10Sa RNA is associated with 70S ribosome particles in Escherichia coli.

abstract：:During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

journal_title：Journal of biochemistry

authors： Iwamuro S,Saeki M,Kato S

更新日期：1999-07-01 00:00:00

abstract：:We examined the substrate specificity of two mutated geranylgeranyl diphosphate synthases, I-9 and I-11, with respect to several artificial substrates. These mutated enzymes have replacements in the amino acid sequences from positions 170 to 173, which are thought to be a part of the putative substrate binding region....

journal_title：Journal of biochemistry

authors： Ohnuma S,Hemmi H,Koyama T,Ogura K,Nishino T

更新日期：1998-06-01 00:00:00

abstract：:The aim of this work was to investigate the putative modulation of glucose uptake in trophoblast cells by several dietary compounds and by drugs of abuse. For this, the acute (26 min) and chronic (48 h) effect of these substances on the apical uptake of 3H-2-deoxy-D-glucose (3H-DG) by a human choriocarcinoma cell line...

journal_title：Journal of biochemistry

authors： Araújo JR,Gonçalves P,Martel F

更新日期：2008-08-01 00:00:00

abstract：:Tetrahydrobiopterin (BH(4)) acts as a cofactor of the aromatic amino-acid hydroxylases, and its deficiency may result in hyperphenylalaninemia (HPA) and decreased production of the neurotransmitters. BH(4) is synthesized by sepiapterin reductase (SPR) from 6-pyruvoyl-tetrahydropterin (PPH(4)). A patient with SPR defic...

journal_title：Journal of biochemistry

authors： Hirakawa H,Sawada H,Yamahama Y,Takikawa S,Shintaku H,Hara A,Mase K,Kondo T,Iino T

更新日期：2009-07-01 00:00:00

abstract：:We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole...

journal_title：Journal of biochemistry

authors： Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo T

更新日期：2007-04-01 00:00:00

abstract：:8-Azido GTP (8-N3 GTP) was demonstrated to be polymerized into RNA by influenza virus-associated RNA polymerase at about one tenth the rate of GTP incorporation. The Km value for the azido analogue of GTP in primer-dependent RNA synthesis was 94 microM whereas Km for the natural substrate, GTP, was 6.7 microM. Upon ex...

journal_title：Journal of biochemistry

authors： Asano Y,Mizumoto K,Maruyama T,Ishihama A

更新日期：1995-03-01 00:00:00

abstract：:To analyze the processing of extracellular enzymes of Bacillus subtilis, an NH2-terminally extended hybrid alpha-amylase [pTUBE638-alpha-amylase (E24)] was purified from the periplasm of E. coli(pTUBE638) as the substrate for the in vitro processing reaction, in which a 21-amino-acid extra-peptide was added at the NH2...

journal_title：Journal of biochemistry

authors： Itoh Y,Sumi M,Nakamura K,Yamane K

更新日期：1990-12-01 00:00:00

abstract：:Deoxyhypusine synthase catalyzes the NAD+-dependent formation of deoxyhypusine in the eIF-5A precursor protein by transferring the 4-aminobutyl moiety of spermidine. This enzyme has recently been shown to be essential for cell viability and growth of yeast [Sasaki, K., Abid, M.R., and Miyazaki, M. (1996) FEBS Lett. 38...

journal_title：Journal of biochemistry

authors： Abid MR,Sasaki K,Titani K,Miyazaki M

更新日期：1997-04-01 00:00:00

abstract：:The rate of slow Li+ influx and the fraction of active form of acetylcholine receptor (AChR) of Electrophorus electricus membrane vesicles at equilibrium between the active and desensitized forms of the receptor were measured in the presence of various concentrations of phenyltrimethylammonium (PTA) and nereistoxin (N...

journal_title：Journal of biochemistry

authors： Aoshima H,Hori K,Yamamoto A

更新日期：1987-02-01 00:00:00

abstract：:The molecular masses of two of the four DNA polymerase alpha-primase complex subunit peptides from various mammalian cells have been compared through the use of specific monoclonal antibodies. One monoclonal antibody (E4) binds to 77-kDa peptide from HeLa cells and cognate peptides from other mammalian cells (monkey, ...

journal_title：Journal of biochemistry

authors： Kozu T,Nikaido K,Yagura T

更新日期：1990-04-01 00:00:00

abstract：:Capacitative Ca(2+) entry is essential for refilling intracellular Ca(2+) stores and is thought to be regulated primarily by inositol 1, 4,5-trisphosphate (IP(3))-sensitive stores in nonexcitable cells. In nonexcitable A549 cells, the application of caffeine or ryanodine induces Ca(2+) release in the absence of extrac...

journal_title：Journal of biochemistry

authors： Xue HH,Zhao DM,Suda T,Uchida C,Oda T,Chida K,Ichiyama A,Nakamura H

更新日期：2000-08-01 00:00:00

abstract：:Ubiquinone was biosynthesized when rat liver mitochondria were incubated with S-adenosyl-L-methionine, solanesyl diphosphate, and [U-14C]p-hydroxybenzoate. The intermediates of ubiquinone biosynthesis but not ubiquinone were accumulated in mitochondria incubated without S-adenosyl-L-methionine and the accumulated inte...

journal_title：Journal of biochemistry

authors： Kang D,Fujiwara T,Takeshige K

更新日期：1992-03-01 00:00:00

abstract：:Mitochondrial processing peptidase (MPP), a metalloendopeptidase consisting of alpha and beta subunits, specifically recognizes a large variety of mitochondrial precursor proteins and cleaves off the N-terminal basic matrix targeting signals (MTS). Basic residues in MTS and acidic sites in MPP are required for effecti...

journal_title：Journal of biochemistry

authors： Kitada S,Ito A

更新日期：2001-01-01 00:00:00

abstract：:Using mutant strain ABYS1 of Saccharomyces cerevisiae lacking four main vacuolar proteinases, proteinase A, proteinase B, carboxypeptidase Y, and carboxypeptidase S, we examined the identities of chromatin-associated proteinases, ruling out possible contamination of the chromatin fraction by them. The chromatin of str...

journal_title：Journal of biochemistry

authors： Motizuki M,Kohno H,Tsurugi K

更新日期：1988-08-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:The heavy enzyme of gramicidin S synthetase was purified to an almost homogeneous state by a combination of ammonium sulfate fractionation, ornithine-Sepharose 4B chromatography, DEAE-cellulose chromatography, and Ultrogel AcA 22 chromatography. The enzyme was proved to be essentially homogeneous by ultracentrifugatio...

journal_title：Journal of biochemistry

authors： Hori K,Kurotsu T,Kanda M,Miura S,Nozoe A,Saito Y

更新日期：1978-08-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1...

journal_title：Journal of biochemistry

authors： Fukumori F,Takeuchi N,Hagiwara T,Ohbayashi H,Endo T,Kochibe N,Nagata Y,Kobata A

更新日期：1990-02-01 00:00:00

abstract：:We previously reported that internucleosomal DNA fragmentation, a biochemical feature of apoptosis, was induced spontaneously in high-cell-density culture of adult rat hepatocytes. To understand better the intracellular mechanism of the DNA fragmentation in this system, we have examined the effects of several inhibito...

journal_title：Journal of biochemistry

authors： Maeda S,Suzuki A,Lin KH,Inagaki H,Saito T

更新日期：1995-12-01 00:00:00

abstract：:The effects of various divalent cations on the Ca2+ uptake by microsomes from bovine aortic smooth muscle were studied. High concentrations (1 mM) of Co2+, Zn2+, Mn2+, Fe2+, and Ni2+ inhibited neither the Ca2+ uptake by the microsomes nor the formation of the phosphorylated intermediate (E approximately P) of the Ca2+...

journal_title：Journal of biochemistry

authors： Sumida M,Hamada M,Takenaka H,Hirata Y,Nishigauchi K,Okuda H

更新日期：1986-09-01 00:00:00

abstract：:1. Fractionation of fibroin prepared from the posterior silk glands of Bombyx mori was carried out. After carboxymethylation of the fibroin, it was fractionated by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-cellulose column chromatography. 2. The fibroin was composed of at least two protein...

journal_title：Journal of biochemistry

authors： Shimura K,Kikuchi A,Ohtomo K,Katagata Y,Hyodo A

更新日期：1976-10-01 00:00:00

abstract：:The P1' Ser(50) at the second reactive site of soybean protease inhibitor C-II was replaced with arginine to confirm the contribution of this residue to the inhibition. The Arg derivative had less trypsin inhibitory activity (Ki = 1 X 10(-7) M) than the Ser derivative (Ki = 2 X 10(-8) M), in contrast to the results ob...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Teshima T,Ikenaka T

更新日期：1987-09-01 00:00:00

abstract：:High mobility group box 1 (HMGB1) protein is the most abundant chromatin-associated non-histone protein expressed in all nucleated eukaryotic cells. We examined the phosphorylation of mammalian HMGB1 by testing the ability of the cyclin-dependent kinase 5 (Cdk5) to use as substrates native protein, either unmodified o...

journal_title：Journal of biochemistry

authors： Ugrinova I,Pashev IG,Pasheva EA

更新日期：2011-05-01 00:00:00

abstract：:The regulation of neutral cytosolic cholesterol ester hydrolase was studied in isolated rat liver cells. Addition of glucagon to cell suspensions caused a decrease in the enzyme activity which was significant at 1 nM concentration. The cyclic nucleotide analogue bibutyryl cyclic AMP (10 and 100 microM) also inhibited ...

journal_title：Journal of biochemistry

authors： Ruiz MB,Ochoa B,Lacort M

更新日期：1990-03-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:Type A atrial natriuretic peptide (ANP) receptor was demonstrated to be present as a tetramer in the bovine adrenal cortex. Type A ANP receptor is composed of two functional domains, namely extracellular ANP-binding and cytoplasmic guanylate cyclase domains, and generally considered to be present as a single polypepti...

journal_title：Journal of biochemistry

authors： Iwata T,Uchida-Mizuno K,Katafuchi T,Ito T,Hagiwara H,Hirose S

更新日期：1991-07-01 00:00:00

abstract：:The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...

journal_title：Journal of biochemistry

authors： Kohno K

更新日期：2010-01-01 00:00:00

abstract：:The effect of ryanodine on the permeation of glucose, choline, and Ca2+ through the Ca2+ channel in sarcoplasmic reticulum vesicles was studied by means of the light scattering and/or the tracer method. Ryanodine had the same effect on the permeabilities of choline and Ca2+; low concentrations of ryanodine (10 microM)...

journal_title：Journal of biochemistry

authors： Kasai M,Kawasaki T

更新日期：1993-03-01 00:00:00

abstract：:We investigated the significance of the plasma membrane lipid composition in exocytosis in an in vitro interaction system using an intact secretory granular fraction (SG) isolated from the rat parotid gland. When various liposomes (as a model of plasma membranes) were added to this assay system, rapid and transient am...

journal_title：Journal of biochemistry

authors： Mizuno-Kamiya M,Inokuchi H,Kameyama Y,Yashiro K,Shin SO,Fujita A

更新日期：1995-10-01 00:00:00

abstract：:Galactocerebrosidase was purified about 22,600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 x 10(5) nmol/h/mg with tritium-labeled galactocerebroside as the substrate in the taurocholate system. The optimal pH for galactocer...

journal_title：Journal of biochemistry

authors： Sakai N,Inui K,Midorikawa M,Okuno Y,Ueda S,Iwamatsu A,Okada S

更新日期：1994-09-01 00:00:00