Disulfide bonds of purothionine, a lethal toxin for yeasts.

abstract：:G-actin freed from exogenous ATP was added to the pieces of isolated acrosomal actin bundles from horseshoe crab sperm to form filaments as reported earlier (Tilney, L.G., Bonder, E.M., & DeRosier, D.J. (1981) J. Cell Biol. 90, 485-494). The growth of a filament was far more rapid at one end (the preferred end) than t...

journal_title：Journal of biochemistry

authors： Mabuchi I

更新日期：1983-10-01 00:00:00

abstract：:High mobility group box protein 1, HMGB1, is a major nonhistone chromatin component in higher eukaryotic cells. HMGB1 is thought to be involved in the processes of global nuclear events such as transcription, recombination and repair, but the mechanism of these processes is unclear. Here, we show a concrete example of...

journal_title：Journal of biochemistry

authors： Yamada M,Ueda T,Sato K,Yoshida M

更新日期：2004-01-01 00:00:00

abstract：:When meso-tetrakis(3-N-methylpyridiniumyl)porphyrin (m-TMPyP) formed a complex with poly[d(A-T)(2)], an intense bisignate excitonic CD in the Soret absorption region was observed. The excitonic CD of the m-TMPyP-poly[d(A-T)(2)] complex is unique in that no other combination of the related porphyrin, namely, meso-tetra...

journal_title：Journal of biochemistry

authors： Lee YA,Lee S,Lee HM,Lee CS,Kim SK

更新日期：2003-03-01 00:00:00

abstract：:Identification of single-stranded regions in Torulopsis utilis 5S RNA was attempted by the use of Nuclease S1, a single-strand specific endonuclease. When T. utilis 5S RNA was subjected to prolonged incubation with Nuclease S1, about 50% of the substrate 5S RNA remained as large oligonucleotide "cores." Such Nuclease...

journal_title：Journal of biochemistry

authors： Nishikawa K,Takemura S

更新日期：1977-04-01 00:00:00

abstract：:Low molecular weight RNAs were isolated from nuclei of the cellular slime mold Dictyostelium discoideum AX-3. Analysis of the RNAs by polyacrylamide gel electrophoresis showed that the vegetative cell nuclei contained, besides tRNA (Dd1), 5S RNA (Dd4), and 5.8S RNA (Dd7), at least 7 small RNA species (Dd3, Dd5, Dd6, D...

journal_title：Journal of biochemistry

authors： Takeishi K,Kaneda S

更新日期：1981-08-01 00:00:00

abstract：:A new sulfated polysaccharide was isolated from the culture supernatant of a strain of Arthrobacter sp. The polysaccharide purified with quaternary ammonium salts consists of D-galactose, D-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and LL-diaminopimelic acid in a molar r...

journal_title：Journal of biochemistry

authors： Inoue K,Korenaga H,Kadoya S

更新日期：1982-12-01 00:00:00

abstract：:In the crude extract of matured eggs of the silkworm (Bombyx mori), proteolytic activity was detected only in the acidic pH region with maximal activity at pH 3.5, and this activity was maintained throughout the development. No appreciable activity was observed in the neutral to alkaline pH region either in matured eg...

journal_title：Journal of biochemistry

authors： Kageyama T,Takahashi SY,Takahashi K

更新日期：1981-09-01 00:00:00

abstract：:A comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. The ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, b...

journal_title：Journal of biochemistry

authors： Yamane A,Hirabayashi T

更新日期：1985-05-01 00:00:00

abstract：:Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1...

journal_title：Journal of biochemistry

authors： Fukumori F,Takeuchi N,Hagiwara T,Ohbayashi H,Endo T,Kochibe N,Nagata Y,Kobata A

更新日期：1990-02-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:In sea urchin embryos, primary mesenchyme cells, descendants from micromeres produced at the 16-cell stage, form spicules or CaCO3 deposits in their skeletal vacuoles, at the post-gastrula stage. Micromeres isolated at the 16-cell stage also differentiate into spicule-forming cells during their culture at the same tim...

journal_title：Journal of biochemistry

authors： Mitsunaga K,Fujino Y,Yasumasu I

更新日期：1986-12-01 00:00:00

abstract：:2-Amino-4-methylphenol was converted to a brownish yellow material by the lysates of human erythrocytes or purified human hemoglobin. The reaction proceeded oxidatively, coupled with the oxidation of hemoglobin. The major component of the brownish yellow material produced by oxidative condensation of 2-amino-4-methylp...

journal_title：Journal of biochemistry

authors： Tomoda A,Arisawa M,Koshimura S

更新日期：1991-12-01 00:00:00

abstract：:Photoaffinity labeling with [3H]cytochalasin B detects two D-glucose-sensitive proteins in the chicken embryo fibroblast (CEF) plasma membrane, which accumulate under conditions of glucose starvation and are probably involved in the glucose transport system (Pessin, J.E., et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, ...

journal_title：Journal of biochemistry

authors： Ishii T,Tillotson LG,Isselbacher KJ

更新日期：1986-01-01 00:00:00

abstract：:Amyloid formation is the hallmark of many diseases. The propensity of a protein to aggregate depends on a number of biological factors like the presence of sulphated polysaccharides termed as glycosaminoglycans (GAGs). Here we assessed whether the polymeric nature of GAGs is responsible for their protein aggregation-p...

journal_title：Journal of biochemistry

authors： Motamedi-Shad N,Monsellier E,Chiti F

更新日期：2009-12-01 00:00:00

abstract：:The effects of unsaturated fatty acids on the activities of peroxisomal enzymes of Tetrahymena pyriformis were investigated. When saturated fatty acids and the corresponding unsaturated fatty acids (C18) were added to the culture medium at 0.05%, the activities of peroxisomal enzymes [fatty acyl-CoA oxidase (FAO), car...

journal_title：Journal of biochemistry

authors： Gotoh K,Takei M,Watanabe T,Suga T

更新日期：1986-10-01 00:00:00

abstract：:The DNA fragment containing the phoS gene, a regulatory gene for alkaline phosphatase, has been isolated from Escherichia coli K-12 chromosomal DNA by cutting off the DNA with Hind III restriction enzyme and by cloning the gene with plasmid vector pTP 4 which was constructed in this study. The isolated fragment was of...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Tsuda K

更新日期：1982-09-01 00:00:00

abstract：:Spontaneous insertion of membrane proteins into liposomes formed from Escherichia coli polar phospholipids is blocked by diacylglycerol (DAG) at a physiological level. We found that cholesterol also blocks this spontaneous insertion, although a much larger amount is necessary for sufficient blockage. Reversely, sphing...

journal_title：Journal of biochemistry

authors： Nakamura S,Suzuki S,Saito H,Nishiyama KI

更新日期：2018-04-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology a...

journal_title：Journal of biochemistry

authors： Lu JF,Pokharel D,Padula MP,Bebawy M

更新日期：2016-11-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide disc gel electrophoresis. The molecular weights of these enzymes w...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1977-03-01 00:00:00

abstract：:Thymidine kinase [ATP: thymidine 5'-phosphotransferase, EC 2.7.1.21] has been purified more than 3,500 fold from microplasmodia of Physarum polycephalum. Properties of the enzyme were determined on preparations purified 1,400 fold. Thymidine was transformed to dTMP while a stoichiometric quantity of ATP was transforme...

journal_title：Journal of biochemistry

authors： Chraibi R,Wright M

更新日期：1983-02-01 00:00:00

abstract：:An enzyme activity specifically active on nitrous acid-treated DNA was found in an extract of Escherichia coli. The enzyme acts on both double- and single-stranded DNAs, treated with nitrous acid, in the presence of EDTA, although the former DNA is a better substrate. Evidence is presented that nitrous acid- and bisul...

journal_title：Journal of biochemistry

authors： Oeda K,Shimizu K,Sekiguchi M

更新日期：1978-11-01 00:00:00

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its cytosolically exposed domain, is expressed in Saccharomyces cerevisiae. Using various KAR2 promoter constructs in front of ...

journal_title：Journal of biochemistry

authors： Zimmer T,Ogura A,Ohta A,Takagi M

更新日期：1999-12-01 00:00:00

abstract：:Recombinant horse L-apoferritin and its mutants were used to compare the reactivities of two different cysteinyl residues with 7-fluoro-4-sulfamoyl-2,1,3-benzoxadiazole (ABD-F), p-chloromercuribenzoic acid (PCMB), N-(9-acridinyl)maleimide (NAM), and 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl (NEM-TEMPO). ABD-F s...

journal_title：Journal of biochemistry

authors： Takeda S,Yamaki M,Ebina S,Nagayama K

更新日期：1995-02-01 00:00:00

abstract：:The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the s...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：1984-06-01 00:00:00