Chain elongation of fusaric acid and related compounds in rat liver.

abstract：:When human erythrocytes subjected to a pressure of 2.0 kbar were incubated at 0 degree C and atmospheric pressure, hemoglobin was released from the membrane. Effects of drugs, salts, and phospholipid vesicles on hemoglobin release were examined. The hemoglobin release was suppressed by the addition of amphiphathic dru...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Kuranoshita K,Harano T,Kimoto E

更新日期：1993-04-01 00:00:00

abstract：:The spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, had a complex spectrum of neutral glycosphingolipids. CDS, CTS, CTeS were isolated as minor components (together they accounted for 3% of the total neutral glycolipid fraction), while CMS and the glycolipids (Lipid I, II, and III) with larger saccharide...

journal_title：Journal of biochemistry

authors： Sugita M,Yamamoto T,Masuda S,Itasaka O,Hori T

更新日期：1981-11-01 00:00:00

abstract：:The effect of extracellular type-II phospholipase A2 (PLA2) on prostaglandin (PG) synthesis has been studied using rat liver-derived BRL-3A cells. The addition of type-II PLA2 to the medium of BRL-3A cells resulted in a marked decrease in the enzymatic activity in the medium. An immunochemical study involving an anti-...

journal_title：Journal of biochemistry

authors： Suga H,Murakami M,Kudo I,Inoue K

更新日期：1995-11-01 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:HPC-1/syntaxin 1A (HPC-1), which plays an important role in vesicular transport to the plasma membrane, possesses a hydrophobic sequence at its C terminus. When expressed from cDNA in COS cells, wild-type HPC-1 was localized in the Golgi complex and the plasma membrane. Truncation of the hydrophobic domain resulted in...

journal_title：Journal of biochemistry

authors： Masaki R,Yamamoto A,Akagawa K,Tashiro Y

更新日期：1998-08-01 00:00:00

abstract：:Phase transition was detected by a fluorescence polarization technique in the membrane of Thermus thermophilus HB8: it was found to be a function of cell growth temperature when the cell growth temperature was varied between 50-80 degrees C. A systematic relation between the phase transition temperature and the growth...

journal_title：Journal of biochemistry

authors： Wakayama N,Oshima T

更新日期：1978-06-01 00:00:00

abstract：:Laccase is an enzyme that catalyzes the oxidation of phenolic compounds by coupling the reduction of oxygen to water. While many laccases have been identified in plant and fungal species, enzymes of prokaryotic origin are poorly known. Here we report the enzymological characterization of EpoA, a laccase-like extracyto...

journal_title：Journal of biochemistry

authors： Endo K,Hayashi Y,Hibi T,Hosono K,Beppu T,Ueda K

更新日期：2003-05-01 00:00:00

abstract：:The organization of troponin I (TnI) genes from the ascidian Halocynthia roretzi have been determined. Halocynthia possesses roughly two types of TnI isoforms. One type is a single-copied adult TnI (adTnI) gene, which contains eight exons and seven introns. adTnI expresses two isoforms, the shorter body wall muscle Tn...

journal_title：Journal of biochemistry

authors： Yuasa HJ,Kawamura K,Yamamoto H,Takagi T

更新日期：2002-07-01 00:00:00

abstract：:The interactions of porcine alpha2-macroglobulin (alpha2M) with native proteinases, their zymogens and the chemically-modified enzymes were compared. The alpha2M did not bind to chymotrypsinogen, or to most of the chemically modified derivatives of alpha-chymotrypsin, trypsinogen, DIP- and PMS-trypsins, but it could i...

journal_title：Journal of biochemistry

authors： Tsuru D,Kado K,Fujiwara K,Tmimatsu M,Ogita K

更新日期：1978-05-01 00:00:00

abstract：:We sought to evaluate the mechanism(s) associated with pro matrix metalloprotease 2 (proMMP-2) activation in bovine pulmonary artery smooth muscle cells. Preincubation of cells with anti-TNFR1 antibody prevented tumour necrosis factor-α (TNF-α)-induced proMMP-2 activation and increase in membrane type 1 matrix metallo...

journal_title：Journal of biochemistry

authors： Roy S,Chakraborti T,Chowdhury A,Chakraborti S

更新日期：2013-03-01 00:00:00

abstract：:The nucleotide sequence of IStaqTZ2 are available in the DDBJ/EMBL/GenBank databases under the accession number AB063392. A novel insertion sequence (IStaqTZ2) was transposed from the genome of Thermus thermophilus TZ2 to that of the thermophilic bacteriophage IN93. The complete nucleotide sequence of IStaqTZ2 was det...

journal_title：Journal of biochemistry

authors： Matsushita I,Yanase H

更新日期：2009-12-01 00:00:00

abstract：:1. The light-induced pH change of chromatophore suspensions from Rhodospirillum rubrum was stimulated significantly and similarly by KCl, NaCl, LiCl, RbCl, CsCl, MgCl2, MnCl2, and CaCl2. In the dark, the pH of chromatophore suspensions decreased immediately and markedly on adding these salts. 2. The light-induced pH c...

journal_title：Journal of biochemistry

authors： Nishi N,Sakata-Sogawa K,Soe G,Yamashita J

更新日期：1977-11-01 00:00:00

abstract：:To establish a system for overproduction of the ferrochelatase [EC 4.99.1.1] from Escherichia coli, a plasmid designated pFC3 was constructed. The 35-kDa protein was accumulated in E. coli DH5 alpha cells that harbored pFC3 to a level equal to approximately 9% of the total protein (roughly 50 mg/liter) upon thermal in...

journal_title：Journal of biochemistry

authors： Miyamoto K,Kanaya S,Morikawa K,Inokuchi H

更新日期：1994-03-01 00:00:00

abstract：:To investigate the function of the gamma-carboxyglutamic acid (Gla) residues of factor IXa in the activation of factor X, a new species of bovine factor IXa, designated "factor IXa beta'," and its corresponding Gla-domainless form, designated "Gla-domainless factor IXa beta'," were prepared under controlled conditions...

journal_title：Journal of biochemistry

authors： Morita T,Fukudome K,Miyata T,Iwanaga S

更新日期：1991-12-01 00:00:00

abstract：:Vasoactive intestinal peptide (VIP), a neuropeptide coupled with adenylate cyclase, was found to induce neurite extension of PC12h cells. Neurites appeared within 1 h after addition of VIP and extended for at least 24 h. The half-maximal concentration for the effect of VIP was 50 nM. In addition to the morphological c...

journal_title：Journal of biochemistry

authors： Okumura N,Miyatake Y,Takao T,Tamaru T,Nagai K,Okada M,Nakagawa H

更新日期：1994-02-01 00:00:00

abstract：:The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification. The purified preparation of the reductase showed an apparent molecular weight of 27,000 daltons. The reductase appeared to contain loosely-bound FAD as a prosthetic gr...

journal_title：Journal of biochemistry

authors： Kubota S,Yoshida Y,Kumaoka H

更新日期：1977-01-01 00:00:00

abstract：:Urate oxidases (UOs) catalyze the cofactor-independent oxidation of uric acid, and an extensive water network in the active site has been suggested to play an essential role in the catalysis. For our present analysis of the structure and function of the water network, the crystal qualities of Bacillus sp. TB-90 urate ...

journal_title：Journal of biochemistry

authors： Hibi T,Itoh T

更新日期：2020-10-01 00:00:00

abstract：:A simple, sensitive, and accurate method for determination of cholestanol in plasma is described. In this method a fixed amount of cholestane is added to 1 ml of plasma as an internal standard and steroids are saponified and extracted with n-hexane. The amounts of cholestanol and cholesterol are determined by mass fra...

journal_title：Journal of biochemistry

authors： Seyama Y,Ichikawa K,Yamakawa T

更新日期：1976-08-01 00:00:00

abstract：:Saccharomyces cerevisiae has three distinct citrate synthases, two located in mitochondria (mature Cit1p and Cit3p) and one in peroxisomes (mature Cit2p). While the precursor of the major mitochondrial enzyme, Cit1p, has a signal for mitochondrial targeting at its N-terminus (MTS), Cit2p has one for peroxisomal target...

journal_title：Journal of biochemistry

authors： Lee JG,Cho SP,Lee HS,Lee CH,Bae KS,Maeng PJ

更新日期：2000-12-01 00:00:00

abstract：:The interaction between n-octyl-beta-D-glucopyranoside (octyl glucoside) and bovine liver glutamate dehydrogenase (GDH) was studied using techniques including equilibrium dialysis, UV-spectrophotometry, circular dichroism (CD), fluorescence energy transfer and extrinsic spectrofluorometry in 50 mM sodium phosphate buf...

journal_title：Journal of biochemistry

authors： Ghobadi S,Safarian S,Moosavi-Movahedi AA,Ranjbar B

更新日期：2001-11-01 00:00:00

abstract：:The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

journal_title：Journal of biochemistry

authors： Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

更新日期：2008-10-01 00:00:00

abstract：:It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynam...

journal_title：Journal of biochemistry

authors： Liu J,Song Y,Tian B,Qian J,Dong Y,Liu J,Liu B,Sun Z

更新日期：2010-12-01 00:00:00

abstract：:Both rabbit liver microsomes and reconstituted system with purified cytochrome P-450 and cofactors enzymatically oxidized o-dithiane (1, 2-dithiane), 3-methyl-o-dithiane, thiane and 2-methylthiane to the corresponding mono-oxygenated products; sulfides or disulfides were oxidized to the corresponding sulfoxides or thi...

journal_title：Journal of biochemistry

authors： Fukushima D,Kim YH,Iyanagi T,Oae S

更新日期：1978-04-01 00:00:00

abstract：:3-Ketovalidoxylamine A C-N lyase was purified about 900-fold from the cell-free extract of Flavobacterium saccharophilum by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate (SDS) polyacry...

journal_title：Journal of biochemistry

authors： Takeuchi M,Asano N,Kameda Y,Matsui K

更新日期：1985-12-01 00:00:00

abstract：:To obtain direct evidence for the attachment of 5SrRNA-ribosomal L5 protein particles (5SRNP) and methionine-tRNA (tRNA(met)) to methionyl-tRNA synthetase (MetRS) in the macromolecular aminoacyl-tRNA synthetase (ARS) complex of rat liver, a MetRS-5SRNP-tRNA(met) complex was dissociated from the macromolecular ARS comp...

journal_title：Journal of biochemistry

authors： Ogata K,Ohno R,Morioka S,Terao K

更新日期：1996-11-01 00:00:00

abstract：:It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...

journal_title：Journal of biochemistry

authors： Yamato K,Handa S,Yamakawa T

更新日期：1975-12-01 00:00:00

abstract：:1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

journal_title：Journal of biochemistry

authors： Kohama K

更新日期：1979-09-01 00:00:00

abstract：:The technique of saturation transfer electron spin resonance has been applied to study the rotational diffusion of spin-labeled Ca2+, Mg2+-dependent ATPase molecules in the membranes of sarcoplasmic reticulum vesicles. Comparison of the present data with those for spin-labeled hemoglobin undergoing isotropic rotation ...

journal_title：Journal of biochemistry

authors： Kirino Y,Ohkuma T,Shimizu H

更新日期：1978-07-01 00:00:00

abstract：:The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...

journal_title：Journal of biochemistry

authors： Kikuchi K,Umehara T,Fukuda K,Hwang J,Kuno A,Hasegawa T,Nishikawa S

更新日期：2003-03-01 00:00:00

abstract：:Crystals as large as 0.5 X 0.3 X 0.2 mm of purified arginine-transfer RNA from Escherichia coli have been prepared by a vapour diffusion method. X-ray diffraction photographs showed that the crystals gave reflections up to 3.7 A spacing. They have a trigonal space group P31 2 1 (OR P32 2 1) and cell-dimensions a=97.2,...

journal_title：Journal of biochemistry

authors： Morikawa K,Sakamaki T,Nishimura Y,Mitsui Y,Aoki K,Iitaka Y,Tsuboi M,Nishimura S

更新日期：1978-08-01 00:00:00