Studies on cellulases of a phytopathogenic fungus, Pyricularia oryzae Cavara. III. Multiplicity of beta-glucosidase, and purification and properties of a second component.

abstract：:Increased fructose concentration in erythrocytes of diabetic patients subject haemoglobin (Hb) to be glycated by fructose. Haemoglobin glycation results in early and advanced glycation end products which are known as HbA(1c) and Hb-AGE, respectively. In diabetics the Hb-AGE content raises and the mean parameter multip...

journal_title：Journal of biochemistry

authors： Bakhti M,Habibi-Rezaei M,Moosavi-Movahedi AA,Khazaei MR

更新日期：2007-06-01 00:00:00

abstract：:20S proteasomes from higher eukaryotes have immunological functions rather than those from archibacteria or yeast. To clarify the mechanism of the sorting and production of antigen-presenting peptides, it is important and worthwhile to determine the structure of mammalian proteasomes using a third generation synchrotr...

journal_title：Journal of biochemistry

authors： Tomisugi Y,Unno M,Mizushima T,Morimoto Y,Tanahashi N,Tanaka K,Tsukihara T,Yasuoka N

更新日期：2000-06-01 00:00:00

abstract：:Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV...

journal_title：Journal of biochemistry

authors： Asano K,Suzuki M,Handa S,Oshima M

更新日期：1991-01-01 00:00:00

abstract：:The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

journal_title：Journal of biochemistry

authors： Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

更新日期：1993-05-01 00:00:00

abstract：:The activity of thermolysin is greatly enhanced in the presence of high concentrations of neutral salts [Holmquist, B. and Vallee, B.L. (1976) Biochemistry 15, 101-107; Inouye, K. (1992) J. Biochem. 112, 335-340]. NaBr and NaCl are the most effective for the activation. An absorption difference spectrum with a peak ar...

journal_title：Journal of biochemistry

authors： Inouye K,Kuzuya K,Tonomura B

更新日期：1994-09-01 00:00:00

abstract：:Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actin. The kinetics of depolymerization suggest that aplyronine A...

journal_title：Journal of biochemistry

authors： Saito S,Watabe S,Ozaki H,Kigoshi H,Yamada K,Fusetani N,Karaki H

更新日期：1996-09-01 00:00:00

abstract：:GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

更新日期：1983-03-01 00:00:00

abstract：:Mitochondrial DNA of an erythromycin-resistant petite mutant of yeast, E734, showed physical maps of inversion, which occurred between two cross-over sites in the fragments Hae A and Hae B. The pair of cross-over sites was inferred to be accommodated within a repeat unit of E734 mtDNA during petite mutation by joining...

journal_title：Journal of biochemistry

authors： Wakabayashi K,Furutani Y

更新日期：1984-11-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:To understand the molecular basis of the thermostability of a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1, we introduced mutations at Pro5, Pro7, Pro240 and Pro268, which are located on the surface loops of aqualysin I, by changing these amino acid residues into those found at the correspondin...

journal_title：Journal of biochemistry

authors： Sakaguchi M,Matsuzaki M,Niimiya K,Seino J,Sugahara Y,Kawakita M

更新日期：2007-02-01 00:00:00

abstract：:The gizzard tropomyosin molecule is composed of two subunits at 1:1 molar ratio. Possible composites of the tropomyosin molecule are two kinds of homodimer (one for each subunit), a heterodimer of two subunits, or a mixture of heterodimer and homodimer(s). We tried to evaluate the native subunit composition of gizzard...

journal_title：Journal of biochemistry

authors： Nakamura S,Sakurai T,Nonomura Y

更新日期：1991-05-01 00:00:00

abstract：:The voltage-dependent L-type Ca2+ channel in the heart is regulated by cAMP-dependent protein kinase (PKA) and possibly by protein kinase C (PKC). We have investigated the channel modulation through phosphorylation by these protein kinases, using liposomes into which Ca2+ channels from bovine heart were reconstituted....

journal_title：Journal of biochemistry

authors： Kameyama A,Shearman MS,Sekiguchi K,Kameyama M

更新日期：1996-07-01 00:00:00

abstract：:Calf thymus DNA polymerases alpha and beta [EC 2.7.7.7] and terminal deoxynucleotidyl transferase [EC 2.7.7.31] were analyzed on two-dimensional gel slabs. DNA polymerase beta appeared as a single spot on two-dimensional gel at the position of 40,000 daltons and pI 8.0 using non-equilibrium pH gradient gel electrophor...

journal_title：Journal of biochemistry

authors： Koiwai O,Masaki S,Kaneda T,Yoshida S

更新日期：1982-08-01 00:00:00

abstract：:A particulate enzyme preparation from Bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from CDPglycerol at an optimum pH of 8.0 and the reaction was stimulated by divalent cations. Km for CDPglycerol was 0.18 mM. The synthesis was inhibited by CMP, CDP, and CTP and by concentrations of CDP-glycerol...

journal_title：Journal of biochemistry

authors： Cheang OO K

更新日期：1975-03-01 00:00:00

abstract：:A simple and rapid preparation method for apoaspartate aminotransferase from Escherichia coli B was developed. A crude extract of the bacterial cells was treated batchwise with DEAE-cellulose. The enzyme fraction obtained was then applied to a pyridoxamine-Sepharose column. Apoaspartate aminotransferase was eluted wit...

journal_title：Journal of biochemistry

authors： Yagi T,Kirino J,Yamamoto S,Nozaki M

更新日期：1985-10-01 00:00:00

abstract：:Droplet counter-current chromatography (DCC) a novel liquid-liquid partition technique, is a useful method for the separation of glycolipids. After a series of investigations on suitable experimental conditions for DDC, mouse brain chloroform-methanol-0.5% CaCl2-n-propanol (50:60:40:6) by the ascending method with 500...

journal_title：Journal of biochemistry

authors： Otsuka H,Yamakawa T

更新日期：1981-07-01 00:00:00

abstract：:Diacylglycerol lipase (DGL) was solubilized from human platelet microsomes with heptyl-beta-D-thioglucoside, and purified to homogeneity on SDS-PAGE using a combination of chromatographic and electrophoretic methods. The molecular mass of the purified DGL was estimated to be 33 kDa. Its apparent pI was pH 6.0, as dete...

journal_title：Journal of biochemistry

authors： Moriyama T,Urade R,Kito M

更新日期：1999-06-01 00:00:00

abstract：:1. The light-induced pH change of chromatophore suspensions from Rhodospirillum rubrum was stimulated significantly and similarly by KCl, NaCl, LiCl, RbCl, CsCl, MgCl2, MnCl2, and CaCl2. In the dark, the pH of chromatophore suspensions decreased immediately and markedly on adding these salts. 2. The light-induced pH c...

journal_title：Journal of biochemistry

authors： Nishi N,Sakata-Sogawa K,Soe G,Yamashita J

更新日期：1977-11-01 00:00:00

abstract：:Elafin was shown to be a new type of proteinase inhibitor which has an anchoring sequence. Human elafin, a potent inhibitor specific for elastase and proteinase 3, has a unique repeating sequence in its prosegment that is rich in Gln and Lys residues. The prosegment, termed "cementoin," exhibits high homology with the...

journal_title：Journal of biochemistry

authors： Nara K,Ito S,Ito T,Suzuki Y,Ghoneim MA,Tachibana S,Hirose S

更新日期：1994-03-01 00:00:00

abstract：:pKY2289, a ColE1::Tn3 derivative, useful for direct selection of cells carrying a hybrid plasmid, was deleted of its mobility functions and parts of the transposon including the left inverted repeat. This deletion mutant, named pKY2700 expresses low levels of colicin E1 synthesis even in recA cells. A nitrosoguanidine...

journal_title：Journal of biochemistry

authors： Ozaki LS,Kimura A,Shimada K,Takagi Y

更新日期：1982-04-01 00:00:00

abstract：:A number of gene mutations are detected in cells derived from human cancer tissues, but roles of these mutations in cancer cell development are largely unknown. We examined G364R mutation of MCM4 detected in human skin cancer cells. Formation of MCM4/6/7 complex is not affected by the mutation. Consistent with this no...

journal_title：Journal of biochemistry

authors： Ishimi Y,Irie D

更新日期：2015-06-01 00:00:00

abstract：:The interaction between lipopolysaccharide (LPS) and an LPS-sensitive serine protease zymogen, factor C, purified from horseshoe crab (Tachypleus tridentatus) hemocytes, was investigated to elucidate the LPS-mediated activation of factor C. The rate of activation of the zymogen factor C was highly dependent on the con...

journal_title：Journal of biochemistry

authors： Nakamura T,Tokunaga F,Morita T,Iwanaga S

更新日期：1988-02-01 00:00:00

abstract：:The heavy chain of myosin subfragment-1 prepared by chymotrypsin treatment had a molecular weight of about 96 K. It was split into 26 K, 50K, and 21 K fragments on trypsin treatment. The effect of actin binding on the susceptibilities of the junctions between 26 K and 50 K and between 50 K and 21 K, and on that of alk...

journal_title：Journal of biochemistry

authors： Yamamoto K,Sekine T

更新日期：1979-12-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00

abstract：:We previously reported a new laminin variant containing laminin gamma 2 (or B2t) chain, ladsin, which exerted prominent cell-scattering, cell-adhesion, and cell-migration activities. In the present study, this laminin was further characterized, and gene expression of its three subunits in various human tissues and can...

journal_title：Journal of biochemistry

authors： Mizushima H,Miyagi Y,Kikkawa Y,Yamanaka N,Yasumitsu H,Misugi K,Miyazaki K

更新日期：1996-12-01 00:00:00

abstract：:To investigate the fine substrate specificities of four highly purified exo-type cellulases (Exo-A from Aspergillus niger, CBHI and CBHII from Trichoderma reesei, and Ex-1 from Irpex lacteus), water-soluble substrates such as barley glucan, xyloglucan from tamarind (Tamarindus indica L.), and their oligosaccharides we...

journal_title：Journal of biochemistry

authors： Amano Y,Shiroishi M,Nisizawa K,Hoshino E,Kanda T

更新日期：1996-12-01 00:00:00

abstract：:ADAMTS13 is the metalloprotease responsible for the proteolytic degradation of von Willebrand factor (VWF). A severe deficiency of this VWF-cleaving protease activity causes thrombotic thrombocytopenic purpura. This protease, comprising 1,427 amino acid residues, is composed of multiple domains, i.e., a preproregion, ...

journal_title：Journal of biochemistry

authors： Soejima K,Nakamura H,Hirashima M,Morikawa W,Nozaki C,Nakagaki T

更新日期：2006-01-01 00:00:00

abstract：:In order to stabilize human RNase 1 by introduction of an intramolecular cross-link, a mutant protein (4-118CL RNase 1), in which Arg4 and Val118 are replaced with cysteine residues and linked by a disulfide bond, was designed and expressed in Escherichia coli as inclusion bodies. The 4-118CL RNase 1 that refolded und...

journal_title：Journal of biochemistry

authors： Futami J,Tada H,Seno M,Ishikami S,Yamada H

更新日期：2000-08-01 00:00:00

abstract：:The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...

journal_title：Journal of biochemistry

authors： Kikuchi K,Umehara T,Fukuda K,Hwang J,Kuno A,Hasegawa T,Nishikawa S

更新日期：2003-03-01 00:00:00