Saturation transfer electron spin resonance study on the rotational diffusion of calcium- and magnesium-dependent adenosine triphosphatase in sarcoplasmic reticulum membranes.

abstract：:We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. Th...

journal_title：Journal of biochemistry

authors： Ema M,Matsushita N,Sogawa K,Ariyama T,Inazawa J,Nemoto T,Ota M,Oshimura M,Fujii-Kuriyama Y

更新日期：1994-10-01 00:00:00

abstract：:A dinuclear La3+ complex hydrolyzes the phosphodiester bond in diribonucleotides efficiently under mild conditions. The rate of hydrolysis is remarkably accelerated on the complex formation between La3+ and TPHP (TPHP = N,N,N',N'-tetrakis [(2-pyridyl)methyl]-2-hydroxy-1,3-diaminopropane), and the highest activity is a...

journal_title：Journal of biochemistry

authors： Yashiro M,Ishikubo A,Komiyama M

更新日期：1996-12-01 00:00:00

abstract：:We tested the capsid targeted viral inactivation method as an anti-HBV strategy. HepG2 cells were cotransfected with HBV expression plasmid and the plasmid encoding fusion protein of either Core-A3C or Core-humanized renilla GFP (hrGFP). Core-A3C had substantial effect on HBV DNA levels. In the HepG2 cells expressing ...

journal_title：Journal of biochemistry

authors： Li D,Liu J,Kang F,Guan W,Gao X,Wang Y,Sun D

更新日期：2011-10-01 00:00:00

abstract：:Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

journal_title：Journal of biochemistry

authors： Yamashita T,Kawakita M

更新日期：1992-02-01 00:00:00

abstract：:We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

journal_title：Journal of biochemistry

authors： Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

更新日期：2011-09-01 00:00:00

abstract：:Aldehyde reductases from several mammalian and avian tissues transferred the pro-4R hydrogen of NADPH to the substrate, whereas the stereospecificity of carbonyl reductases was not uniform being correlated with the ability to catalyze the oxidoreduction of hydroxysteroids. ...

journal_title：Journal of biochemistry

authors： Nakayama T,Hara A,Kariya K,Sawada H

更新日期：1985-10-01 00:00:00

abstract：:Rat platelets released phospholipase A2 and lysophospholipase upon activation with thrombin or ADP. The release of phospholipases was energy-dependent and was not in parallel with that of a known lysosomal marker enzyme, N-acetyl-beta-D-glucosaminidase. The phospholipases are derived from other granules (dense granule...

journal_title：Journal of biochemistry

authors： Horigome K,Hayakawa M,Inoue K,Nojima S

更新日期：1987-01-01 00:00:00

abstract：:A DNA polymerase beta (pol. beta) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-beta-D-glucoside, prunasin. The compound inhib...

journal_title：Journal of biochemistry

authors： Mizushina Y,Takahashi N,Ogawa A,Tsurugaya K,Koshino H,Takemura M,Yoshida S,Matsukage A,Sugawara F,Sakaguchi K

更新日期：1999-08-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:Reaction center of chromatophores of Rhodospirillum rubrum consists of three kinds of protein, H-, M-, and L-subunit, and is bound with many other kinds of protein to form a larger protein complex (PRU; photoreaction unit), which contains all the bacteriochlorophyll. In the present study, purified PRU was dissociated ...

journal_title：Journal of biochemistry

authors： Tanaka K,Kakuno T,Yamashita J,Horio T

更新日期：1983-12-01 00:00:00

abstract：:The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extrac...

journal_title：Journal of biochemistry

authors： Igawa T,Higashi S,Abe Y,Ohkuri T,Tanaka H,Morimoto S,Yamashita T,Tsuda M,Inoue K,Ueda T

更新日期：2013-03-01 00:00:00

abstract：:Immunoblotting showed that a monoclonal antibody, 3A10, binds to a series of rat brain-specific antigens with molecular masses of 150-, 120-, 118-, 106-, 104-, 79-, and 77-kDa. The expression of 3A10 antigens is dependent on the developmental stage of the brain; only the 106-kDa antigen is detected during embryonic st...

journal_title：Journal of biochemistry

authors： Harada A,Takeuchi K,Dohmae N,Takio K,Uenaka T,Aoki J,Inoue K,Umeda M

更新日期：1999-03-01 00:00:00

abstract：:Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...

journal_title：Journal of biochemistry

authors： Fukuhara M,Nohmi T,Mizokami K,Sunouchi M,Ishidate M Jr,Takanaka A

更新日期：1989-08-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:A 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) binding site of mouse mastocytoma P815 cell membranes has been purified approximately 100-fold by affinity chromatography. This adenosine binding site, which has a similar specificity to that of the A2 adenosine receptor, was absorbed on NECA-linked Sepharose 6B and elut...

journal_title：Journal of biochemistry

authors： Nakata H

更新日期：1989-05-01 00:00:00

abstract：:Pig heart phosphoprotein phosphatase [phosphoprotein phosphophydrolase, EC 3.1.3.16] of Mr 224,000 was dissociated by gel-filtration on Sephacryl S-300, into an active subunit (alpha subunit) of Mr 31,000 and inactive subunits of higher molecular weight in the presence of 6 M urea. After the removal of urea, these sub...

journal_title：Journal of biochemistry

authors： Imazu M,Imaoka T,Usui H,Kinohara N,Takeda M

更新日期：1981-09-01 00:00:00

abstract：:Soybean Bowman-Birk inhibitor, a double-headed inhibitor of trypsin and alpha-chymotrypsin, was treated with cyanogen bromide and then pepsin to yield two inhibitory active fragments. Structural investigation showed that one of the fragments was derived from the trypsin inhibitory domain and the other from the chymotr...

journal_title：Journal of biochemistry

authors： Odani S,Ikenaka T

更新日期：1978-03-01 00:00:00

abstract：:1. The relation between intrinsic viscosity in 0.2 M NaCl and molecular weight for samples of chondroitin sulfate isolated from shark cartilage could be expressed by [eeta] = 6.5 X 10(-6) Mr114. 2. Their sedimentation coefficients were linear with respect to the square root of molecular weight, and the intercept was n...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-03-01 00:00:00

abstract：:Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, ...

journal_title：Journal of biochemistry

authors： Matsumura K,Hori S,Sugiura H,Shimizu T

更新日期：1990-10-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:Bacteriochlorophyll (Bchl) protein complexes containing light harvesting (LH) Bchls were isolated from Chromatium vinosum, and their CD spectra were measured in the near-infrared region. These isolated Bchl protein complexes retained the CD signals of LH Bchls that were observed in situ in chromatophores. The CD spect...

journal_title：Journal of biochemistry

authors： Hayashi H,Nozawa T,Hatano M,Morita S

更新日期：1981-06-01 00:00:00

abstract：:Sporulation of Bacillus subtilis, growing exponentially in the presence of rapidly metabolizable nutrients, was induced by addition of decoyinine (an antibiotic inhibitor of GMP synthesis), and intracellular amounts of ppGpp were determined after 2 M formic acid extraction by polyethyleneimine (PEI)-cellulose thin-lay...

journal_title：Journal of biochemistry

authors： Ikehara K,Okamoto M,Sugae K

更新日期：1982-03-01 00:00:00

abstract：:To reveal the molecular orientation of plastocyanin (PC) on spinach thylakoid membranes, the position of Lys residues modified by acetic anhydride was compared between thylakoid-bound PC and the isolated one. Digestion of the isolated PC by a trypsin yielded a peptide map with seven spots prior to the acetylation of t...

journal_title：Journal of biochemistry

authors： Takano M,Takahashi M,Oobatake M,Asada K

更新日期：1985-11-01 00:00:00

abstract：:Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

journal_title：Journal of biochemistry

authors： Asayama M,Kobayashi Y

更新日期：1993-09-01 00:00:00

abstract：:The terminal bases of the viral ribonucleic acid from the Caulobacter ribonucleic acid phage phiCp2 were examined. The viral ribonucleic acid contained guanosine triphosphate (pppG) at the 5'-terminus and adenosine (AOH) at the 3'-terminus. ...

journal_title：Journal of biochemistry

authors： Fujiki K,Fukuda A,Okada Y

更新日期：1978-07-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:In order to examine the possibility of the use of a surface plasmon resonance (SPR) sensor for real-time monitoring of the process of refolding of immobilized proteins, the refolding of firefly luciferase immobilized on a carboxymethyldextran matrix layer was analyzed. The SPR signal of the immobilized luciferase decr...

journal_title：Journal of biochemistry

authors： Zako T,Harada K,Mannen T,Yamaguchi S,Kitayama A,Ueda H,Nagamune T

更新日期：2001-01-01 00:00:00

abstract：:Agglutinin in the perivitelline fluid (PVF) of Tachypleus gigas, horseshoe crab, embryo was isolated and purified by a combination of affinity column chromatography on Sepharose 4B coupled with bovine submaxillary gland mucin and gel-filtration of Fractogel TSK (Toyopearl) HW-60 in Tris-NaCl-CaCl2 (0.05 M Tris-HCl, pH...

journal_title：Journal of biochemistry

authors： Shishikura F,Sekiguchi K

更新日期：1984-09-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:We studied the regulation of lipid body biogenesis in the oleaginous fungus Mortierella ramanniana var. angulispora by investigating culture conditions to modulate lipid body size, which we found was affected by the carbon-to-nitrogen ratio (C/N ratio) in the culture medium. Increasing the nitrogen source or decreasin...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Noda N,Yamaoka M

更新日期：2004-02-01 00:00:00