当前位置： SCI文献检索 > JOURNAL OF BIOCHEMISTRY期刊下所有文献 > Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability.

Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability.

Abstract：

:The essential gene product Prp45 (379 aa) of Saccharomyces cerevisiae is a highly conserved, but N-terminally abridged, ortholog of the human transcriptional coactivator SKIP, which is involved in TGFbeta, Notch, and steroid hormone signaling. We used a diploid strain harboring PRP45 deletion, which is inviable in the haploid, to test for complementation with the truncated versions of Prp45. The N-terminal half of the protein (aa 1 to 190), denoted as the SNW domain, was found sufficient to support the essential function. Interestingly, substituting the SNW motif itself with AAA was compatible with viability. GFP-tagged Prp45 was localized in nuclear "speckles" over a diffuse nuclear background. We further found that Prp45 activated the transcription of a reporter gene in S. cerevisiae when targeted to DNA. The observed effect relied in part upon the presence of conserved helical repeats and upon the highly charged C-terminal domain (pI = 11.3). Prp45, which lacks most of the binding motifs of the human ortholog, and whose N-terminal half is sufficient for supporting the growth of prp45 cells, might be helpful in elucidating the essential function of SNW/SKIP proteins.

journal_name

J Biochem

journal_title

Journal of biochemistry

authors

Martinkova K,Lebduska P,Skruzny M,Folk P,Puta F

doi

10.1093/oxfordjournals.jbchem.a003257

keywords:

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

557-63

issue

4

eissn

0021-924X

issn

1756-2651

journal_volume

132

pub_type

杂志文章

相关文献

JOURNAL OF BIOCHEMISTRY文献大全
  • The human PTFgamma/SNAP43 gene: structure, chromosomal location, and identification of a VNTR in 5'-UTR.

    abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a022085

    authors： Maeng JH,Yoon JB

    更新日期：1998-07-01 00:00:00

  • Monoclonal antibodies for human thrombomodulin which recognize binding sites for thrombin and protein C.

    abstract：:Monoclonal antibodies for human thrombomodulin, a cofactor for thrombin-catalyzed activation of protein C, were prepared and their epitopes characterized. All six antibodies (MFTM-1-MFTM-6) bound to an elastase-digested active fragment of thrombomodulin, which contains six consecutive EGF domains. Binding of thrombomo...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a123295

    authors： Hayashi T,Suzuki K

    更新日期：1990-11-01 00:00:00

  • Characterization of hyaluronan-binding proteins on guinea pig polymorphonuclear leukocytes: possible involvement of complement receptor type 3 (CR3, CD11b/CD18) in the hyaluronan-leukocyte interaction.

    abstract：:Hyaluronan (HA), a high-molecular-weight glycosaminoglycan ubiquitously present in the extracellular matrices (ECMs) of animals, plays important roles in ECM organization and cell behavior through binding to hyaluronan-binding proteins (HABPs). We previously reported that HA has anti-inflammatory effects on guinea pig...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/mvj002

    authors： Nochi H,Shinomiya T,Tamoto K

    更新日期：2006-01-01 00:00:00

  • Fatty acids bound to recombinant tear lipocalin and their role in structural stabilization.

    abstract：:A variant of human tear lipocalin was expressed in Escherichia coli, and the bound fatty acids were analysed by gas chromatography, mass spectroscopy and nuclear magnetic resonance spectroscopy. Five major fatty acids were identified as hexadecanoic acid (palmitic acid, PA), cis-9-hexadecenoic acid (palmitoleic acid),...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/mvp076

    authors： Tsukamoto S,Fujiwara K,Ikeguchi M

    更新日期：2009-09-01 00:00:00

  • Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.

    abstract：:In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a123592

    authors： Nakamura Y,Oda K,Nakada S

    更新日期：1991-09-01 00:00:00

  • Affinity purification and characterization of serine hydroxymethyltransferases from rat liver.

    abstract：:A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC 2.1.2.1]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,6...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/101.3.643

    authors： Masuda T,Sakamoto M,Nishizaki I,Hayashi H,Yamamoto M,Wada H

    更新日期：1987-03-01 00:00:00

  • ATPase activity and filament formation of partially purified myosin from leucocytes.

    abstract：:Myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. The Ca2+-, EDTA-, and Mg2+-ATPase [EC 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 M KCl at pH 7.0 and 25 degrees. The Ca2+-AT...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：

    authors： Takeuchi K,Shibata N,Senda N

    更新日期：1975-07-01 00:00:00

  • Prospect of divergent roles for the CUL3 system in vascular endothelial cell function and angiogenesis.

    abstract：:Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogeni...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章,评审

    doi：10.1093/jb/mvx051

    authors： Sakaue T,Maekawa M,Nakayama H,Higashiyama S

    更新日期：2017-10-01 00:00:00

  • Interaction of sodium and potassium ions with Na+, K+-ATPase. III. Cooperative effect of ATP and Na+ on complete release of K+ from E2K.

    abstract：:The effects of Na+ and ATP on the K+ binding to Na+, K+-ATPase were investigated by the centrifugation method with radioactive K+ in the absence of Mg2+. In the presence of 10 microM 43KCl, 0.6 and 10 mM Na+ decreased the amount of bound K+ to one-half and zero, respectively. On the other hand, 10 microM and 10 mM ATP...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/101.3.789

    authors： Homareda H,Nozaki T,Matsui H

    更新日期：1987-03-01 00:00:00

  • Isolation of a stable tetramer of the low molecular weight component of neurofilaments (NF-L).

    abstract：:When crude neurofilaments were dissolved in a solution containing 8 M urea and 1% beta-mercaptoethanol (beta-ME), the component proteins of the neurofilaments and other contaminating filaments were solubilized into monomeric forms. However, when reassembled filaments were solubilized again by the addition of urea to 8...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a122614

    authors： Tokutake S,Tanaka K

    更新日期：1989-01-01 00:00:00

  • Proton nuclear magnetic resonance study of a selectively deuterated mouse monoclonal antibody: use of two-dimensional homonuclear Hartmann-Hahn spectroscopy.

    abstract：:A 1H NMR study of a selectively deuterated mouse anti-dansyl monoclonal antibody is reported. Two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy was found to be effective for establishing the connectivity between the C2-H and C4-H protons of His residues in the antibody molecule. It has been concluded ...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a122858

    authors： Kato K,Nishimura Y,Waelchli M,Arata Y

    更新日期：1989-09-01 00:00:00

  • DNA synthesis by fragment assembly using extra-cellular DNA delivered by artificial controlled horizontal transfer.

    abstract：:DNA synthesis in the Bacillus subtilis cells has become possible using extra-cellular DNA. Generally, purified DNAs in a test tube have been required to introduce into the host cells for molecular cloning technology in the laboratory. We have developed a cell lysis technique for natural transformation using stable ext...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/mvx085

    authors： Kaneko S,Fukushima H,Nakahama M,Asano S,Miyazaki Y,Aizawa Y,Itaya M

    更新日期：2018-04-01 00:00:00

  • The structure and function of acid proteases. V. Comparative studies on the specific inhibition of acid proteases by diazoacetyl-DL-norleucine methyl ester, 1,2-epoxy-3-(p-nitrophenoxy) propane and pepstatin.

    abstract：:Comparative studies have been made on the effects of diazoacetyl-DL-norleucine methyl ester (DAN), 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and pepstatin on acid proteases, including those from Acrocylindrium sp., Aspergillus niger, Aspergillus saitoi, Mucor pusillus, Paecilomyces varioti, Rhizopus chinensis, and Tr...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a131304

    authors： Takahashi K,Chang WJ

    更新日期：1976-09-01 00:00:00

  • Human spleen histone H2B. Isolation and amino acid sequence.

    abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a132371

    authors： Ohe Y,Hayashi H,Iwai K

    更新日期：1979-02-01 00:00:00

  • Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus-31 nuclear magnetic resonance.

    abstract：:Myosin subfragment-1.pyrophosphate (S1.PPi) complex and S1.ADP complex were observed with 31P NMR at various temperatures between 0 and 25 degrees C. The signal of S1.PPi complex showed a small temperature dependence, indicating that a single conformation exists for the complex. It also showed that the electron densit...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a123996

    authors： Tanokura M,Ebashi S

    更新日期：1993-01-01 00:00:00

  • Drosophila melanogaster aldolase: characterization of the isozymes alpha, beta, and gamma generated from a single gene.

    abstract：:Three isozymic forms, alpha, beta, and gamma, of Drosophila melanogaster aldolase are produced from a single gene by alternative usage of the triple exons 4 (4 alpha, 4 beta, and 4 gamma) [Shaw-Lee et al. (1992) J. Biol. Chem. 267, 3959-3967; Kim et al. (1992) Mol. Cell. Biol. 12, 773-783; Kai et al. (1992) J. Biochem...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a124876

    authors： Zhang R,Kai T,Sugimoto Y,Kusakabe T,Takasaki Y,Koga K,Hori K

    更新日期：1995-07-01 00:00:00

  • Amino acid sequence and attachment sites of oligosaccharide units of porcine erythrocyte glycophorin.

    abstract：:The amino acid sequence of the glycophorin from porcine erythrocyte membrane has been determined by Edman degradation. Porcine glycophorin is a polypeptide chain of 133 amino acid residues and contains 12 oligosaccharide units attached to the amino-terminal side of the molecule. Ten oligosaccharides are linked O-glyco...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a133143

    authors： Honma K,Tomita M,Hamada A

    更新日期：1980-12-01 00:00:00

  • Primary structure of hemorrhagic protein, HR2a, isolated from the venom of Trimeresurus flavoviridis.

    abstract：:The complete amino acid sequence and disulfide bridge location of HR2a, one of the hemorrhagic proteins isolated from the snake venom of Trimeresurus flavoviridis, have been determined by analysis of peptides derived from digests with cyanogen bromide, lysyl endopeptidase, trypsin, and Staphylococcus aureus V8 proteas...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a122756

    authors： Miyata T,Takeya H,Ozeki Y,Arakawa M,Tokunaga F,Iwanaga S,Omori-Satoh T

    更新日期：1989-05-01 00:00:00

  • Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus).

    abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a124099

    authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

    更新日期：1993-06-01 00:00:00

  • Cloning and expression of the MutM gene from obligate anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F).

    abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/jb/mvp005

    authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

    更新日期：2009-04-01 00:00:00

  • Fluorometric investigation of the local conformation of 16S rRNA in the regions of the 3'- and 5'-ends.

    abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a131094

    authors： Kumagai JI,Ohta T,Imahori K

    更新日期：1976-03-01 00:00:00

  • ATP-dependent uptake of anti-neoplastic agents by acidic organelles.

    abstract：:Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organell...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a124320

    authors： Moriyama Y,Manabe T,Yoshimori T,Tashiro Y,Futai M

    更新日期：1994-02-01 00:00:00

  • Instability of bacteriophage lambda initiator O and P proteins in DNA replication.

    abstract：:Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a134360

    authors： Miwa T,Akaboshi E,Matsubara K

    更新日期：1983-08-01 00:00:00

  • Isolation and characterization of a lectin from edible mushroom, Volvariella volvacea.

    abstract：:A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1% 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of t...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a134826

    authors： Lin JY,Chou TB

    更新日期：1984-07-01 00:00:00

  • Oxidation and oxygenation of L-amino acids catalyzed by a L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501.

    abstract：:A number of L-amino acids and derivatives were tested as substrates for the purified Pseudomonas L-phenylalanine oxidase. The reaction products of these amino acids were analyzed by high performance liquid chromatography and the kinetic properties of the reactions were partially characterized. In addition to L-phenyla...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a134853

    authors： Koyama H

    更新日期：1984-08-01 00:00:00

  • Involvement of free ribosomes in the early stage of secretory protein biosynthesis in rat liver.

    abstract：:Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin na...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a133348

    authors： Oda T,Ito A

    更新日期：1981-05-01 00:00:00

  • Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida.

    abstract：:Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a122939

    authors： Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

    更新日期：1989-11-01 00:00:00

  • Synthesis of protein C in human umbilical vein endothelial cells.

    abstract：:By monitoring the activation of protein C and the regulation of factor Xa-catalyzed thrombin formation by the activated protein C (APC) on the surface of human umbilical vein endothelial cells (HUVEC), we found that functional protein C was synthesized in cultured HUVEC and expressed thereon in the presence of vitamin...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a123481

    authors： Tanabe S,Sugo T,Matsuda M

    更新日期：1991-06-01 00:00:00

  • Differential rate assay of human pancreatic and salivary alpha-amylases in serum using two coupled enzymes.

    abstract：:p-Nitrophenyl O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha- D-glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-alpha-D-glucopyranoside (FG5P) is hydrolyzed by human pancreatic a-amylase (HPA) or salivary alpha-amylase (HSA) to O-6-deoxy-6-[(2-pyridy...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：10.1093/oxfordjournals.jbchem.a121841

    authors： Omichi K,Ikenaka T

    更新日期：1986-11-01 00:00:00

  • Studies on trypsin inhibitor in barley. I. Purification and some properties.

    abstract：:To clarify the properties and functions of a trypsin inhibitor from Japanese barley in comparison with the inhibitor from Pirkka barley, an inhibitor was isolated from the barley Hordeum distichum L var. emend Lamark by extraction with 1% NaCl, ammonium sulfate fractionation and repeated chromatography on DEAE-cellul...

    journal_title：Journal of biochemistry

    pub_type： 杂志文章

    doi：

    authors： Ogiso T,Noda T,Sako Y,Kato Y,Aoyama M

    更新日期：1975-07-01 00:00:00