Dinuclear lanthanum(III) complex for efficient hydrolysis of RNA.

abstract：:Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...

journal_title：Journal of biochemistry

authors： Teshima K,Ikeda K,Hamaguchi K,Hayashi K

更新日期：1983-07-01 00:00:00

abstract：:Purothionin isolated from commercial wheat flour contained several components and two of them (A-I and A-II) were isolated in pure form by CM-52 column chromatography. Each component contained 45 amino acid residues with a 4 disulfide bonds. Purothionin A-II was digested with trypsin and thermolysin to isolate cystine...

journal_title：Journal of biochemistry

authors： Hase T,Matsubara H,Yoshizumi H

更新日期：1978-06-01 00:00:00

abstract：:Protein-tyrosine kinase p72syk exists abundantly in various hematopoietic cells and is activated by physiological or non-physiological agents. In this study we used vanadate, which is well known as a protein-tyrosine phosphatase inhibitor, to investigate the activity of p72syk and a downstream substrate for p72syk. Tr...

journal_title：Journal of biochemistry

authors： Nagai K,Inazu T,Yamamura H

更新日期：1994-11-01 00:00:00

abstract：:A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...

journal_title：Journal of biochemistry

authors： Kinoshita M,Okazaki M,Kato H,Teramoto T,Matsushima T,Naito C,Oka H,Hara I

更新日期：1983-08-01 00:00:00

abstract：:Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dic...

journal_title：Journal of biochemistry

authors： Moriyama Y,Takano T,Ohkuma S

更新日期：1982-10-01 00:00:00

abstract：:The cDNAs encoding the precursors of cystatin SN, cystatin S, and two mutants of cystatin S (-18R-->W; 117R-->W) were expressed in Escherichia coli JM109 with isopropyl-beta-D-thio-galactoside (IPTG) induction. Premature cystatin S with the original signal [-20MARPLCTLLLLMATLAGALA] was processed and a large amount of ...

journal_title：Journal of biochemistry

authors： Saitoh E,Isemura S

更新日期：1994-08-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:A proton nuclear magnetic resonance (NMR) study is reported of the molecular structural basis of antigen-antibody interactions. An immunologically reactive proteolytic fragment corresponding to one of the antigenic regions on hen egg-white lysozyme (HEL) was used in combination with a monoclonal antibody that recogniz...

journal_title：Journal of biochemistry

authors： Ito W,Nishimura M,Sakato N,Fujio H,Arata Y

更新日期：1987-09-01 00:00:00

abstract：:Glycerol-treated muscle fiber bundles were fixed at their rest length in 50 mM KC1, 2 mM MgC1(2), and 10 micron CaC1(2) at pH 7.8 and 0 degrees C in the presence of sufficient amounts of ATP, creatine kinase, and creatine phosphate. The fiber bundles were stretched linearly with time for 0.3 s at a constant amplitude,...

journal_title：Journal of biochemistry

authors： Arata T,Mukohata Y,Tonomura Y

更新日期：1978-10-01 00:00:00

abstract：:A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the att...

journal_title：Journal of biochemistry

authors： Miyagawa A,Okuyama T

更新日期：1977-06-01 00:00:00

abstract：:A gene for a methionine aminopeptidase (MAP; EC 3.4.11.18), which catalyzes the removal of amino-terminal methionine from the growing peptide chain on the ribosome, has been cloned from the hyperthermophilic Archaeon, Pyrococcus furiosus, by a novel method effectively using its cosmid protein library, sequenced and ex...

journal_title：Journal of biochemistry

authors： Tsunasawa S,Izu Y,Miyagi M,Kato I

更新日期：1997-10-01 00:00:00

abstract：:Activation of antiviral innate immunity is triggered by cellular pattern recognition receptors. Retinoic acid inducible gene-I (RIG-I)-like receptors (RLRs) detect viral non-self RNA in cytoplasm of virus-infected cells and play a critical role in the clearance of the invaded viruses through production of antiviral cy...

journal_title：Journal of biochemistry

authors： Yoneyama M,Jogi M,Onomoto K

更新日期：2016-03-01 00:00:00

abstract：:A method for preparation of pyridylamino (PA-) derivatives of O-linked sugar chains from glycoproteins was developed. A glycopeptide containing O-linked Gal beta 1-3GalNAc was prepared from fetuin and treated with anhydrous hydrazine followed by N-acetylation of free amino groups. Sugar chains released were pyridylami...

journal_title：Journal of biochemistry

authors： Kuraya N,Hase S

更新日期：1992-07-01 00:00:00

abstract：:After partial chromatographic purification of rat liver cell sap on DEAE-cellulose, including the removal of type M2 pyruvate kinase, different forms of type L pyruvate kinase were separated by chromatofocusing. Three fractions of pyruvate kinase activity were found, eluting at pH 5.0, 5.2, and 5.3, respectively. The ...

journal_title：Journal of biochemistry

authors： Ekdahl KN,Ekman P

更新日期：1984-04-01 00:00:00

abstract：:The molecular masses of two of the four DNA polymerase alpha-primase complex subunit peptides from various mammalian cells have been compared through the use of specific monoclonal antibodies. One monoclonal antibody (E4) binds to 77-kDa peptide from HeLa cells and cognate peptides from other mammalian cells (monkey, ...

journal_title：Journal of biochemistry

authors： Kozu T,Nikaido K,Yagura T

更新日期：1990-04-01 00:00:00

abstract：:The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 micrograms/mg DNA, which corresponds ...

journal_title：Journal of biochemistry

authors： Tamura H,Sekimizu K,Obinata M,Natori S

更新日期：1980-11-01 00:00:00

abstract：:Studies with crude preparations obtained from a cysteine auxotroph of Saccharomyces cerevisiae showed that O-acetylserine sulfhydrylase could be separated from O-acetylhomoserine sulfhydrylase by chromatography on a DEAE-cellulose column and centrifugation in a sucrose density gradient. On the basis of sedimentation d...

journal_title：Journal of biochemistry

authors： Yamagata S

更新日期：1980-11-01 00:00:00

abstract：:In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with...

journal_title：Journal of biochemistry

authors： Nakamura Y,Oda K,Nakada S

更新日期：1991-09-01 00:00:00

abstract：:Four flavonoids (i.e., baicalein, quercetin, quercetagetin, and myricetin), known to be inhibitors of HIV-reverse transcriptase, have been shown to be more or less inhibitory to the activities of various cellular DNA and RNA polymerases. The degree of the inhibition varied depending on the combination of the flavonoid...

journal_title：Journal of biochemistry

authors： Ono K,Nakane H

更新日期：1990-10-01 00:00:00

abstract：:Irradiation of isolated chloroplast thylakoids with TNP-ADP results in non-covalent binding and covalent incorporation of a reaction product of TNP-ADP formed by photosynthetic reduction into the so-called "tight" nucleotide binding site of CF1 [Ponse et al. (1992) Z. Naturforsch. 47c, 264-274]. CF1 extracted from thu...

journal_title：Journal of biochemistry

authors： Hisabori T,Kothen G,Strotmann H

更新日期：1993-09-01 00:00:00

abstract：:The promoter -10 binding region of the Bacillus subtilis sigma(A) factor forms an amphiphilic alpha-helix with three conserved isoleucines located at four-residue intervals. To investigate the structural and functional roles of the three isoleucine residues, we constructed a series of sigA mutants with single and doub...

journal_title：Journal of biochemistry

authors： Liao CT,Wang WH,Yang HS,Chen JP,Chang BY

更新日期：1999-09-01 00:00:00

abstract：:A hybridoma cell line producing mouse monoclonal antibody against pig kidney Na,K-ATPase was established. The antibody, named 38 (mAb38, IgG1), was purified from mouse ascites fluid by chromatography on a protein A-Sepharose column. Antigens immobilized on microplate wells with p-benzoquinone were used for titer assay...

journal_title：Journal of biochemistry

authors： Urayama O,Nagamune H,Nakao M,Hara Y,Sugiyama H,Sato K,Nakao T

更新日期：1985-07-01 00:00:00

abstract：:Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) mainly catalyzes oxygenation when L-phenylalanine is used as the substrate, but oxidation when L-methionine is used as the substrate. Using [C(alpha)-H]-DL-methionine and [C(alpha)-D]-DL-methionine as substrate, the reductive half reaction of FAD co...

journal_title：Journal of biochemistry

authors： Ohta Y,Mukouyama EB,Suzuki H

更新日期：2006-03-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:Histidine and its derivatives increased rabbit muscle fructose 1,6-bisphosphatase activity at neutral pH with positive cooperativity. In the presence of histidine and carnosine the optimum pH shifted from pH 8.0 to 7.4. The cooperative response of the enzyme to AMP and fructose 1,6-bisphosphate was observed in the pre...

journal_title：Journal of biochemistry

authors： Ikeda T,Kimura K,Hama T,Tamaki N

更新日期：1980-01-01 00:00:00

abstract：:Plasmapheresis with a dextran sulfate column is a treatment for patients with hypercholesteremia. When proteins bound to the column during the treatment were fractionated to prepare some known proteins, we found a 57 kDa glycoprotein designated GP57 which showed a new N-terminal amino acid sequence. Western-blot analy...

journal_title：Journal of biochemistry

authors： Choi-Miura NH,Sano Y,Oda E,Nakano Y,Tobe T,Yanagishita T,Taniyama M,Katagiri T,Tomita M

更新日期：1995-02-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...

journal_title：Journal of biochemistry

authors： Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IM

更新日期：1990-05-01 00:00:00

abstract：:Crystals as large as 0.5 X 0.3 X 0.2 mm of purified arginine-transfer RNA from Escherichia coli have been prepared by a vapour diffusion method. X-ray diffraction photographs showed that the crystals gave reflections up to 3.7 A spacing. They have a trigonal space group P31 2 1 (OR P32 2 1) and cell-dimensions a=97.2,...

journal_title：Journal of biochemistry

authors： Morikawa K,Sakamaki T,Nishimura Y,Mitsui Y,Aoki K,Iitaka Y,Tsuboi M,Nishimura S

更新日期：1978-08-01 00:00:00

abstract：:We isolated three genomic clones of rat P-450(11 beta) genes (CYP11B). Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively. The third one was a novel gene resembling both CYP11B1 and CYP11B2, and was named CYP11B3 gene (CYP11B3). CYP11B2 and CYP11B3 are ...

journal_title：Journal of biochemistry

authors： Nomura M,Morohashi K,Kirita S,Nonaka Y,Okamoto M,Nawata H,Omura T

更新日期：1993-02-01 00:00:00