Abstract：
:The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin underwent irreversible cleavage forming unidentified radicals and cob(II)alamin that resisted oxidation even in the presence of oxygen. Two moles of 5'-deoxyadenosine per mol of enzyme was formed as an inactivation product from the coenzyme adenosyl group. Inactivated holoenzymes underwent reactivation by diol dehydratase-reactivating factor in the presence of ATP, Mg(2+) and adenosylcobalamin. It was thus concluded that these substrate analogues served as mechanism-based inactivators or pseudosubstrates, and that the coenzyme was damaged in the inactivation, whereas apoenzyme was not damaged. In the inactivation by 3-unsaturated 1,2-diols, product radicals stabilized by neighbouring unsaturated bonds might be unable to back-abstract the hydrogen atom from 5'-deoxyadenosine and then converted to unidentified products. In the inactivation by thioglycerol, a product radical may be lost by the elimination of sulphydryl group producing acrolein and unidentified sulphur compound(s). H(2)S or sulphide ion was not formed. The loss or stabilization of product radicals would result in the inactivation of holoenzyme, because the regeneration of the coenzyme becomes impossible.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori Kdoi
10.1093/jb/mvn086subject
Has Abstractpub_date
2008-10-01 00:00:00pages
437-46issue
4eissn
0021-924Xissn
1756-2651pii
mvn086journal_volume
144pub_type
杂志文章abstract：:In vitro activation of human reovirus transcriptase by alpha-chymotrypsin digestion of viral outer shell proteins was completely dependent on the ionic size of the monovalent cation in the medium. Cations with nonhydrated ionic radii larger than 1.3 A showed full potency of activation of chymotrypsin digestion, and pr...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134657
更新日期：1984-03-01 00:00:00
abstract：:Spontaneous insertion of membrane proteins into liposomes formed from Escherichia coli polar phospholipids is blocked by diacylglycerol (DAG) at a physiological level. We found that cholesterol also blocks this spontaneous insertion, although a much larger amount is necessary for sufficient blockage. Reversely, sphing...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvx083
更新日期：2018-04-01 00:00:00
abstract：:Neutral alpha-glucosidase was partially purified from granular fractions isolated from guinea pig polymorphonuclear leukocytes (PMNL). The native enzyme had a high molecular weight, about 417,000, with a subunit of 43,000. The purified enzyme hydrolysed 4-methylumbelliferyl alpha-glucoside and maltose, but not isomalt...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122914
更新日期：1989-10-01 00:00:00
abstract：:The present study demonstrates that the functional counter-receptors for E-selectin at the cell surface of Colo201 human colon cancer cells are localized in detergent-insoluble membrane microdomains (DIM). Following isolation of counter-receptors from whole cell lysates using E-selectin-coupled magnetic beads followed...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm107
更新日期：2007-07-01 00:00:00
abstract：:The modes of actions of 1-farnesylpyridinium (FPy) on yeast cell growth were investigated on the basis of its effects on cell cycle progression, morphogenesis and the related events for construction of cell wall architecture in Schizosacchromyces pombe. FPy predominantly inhibited the growth of the yeast cells after v...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvj218
更新日期：2006-12-01 00:00:00
abstract：:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122728
更新日期：1989-05-01 00:00:00
abstract：:The binding properties of dynein arms to the A- and B-tubules of outer doublets of cilia from Tetrahymena pyriformis were examined, with the following results: 1. When 30s dynein purified from Tetrahymena cilia was added to doublets deficient in dynein arms, it bound to both A- and B-tubules almost equally and formed ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132256
更新日期：1978-12-01 00:00:00
abstract：:ATPase of 14S dynein, extracted from spermatozoa of the sea urchin, Hemicentrotus pulcherrimus, and partially purified by sucrose density gradient centrifugation, was inhibited non-competitively by palmitoyl CoA at concentrations higher than 20 microns, and was stimulated at concentrations between 2 microns and 10 mic...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a133951
更新日期：1982-08-01 00:00:00
abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022186
更新日期：1998-10-01 00:00:00
abstract：:By analyzing antibody heterogeneity during the primary immune response to bacterial a-amylase (B alpha A) in high-responder F1 hybrid mice between C57BL/6 (B6) and C3H/He (C3) mice with the use of isoelectric focusing (IEF), it was shown that the maturation of the primary IgG antibody response consisted of at least tw...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134816
更新日期：1984-07-01 00:00:00
abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a121975
更新日期：1987-05-01 00:00:00
abstract：:Reports vary on the role of growth hormone (GH) in adipocyte differentiation. In this study, we showed that GH exerted dual effects depending on the stage of differentiation, using a serum-free culture of 3T3-L1 preadipocytes. GH promoted the differentiation when added to the medium during differentiation-inducing tre...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a003301
更新日期：2002-12-01 00:00:00
abstract：:Several maleimide derivatives of potential usefulness as conformational probes were tested for reactivity toward SH groups of Ca2+, Mg2+-ATPase of sarcoplasmic reticulum. These include three fluorescent labels, N-(1-anilinonaphthyl-4)maleimide (ANM), N-(p-(2-benzimidazolyl)phenyl)maleimide (BIPM), and N-(7-dimethylami...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134406
更新日期：1983-09-01 00:00:00
abstract：:Disulfide bond interchange has been pointed out as a considerable problem in preparing recombinant proteins from Escherichia coli cells. This has been reported in the system of reducing denaturation followed by a refolding process, where incorrectly folded molecules are sometimes produced. As the possibility of disulf...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122419
更新日期：1988-07-01 00:00:00
abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm068
更新日期：2007-05-01 00:00:00
abstract：:We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021795
更新日期：1997-09-01 00:00:00
abstract：:Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134333
更新日期：1983-07-01 00:00:00
abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132213
更新日期：1978-10-01 00:00:00
abstract：:Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dic...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134053
更新日期：1982-10-01 00:00:00
abstract：:A comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. The ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, b...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a135196
更新日期：1985-05-01 00:00:00
abstract：:Monoclonal antibody 5E5 recognized an intranuclear antigen of neurons in the rat. We isolated 5E5cDNA and determined its nucleotide and deduced amino acid sequences. The 5E5cDNA had an open reading frame of 825 amino acids and its amino acid sequence showed no significant homology to any protein or to any DNA binding ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124866
更新日期：1995-07-01 00:00:00
abstract：:To identify functionally essential sequences and residues of CTLA-2alpha, in vitro mutagenesis was carried out. The coefficient of inhibition (K(i)) was determined towards rabbit cathepsin L using Z-Phe-Arg-MCA as the substrate. Recombinant CTLA-2alpha inhibited the enzyme potently (K(i) = 15 nM). A truncated mutant, ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvp188
更新日期：2010-03-01 00:00:00
abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a003086
更新日期：2002-02-01 00:00:00
abstract：:Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin is a Ca2+-dependent regulator of muscle contraction. Both proteins are widely distributed in protostomes, while in deuterostomes, their distribution is limited; namely, presence of paramyosin and absence of troponin are commo...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvw047
更新日期：2016-12-01 00:00:00
abstract：:1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels o...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvp159
更新日期：2010-02-01 00:00:00
abstract：:We can detect phospholipase A activity in non-hemolyzed sheep erythrocytes, using dilauroylglycerophosphocholine as an exogenous substrate. Only substrates such as dilauroylglycerophosphocholine, which can be incorporated into membranes, could be hydrolyzed by the enzyme, egg phosphatidylcholine being only slightly se...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134426
更新日期：1983-09-01 00:00:00
abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122128
更新日期：1987-10-01 00:00:00
abstract：:Mitochondria are dynamic organelles that frequently divide and fuse together, resulting in the formation of intracellular tubular networks. In yeast and mammals, several factors including Drp1/Dnm1 and Mfn/Fzo1 are known to regulate mitochondrial morphology by controlling membrane fission or fusion. Here, we report th...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm245
更新日期：2008-04-01 00:00:00
abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132772
更新日期：1980-02-01 00:00:00
