Cleavage specificity of cucumisin, a plant serine protease.


:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. The favorable synthetic substrates for cucumisin were Glt-Ala-Ala-Pro-Leu-pNA and Suc-Ala-Ala-Pro-Phe-pNA. The constant (kcat/Km) for Suc-Ala-Pro-Ala-pNA was found to be 30 times greater than that for Suc-Ala-Ala-Ala-pNA. The substrate specificity of cucumisin for oligopeptides and proteins was shown to be broad.


J Biochem


Journal of biochemistry


Uchikoba T,Yonezawa H,Kaneda M




Has Abstract


1995-05-01 00:00:00












  • Structure and function of the two heads of the myosin molecule. IV. Physiological functions of various reaction intermediates in myosin adenosinetriphosphatase, studied by the interaction between actomyosin and 8-bromoadenosine triphosphate.

    abstract::The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takenaka H,Ikehara M,Tonomura Y

    更新日期:1976-12-01 00:00:00

  • Interaction of native and SH-modified beta-amylase of soybean with cyclohexadextrin and maltose.

    abstract::Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mikami B,Nomura K,Morita Y

    更新日期:1983-07-01 00:00:00

  • Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig.

    abstract::In a previous study, we purified PAF-acetylhydrolase, which converts PAF to an inactive metabolite, lysoPAF, from peritoneal fluid of guinea pigs subjected to experimental endotoxin shock and found that this purified enzyme had similar biochemical properties to the plasma enzyme [Karasawa, K., Yato, M., Setaka, M., an...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Karasawa K,Kuge O,Kawasaki K,Nishijima M,Nakano Y,Tomita M,Yokoyama K,Setaka M,Nojima S

    更新日期:1996-10-01 00:00:00

  • Signal transduction and sporulation in Bacillus subtilis: heterologous phosphorylation of Spo0A, a sporulation initiation gene product.

    abstract::Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Asayama M,Kobayashi Y

    更新日期:1993-09-01 00:00:00

  • Oxygen metabolism of human colostral macrophages: comparison with monocytes and polymorphonuclear leukocytes.

    abstract::Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuda H,Takeshige K,Shibata Y,Minakami S

    更新日期:1984-05-01 00:00:00

  • Incorporation of bile acid of low concentration into model and biological membranes studied by 2H and 31P NMR.

    abstract::We have analyzed the manner of incorporation of bile acid into lipid bilayers and resultant perturbation of the bilayer structure with lower bile acid/lipid ratios relevant to the physiological conditions (approximately 1 mM) by 2H and 31P NMR methods, as an aid to understanding the possible role as an endogenous tumo...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saitŏ H,Sugimoto Y,Tabeta R,Suzuki S,Izumi G,Kodama M,Toyoshima S,Nagata C

    更新日期:1983-12-01 00:00:00

  • Purification and some properties of intracellular esterase from Pseudomonas fluorescens.

    abstract::A novel esterase was found in Pseudomonas fluorescens cells and purified to homogeneity as determined by polyacrylamide gel electrophoresis. The esterase was extracted from the cells by freeze-thawing and hypotonic treatment. Purification was achieved by ammonium sulfate precipitation, followed by successive chromatog...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakagawa A,Tsujita T,Okuda H

    更新日期:1984-04-01 00:00:00

  • Chemical modification of the most reactive thiol group of rabbit skeletal muscle phosphofructokinase, to reduce its affinity toward substrate ATP and activating monovalent cations.

    abstract::The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakajima Y,Nakamura K

    更新日期:1991-02-01 00:00:00

  • The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity.

    abstract::OsGEN-L has a 5'-flap endonuclease activity and plays an essential role in rice microspore development. The Class 4 RAD2/XPG family nucleases, including OsGEN-L, were recently found to have resolving activity for the Holliday junction (HJ), the intermediate of DNA strand recombination. In this study, we performed a de...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yang Y,Ishino S,Yamagami T,Kumamaru T,Satoh H,Ishino Y

    更新日期:2012-03-01 00:00:00

  • Conformational changes in inhibitory PAS domain protein associated with binding of HIF-1α and Bcl-xL in living cells.

    abstract::Inhibitory PAS domain protein (IPAS) is a dual function protein acting as a transcriptional repressor and as a pro-apoptotic protein. Simultaneous dual-color single-molecule imaging of EGFP-IPAS coexpressed with Mit-TagRFP-T in living HeLa cells revealed that fraction of EGFP-IPAS was arrested in the nucleus and on mi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kasai S,Kajimoto S,Ito Y,Saito T,Yasumoto KI,Tokunaga M,Sakata-Sogawa K,Fukumura H,Sogawa K

    更新日期:2017-03-01 00:00:00

  • Roles of nucleoside triphosphates in microtubule assembly.

    abstract::Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kobayashi T,Simizu T

    更新日期:1976-06-01 00:00:00

  • Solubilization of valine-sensitive acetohydroxy acid synthetase from Neurospora mitochondria.

    abstract::Acetohydroxy acid synthetase [EC] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kuwana H,Date M

    更新日期:1975-01-01 00:00:00

  • Two types of sarcoplasmic reticulum-orthophosphate interactions observed with dye probe.

    abstract::The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakamaru Y,Nomura K

    更新日期:1977-02-01 00:00:00

  • Feedback inhibition by methionine and S-adenosylmethionine, and desensitization of homoserine O-acetyltransferase in Brevibacterium flavum.

    abstract::Homoserine O-acetyltransferase [EC] partially purified from Brevibacterium flavum was found to be specifically inhibited by the metabolic end products methionine and S-adenosylmethionine only when the enzymatic reaction was performed in the presence of cysteine or dithiothreitol, or after the preincubation of...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shiio I,Ozaki H

    更新日期:1981-05-01 00:00:00

  • Molecular cloning and sequence analysis of full-length cDNA for rabbit liver NADPH-cytochrome P-450 reductase mRNA.

    abstract::The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Katagiri M,Murakami H,Yabusaki Y,Sugiyama T,Okamoto M,Yamano T,Ohkawa H

    更新日期:1986-10-01 00:00:00

  • Interaction of thiolsubtilisin with Streptomyces subtilisin inhibitor, SSI.

    abstract::Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

    更新日期:1977-07-01 00:00:00

  • Blocking effect of trypsin associated with ovomucoid on the antibody binding to ovomucoid.

    abstract::Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsuda T,Watanabe K,Nakamura R

    更新日期:1983-07-01 00:00:00

  • Genomic structure of human midkine (MK), a retinoic acid-responsive growth/differentiation factor.

    abstract::Midkine (MK) is a product of a retinoic acid-responsive gene and a new heparin-binding growth/differentiation factor. The coding sequence of human MK was located on 1.5 kb DNA segment. The structure of the cloned human MK gene was determined and compared with that of the mouse gene. The coding sequence was divided int...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Uehara K,Matsubara S,Kadomatsu K,Tsutsui J,Muramatsu T

    更新日期:1992-05-01 00:00:00

  • Preparation and chemical characterization of the three chains of the major hemoglobin of the sea snake, Pelamis platurus.

    abstract::One of the 2 main hemoglobins of the sea snake Pelamis platurus, (the Yellow-bellied sea snake) comprising about 70% of the total hemoglobin, was separated by DEAE-Sephadex column chromatography. From results of gel filtration and iron content determination, both intact sea snake hemoglobin, and the isolated major hem...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Liu CS

    更新日期:1975-07-01 00:00:00

  • Purification and characterization of 7 beta-hydroxysteroid dehydrogenase from Ruminococcus sp. of human intestine.

    abstract::7 beta-Hydroxysteroid dehydrogenase (7 beta-HSD) was produced by Ruminococcus sp. PO1-3 obtained from among human intestinal bacteria. The enzyme was purified from a crude extract by ammonium sulfate fractionation, and Butyl-Toyopearl 650M, Sephadex G-150, Matrex Red A and Octyl-Sepharose chromatographies. The purifie...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Akao T,Akao T,Kobashi K

    更新日期:1987-09-01 00:00:00

  • Multiplication-stimulating activity (MSA) and cartilage-derived factor (CDF): biological actions in cultured chondrocytes.

    abstract::Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

    更新日期:1981-11-01 00:00:00

  • Identification of novel members of the Xenopus Ca2+ -dependent lectin family and analysis of their gene expression during tail regeneration and development.

    abstract::We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo T

    更新日期:2007-04-01 00:00:00

  • A large-scale preparation and some physicochemical properties of recA protein.

    abstract::Pure recA protein was easily obtained from Escherichia coli harboring plasmid pTM-2 which carried the recA gene by two chromatographic steps on phosphocellulose and DEAE-cellulose. RecA protein was stable in the pH range of 6 to 9 at 25 degrees C. RecA protein was found to aggregate highly under these conditions. Lowe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kuramitsu S,Hamaguchi K,Ogawa T,Ogawa H

    更新日期:1981-10-01 00:00:00

  • Biochemical characterization of galloyl pedunculagin (ellagitannin) as a selective inhibitor of the beta-regulatory subunit of A-kinase in vitro.

    abstract::The inhibitory effects of galloyl pedunculagin (GP) and eugeniin on the phosphorylation of histone H2B by cAMP-dependent protein kinase (A-kinase) and autophosphorylation of its beta-regulatory subunit (A-kinase beta) were examined in vitro. It was found that (i) GP (ID(50) = approx. 50 nM) effectively inhibits the ac...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kosuge S,Maekawa T,Saito C,Tanaka T,Kouno I,Ohtsuki K

    更新日期:2001-03-01 00:00:00

  • The role of nuclear serine proteases in the degradation of newly synthesized histones and ribosomal proteins.

    abstract::To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsurugi K,Oyanagi M,Ogata K

    更新日期:1983-05-01 00:00:00

  • Mitochondrial porin can be translocated across both endoplasmic reticulum and mitochondrial membranes.

    abstract::Mitochondrial porin is a major integral membrane protein of the outer membrane. To assess the stop-transfer sequence in the yeast porin molecule (P), we constructed the following chimeric proteins. (i) The signal sequence of interleukin 2, a secretory protein, was fused to the amino-terminus of porin (SP). (ii) The ma...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sakaguchi M,Hachiya N,Mihara K,Omura T

    更新日期:1992-08-01 00:00:00

  • Calcium regulation in chicken gizzard muscle and inosine triphosphate-induced superprecipitation of skeletal acto-gizzard myosin.

    abstract::Inosine triphosphate (ITP) does not serve as a substrate for myosin light-chain kinase from gizzard muscle. That is to say, myosin light-chain is not phosphorylated in ITP media. Nevertheless, at pH 6.8, 1 mM or 5 mM ITP induces superprecipitation of skeletal acto-gizzard myosin. The ITP-induced superprecipitation occ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Onishi H,Watanabe S

    更新日期:1979-08-01 00:00:00

  • Circadian rhythms in digestive enzymes in the small intestine of rats. I. Patterns of the rhythms in various regions of the small intestine.

    abstract::The activities of the digestive enzymes, maltase [EC], sucrase [EC], trehalase [EC], Leucine aminopeptidase [EC], and alkaline phosphatase [EC] were measured in various regions of the small intestine of rats. The activities of all these enzymes were much higher in the jejunu...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saito M,Murakami E,Nishida T,Fujisawa Y,Suda M

    更新日期:1975-09-01 00:00:00

  • Essential roles of carbohydrate signals in development, immune response and tissue functions, as revealed by gene targeting.

    abstract::Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Muramatsu T

    更新日期:2000-02-01 00:00:00

  • Direct participation of potassium ion in the catalysis of coenzyme B(12)-dependent diol dehydratase.

    abstract::The direct ion-dipolar interactions between potassium ion (K(+)) and the two hydroxyl groups of the substrate are the most striking feature of the crystal structure of coenzyme B(12)-dependent diol dehydratase. We carried out density-functional-theory computations to determine whether K(+) can assist the 1,2-shift of ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Toraya T,Yoshizawa K,Eda M,Yamabe T

    更新日期:1999-10-01 00:00:00