Purification and characterization of a new GTP-binding protein of Mr 24,000 in bovine brain membranes.

abstract：:Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

journal_title：Journal of biochemistry

authors： Mima J,Suzuki H,Takahashi M,Hayashi R

更新日期：2002-12-01 00:00:00

abstract：:Human serum sulfotransferase activities were measured in normal controls and patients with macular corneal dystrophy (MCD), an inherited disorder characterized by the decreased sulfation of keratan sulfate in the corneal stroma and serum, using two kinds of acceptor: partially desulfated keratan sulfate and a trisacch...

journal_title：Journal of biochemistry

authors： Hasegawa N,Torii T,Nagaoka I,Nakayasu K,Miyajima H,Habuchi O

更新日期：1999-02-01 00:00:00

abstract：:The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification. The purified preparation of the reductase showed an apparent molecular weight of 27,000 daltons. The reductase appeared to contain loosely-bound FAD as a prosthetic gr...

journal_title：Journal of biochemistry

authors： Kubota S,Yoshida Y,Kumaoka H

更新日期：1977-01-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:The sensitivity and specificity of two influenza C virus assays, solid-phase and overlay assays, were investigated using naturally occurring 9-O-acetylated GD(3), rat serum glycoproteins containing 60% of N-acetyl-9-O-acetylneuraminic acid, and synthetically O-acetylated sialylated compounds. The sensitivity of the so...

journal_title：Journal of biochemistry

authors： Hubl U,Ishida H,Kiso M,Hasegawa A,Schauer R

更新日期：2000-06-01 00:00:00

abstract：:In the preceding paper [Maita, T., Miyanishi, T., Matsuzono, K., Tanioka, Y., & Matsuda, G. (1991) J. Biochem. 110, 68-74], we reported the amino-terminal 837-residue sequence of the heavy chain of adult chicken pectoralis muscle myosin. This paper describes the carboxyl terminal 1,097-residue sequence and the linkage...

journal_title：Journal of biochemistry

authors： Maita T,Yajima E,Nagata S,Miyanishi T,Nakayama S,Matsuda G

更新日期：1991-07-01 00:00:00

abstract：:The structure and expression of the pentraxins, serum amyloid P component (SAP), and C-reactive protein (CRP), have been investigated in the guinea pig. Northern blot analysis of hepatic RNA from animals in which acute inflammation had been induced by intraperitoneal injection of thioglycollate established that neithe...

journal_title：Journal of biochemistry

authors： Rubio N,Sharp PM,Rits M,Zahedi K,Whitehead AS

更新日期：1993-03-01 00:00:00

abstract：:The state of association of bovine Band 3, which is a major intrinsic protein of erythrocyte membranes, was examined in nonaethyleneglycol dodecyl ether (C12E9) solution by ultracentrifugation, gel filtration, gel electrophoresis and cross-linking studies. The molecular weight of bovine Band 3 was 107,000 +/- 5% as de...

journal_title：Journal of biochemistry

authors： Nakashima H,Makino S

更新日期：1980-10-01 00:00:00

abstract：:We have studied the regio- and stereoselectivity of ring-hydroxylation and N-desisopropylation of S(-)- and R(+)-propranolol, using dog liver microsomes and the expressed dog CYP2D15 in insect cells. In dog liver microsomes, 4-hydroxylation was the preferred pathway in S(-)-propranolol oxidation, while N-desisopropyla...

journal_title：Journal of biochemistry

authors： Tasaki T,Iwata H,Kazusaka A,Fujita S

更新日期：1998-04-01 00:00:00

abstract：:Activity-oligomeric assembly relationships using octaethylene glycol dodecyl ether (C12E8) solubilized pig gastric H/K-ATPase (unmodified H/K-ATPase) or H/K-ATPase modified with Fluorescein 5'-isothiocyanate (FITC-H/K-ATPase) were examined. The amount of oligomeric species in FITC-H/K-ATPase, which retained little H/K...

journal_title：Journal of biochemistry

authors： Abe K,Kaya S,Taniguchi K,Hayashi Y,Imagawa T,Kikumoto M,Oiwa K,Sakaguchi K

更新日期：2005-09-01 00:00:00

abstract：:Ectoenzyme release from rat liver and kidney by phosphatidylinositol (PI)-specific phospholipase C of Bacillus thuringiensis was studied. Alkaline phosphatase and 5'-nucleotidase were released from rat kidney slices to extents of up to 60% and 30%, respectively. Release of alkaline phosphatase was observed at lower am...

journal_title：Journal of biochemistry

authors： Taguchi R,Asahi Y,Ikezawa H

更新日期：1985-03-01 00:00:00

abstract：:An improved procedure for the purification of a lipid-containing acidophilic thermophilic bacteriophage, phiNS11, is described. The procedure involves Cs2SO4 discontinuous density gradient, Cs2SO4 equilibrium density gradient and sucrose gradient centrifugations. The phage has an icosahedral shape, 75 nm in diameter, ...

journal_title：Journal of biochemistry

authors： Sakaki Y,Yamada K,Oshima M,Oshima T

更新日期：1977-11-01 00:00:00

abstract：:The pH-activity curve of heavy meromyosin ATPase [EC 3.6.1.3] was measured at various temperatures. The pH-activity curve at higher temperatures showed a maximum at low pH and a minimum at pH 7 to 8 as has been already reported. At lower temperatures it was sigmoidal in shape, similar to a simple dissociation curve of...

journal_title：Journal of biochemistry

authors： Ishigami F,Morita F

更新日期：1977-02-01 00:00:00

abstract：:The role of thiols on the activation and/or stabilization of rat brain nitric oxide synthase (NOS) has been investigated. It was found that thiols are not necessary for stabilizing or protecting the protein during purification but are required during enzyme turnover for maximum activity. In the complete absence of thi...

journal_title：Journal of biochemistry

authors： Komori Y,Hyun J,Chiang K,Fukuto JM

更新日期：1995-04-01 00:00:00

abstract：:The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein of 286 amino acids. The amino acid sequence shared approximately 80-90% similarity to the Bacillus ...

journal_title：Journal of biochemistry

authors： Ertunga NS,Colak A,Belduz AO,Canakci S,Karaoglu H,Sandalli C

更新日期：2007-06-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00

abstract：:Gram-positive thermophilic Bacillus species contain cytochrome caa3-type cytochrome c oxidase as their main terminal oxidase in the respiratory chain. To identify alternative oxidases, we isolated several mutants from B. stearothermophilus defective in the caa3-type oxidase activity [Sakamoto, J. et al (1996) FEMS Mic...

journal_title：Journal of biochemistry

authors： Sakamoto J,Handa Y,Sone N

更新日期：1997-10-01 00:00:00

abstract：:Escherichia coli RNase HII is composed of 198 amino acid residues. The enzyme has been overproduced in an insoluble form, purified in a urea-denatured form, and refolded with poor yield [M. Itaya (1990) Proc. Natl. Acad. Sci. USA 87, 8587-8591]. To facilitate the preparation of the enzyme in an amount sufficient for p...

journal_title：Journal of biochemistry

authors： Ohtani N,Haruki M,Muroya A,Morikawa M,Kanaya S

更新日期：2000-05-01 00:00:00

abstract：:The effects of various compounds on ascorbate-2-sulfate sulfohydrolase and arylsulfatase (EC 3.1.6.1) activities in the copurified preparation from the liver of Charonia lampas were investigated. The former activity was competively inhibited by inorganic phosphate and sulfate. The latter was not affected by sulfate....

journal_title：Journal of biochemistry

authors： Hatanaka H,Ogawa Y,Egami F

更新日期：1975-04-01 00:00:00

abstract：:The DNA-dependent protein kinase (DNA-PK) holoenzyme consists of a 470-kDa catalytic subunit (DNA-PKcs), a DNA-binding regulatory component known as Ku protein, and double-stranded DNA (dsDNA) with ends. We previously reported that the activity of DNA-PK in vitro is stimulated by non-histone chromosomal high mobility ...

journal_title：Journal of biochemistry

authors： Yumoto Y,Shirakawa H,Yoshida M,Suwa A,Watanabe F,Teraoka H

更新日期：1998-09-01 00:00:00

abstract：:Three alkaline DNases, A, B, and C, with preference for the digestion of double-stranded DNA (dsDNA) were partially purified from microplasmodia of Physarum polycephalum. They were very similar but differed in their isoelectric points. These were pH 5.8 for DNase A, 7.1 for DNase B, and 9.1 for DNase C. All three enzy...

journal_title：Journal of biochemistry

authors： Waterborg JH,Kuyper CM

更新日期：1980-02-01 00:00:00

abstract：:Two forms of urokinase [EC 3.4.99.26] with molecular weights of 51,600 and 34,500 were purified from human urine. The specific activities of the high molecular weight urokinase (HMW-UK) and low molecular weight urokinase (LMW-UK) were 157,400 and 246,700 International Units (IU/mg), respectively. Purified HMW-UK was 9...

journal_title：Journal of biochemistry

authors： Nobuhara M,Sakamaki M,Ohnishi H,Suzuki Y

更新日期：1981-07-01 00:00:00

abstract：:The complete amino acid sequence and disulfide bridge location of HR2a, one of the hemorrhagic proteins isolated from the snake venom of Trimeresurus flavoviridis, have been determined by analysis of peptides derived from digests with cyanogen bromide, lysyl endopeptidase, trypsin, and Staphylococcus aureus V8 proteas...

journal_title：Journal of biochemistry

authors： Miyata T,Takeya H,Ozeki Y,Arakawa M,Tokunaga F,Iwanaga S,Omori-Satoh T

更新日期：1989-05-01 00:00:00

abstract：:Changes in the fatty acid composition of choline glycerophospholipid (CGP) and ethanolamine glycerophospholipid (EGP) in the liver, heart, kidney, testis, spleen, and brain of rats were examined at various stages of streptozotocin-induced diabetes. Diabetes of prolonged duration (8 weeks) caused profound alterations i...

journal_title：Journal of biochemistry

authors： Hu Q,Ishii E,Nakagawa Y

更新日期：1994-03-01 00:00:00

abstract：:A new multifunctional protein kinase, which normally exists as an inactive form in the soluble fraction in mammalian tissues, attaches to membranes to exhibit full enzymatic activity. A low concentration of Ca2+ is absolutely necessary for this activation. This process is reversible. cAMP shows no effect. The active f...

journal_title：Journal of biochemistry

authors： Takai Y,Kishimoto A,Iwasa Y,Kawahara Y,Mori T,Nishizuka Y,Tamura A,Fujii T

更新日期：1979-08-01 00:00:00

abstract：:ENO1-'lacZ fusions with various lengths of the ENO1 5'-flanking region were constructed on various types of yeast plasmid vectors. The fully expressed level of beta Gal directed by ENO1-'lacZ fusions differed depending on the type of vector, but on any type of vector, beta Gal activity was not greatly influenced by th...

journal_title：Journal of biochemistry

authors： Uemura H,Shiba T,Machida M,Matsui I,Jigami Y,Tanaka H

更新日期：1987-07-01 00:00:00

abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...

journal_title：Journal of biochemistry

authors： Waki H,Murata A,Kon K,Maruyama K,Kimura S,Ogura H,Ando S

更新日期：1993-04-01 00:00:00

abstract：:The sequence of the 5'-terminal 106 nucleotides of cucumber mosaic virus (strain Y) RNA 4, the mRNA coding for viral coat protein, has been determined. The first AUG was located at 77 nucleotides from the 5'-terminus and was confirmed to be an initiation codon by analysis of the N-terminal amino acid sequence of the p...

journal_title：Journal of biochemistry

authors： Hidaka S,Tsunasawa S,Yoon J,Narita K,Takanami Y,Kubo S,Miura K

更新日期：1985-01-01 00:00:00

abstract：:Temperature and solvent effects on reaction center structures were examined in two thermophilic photosynthetic bacteria, Chloroflexus aurantiacus and Chromatium tepidum, in order to gain insight into the interactions among the reaction center proteins and pigment systems. Thermal stability of the reaction centers was ...

journal_title：Journal of biochemistry

authors： Nozawa T,Madigan MT

更新日期：1991-10-01 00:00:00

abstract：:p40(phox) of the phagocyte NADPH oxidase forms a complex with p67(phox) in cytosol, and coincidentally decreases in patients who lack p67(phox). Here we investigated the mode of translocation of p40(phox) to the membrane, its cytoskeletal localization on activation of the NADPH oxidase, and the dependency of its expre...

journal_title：Journal of biochemistry

authors： Tsunawaki S,Yoshikawa K

更新日期：2000-11-01 00:00:00