Unexpectedly general replaceability of ATP in ATP-requiring enzymes.

abstract：:The incorporation of serine and ethanolamine into phospholipids in rabbit retinal subcellular fractions and in excised retinas was studied in vitro, and some enzymic properties of the incorporation of phospholipid bases by base exchange were examined in the microsomal fraction. The retina was found to have a higher ra...

journal_title：Journal of biochemistry

authors： Mizuno A

更新日期：1976-07-01 00:00:00

abstract：:We synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. We solubilized membrane proteins of Vibrio parahaemolyticus using the detergents. With octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfacto...

journal_title：Journal of biochemistry

authors： Izawa S,Sakai-Tomita Y,Kinomura K,Kitazawa S,Tsuda M,Tsuchiya T

更新日期：1993-05-01 00:00:00

abstract：:Mammalian/mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase that responds to environmental determinants such as growth factor concentration, nutrient availability, energy sufficiency and stress. mTOR forms two different complexes, called mTOR complex 1 (mTORC1) and mTORC2, and these ...

journal_title：Journal of biochemistry

authors： Hoshii T,Matsuda S,Hirao A

更新日期：2014-08-01 00:00:00

abstract：:Several kinds of hydrophilic proteins were examined to determine their interaction with artificial liposomes. Mitochondrial aspartate aminotransferase (m-GOT) [EC 2.6.1.1], as well as cytochrome c, was found to interact strongly with negatively charged liposomes. In each case, an appreciable amount of the protein boun...

journal_title：Journal of biochemistry

authors： Furuya E,Yoshida Y,Tagawa K

更新日期：1979-05-01 00:00:00

abstract：:A membrane protein insoluble in water was isolated by gel chromatography in the presence of 0.1% sodium dodecyl sulfate (SDS) from chromatophores of a photosynthetic bacterium, Rhodospirillum rubrum. This is one of the major membrane proteins of the chromatophore. The protein was found to bind about four grams of SDS ...

journal_title：Journal of biochemistry

authors： Miyake J,Ochiai-Yanagi S,Kasumi T,Takagi T

更新日期：1978-06-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Katsuda N,Kato K

更新日期：1980-02-01 00:00:00

abstract：:We have reported that high glucose conditions (27 mM for 4 days) induces activation of protein tyrosine phosphatases (PTPases) which are associated with impaired insulin signaling in Rat 1 fibroblasts expressing human insulin receptors [Maegawa, H. et al. (1995) J. Biol. Chem. 270, 7724-7730]. In this study, we found ...

journal_title：Journal of biochemistry

authors： Obata T,Maegawa H,Kashiwagi A,Pillay TS,Kikkawa R

更新日期：1998-05-01 00:00:00

abstract：:Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

journal_title：Journal of biochemistry

authors： Shishido H,Yamada MD,Kondo K,Maruta S

更新日期：2009-10-01 00:00:00

abstract：:When the body temperature of rats was brought to 42 degrees C, four heat-shock proteins, with molecular weights of 70,000, 71,000, 85,000, and 100,000 (hsp 70, hsp 71, hsp 85, and hsp 100, respectively), were induced in various tissues of the rats. The hsp 70 was strongly induced by hyperthermia, and its accumulation ...

journal_title：Journal of biochemistry

authors： Fujio N,Hatayama T,Kinoshita H,Yukioka M

更新日期：1987-01-01 00:00:00

abstract：:Gastric cancer (GC) is one of the most common cancers in gastrointestinal malignant tumours. Long non-coding RNAs were widely reported to play a significant role in the regulation of occurrence or development of tumours. Bioinformatics analysis and a wide range of experiments were conducted to explore the expression s...

journal_title：Journal of biochemistry

authors： Xin H,Yan Z,Cao J

更新日期：2020-11-01 00:00:00

abstract：:Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC 4.1.2.13] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

journal_title：Journal of biochemistry

authors： Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

更新日期：1991-04-01 00:00:00

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:Inhibitory effects of various amino acid esters on the phagocytic activity of guinea pig peritoneal macrophages were studied with sensitized 51Cr-sheep erythrocytes (51Cr-EAb) as well as 125I-alpha-amylase complexed with homologous IgG2 antibody (Ag-Ab complex). The intracellular uptake of 51Cr-EAb was markedly inhibi...

journal_title：Journal of biochemistry

authors： Nihira S,Koyama J

更新日期：1983-08-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00

abstract：:Synthesis and degradation of glycerophospholipids in HL-60 cells and retinoic acid (RA)-treated HL-60 cells were examined. The synthesis of each subclass of ethanolamine-containing glycerophospholipids was extremely suppressed in RA-treated HL-60 cells, while that of other glycerophospholipids was not seriously affect...

journal_title：Journal of biochemistry

authors： Naito M,Kudo I,Nakagawa Y,Waku K,Nojiri H,Saito M,Inoue K

更新日期：1991-10-01 00:00:00

abstract：:2,3-Bisphosphoglycerate mutase (BPGM) [EC 5.4.2.4] is a multifunctional enzyme that catalyzes both the synthesis and the degradation of 2,3-diphosphoglycerate (2,3-DPG) and contains three types of activities in that it functions as a 2,3-DPG synthetase, a phosphoglycerate mutase and a 2,3-DPG phosphatase. In humans, B...

journal_title：Journal of biochemistry

authors： Fujita T,Suzuki K,Tada T,Yoshihara Y,Hamaoka R,Uchida K,Matuo Y,Sasaki T,Hanafusa T,Taniguchi N

更新日期：1998-12-01 00:00:00

abstract：:By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

journal_title：Journal of biochemistry

authors： Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

更新日期：1982-06-01 00:00:00

abstract：:The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification. The purified preparation of the reductase showed an apparent molecular weight of 27,000 daltons. The reductase appeared to contain loosely-bound FAD as a prosthetic gr...

journal_title：Journal of biochemistry

authors： Kubota S,Yoshida Y,Kumaoka H

更新日期：1977-01-01 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain ...

journal_title：Journal of biochemistry

authors： Amaya Y,Nakai T,Miura S

更新日期：2016-04-01 00:00:00

abstract：:The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

journal_title：Journal of biochemistry

authors： Giga Y,Ikai A

更新日期：1985-07-01 00:00:00

abstract：:Chemical modification of gamma-carboxyglutamic acid (Gla) residues in human prothrombin to gamma-methyleneglutamic acid (gamma-MGlu) residues elicited a conformation similar, if not identical, to that of des-gamma-carboxy prothrombin or PIVKA-II, i.e., prothrombin molecules induced by vitamin K antagonists or vitamin ...

journal_title：Journal of biochemistry

authors： Sugo T,Watanabe K,Naraki T,Matsuda M

更新日期：1990-09-01 00:00:00

abstract：:We characterized the taurine transport system in human intestinal Caco-2 cells and showed that it is subject to adaptive regulation. The activity of taurine transport in Caco-2 cells was evaluated by means of an Na+- and Cl(-)-dependent high-affinity transport system, the characteristics of which were similar to those...

journal_title：Journal of biochemistry

authors： Satsu H,Watanabe H,Arai S,Shimizu M

更新日期：1997-06-01 00:00:00

abstract：:The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

journal_title：Journal of biochemistry

authors： Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:Irradiation of isolated chloroplast thylakoids with TNP-ADP results in non-covalent binding and covalent incorporation of a reaction product of TNP-ADP formed by photosynthetic reduction into the so-called "tight" nucleotide binding site of CF1 [Ponse et al. (1992) Z. Naturforsch. 47c, 264-274]. CF1 extracted from thu...

journal_title：Journal of biochemistry

authors： Hisabori T,Kothen G,Strotmann H

更新日期：1993-09-01 00:00:00

abstract：:The scaffolding protein Salvador (Sav) plays a key role in the Hippo (Hpo) signalling pathway, which controls tissue growth by inhibiting cell proliferation and promoting apoptosis. Dysregulation of the Hippo pathway contributes to cancer development. Since the identification of the first Sav gene in 2002, very little...

journal_title：Journal of biochemistry

authors： Song J,Hong HR,Yamashita E,Park IY,Lee SJ

更新日期：2015-09-01 00:00:00

abstract：:Guanosine 3',5'-monophosphate (cyclic GMP)-dependent protein kinase purified from silkworm pupae reacts with rat liver ribosomal proteins when a stimulatory modulator (Kuo, W.N. & Kuo, J.F. 1976) J. Biol. Chem. 251, 4283-4286) is added to the reaction mixture. Judging from autoradiogram of the radioactive proteins sep...

journal_title：Journal of biochemistry

authors： Chihara-Nakashima M,Hashimoto E,Nishizuka Y

更新日期：1977-06-01 00:00:00

abstract：:Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-ac...

journal_title：Journal of biochemistry

authors： Kobayashi K,Morisawa Y,Ishituka T,Ishimoto M

更新日期：1975-11-01 00:00:00

abstract：:Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-compl...

journal_title：Journal of biochemistry

authors： Baba S,Takahashi K,Noguchi S,Takaku H,Koyanagi Y,Yamamoto N,Kawai G

更新日期：2005-11-01 00:00:00