The terminal bases of ribonucleic acid from Caulobacter RNA phage phiCp2.

abstract：:We studied the mechanism of formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study. The hydroxyl radical is also generated through Fenton t...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Tero-Kubota S,Yamamoto Y,Kato M,Nakano M,Yagi K,Ogino K

更新日期：2003-12-01 00:00:00

abstract：:The active site of thermolysin is composed of one zinc ion and five polypeptide regions [N-terminal sheet (Asn112-Trp115), alpha-helix 1 (Val139-Thr149), C-terminal loop 1 (Asp150-Gly162), alpha-helix 2 (Ala163-Val176) and C-terminal loop 2 (Gln225-Ser234)]. To explore their catalytic roles, we introduced single amino...

journal_title：Journal of biochemistry

authors： Kusano M,Yasukawa K,Inouye K

更新日期：2009-01-01 00:00:00

abstract：:Effects of rice bran agglutinin (RBA) on human monoblastic leukemia U937 cells were examined in comparison with those of wheat germ agglutinin (WGA) and Viscum album agglutinin (VAA). These lectins inhibit cell growth, and several lines of evidence indicate that the growth inhibition is caused by the induction of apop...

journal_title：Journal of biochemistry

authors： Miyoshi N,Koyama Y,Katsuno Y,Hayakawa S,Mita T,Ohta T,Kaji K,Isemura M

更新日期：2001-12-01 00:00:00

abstract：:GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

更新日期：1983-03-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:The effect of crosslinking of F-actin with glutaraldehyde on the contractility of muscle ghost fiber containing reconstituted thin filament (i.e. F-actin-tropomyosin-troponin complex) and irrigated with myosin was investigated. The results show that: (i) crosslinking inhibited development of isometric tension and shor...

journal_title：Journal of biochemistry

authors： Próchniewicz-Nakayama E,Yanagida T

更新日期：1982-10-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:We have examined critically whether or not new and old histones mix in the octameric units of nucleosomes during chromatin replication. MH-134SC cells were density-labeled by culturing with amino acid mixtures enriched with dense isotopes 2H, 13C, and 15N. Mononucleosomes obtained from labeled cells were fractionated ...

journal_title：Journal of biochemistry

authors： Yamasu K,Senshu T

更新日期：1987-04-01 00:00:00

abstract：:The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In ...

journal_title：Journal of biochemistry

authors： Takiya S,Saito S,Yokoyama T,Matsumoto D,Aizawa T,Kamiya M,Demura M,Kawano K

更新日期：2009-07-01 00:00:00

abstract：:To investigate the structure-activity relationships, various biological activities, including pyrogenicity, lethal toxicity, elicitation of Shwartzman reaction, mitogenicity and tumor necrosis factor (TNF)-inducing activity, were compared among natural and synthetic lipid A's differing in fatty acid composition. In al...

journal_title：Journal of biochemistry

authors： Kanegasaki S,Tanamoto K,Yasuda T,Homma JY,Matsuura M,Nakatsuka M,Kumazawa Y,Yamamoto A,Shiba T,Kusumoto S

更新日期：1986-04-01 00:00:00

abstract：:The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-a-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromot...

journal_title：Journal of biochemistry

authors： Matsuda Y,Moriya H,Moriwaki C,Fujimoto Y,Matsuda M

更新日期：1976-06-01 00:00:00

abstract：:Guinea pig alveolar macrophages (A-M0) were studied with respect ot the phagocytic metabolism: the respiratory burst and its KCN sensitivity, the ability to generate H2O2, and myeloperoxidase (MPO) activity. Only fifty percent of the O2 uptake by A-M0 samples during phagocytosis was inhibited by the addition of 1 mK K...

journal_title：Journal of biochemistry

authors： Kaneda M,Kakinuma K,Yamaguchi T,Shimada K

更新日期：1980-10-01 00:00:00

abstract：:In this work we have studied the equilibrium and kinetic stability of a hyperthermophilic protein, O(6)-methylguanine-DNA methyltransferase (Tk-MGMT), and its mesophilic counterpart AdaC, in various chemical solutions. In an unfolding experiment using guanidine hydrochloride (GdnHCl), the unfolding free-energy change ...

journal_title：Journal of biochemistry

authors： Nishikori S,Shiraki K,Okanojo M,Imanaka T,Takagi M

更新日期：2004-10-01 00:00:00

abstract：:Photoaffinity labeling with [3H]cytochalasin B detects two D-glucose-sensitive proteins in the chicken embryo fibroblast (CEF) plasma membrane, which accumulate under conditions of glucose starvation and are probably involved in the glucose transport system (Pessin, J.E., et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, ...

journal_title：Journal of biochemistry

authors： Ishii T,Tillotson LG,Isselbacher KJ

更新日期：1986-01-01 00:00:00

abstract：:The protein level of the GTS1 gene product (Gts1p) fluctuated during the oscillation of energy metabolism in continuous culture of the yeast Saccharomyces cerevisiae. Here, we found that the GTS1 mRNA level oscillated with the same periodicity as the metabolic oscillation, suggesting that the expression of GTS1 was re...

journal_title：Journal of biochemistry

authors： Tonozuka H,Wang J,Mitsui K,Saito T,Hamada Y,Tsurugi K

更新日期：2001-11-01 00:00:00

abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

journal_title：Journal of biochemistry

authors： Takahashi K,Shimidzu M,Shindo H,Kawamoto T,Nishi M,Matsumoto U,Taniguchi S

更新日期：1987-05-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...

journal_title：Journal of biochemistry

authors： Miwa T,Akaboshi E,Matsubara K

更新日期：1983-08-01 00:00:00

abstract：:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...

journal_title：Journal of biochemistry

authors： Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka T

更新日期：2003-08-01 00:00:00

abstract：:Previously, we reported that proteasomes (large multi-protease complexes) are present in a latent state in a variety of eukaryotic cells, and can be activated by treatment with various compounds such as sodium dodecyl sulfate (SDS) or poly-lysine (Tanaka et al. (1988) J. Biol. Chem. 263, 16209-16217). In the present s...

journal_title：Journal of biochemistry

authors： Tanaka K,Yoshimura T,Ichihara A

更新日期：1989-09-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00

abstract：:S-RNase is associated with the gametophytic self-incompatibility of flowering plants in Solanaceae and, on the basis of sequence homology, belongs to the RNase T2 family. To identify the active site residues in S-RNase, Nicotiana alata S6-RNase was studied by chemical modification. S6-RNase was inactivated with iodoac...

journal_title：Journal of biochemistry

authors： Ishimizu T,Miyagi M,Norioka S,Liu YH,Clarke AE,Sakiyama F

更新日期：1995-11-01 00:00:00

abstract：:alpha and beta chains from adult hemoglobin of the slender loris (Loris tardigradus) were isolated by Amberlite CG-50 column chromatography. After S-aminoethylation, both chains were digested with trypsin and the amino acid sequences of the tryptic peptides obtained were analyzed. Further, the order of these tryptic p...

journal_title：Journal of biochemistry

authors： Maita T,Goodman M,Matsuda G

更新日期：1978-08-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:Carboxyl groups of native human fibrinogen were modified with glycine methyl ester and 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. It seemed likely that the modification occurred stepwise. Approximately 26% of the carboxyl groups of fibrinogen was modified finally. The modified fibrinogen had no interaction with cat...

journal_title：Journal of biochemistry

authors： Kurioka S,Inoue F,Matsuda M

更新日期：1975-11-01 00:00:00

abstract：:The position in the acyl phosphate linkage of the phosphorylated intermediate of (Na+, K+)-ATPase that is cleaved by N-methylhydroxylamine was compared with that of the model compound acetylphosphate. The products of the cleavage of the phosphoenzyme by methylhydroxylamine were the active enzyme and a N-P compound, no...

journal_title：Journal of biochemistry

authors： Ushimaru M,Shinohara Y,Fukushima Y

更新日期：1997-09-01 00:00:00

abstract：:Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related...

journal_title：Journal of biochemistry

authors： Kato S,Tsuji M,Nakanishi Y,Suzuki S

更新日期：1980-03-01 00:00:00

abstract：:Aldehyde reductases from several mammalian and avian tissues transferred the pro-4R hydrogen of NADPH to the substrate, whereas the stereospecificity of carbonyl reductases was not uniform being correlated with the ability to catalyze the oxidoreduction of hydroxysteroids. ...

journal_title：Journal of biochemistry

authors： Nakayama T,Hara A,Kariya K,Sawada H

更新日期：1985-10-01 00:00:00

abstract：:An open reading frame of 828 base pairs was found in the CHO1 gene region of Saccharomyces cerevisiae by nucleotide sequencing analysis. Its enhanced expression with the aid of the PHO5 regulatory sequence resulted in an overproduction of a protein with a molecular weight of approximately 30,000, which in turn was con...

journal_title：Journal of biochemistry

authors： Kiyono K,Miura K,Kushima Y,Hikiji T,Fukushima M,Shibuya I,Ohta A

更新日期：1987-11-01 00:00:00