Abstract:
:Calphobindins (CPBs, placental annexins) are intracellular Ca(2+)- and phospholipid-dependent proteins like protein kinase C [EC 2.7.1.37]. We investigated the inhibitory effects of calphobindins on the protein kinase C activity in vitro. CPB I inhibited the protein kinase C activity for both histone phosphorylation and lipocortin phosphorylation, but CPB II and CPB III inhibited only the protein kinase C activity for histone phosphorylation. In the case of histone phosphorylation, all CPBs inhibited the protein kinase C activity in a concentration-dependent manner, and the IC50 (concentration required for 50% inhibition) value of CPB I was 70 nM. The inhibition of protein kinase C by CPB I was Ca(2+)-dependent, and did not disappear upon increasing the concentration of phosphatidyl-serine. Kinetic analysis by double-reciprocal plots indicated that CPB I interacted not only with phosphatidylserine but also with protein kinase C. Although CPB I partially interacts with phospholipid, it is conceivable that the inhibitory action of CPB I on protein kinase C results from direct interaction of CPB I with protein kinase C. Since CPBs are mainly present under the plasma membrane, it is presumed that CPB I is an endogenous inhibitor of protein kinase C, and according to intracellular circumstances, CPB II and CPB III may also be endogenous inhibitors.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Shibata S,Sato H,Maki Mdoi
10.1093/oxfordjournals.jbchem.a123937keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
552-6issue
4eissn
0021-924Xissn
1756-2651journal_volume
112pub_type
杂志文章abstract::The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis tra...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1992-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-08-01 00:00:00
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更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124636
更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00
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更新日期:2013-08-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1984-06-01 00:00:00
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pub_type: 杂志文章
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