Cleavage specificity of cucumisin, a plant serine protease.


:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. The favorable synthetic substrates for cucumisin were Glt-Ala-Ala-Pro-Leu-pNA and Suc-Ala-Ala-Pro-Phe-pNA. The constant (kcat/Km) for Suc-Ala-Pro-Ala-pNA was found to be 30 times greater than that for Suc-Ala-Ala-Ala-pNA. The substrate specificity of cucumisin for oligopeptides and proteins was shown to be broad.


J Biochem


Journal of biochemistry


Uchikoba T,Yonezawa H,Kaneda M




Has Abstract


1995-05-01 00:00:00












  • Transformation of leukotriene D4 catalyzed by lysosomal cathepsin H of rat liver.

    abstract::Among several intracellular protease tested, cathepsin H transformed leukotriene D4 to E4 with a release of glycine in a stoichiometric quantity. Under the optimal conditions the rate of leukotriene D4 transformation by cathepsin H was about 3% of the hydrolysis rate of alpha-N-benzoyl-DL-arginine-2-naphthylamide whic...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yokota K,Shono F,Yamamoto S,Kominami E,Katunuma N

    更新日期:1983-10-01 00:00:00

  • Nucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver.

    abstract::Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Komiya H,Takemura S

    更新日期:1979-10-01 00:00:00

  • Subcellular distribution of GTP-binding proteins, Go and Gi2, in rat cerebral cortex.

    abstract::The localization of GTP-binding protein (G-protein) subunits, Go alpha, Gi2 alpha and beta, in subcellular fractions of rat cerebral cortex was determined by means of immunoassays specific for the respective subunits. High concentrations of all three subunits were observed in both crude mitochondrial and microsomal fr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Asano T,Nagahama M,Kato K

    更新日期:1990-05-01 00:00:00

  • Transmethylation reaction is essential for Sarcophaga lectin gene activation.

    abstract::Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugiyama H,Natori S

    更新日期:1996-02-01 00:00:00

  • RNF8 induces autophagy and reduces inflammation by promoting AKT degradation via ubiquitination in ulcerative colitis mice.

    abstract::RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

    更新日期:2020-11-01 00:00:00

  • Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus-31 nuclear magnetic resonance.

    abstract::Myosin subfragment-1.pyrophosphate (S1.PPi) complex and S1.ADP complex were observed with 31P NMR at various temperatures between 0 and 25 degrees C. The signal of S1.PPi complex showed a small temperature dependence, indicating that a single conformation exists for the complex. It also showed that the electron densit...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tanokura M,Ebashi S

    更新日期:1993-01-01 00:00:00

  • Effect of non-native helix destabilization on the folding of equine β-lactoglobulin.

    abstract::β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Okabe T,Miyajima T,Nakagawa K,Tsukamoto S,Fujiwara K,Ikeguchi M

    更新日期:2014-11-01 00:00:00

  • gamma-Carboxyglutamic acid (Gla)-domainless blood coagulation factor IXa species: preparation and properties.

    abstract::To investigate the function of the gamma-carboxyglutamic acid (Gla) residues of factor IXa in the activation of factor X, a new species of bovine factor IXa, designated "factor IXa beta'," and its corresponding Gla-domainless form, designated "Gla-domainless factor IXa beta'," were prepared under controlled conditions...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morita T,Fukudome K,Miyata T,Iwanaga S

    更新日期:1991-12-01 00:00:00

  • Site-directed mutagenesis of a thermostable alpha-amylase from Bacillus stearothermophilus: putative role of three conserved residues.

    abstract::The relationship between structure, activity, and stability of the thermostable Bacillus stearothermophilus alpha-amylase was studied by site-directed mutagenesis of the three most conserved residues. Mutation of His-238 to Asp involved in Ca2+ and substrate binding reduced the specific activity and thermal stability,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Vihinen M,Ollikka P,Niskanen J,Meyer P,Suominen I,Karp M,Holm L,Knowles J,Mäntsälä P

    更新日期:1990-02-01 00:00:00

  • Essential residues, W177 and R198, of LukF for phosphatidylcholine-binding and pore-formation by staphylococcal gamma-hemolysin on human erythrocyte membranes.

    abstract::LukF and Hlg2 of staphylococcal gamma-hemolysin assemble into hetero-oligomeric pores on human red blood cells (HRBC). Here, we demonstrate, using a single-molecule imaging technique, that a W177T/R198T mutant of LukF, which exhibits no binding activity toward phosphatidylcholine, could form intermediate oligomers wit...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Monma N,Nguyen VT,Kaneko J,Higuchi H,Kamio Y

    更新日期:2004-10-01 00:00:00

  • Unwinding of DNA by nonhistone chromosomal protein HMG(1 + 2) from pig thymus as determined with endonuclease.

    abstract::A high mobility group (HMG) nonhistone protein fraction HMG(1 + 2), composed of HMG1 and HMG2, was prepared from pig thymus chromatin. In order to examine a possibility that the HMG(1 + 2) participates in the unwinding of the DNA double-helix, DNA hydrolysis assay systems with the endonucleases specific for single-str...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yoshida M,Shimura K

    更新日期:1984-01-01 00:00:00

  • Restoration of Ca2+-transport in sarcoplasmic reticulum ATPase solubilized with octaethyleneglycol mono n-dodecyl ether.

    abstract::One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Andoh R,Yamamoto T

    更新日期:1985-03-01 00:00:00

  • Two distinct leukotriene B4 receptors, BLT1 and BLT2.

    abstract::Leukotriene B4 (LTB4) is a potent inflammatory mediator derived from arachidonic acid. Two G protein-coupled receptors for LTB4 have been identified: a high-affinity receptor, BLT1, and a low-affinity receptor, BLT2. Both receptors mainly couple to pertussis toxin-sensitive Gi-like G proteins and induce cell migration...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yokomizo T

    更新日期:2015-02-01 00:00:00

  • Structure, interactions and effects on activity of the 5'-terminal region of human telomerase RNA.

    abstract::Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

    更新日期:2007-05-01 00:00:00

  • Reconstitution of Na+/H(+)-antiporter of bovine renal brush-border membrane into proteoliposomes and detection of a 110 kDa protein cross-reactive with antibodies against a human Na+/H(+)-antiporter partial peptide in antiport-active fractions after parti

    abstract::Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamashita T,Kawakita M

    更新日期:1992-02-01 00:00:00

  • Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems.

    abstract::We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

    更新日期:2008-12-01 00:00:00

  • Structural characterization of the gene for human histidine-rich glycoprotein, reinvestigation of the 5'-terminal region of cDNA and a search for the liver specific promoter in the gene.

    abstract::Genomic DNA libraries were screened for the human histidine-rich glycoprotein (HRG) gene and a sequence of 15,499 nucleotides was determined. The gene is composed of 7 exons and 6 introns, and all the exon-intron boundaries match the consensus GT/AG sequence for donor and acceptor splice sites. Each of cystatin-like d...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Wakabayashi S,Takahashi K,Koide T

    更新日期:1999-03-01 00:00:00

  • Characterization of human serum mannan-binding protein promoter.

    abstract::Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying the wide intra- and interracial variety in the MBP serum level, we have studied the transcriptional regulation of human MBP. Rapid amplification of cDNA end...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Naito H,Ikeda A,Hasegawa K,Oka S,Uemura K,Kawasaki N,Kawasaki T

    更新日期:1999-12-01 00:00:00

  • Further studies on the ionic strength-dependent proteolytic activation of protein kinase C in rat liver plasma membrane by endogenous trypsin-like protease.

    abstract::cAMP and Ca2(+)-independent histone kinase was generated from rat liver plasma membrane in an ionic strength-dependent manner by the action of an endogenous trypsin-like protease (Hashimoto, E. et al. (1986) FEBS Lett. 200, 63-66). In addition to the effect of ionic strength, this proteolytic activation of protein kin...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hashimoto E,Yamamura H

    更新日期:1989-12-01 00:00:00

  • Interaction with the Rev response element along an extended stem I duplex structure is required to complete human immunodeficiency virus type 1 rev-mediated trans-activation in vivo.

    abstract::Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kimura T,Ohyama A

    更新日期:1994-05-01 00:00:00

  • Effects of aging and denervation on the expression of uncoupling proteins in slow- and fast-twitch muscles of rats.

    abstract::We investigated the effects of aging and denervation on the gene expression of uncoupling proteins (UCPs) in slow-twitch soleus and fast-twitch gastrocnemius muscles. In a comparison between the control limbs of 6- and 24-month-old rats, the mRNA levels of UCP3, heart-type fatty acid binding protein (HFABP), and gluco...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kontani Y,Wang Z,Furuyama T,Sato Y,Mori N,Yamashita H

    更新日期:2002-08-01 00:00:00

  • Purification and specificity of a cell-wall-associated proteinase from Lactobacillus helveticus CP790.

    abstract::A cell-wall-associated proteinase from Lactobacillus helveticus CP790, grown in skim milk, was purified to homogeneity by DEAE ion exchange chromatography in the presence of EDTA. Its molecular weight was estimated to be 45,000 by SDS-PAGE and also by the recovery of proteinase activity only from this fraction of SDS-...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamamoto N,Akino A,Takano T

    更新日期:1993-11-01 00:00:00

  • Biosynthesis of isoprenoids in intact cells of Escherichia coli.

    abstract::Upon rehydration of lyophilized Escherichia coli cells with phosphate buffer containing [14C]isopentenyl pyrophosphate (IPP), 14C was incorporated into the cells. Radioactivity was found in ubiquinone-8, an unidentified precursor of ubiquinone-8, demethylmenaquinone-8 and phosphate esters of all-trans-octaprenol and c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujisaki S,Nishino T,Katsuki H

    更新日期:1986-04-01 00:00:00

  • Ca2+-sensitivity of actomyosin ATPase purified from Physarum polycephalum.

    abstract::A contractile protein closely resembling natural actomyosin (myosin B) of rabbit skeletal muscle was extracted from plasmodia of the slime mold, Physarum polycephalum, by protecting the SH-groups with beta-mercaptoethanol or dithiothreitol. Superprecipitation of the protein induced by Mg2+-ATP at low ionic strength wa...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kato T,Tonomura Y

    更新日期:1975-06-01 00:00:00

  • Anti-helix-loop-helix domain antibodies: discovery of autoantibodies that inhibit DNA binding activity of human centromere protein B (CENP-B).

    abstract::Centromere protein B (CENP-B) is one of the centromere DNA binding proteins constituting centromeric heterochromatin of human chromosomes. This protein was originally identified as the target antigen in autoimmune disease patients (often with scleroderma). In this study, we cloned a human CENP-B cDNA which was longer ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugimoto K,Muro Y,Himeno M

    更新日期:1992-04-01 00:00:00

  • Calcium regulation in chicken gizzard muscle and inosine triphosphate-induced superprecipitation of skeletal acto-gizzard myosin.

    abstract::Inosine triphosphate (ITP) does not serve as a substrate for myosin light-chain kinase from gizzard muscle. That is to say, myosin light-chain is not phosphorylated in ITP media. Nevertheless, at pH 6.8, 1 mM or 5 mM ITP induces superprecipitation of skeletal acto-gizzard myosin. The ITP-induced superprecipitation occ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Onishi H,Watanabe S

    更新日期:1979-08-01 00:00:00

  • Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5'-nucleotidase A (S5nA) found in Streptococcus iniae.

    abstract::Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Soh KY,Loh JMS,Proft T

    更新日期:2018-08-01 00:00:00

  • The amino-terminal hydrophobic region of the small subunit of calcium-activated neutral protease (CANP) is essential for its activation by phosphatidylinositol.

    abstract::Ca2+-Activated neutral protease (CANP), that consists of 80K and 30K subunits, is converted to a low-Ca2+-requiring form by autolysis in the presence of Ca2+. Phosphatidylinositol greatly reduces the Ca2+-requirement for the autolysis of native CANP. However, this effect was not observed for CANP with a trimmed 30K su...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Imajoh S,Kawasaki H,Suzuki K

    更新日期:1986-04-01 00:00:00

  • Duality of alpha-subunit of canine kidney sodium- and potassium-activated adenosinetriphophatase in electrophoresis.

    abstract::Sodium- and potassium-activated adenosinetriphosphatase (Na+, K+-ATPase) purified from dog kidney outer medulla was examined by polyacrylamide gel electrophoresis and by photoaffinity labeling with N-(ouabain)-N'-(2-nitro-4-azidophenyl)-ethylenediamine (NAP-ouabain). The large subunit band (alpha-band) split into two ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohta T,Kawamura M,Hasegawa T,Ishikura H,Nagano K

    更新日期:1982-01-01 00:00:00

  • Cloning of the RNA polymerase alpha subunit gene from Thermus thermophilus HB8 and characterization of the protein.

    abstract::The region containing the RNA polymerase alpha subunit (RNAPalpha) gene (rpoA) and the ribosomal protein genes of a thermophilic eubacterial strain, Thermus thermophilus (Tt) HB8, was cloned from a genomic DNA library by Southern hybridization. The gene order in this region is rpl36-rps13-rps11-rps4-rpoA-rpl17, which ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Wada T,Yamazaki T,Kuramitsu S,Kyogoku Y

    更新日期:1999-01-01 00:00:00