Establishment and characterization of a novel human rheumatoid fibroblast-like synoviocyte line, MH7A, immortalized with SV40 T antigen.

abstract：:In vitro activation of human reovirus transcriptase by alpha-chymotrypsin digestion of viral outer shell proteins was completely dependent on the ionic size of the monovalent cation in the medium. Cations with nonhydrated ionic radii larger than 1.3 A showed full potency of activation of chymotrypsin digestion, and pr...

journal_title：Journal of biochemistry

authors： Nagai J,Tanaka M,Nakashima K

更新日期：1984-03-01 00:00:00

abstract：:2-Amino-4-methylphenol was converted to a brownish yellow material by the lysates of human erythrocytes or purified human hemoglobin. The reaction proceeded oxidatively, coupled with the oxidation of hemoglobin. The major component of the brownish yellow material produced by oxidative condensation of 2-amino-4-methylp...

journal_title：Journal of biochemistry

authors： Tomoda A,Arisawa M,Koshimura S

更新日期：1991-12-01 00:00:00

abstract：:The whole-genome sequencing of an extreme thermophile, Thermus thermophilus, is now in progress. Like other genome projects, major concern is shifting from the sequence itself to post-sequencing research such as functional or structural genomics. Under such circumstances, the demand for convenient genetic-engineering ...

journal_title：Journal of biochemistry

authors： Hoseki J,Yano T,Koyama Y,Kuramitsu S,Kagamiyama H

更新日期：1999-11-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...

journal_title：Journal of biochemistry

authors： Okazaki N,Hazeki K,Izumi T,Nigorikawa K,Hazeki O

更新日期：2011-03-01 00:00:00

abstract：:Calf thymus DNA polymerases alpha and beta [EC 2.7.7.7] and terminal deoxynucleotidyl transferase [EC 2.7.7.31] were analyzed on two-dimensional gel slabs. DNA polymerase beta appeared as a single spot on two-dimensional gel at the position of 40,000 daltons and pI 8.0 using non-equilibrium pH gradient gel electrophor...

journal_title：Journal of biochemistry

authors： Koiwai O,Masaki S,Kaneda T,Yoshida S

更新日期：1982-08-01 00:00:00

abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...

journal_title：Journal of biochemistry

authors： Poetsch A,Berzborn RJ,Heberle J,Link TA,Dencher NA,Seelert H

更新日期：2007-03-01 00:00:00

abstract：:The present investigation was undertaken to see to what extent the alpha-amino group of the amino acid, the side chain of the amino acid of aminoacyl-tRNA, and the tRNA structure are involved in determining the affinity of aminoacyl-tRNA for bacterial elongation factor Tu-GTP complex. Various aminoacyl-tRNAs, mis-amin...

journal_title：Journal of biochemistry

authors： Tanada S,Kawakami M,Nishio K,Takemura S

更新日期：1982-01-01 00:00:00

abstract：:We have detected in rat liver cytosol three enzymes (termed C-1, C-2, and C-3) which cleaved the RNA moiety of RNA-DNA hybrid. These enzymes were separated from each other by DEAE-Sephadex and Sephadex G-200 chromatography. C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, while C-3 degrades single-str...

journal_title：Journal of biochemistry

authors： Tashiro F,Ueno Y

更新日期：1978-08-01 00:00:00

abstract：:A protease activity specific to spermatogenous histones was found in the egg extract of sea urchin. The enzyme responsible for this activity, named SPKK protease because of its substrate specificity, was purified as a monomeric 28 kDa protein. SPKK protease activity is inhibited by leupeptin and is specific to the rep...

journal_title：Journal of biochemistry

authors： Suzuki M,Sugiura M,Ebashi S

更新日期：1990-09-01 00:00:00

abstract：:Calphobindins (CPBs, placental annexins) are intracellular Ca(2+)- and phospholipid-dependent proteins like protein kinase C [EC 2.7.1.37]. We investigated the inhibitory effects of calphobindins on the protein kinase C activity in vitro. CPB I inhibited the protein kinase C activity for both histone phosphorylation a...

journal_title：Journal of biochemistry

authors： Shibata S,Sato H,Maki M

更新日期：1992-10-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:Neutral alpha-glucosidase was partially purified from granular fractions isolated from guinea pig polymorphonuclear leukocytes (PMNL). The native enzyme had a high molecular weight, about 417,000, with a subunit of 43,000. The purified enzyme hydrolysed 4-methylumbelliferyl alpha-glucoside and maltose, but not isomalt...

journal_title：Journal of biochemistry

authors： Imai K,Harada T,Takano Y,Morikawa S,Tanaka A

更新日期：1989-10-01 00:00:00

abstract：:A new p-coumaric acid (4-hydroxycinnamic acid) hydroxylase was detected in mung bean seedlings treated with tentoxin, a fungal toxin, in which polyphenol oxidase that hydroxylates a wide variety of monophenols in vitro was completely eliminated. The enzyme required molecular oxygen and showed a pH optimum of 5.0. The ...

journal_title：Journal of biochemistry

authors： Kojima M,Takeuchi W

更新日期：1989-02-01 00:00:00

abstract：:The pcd gene from Flavobacterium lutescens IFO3084 encoding Delta'-piperideine-6-carboxylate dehydrogenase (PCD) was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence of PCD from F. lutescens IFO3084 showed strong similarity to that from Streptomyces clavuligerus. The molecular mass...

journal_title：Journal of biochemistry

authors： Fujii T,Narita T,Agematu H,Agata N,Isshiki K

更新日期：2000-12-01 00:00:00

abstract：:Lactosylceramide and paragloboside were synthesized from their precursor glycolipids and UDP-galactose by lactose synthase A protein [UDP-Gal : GlcNAc beta-4-galactosyltransferase, EC 2.4.1.22] purified to homogeneity from human plasma. The partially purified human liver enzyme and an extract from human lymphoblastoid...

journal_title：Journal of biochemistry

authors： Yamato K,Yoshida A

更新日期：1982-10-01 00:00:00

abstract：:Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

journal_title：Journal of biochemistry

authors： Narutaki S,Dunn BM,Oda K

更新日期：1999-01-01 00:00:00

abstract：:The activity of thermolysin is greatly enhanced in the presence of high concentrations of neutral salts [Holmquist, B. and Vallee, B.L. (1976) Biochemistry 15, 101-107; Inouye, K. (1992) J. Biochem. 112, 335-340]. NaBr and NaCl are the most effective for the activation. An absorption difference spectrum with a peak ar...

journal_title：Journal of biochemistry

authors： Inouye K,Kuzuya K,Tonomura B

更新日期：1994-09-01 00:00:00

abstract：:We studied the mechanism of formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study. The hydroxyl radical is also generated through Fenton t...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Tero-Kubota S,Yamamoto Y,Kato M,Nakano M,Yagi K,Ogino K

更新日期：2003-12-01 00:00:00

abstract：:It is not known whether the activity of the Escherichia coli protein export system changes during the division cycle. To address and answer this question, we took two approaches. First, we pulse-labeled a random culture and size-fractionated the labeled cells in the presence of inhibitors of secretion. Second, we puls...

journal_title：Journal of biochemistry

authors： Taura T,Ito K

更新日期：1991-06-01 00:00:00

abstract：:1. From the standpoint of monomer-dimer equilibrium of hog kidney D-amino acid oxidase [EC 1.4.3.3] and the interaction between the enzyme and small molecules, the effect of pH on the binding of p-aminobenzoate to the monomer and dimer of the enzyme was studied by kinetic methods and spectrophotometric titration. 2. T...

journal_title：Journal of biochemistry

authors： Horiike K,Shiga K,Isomoto A,Yamano T

更新日期：1976-11-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) membranes of rabbit skeletal muscle were allowed to react with N-(3-pyrene)maleimide (PMI) at pH 7 at 30 degrees C. The Ca(2+)-transporting activity of the SR membranes was reduced to 20% when PMI was bound to the extent of 1 mol/mol of Ca(2+)-transporting ATPase. The ATPase and the E-P...

journal_title：Journal of biochemistry

authors： Suzuki T,Kawakita M

更新日期：1993-08-01 00:00:00

abstract：:Saccharomyces cerevisiae has three distinct citrate synthases, two located in mitochondria (mature Cit1p and Cit3p) and one in peroxisomes (mature Cit2p). While the precursor of the major mitochondrial enzyme, Cit1p, has a signal for mitochondrial targeting at its N-terminus (MTS), Cit2p has one for peroxisomal target...

journal_title：Journal of biochemistry

authors： Lee JG,Cho SP,Lee HS,Lee CH,Bae KS,Maeng PJ

更新日期：2000-12-01 00:00:00

abstract：:A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

journal_title：Journal of biochemistry

authors： Sone N,Kutoh E,Sato K

更新日期：1990-04-01 00:00:00

abstract：:The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

journal_title：Journal of biochemistry

authors： Hoshita T,Harada N,Morita I,Kihira K

更新日期：1981-11-01 00:00:00

abstract：:Diacylglycerol acyltransferase (DGAT) was examined as a key enzyme for triacylglycerol (TG) accumulation of an oleaginous fungus, Mortierella ramanniana var. angulispora. Subcellular fractionation of the fungus showed that DGAT activity was highest in the lipid body fraction, which occupied 77% of the recovered DGAT a...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Nakahara T

更新日期：1994-12-01 00:00:00

abstract：:The bacterial opsins can be refolded to regenerate the chromophore by transfer from SDS to DMPC/CHAPS/SDS mixed micelles in the presence of retinal. A sequential refolding model has been proposed for bacterioopsin [Booth et al. (1995) Nature Struct. Biol. 2, 139-143]. However, the roles of DMPC and CHAPS in the refold...

journal_title：Journal of biochemistry

authors： Sugiyama Y,Mukohata Y

更新日期：1996-06-01 00:00:00

abstract：:The effects of Na+ and ATP on the K+ binding to Na+, K+-ATPase were investigated by the centrifugation method with radioactive K+ in the absence of Mg2+. In the presence of 10 microM 43KCl, 0.6 and 10 mM Na+ decreased the amount of bound K+ to one-half and zero, respectively. On the other hand, 10 microM and 10 mM ATP...

journal_title：Journal of biochemistry

authors： Homareda H,Nozaki T,Matsui H

更新日期：1987-03-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:Protein carbonyls are reported to increase in aging and in pathologies such as Alzheimer's disease and ischemic injury. Detailed study of this important issue has, however, been hampered by lack of an appropriate method to identify individual carbonylated proteins. We describe here an immunoblot method to identify ind...

journal_title：Journal of biochemistry

authors： Nakamura A,Goto S

更新日期：1996-04-01 00:00:00