Abstract：
:Capacitative Ca(2+) entry is essential for refilling intracellular Ca(2+) stores and is thought to be regulated primarily by inositol 1, 4,5-trisphosphate (IP(3))-sensitive stores in nonexcitable cells. In nonexcitable A549 cells, the application of caffeine or ryanodine induces Ca(2+) release in the absence of extracellular Ca(2+) similar to that induced by thapsigargin (Tg), and Ca(2+) entry occurs upon the readdition of extracellular Ca(2+). The channels thus activated are also permeable to Mn(2+). The channels responsible for this effect appear to be activated by the depletion of caffeine/ryanodine-sensitive stores per se, as evidenced by the activation even in the absence of increased intracellular Ca(2+) concentration. Tg pretreatment abrogates the response to caffeine/ryanodine, whereas Tg application subsequent to caffeine/ryanodine treatment induces further Ca(2+) release. The response to caffeine/ryanodine is also abolished by initial ATP application, whereas ATP added subsequent to caffeine/ryanodine induces additional Ca(2+) release. RT-PCR analyses showed the expression of a type 1 ryanodine receptor, two human homologues of transient receptor potential protein (hTrp1 and hTrp6), as well as all three types of the IP(3) receptor. These results suggest that in A549 cells, (i) capacitative Ca(2+) entry can also be regulated by caffeine/ryanodine-sensitive stores, and (ii) the RyR-gated stores interact functionally with those sensitive to IP(3), probably via Ca(2+)-induced Ca(2+) release.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Xue HH,Zhao DM,Suda T,Uchida C,Oda T,Chida K,Ichiyama A,Nakamura Hdoi
10.1093/oxfordjournals.jbchem.a022757keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
329-36issue
2eissn
0021-924Xissn
1756-2651journal_volume
128pub_type
杂志文章abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124073
更新日期：1993-04-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a133858
更新日期：1982-05-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/101.3.789
更新日期：1987-03-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021960
更新日期：1998-03-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124569
更新日期：1994-09-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123958
更新日期：1992-11-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a121734
更新日期：1986-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021811
更新日期：1997-10-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvn054
更新日期：2008-08-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134462
更新日期：1983-10-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122858
更新日期：1989-09-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvn143
更新日期：2009-01-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvi080
更新日期：2005-06-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a133716
更新日期：1982-02-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124688
更新日期：1994-12-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvg152
更新日期：2003-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022379
更新日期：1999-06-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124762
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvh140
更新日期：2004-10-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134045
更新日期：1982-10-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a135094
更新日期：1985-02-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134816
更新日期：1984-07-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a130737
更新日期：1975-02-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132368
更新日期：1979-02-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124110
更新日期：1993-06-01 00:00:00
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journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124617
更新日期：1994-10-01 00:00:00
abstract：:Histidinoalanine, a cross-linking component of connective tissue proteins, was detected in the acid hydrolysate of human urine. The concentrations in urines from newborn babies, children, and adults were 1.33 +/- 0.27, 0.77 +/- 0.23, and 0.89 +/- 0.33 nmol/mg creatinine, respectively. Possible origins of urinary histi...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a121774
更新日期：1986-09-01 00:00:00
abstract：:A sensitive enzyme-immunochemical staining method was developed for detection of N-glycolylneuraminic acid (NeuGc)-containing glycosphingolipids (GSLs) on silica gel thin-layer chromatography. The procedure consists of immune reaction among NeuGc-containing GSLs, affinity-purified chicken anti-NeuGc-LacCer and horsera...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134760
更新日期：1984-05-01 00:00:00
