the release occurs slowly with a half-time longer t...

journal_title：Journal of biochemistry

authors： Chambert R,Pereira Y,Petit-Glatron MF

更新日期：2003-11-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:Wild mice are divided into two groups with different ganglioside compositions in the liver. Most Japanese and a few Chinese wild mice have GM2(NeuGc) as a major ganglioside, whereas all wild mice caught at other places distributed all over the world other than Japan and China express GM1(NeuGc) and GD1a(NeuGc) in addi...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki A,Yamakawa T,Wang CH,Bonhomme F,Miyashita N,Moriwaki K

更新日期：1984-01-01 00:00:00

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...

journal_title：Journal of biochemistry

authors： Hamidi S,Sheng G

更新日期：2018-10-01 00:00:00

abstract：:Streptolysin S (SLS) is a serum-extractable and oxygen-stable hemolysin produced by Group A Streptococcus. A SLS-deficient mutant in which transposon Tn 916 was inserted in a locus distinct from the sag gene cluster [Nizet et al. (2000) Infect. Immun. 68, 4245-4254] was obtained by filter mating of the transposon-harb...

journal_title：Journal of biochemistry

authors： Karaya K,Shimizu T,Taketo A

更新日期：2001-05-01 00:00:00

abstract：:ISG15 is a ubiquitin-like protein that is upregulated on treatment with interferon. ISG15 is considered to be covalently conjugated to cellular proteins through a sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1...

journal_title：Journal of biochemistry

authors： Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

更新日期：2005-12-01 00:00:00

abstract：:A completely sodium dodecyl sulfate-insoluble protein was obtained from plasmodia of Physarum polycephalum. The amino acid composition of this protein was very similar to that of connectin, an elastic protein of muscle. When the protein suspension was treated with 6 M guanidine-HCl in the presence of 0.1 M 2-mercaptoe...

journal_title：Journal of biochemistry

authors： Ozaki K,Maruyama K

更新日期：1980-09-01 00:00:00

abstract：:Isolation and characterization of a fibrous protein component which might be associated with contractile ring of a dividing Tetrahymena cell were attempted by making use of coprecipitation of the protein with rabbit skeletal muscle myosin. The protein was purified to homogeneity by ammonium sulfate fractionation betwe...

journal_title：Journal of biochemistry

authors： Numata O,Yasuda T,Hirabayashi T,Watanabe Y

更新日期：1980-11-01 00:00:00

abstract：:HUT-14 cells, tumorigenic human fibroblasts, express a mutant beta-actin which has a single amino acid substitution at position 244 (glycine to aspartic acid), in addition to normal beta- and gamma-actin. In order to characterize the biochemical function of the mutant beta-actin, actins were extracted and purified fro...

journal_title：Journal of biochemistry

authors： Taniguchi S,Sagara J,Kakunaga T

更新日期：1988-04-01 00:00:00

abstract：:Tryptophan residues in ribonuclease from a Rhizopus sp. (RNase Rh) were modified by NBS, H2O2-dioxane, o-nitrophenylsulfenyl chloride (NPS-Cl) and the relation between the extent of modification and enzymatic activity was studied in each case. By extrapolation of the modified tryptophan residue-enzymatic activity curv...

journal_title：Journal of biochemistry

authors： Sanda A,Irie M

更新日期：1980-04-01 00:00:00

abstract：:The structural glycoprotein E(rns) of classical swine fever virus (CSFV) is one of the major antibody targets upon infection of pigs with the virus. Molecular dissection of the structure of E(rns) would define the minimal immunodominant regions that induce antibody responses after infection and may thus help design an...

journal_title：Journal of biochemistry

authors： Lin M,Trottier E,Pasick J,Sabara M

更新日期：2004-12-01 00:00:00

abstract：:Gp91(phox) is a key component of the phagocyte NADPH oxidase. Mutations of its promoter found in patients with chronic granulomatous disease cause deficient binding of PU.1 and HAF-1. Because the two factors bind to the same site (Pu box) of the promoter, we attempted to clarify their relative in vivo contributions to...

journal_title：Journal of biochemistry

authors： Islam MR,Fan C,Fujii Y,Hao LJ,Suzuki S,Kumatori A,Yang D,Rusvai E,Suzuki N,Kikuchi H,Nakamura M

更新日期：2002-04-01 00:00:00

abstract：:The active site of thermolysin is composed of one zinc ion and five polypeptide regions [N-terminal sheet (Asn112-Trp115), alpha-helix 1 (Val139-Thr149), C-terminal loop 1 (Asp150-Gly162), alpha-helix 2 (Ala163-Val176) and C-terminal loop 2 (Gln225-Ser234)]. To explore their catalytic roles, we introduced single amino...

journal_title：Journal of biochemistry

authors： Kusano M,Yasukawa K,Inouye K

更新日期：2009-01-01 00:00:00

abstract：:Microbacterium sp. M874 produced a glyceroglycolipid, di-O-12-methyl-tetradecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol, at about the 50 microM level. Though the strain was highly resistant to tertiary-butyl hydroperoxide (tBHP) in a glycolipid-productive medium, the resistance was reduced in a nonproductive medium...

journal_title：Journal of biochemistry

authors： Nakata K

更新日期：2000-05-01 00:00:00

abstract：:The conformation of the chondroitin-4-sulfate calcium salt was investigated by X-ray analysis. The following results were obtained. 1, The repeat length per disaccharide was 0.913 nm: 2, The molecular chain had three-fold screw symmetry: 3, The shape of the unit cell was a trigonal prism with dimensions a=b=1.28 nm, c...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-01-01 00:00:00

abstract：:The Serratia marcescens serine protease gene encoding a 1,045-amino-acid precursor protein of 112 kDa directs excretion of the mature protease of ca. 58 kDa through the outer membrane of Escherichia coli. A typical signal peptide of 27 amino acids and a large COOH-terminal domain of the precursor are both functionally...

journal_title：Journal of biochemistry

authors： Shikata S,Shimada K,Kataoka H,Horinouchi S,Beppu T

更新日期：1992-05-01 00:00:00

abstract：:1. A major glucoamylase [EC 3.2.1.3] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

journal_title：Journal of biochemistry

authors： Takahashi T,Inokuchi N,Irie M

更新日期：1981-01-01 00:00:00

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:The phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme catalyzes reversibly the intra-molecular phosphoryl interconverting reaction of mannose-6-phosphate and mannose-1-phosphate or glucose-6-phosphate and glucose-1-phosphate. Glucose-6-phosphate and glucose-1-phosphate are known to be utilized for energy metaboli...

journal_title：Journal of biochemistry

authors： Akutsu J,Zhang Z,Tsujimura M,Sasaki M,Yohda M,Kawarabayasi Y

更新日期：2005-08-01 00:00:00

abstract：:D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we show that although these Cdks phosphorylate multiple residues i...

journal_title：Journal of biochemistry

authors： Takaki T,Fukasawa K,Suzuki-Takahashi I,Semba K,Kitagawa M,Taya Y,Hirai H

更新日期：2005-03-01 00:00:00