The importance of parathyroid hormone in inhibition of collagen synthesis and mitogenesis of osteoblastic cell.

abstract：:Fibronectin was isolated from human placenta tissues and compared with human plasma fibronectin. Placenta and plasma fibronectins had similar amino acid compositions, immunological properties, and cell attachment-promoting activities, but differed in apparent molecular weight on sodium dodecyl sulfate-polyacrylamide g...

journal_title：Journal of biochemistry

authors： Isemura M,Yamaguchi Y,Munakata H,Aikawa J,Kan M,Yamane I,Yosizawa Z

更新日期：1984-07-01 00:00:00

abstract：:Fluorescence resonance energy transfer spectroscopy has been used to study the spatial relationships between probes attached to actin and troponin in the reconstituted skeletal muscle thin filament in the presence and absence of Ca2+ ions. Gln-41 and the nucleotide-binding site of actin were selectively labeled with t...

journal_title：Journal of biochemistry

authors： Miki M,Kobayashi T,Kimura H,Hagiwara A,Hai H,Maéda Y

更新日期：1998-02-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...

journal_title：Journal of biochemistry

authors： Miyanishi T,Toyoshima C,Wakabayashi T,Matsuda G

更新日期：1988-03-01 00:00:00

abstract：:Identification of single-stranded regions in Torulopsis utilis 5S RNA was attempted by the use of Nuclease S1, a single-strand specific endonuclease. When T. utilis 5S RNA was subjected to prolonged incubation with Nuclease S1, about 50% of the substrate 5S RNA remained as large oligonucleotide "cores." Such Nuclease...

journal_title：Journal of biochemistry

authors： Nishikawa K,Takemura S

更新日期：1977-04-01 00:00:00

abstract：:We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

journal_title：Journal of biochemistry

authors： Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

更新日期：2011-09-01 00:00:00

abstract：:Three kinds of phospholipase C [EC 3.1.4.3] were used to selectively hydrolyze phospholipids in rat liver microsomes, and their effects on the acyl-CoA: glycerophosphate and acyl-CoA: lysophospholipids acyltransferase systems were examined. The glycerophosphate acyltransferase [EC 2.3.1.15] system was inactivated rapi...

journal_title：Journal of biochemistry

authors： Sonoki S,Ikezawa H

更新日期：1976-12-01 00:00:00

abstract：:In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

journal_title：Journal of biochemistry

authors： Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

更新日期：1987-08-01 00:00:00

abstract：:Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

journal_title：Journal of biochemistry

authors： Shishido H,Yamada MD,Kondo K,Maruta S

更新日期：2009-10-01 00:00:00

abstract：:A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono Q. The enzyme had a...

journal_title：Journal of biochemistry

authors： Ohmoto T,Kinoshita T,Moriyoshi K,Sakai K,Hamada N,Ohe T

更新日期：1998-09-01 00:00:00

abstract：:A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activi...

journal_title：Journal of biochemistry

authors： Maeshima M,Asahi T

更新日期：1981-08-01 00:00:00

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:Bdellin-KL is a trypsin-plasmin inhibitor from Hirudo nipponia, whose N-terminal sequence was identified as a non-classical Kazal-type. A cDNA clone encoding the inhibitor was isolated by reverse transcription-PCR and 5' rapid amplification of cDNA ends. The cDNA showed an open reading frame of 155 amino acids compris...

journal_title：Journal of biochemistry

authors： Kim YH,Choi JG,Lee GM,Kang KW

更新日期：2001-09-01 00:00:00

abstract：:Several synthetic peptides derived from the C-terminal domain sequence of a hemolytic lectin, CEL-III, were examined as to their action on bacteria and artificial lipid membranes. Peptide P332 (KGVIFAKASVSVKVTASLSK-NH(2)), corresponding to the sequence from residue 332, exhibited strong antibacterial activity toward G...

journal_title：Journal of biochemistry

authors： Hatakeyama T,Suenaga T,Eto S,Niidome T,Aoyagi H

更新日期：2004-01-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an inte...

journal_title：Journal of biochemistry

authors： Nagai A,Ohta D

更新日期：1994-01-01 00:00:00

abstract：:LukF and Hlg2 of staphylococcal gamma-hemolysin assemble into hetero-oligomeric pores on human red blood cells (HRBC). Here, we demonstrate, using a single-molecule imaging technique, that a W177T/R198T mutant of LukF, which exhibits no binding activity toward phosphatidylcholine, could form intermediate oligomers wit...

journal_title：Journal of biochemistry

authors： Monma N,Nguyen VT,Kaneko J,Higuchi H,Kamio Y

更新日期：2004-10-01 00:00:00

abstract：:We have previously purified two D-3-hydroxyacyl-CoA dehydratase preparations from human liver. One preparation contained a 77-kDa polypeptide and smaller polypeptides, and the other was a homodimer of a 46-kDa polypeptide. Three different purified rat peroxisomal D-3-hydroxyacyl-CoA dehydratase preparations have been ...

journal_title：Journal of biochemistry

authors： Jiang LL,Miyazawa S,Hashimoto T

更新日期：1996-09-01 00:00:00

abstract：:Alanine-glyoxylate aminotransferase (AGT) 2 is a pyridoxal 5'-phosphate dependent, mitochondrial enzyme which, in the rat, is expressed at a high level in the kidney. The amino acid sequences of nine tryptic and seven CNBr peptides of the rat kidney AGT2 were determined. Three overlapping cDNAs encoding the AGT2 were ...

journal_title：Journal of biochemistry

authors： Lee IS,Muragaki Y,Ideguchi T,Hase T,Tsuji M,Ooshima A,Okuno E,Kido R

更新日期：1995-04-01 00:00:00

abstract：:The essential gene product Prp45 (379 aa) of Saccharomyces cerevisiae is a highly conserved, but N-terminally abridged, ortholog of the human transcriptional coactivator SKIP, which is involved in TGFbeta, Notch, and steroid hormone signaling. We used a diploid strain harboring PRP45 deletion, which is inviable in the...

journal_title：Journal of biochemistry

authors： Martinkova K,Lebduska P,Skruzny M,Folk P,Puta F

更新日期：2002-10-01 00:00:00

abstract：:Isolation and characterization of a fibrous protein component which might be associated with contractile ring of a dividing Tetrahymena cell were attempted by making use of coprecipitation of the protein with rabbit skeletal muscle myosin. The protein was purified to homogeneity by ammonium sulfate fractionation betwe...

journal_title：Journal of biochemistry

authors： Numata O,Yasuda T,Hirabayashi T,Watanabe Y

更新日期：1980-11-01 00:00:00

abstract：:The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed step in the biosynthesis of aromatic amino acids and vitamins. Through target-directed mutagenesis of the cloned aroG on a p...

journal_title：Journal of biochemistry

authors： Ger YM,Chen SL,Chiang HJ,Shiuan D

更新日期：1994-11-01 00:00:00

abstract：:The effects of various divalent cations on the Ca2+ uptake by microsomes from bovine aortic smooth muscle were studied. High concentrations (1 mM) of Co2+, Zn2+, Mn2+, Fe2+, and Ni2+ inhibited neither the Ca2+ uptake by the microsomes nor the formation of the phosphorylated intermediate (E approximately P) of the Ca2+...

journal_title：Journal of biochemistry

authors： Sumida M,Hamada M,Takenaka H,Hirata Y,Nishigauchi K,Okuda H

更新日期：1986-09-01 00:00:00

abstract：:Mouse epidermal growth factor (EGF) was covalently conjugated with the water-soluble polymer, poly(acrylic acid) (EGF-PAA), or with the water-insoluble polymer, surface-hydrolyzed poly(methyl methacrylate) (EGF-PMMA). Immobilized EGF (EGF-PMMA) stimulated DNA synthesis in Chinese hamster ovary cells overexpressing EGF...

journal_title：Journal of biochemistry

authors： Ito Y,Li JS,Takahashi T,Imanishi Y,Okabayashi Y,Kido Y,Kasuga M

更新日期：1997-03-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00

abstract：:Caenorhabditis elegans HMG-5, which is encoded by F45E4.9, contains two high mobility group (HMG) box domains and shows sequence similarity with mammalian mitochondrial transcription factor A (TFAM). In this study, using soaking RNA interference, we found that knockdown of HMG-5 reduced the amount of mtDNA in P0 herma...

journal_title：Journal of biochemistry

authors： Sumitani M,Kasashima K,Matsugi J,Endo H

更新日期：2011-05-01 00:00:00

abstract：:Previously, we purified the cathepsin B mRNA 3'-untranslated-region-binding protein (CBBP) from Sarcophaga and suggested its participation in the translational regulation of cathepsin B mRNA in this insect. In this study, we isolated a full length cDNA for CBBP. CBBP was an RNA-binding protein that contained four RGG ...

journal_title：Journal of biochemistry

authors： Nishikawa T,Kobayashi A,Natori S

更新日期：2001-03-01 00:00:00

abstract：:The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...

journal_title：Journal of biochemistry

authors： Okazaki N,Hazeki K,Izumi T,Nigorikawa K,Hazeki O

更新日期：2011-03-01 00:00:00

abstract：:A simple and rapid preparation method for apoaspartate aminotransferase from Escherichia coli B was developed. A crude extract of the bacterial cells was treated batchwise with DEAE-cellulose. The enzyme fraction obtained was then applied to a pyridoxamine-Sepharose column. Apoaspartate aminotransferase was eluted wit...

journal_title：Journal of biochemistry

authors： Yagi T,Kirino J,Yamamoto S,Nozaki M

更新日期：1985-10-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00