Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro.

abstract：:Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

journal_title：Journal of biochemistry

authors： Sato S,Imamura S,Ozeki Y,Kawaguchi A

更新日期：1992-01-01 00:00:00

abstract：:Fluorescence titration curves of 2-[4'-iodoacetamido)anilino)naphthalene-6-sulfonic acid-labeled troponin (IAANS-labeled Tn) and troponin-1-anilinonaphthalene-8-sulfonic acid (Tn-ANS) complex indicated that the fluorescent moiety, IAANS or ANS, detects conformational change of troponin I (TnI) or Tn due to the Ca2+ bi...

journal_title：Journal of biochemistry

authors： Nishio T,Iio T

更新日期：1983-09-01 00:00:00

abstract：:The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...

journal_title：Journal of biochemistry

authors： Nakanishi N,Hasegawa H,Akino M,Yamada S

更新日期：1986-03-01 00:00:00

abstract：:The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the s...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：1984-06-01 00:00:00

abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...

journal_title：Journal of biochemistry

authors： Yohda M,Yoshida M

更新日期：1987-10-01 00:00:00

abstract：:Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

journal_title：Journal of biochemistry

authors： Nakanaga K,Hama K,Aoki J

更新日期：2010-07-01 00:00:00

abstract：:We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 m...

journal_title：Journal of biochemistry

authors： Nagino K,Nomura O,Takii Y,Myomoto A,Ichikawa M,Nakamura F,Higasa M,Akiyama H,Nobumasa H,Shiojima S,Tsujimoto G

更新日期：2006-04-01 00:00:00

abstract：:The interaction between pullulanase from Klebsiella pneumoniae and alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was examined by means of inhibition studies of the enzyme activity, UV difference spectroscopy, and flow calorimetry. All the abov...

journal_title：Journal of biochemistry

authors： Iwamoto H,Ohmori M,Ohno M,Hirose J,Hiromi K,Fukada H,Takahashi K,Hashimoto H,Sakai S

更新日期：1993-01-01 00:00:00

abstract：:RPE65 is a membrane-associated retinoid isomerase involved in the visual cycle responsible for sustaining vision. Many mutations in the human RPE65 gene are associated with distinct forms of retinal degenerative diseases. The pathogenic mechanisms for most of these mutations remain poorly understood. Here, we show tha...

journal_title：Journal of biochemistry

authors： Li S,Hu J,Jin RJ,Aiyar A,Jacobson SG,Bok D,Jin M

更新日期：2015-08-01 00:00:00

abstract：:Rat and mouse cDNAs for Zn-alpha 2-glycoprotein (Zn alpha 2gp) were isolated from liver libraries (lambda gt11) and compared with the human one. The lengths of cDNA inserts analyzed were 1,233 and 1,273 nucleotides for rat and mouse, respectively. The deduced amino acid sequences suggested that rat and mouse Zn alpha ...

journal_title：Journal of biochemistry

authors： Ueyama H,Naitoh H,Ohkubo I

更新日期：1994-09-01 00:00:00

abstract：:Mitochondrial processing peptidase (MPP), a metalloendopeptidase consisting of alpha and beta subunits, specifically recognizes a large variety of mitochondrial precursor proteins and cleaves off the N-terminal basic matrix targeting signals (MTS). Basic residues in MTS and acidic sites in MPP are required for effecti...

journal_title：Journal of biochemistry

authors： Kitada S,Ito A

更新日期：2001-01-01 00:00:00

abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...

journal_title：Journal of biochemistry

authors： Yamamoto K,Honjo R,Sekine T

更新日期：1980-01-01 00:00:00

abstract：:The DNA chain elongation mechanisms of mouse DNA polymerases alpha and beta have been analyzed by using denatured DNA with a (dT)n block at the 3'-end as a template in combination with RNA ((rA)12-20)primer. The (rA)12-20-primed DNA product synthesized by DNA polymerase alpha was 3-5 s in size even after prolonged rea...

journal_title：Journal of biochemistry

authors： Matsukage A,Nishizawa M,Takahashi T,Hozumi T

更新日期：1980-12-01 00:00:00

abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...

journal_title：Journal of biochemistry

authors： Sato M,Sato-Miyamoto Y,Kameyama K,Ishikawa N,Imai M,Ito Y,Takagi T

更新日期：1995-12-01 00:00:00

abstract：:To identify the residues modified by radioactive N6-p-bromoacetaminobenzyladenosine-5'-phosphate in phosphorylase b, the peptic peptides of the modified enzyme were purified. Two radioactive peptides were isolated, each of which had a cysteine residue modified. The cysteine residue involved in the AMP site was determi...

journal_title：Journal of biochemistry

authors： Kawashima S,Eguchi C,Imahori K

更新日期：1978-12-01 00:00:00

abstract：:We have previously purified two D-3-hydroxyacyl-CoA dehydratase preparations from human liver. One preparation contained a 77-kDa polypeptide and smaller polypeptides, and the other was a homodimer of a 46-kDa polypeptide. Three different purified rat peroxisomal D-3-hydroxyacyl-CoA dehydratase preparations have been ...

journal_title：Journal of biochemistry

authors： Jiang LL,Miyazawa S,Hashimoto T

更新日期：1996-09-01 00:00:00

abstract：:Phosphorylation of chicken gizzard myosin light chain in myofibril and its effect on myofibrillar ATPase activity were investigated in the contracted state of myofibrils. When myofibrils were incubated for two hours at 30 degreeds C with ATP, magnesium and calcium, the myosin light chain was phosphorylated by endogeno...

journal_title：Journal of biochemistry

authors： Yamaguchi M,Watanabe K

更新日期：1980-04-01 00:00:00

abstract：:We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

journal_title：Journal of biochemistry

authors： Yamamoto K

更新日期：1993-12-01 00:00:00

abstract：:A crude preparation of membrane-bound phospholipase A (detergent-resistant) in Escherichia coli K-12 cells was found to be quite stable or even apparently activated on incubation at 100 degrees C, but became strikingly thermolabile when it was highly purified and Triton X-100 was removed from the purified enzyme prepa...

journal_title：Journal of biochemistry

authors： Tamori Y,Nishijima M,Nojima S

更新日期：1979-10-01 00:00:00

abstract：:Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC 4.1.2.13] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

journal_title：Journal of biochemistry

authors： Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

更新日期：1991-04-01 00:00:00

abstract：:A beta-galactosidase was extracted from the internal organs of a sea squirt, Styela plicata, and purified 959-fold, with an 18% yield, by successive gel chromatography, anion-exchange chromatography, chromatofocusing, and affinity chromatography on a Con A-Sepharose column. The purified enzyme was fairly homogeneous, ...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Oka S

更新日期：1991-07-01 00:00:00

abstract：:We have purified L-kynurenine 3-monooxygenase from pig liver mitochondria using a procedure involving seven steps composed of (1) preparation of mitochondrial outer membrane, (2) preparation of the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) insoluble outer membrane mater...

journal_title：Journal of biochemistry

authors： Uemura T,Hirai K

更新日期：1998-02-01 00:00:00

abstract：:A GTP-binding protein with an Mr of 24,000 was purified from a cholate extract of bovine brain membranes in addition to the previously reported alpha beta gamma-trimeric GTP-binding proteins (G proteins). Partial amino acid sequence analysis of the purified 24-kDa protein revealed that it was not identical to any of t...

journal_title：Journal of biochemistry

authors： Ohoka Y,Imai S,Kozasa T,Maehama T,Takahashi K,Kaziro Y,Ui M,Katada T

更新日期：1991-03-01 00:00:00

abstract：:A novel glycosphingolipid (SEGLx) has been isolated from the plerocercoids of a tapeworm, Spirometra erinacei. From the results of compositional analysis, methylation analysis, exoglycosidase hydrolysis, acid hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance (NMR) analysis, its...

journal_title：Journal of biochemistry

authors： Kawakami Y,Nakamura K,Kojima H,Suzuki M,Inagaki F,Suzuki A,Sonoki S,Uchida A,Murata Y,Tamai Y

更新日期：1993-11-01 00:00:00

abstract：:Vertebrate skeletal muscle alpha-tropomyosin polymerizes in a head-to-tail manner and binds cooperatively to actin. It has been postulated that the cooperative actin binding is governed by the strength of the head-to-tail interaction. In order to know the relationship between the head-to-tail affinity and actin bindin...

journal_title：Journal of biochemistry

authors： Sano K,Maeda K,Oda T,Maéda Y

更新日期：2000-06-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:Under conditions where nM level of calmodulin was able to show full activation of myosin light chain kinase and cyclic-nucleotide phosphodiesterase, the fragments of calmodulin at concentrations as high as 20 microM failed to activate these enzymes in the presence of Ca2+. The fragments tested were Ala1-Lys75 (F12), A...

journal_title：Journal of biochemistry

authors： Minowa O,Yazawa M,Sobue K,Ito K,Yagi K

更新日期：1988-03-01 00:00:00

abstract：:Several trypsin inhibitors with different mobilities on polyacrylamide gel electrophoresis occur in the tubers of taro (Colocasia antiquorum), and they each have a dimeric molecular weight of 40,000. Of all the constituent subunits, molecular weight 20,000, of the taro trypsin inhibitor (TTI), three major subunit comp...

journal_title：Journal of biochemistry

authors： Ogata F,Makisumi S

更新日期：1985-02-01 00:00:00

abstract：:By analyzing antibody heterogeneity during the primary immune response to bacterial a-amylase (B alpha A) in high-responder F1 hybrid mice between C57BL/6 (B6) and C3H/He (C3) mice with the use of isoelectric focusing (IEF), it was shown that the maturation of the primary IgG antibody response consisted of at least tw...

journal_title：Journal of biochemistry

authors： Nakashima S,Kamikawa H

更新日期：1984-07-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00