Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test.

abstract：:The interactions of substoichiometric TNP-ATP and F1-ATPase from Escherichia coli (EF1) were examined and compared with those in the case of mitochondrial F1-ATPase (MF1) and F1-ATPase from thermophilic Bacillus PS3 (TF1). EF1 hydrolyzed substoichiometric TNP-ATP faster than TF1 or MF1, although some 20% of the TNP-AT...

journal_title：Journal of biochemistry

authors： Muneyuki E,Hisabori T,Sasayama T,Mochizuki K,Yoshida M

更新日期：1996-11-01 00:00:00

abstract：:The binding of SopA to the promoter region of its own gene, in which four copies of SopA's recognition sequence, 5'-CTTTGC-3', are arrayed asymmetrically, was examined in vitro. Titration using electrophoretic mobility shift assay showed that the stoichiometry of SopA protomers to the promoter-region DNA is 4 and that...

journal_title：Journal of biochemistry

authors： Komai M,Umino M,Hanai R

更新日期：2011-04-01 00:00:00

abstract：:Highly purified recombinant human tumor necrosis factor (TNF) (molecular mass determined as 17 kilodaltons (kDa) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and as 36 kDa by Sephadex G-100 gel chromatography) was labeled with 125I to a specific activity of 5 microCi/micrograms without appreciable loss...

journal_title：Journal of biochemistry

authors： Yoshie O,Tada K,Ishida N

更新日期：1986-09-01 00:00:00

abstract：:In the preceding papers, we showed that one of the two complementar factors of polypeptide chain elongation factor 1 (EF-1) from pig liver, EF-1alpha, functionally corresponds to bacterial EF-Tu (Nagata, S., Iwasaki, K., and Kaziro, Y. (1976) Arch. Biochem. Biophys. 172, 168), while the other, EF-1betagamma, as well a...

journal_title：Journal of biochemistry

authors： Nagata S,Motoyoshi K,Iwasaki K

更新日期：1978-02-01 00:00:00

abstract：:A post-proline cleaving enzyme [post-proline endopeptidase: EC 3.4.21.26] was purified from lamb brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and Sephadex G-150. The purified enzyme appeared homogeneous on disc gel and sodium dodecyl sulfate (SDS) gel electrophoreses. The enzyme was mo...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Simmons WH,Kita T,Tsuru D

更新日期：1981-08-01 00:00:00

abstract：:Loss of bone substance is a common manifestation of hyperparathyroidism. This suggests that parathyroid hormone (PTH) plays an important role as to bone mass. To investigate the mechanism underlying this change in bone mass, I studied the effects of PTH on collagen synthesis and mitogenesis of UMR-106 rat osteoblastic...

journal_title：Journal of biochemistry

authors： Pun KK

更新日期：1989-12-01 00:00:00

abstract：:We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

journal_title：Journal of biochemistry

authors： Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

更新日期：2008-12-01 00:00:00

abstract：:Upon rehydration of lyophilized Escherichia coli cells with phosphate buffer containing [14C]isopentenyl pyrophosphate (IPP), 14C was incorporated into the cells. Radioactivity was found in ubiquinone-8, an unidentified precursor of ubiquinone-8, demethylmenaquinone-8 and phosphate esters of all-trans-octaprenol and c...

journal_title：Journal of biochemistry

authors： Fujisaki S,Nishino T,Katsuki H

更新日期：1986-04-01 00:00:00

abstract：:Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

journal_title：Journal of biochemistry

authors： Takeda Y,Ohnishi T,Ogiso Y

更新日期：1976-09-01 00:00:00

abstract：:Ca(2+)/calmodulin-dependent protein kinases (CaM-kinases) I and IV are activated upon phosphorylation of their Thr(177) and Thr(196), respectively, by the upstream Ca(2+)/calmodulin-dependent protein kinases CaM-kinase kinase alpha and beta, and deactivated upon dephosphorylation by protein phosphatases such as CaM-ki...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：2001-10-01 00:00:00

abstract：:The nucleotide sequence of IStaqTZ2 are available in the DDBJ/EMBL/GenBank databases under the accession number AB063392. A novel insertion sequence (IStaqTZ2) was transposed from the genome of Thermus thermophilus TZ2 to that of the thermophilic bacteriophage IN93. The complete nucleotide sequence of IStaqTZ2 was det...

journal_title：Journal of biochemistry

authors： Matsushita I,Yanase H

更新日期：2009-12-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...

journal_title：Journal of biochemistry

authors： Abe T,Kawarai T,Takahashi Y,Konishi K

更新日期：2017-06-01 00:00:00

abstract：:The oxidation of equivalent concentrations of phospholipids in homogeneous solution, in multilamellar liposomal suspension, and in rat liver homogenate was carried out under aerobic conditions at 37 degrees C in order to examine the biochemical fate of oxidized phospholipids. Rat liver phospholipids were extracted wit...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Yamashita S,Niki E,Yamamoto Y

更新日期：1997-03-01 00:00:00

abstract：:Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology a...

journal_title：Journal of biochemistry

authors： Lu JF,Pokharel D,Padula MP,Bebawy M

更新日期：2016-11-01 00:00:00

abstract：:We have studied the protein composition of the pectoralis superficialis muscle of genetically dystrophic (New Hampshire line 413) and normal control (line 412) chickens by one- and two-dimensional gel electrophoresis. A protein, referred to hereafter as the 30 kDa abnormal protein, was specifically detected in the aff...

journal_title：Journal of biochemistry

authors： Kobayashi R,Toyoshima I,Masamune O,Tashima Y

更新日期：1990-01-01 00:00:00

abstract：:Interactions between phospholipid membranes and the acyl chain and specific amino acid residues of myristoylated proteins are necessary for membrane association. In the present study we tested the effects of mutations of calcineurin B subunit amino acid residues K(20)K(21), K(24)R(25), K(27)K(28) to Glu on the interac...

journal_title：Journal of biochemistry

authors： Martin BA,Oxhorn BC,Rossow CR,Perrino BA

更新日期：2001-05-01 00:00:00

abstract：:We purified an antimicrobial protein of 76 residues, denoted bovine antimicrobial protein-1 (BAMP-1), from fetal calf serum using hydrophobic chromatography, gel filtration, and reverse-phase high-performance liquid chromatography. The amino acid sequence of BAMP-1 was similar to that of human apolipoprotein A-II (apo...

journal_title：Journal of biochemistry

authors： Motizuki M,Itoh T,Yamada M,Shimamura S,Tsurugi K

更新日期：1998-04-01 00:00:00

abstract：:Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. Carbohydrate analyses showed that the catalase was a glycoprotein containing a...

journal_title：Journal of biochemistry

authors： Kikuchi-Torii K,Hayashi S,Nakamoto H,Nakamura S

更新日期：1982-11-01 00:00:00

abstract：:We studied enzymatic activities in sea urchin egg extracts that phosphorylate myosin regulatory light chain (MRLC) from chicken gizzard smooth muscle. The activity in the presence of EGTA showed cell cycle-dependent changes similar to that of histone H1 kinase, namely, it peaked shortly before cleavage, while that in ...

journal_title：Journal of biochemistry

authors： Mishima M,Mabuchi I

更新日期：1996-05-01 00:00:00

abstract：:The conformation of the chondroitin-4-sulfate calcium salt was investigated by X-ray analysis. The following results were obtained. 1, The repeat length per disaccharide was 0.913 nm: 2, The molecular chain had three-fold screw symmetry: 3, The shape of the unit cell was a trigonal prism with dimensions a=b=1.28 nm, c...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-01-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:Vasoactive intestinal peptide (VIP), a neuropeptide coupled with adenylate cyclase, was found to induce neurite extension of PC12h cells. Neurites appeared within 1 h after addition of VIP and extended for at least 24 h. The half-maximal concentration for the effect of VIP was 50 nM. In addition to the morphological c...

journal_title：Journal of biochemistry

authors： Okumura N,Miyatake Y,Takao T,Tamaru T,Nagai K,Okada M,Nakagawa H

更新日期：1994-02-01 00:00:00

abstract：:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. Th...

journal_title：Journal of biochemistry

authors： Uchikoba T,Yonezawa H,Kaneda M

更新日期：1995-05-01 00:00:00

abstract：:To identify functionally essential sequences and residues of CTLA-2alpha, in vitro mutagenesis was carried out. The coefficient of inhibition (K(i)) was determined towards rabbit cathepsin L using Z-Phe-Arg-MCA as the substrate. Recombinant CTLA-2alpha inhibited the enzyme potently (K(i) = 15 nM). A truncated mutant, ...

journal_title：Journal of biochemistry

authors： Deshapriya RM,Yuhashi S,Usui M,Kageyama T,Yamamoto Y

更新日期：2010-03-01 00:00:00

abstract：:The kinetics of the hydrogen-deuterium exchange reaction in sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle was followed by infrared absorption measurements. The exchange rate in SR was much lower than that in the soluble proteins reported so far. When adenylyl-imidophosphate (AMPPNP, an TP ...

journal_title：Journal of biochemistry

authors： Anzai K,Kirino Y,Shimizu H

更新日期：1981-08-01 00:00:00

abstract：:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamo-Yamada F,Cho S,Sugano M

更新日期：1980-05-01 00:00:00

abstract：:A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

journal_title：Journal of biochemistry

authors： Sone N,Kutoh E,Sato K

更新日期：1990-04-01 00:00:00

abstract：:Two kinds of monoclonal antibodies (3B9 and SM1) against connectin, muscle elastic protein, reacted with both alpha- and beta-connectins. Immunofluorescence studies revealed that 3B9 stained both edges of the A band of chicken breast muscle myofibrils and remained as such upon stretching to a sarcomere length of 3.5 m...

journal_title：Journal of biochemistry

authors： Itoh Y,Suzuki T,Kimura S,Ohashi K,Higuchi H,Sawada H,Shimizu T,Shibata M,Maruyama K

更新日期：1988-10-01 00:00:00

abstract：:1. Porcine cardiac native tropomyosin was phosphorylated by bovine cardiac 3':5'-cyclic AMP-dependent protein kinase. Most of the phosphate incorporation was observed in troponin I, the maximum of which was 0.7 mol of Pi per mol of troponin I. 2. In the presence of phosphorylated native tropomyosin, actomyosin ATPase ...

journal_title：Journal of biochemistry

authors： Yamamoto K,Ohtsuki I

更新日期：1982-05-01 00:00:00

abstract：:The effects of sphingoid bases, sphingosine and dihydrosphingosine, which are protein kinase C (PKC) inhibitors, on NADPH oxidase were examined in a cell-free system. The bases inhibited cell-free activation of NADPH oxidase by arachidonic acid at lower concentration than N-acetylsphingosine. Thus, positive charge in ...

journal_title：Journal of biochemistry

authors： Sasaki JI,Yamaguchi M,Saeki S,Yamane H,Okamura N,Ishibashi S

更新日期：1996-10-01 00:00:00