Analysis on the molecular species and concentration of circulating ADAMTS13 in Blood.

abstract：:Hepatitis C virus (HCV) core protein has been suggested to play crucial roles in the pathogeneses of liver steatosis and hepatocellular carcinomas due to HCV infection. Intracellular HCV core protein is localized mainly in lipid droplets, in which the core protein should exert its significant biological/pathological f...

journal_title：Journal of biochemistry

authors： Sato S,Fukasawa M,Yamakawa Y,Natsume T,Suzuki T,Shoji I,Aizaki H,Miyamura T,Nishijima M

更新日期：2006-05-01 00:00:00

abstract：:Detergent treatments were examined for their efficacy in purifying trimethylamine-N-oxide (TMAO) demethylase activity from fish muscle microsomes. Tritons X-100 and X-45, deoxycholate, Brijs, Tweens 20, 65, and 80, and SDS were generally ineffective in solubilizing demethylase activity from this membrane fraction, at ...

journal_title：Journal of biochemistry

authors： Parkin KL,Hultin HO

更新日期：1986-07-01 00:00:00

abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...

journal_title：Journal of biochemistry

authors： Poetsch A,Berzborn RJ,Heberle J,Link TA,Dencher NA,Seelert H

更新日期：2007-03-01 00:00:00

abstract：:A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

journal_title：Journal of biochemistry

authors： Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

更新日期：1983-12-01 00:00:00

abstract：:The plant hormone ethylene is generated from a unique precursor, 1-aminocyclopropane-1-carboxylate (ACC). In previous studies, ACC deaminase, which degrades ACC to alpha-ketobutyrate and ammonia, was found in four strains of Pseudomonas, characterized, and sequenced. To verify the wider distribution of ACC deaminase i...

journal_title：Journal of biochemistry

authors： Minami R,Uchiyama K,Murakami T,Kawai J,Mikami K,Yamada T,Yokoi D,Ito H,Matsui H,Honma M

更新日期：1998-06-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:A large COOH-terminal domain of Serratia marcescens serine protease (SSP) is essentially required for the excretion of the mature protease region through the outer membrane in Escherichia coli. For investigation of the possibility of transporting foreign periplasmic proteins by utilizing the function of the COOH-termi...

journal_title：Journal of biochemistry

authors： Shimada K,Ohnishi Y,Horinouchi S,Beppu T

更新日期：1994-08-01 00:00:00

abstract：:We previously reported that novel Mg(2+)-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associat...

journal_title：Journal of biochemistry

authors： Shimizu S,Imanaka T,Takano T,Ohkuma S

更新日期：1992-12-01 00:00:00

abstract：:In our recent study on monoclonal antibodies (mAbs AHA-1-5) against glutaraldehyde (GA)-conjugated histamine (HA), we identified one mAb (AHA-2) which can detect neuronal HA in the rat brain with an immunocytochemistry method (ICC) [Fujiwara et al. (1999) J. Biochem. 126, 503-509]. In the present study the specificity...

journal_title：Journal of biochemistry

authors： Fujiwara K,Murata I,Yagisawa S,Tanabe T,Yabuuchi M,Sakakibara R,Tsuru D

更新日期：1999-12-01 00:00:00

abstract：:Human matrix metalloproteinase 7 (MMP-7) activity exhibits broad bell-shaped pH profile with the acidic and alkaline pK(a) (pK(e1) and pK(e2)) values of about 4 and 10. The ionizable group for pK(e2) was assigned to Lys or Arg by thermodynamic analysis; however, no such residues are present in the active site. Hence, ...

journal_title：Journal of biochemistry

authors： Morishima A,Yasukawa K,Inouye K

更新日期：2012-05-01 00:00:00

abstract：:A new hetero-bifunctional photo crosslinking reagent, 2-(4-azidoanilyl)-4-(4-azabicyclo-[2,2, 2]hexylammonio)-6-morpholino-1,3,5-triazine chloride, was designed to detect and isolate heparin-binding protein(s) that may act as heparin-receptor(s) on the platelet surface. In a preliminary study using ethanol as a model ...

journal_title：Journal of biochemistry

authors： Suda Y,Mori K,Bird K,Marques D,Ormsby J,Tanaka S,Koshida S,Nakamura M,Kusumoto S,Sobel M

更新日期：1999-06-01 00:00:00

abstract：:Affinity labeling with nucleoside polyphosphopyridoxals, especially those with 3 or 4 phosphate groups, was effective for identifying the lysyl residue(s) located at or near the binding site for ATP, GTP, UDP-Glc, or ADP-Glc in various proteins. Furthermore, kinetic analysis of the mutant enzymes, in which the labeled...

journal_title：Journal of biochemistry

authors： Fukui T

更新日期：1995-06-01 00:00:00

abstract：:DNA synthesis in the Bacillus subtilis cells has become possible using extra-cellular DNA. Generally, purified DNAs in a test tube have been required to introduce into the host cells for molecular cloning technology in the laboratory. We have developed a cell lysis technique for natural transformation using stable ext...

journal_title：Journal of biochemistry

authors： Kaneko S,Fukushima H,Nakahama M,Asano S,Miyazaki Y,Aizawa Y,Itaya M

更新日期：2018-04-01 00:00:00

abstract：:L-Glutamate dehydrogenase (GLDH) independent of NAD(P) and oxygen was first obtained from the psychrotrophic bacterium Aeromonas sp. L101, originally isolated from the organs of salmon (Oncorhynchus keta). GLDH was purified by a series of chromatography steps on DEAE-Sepharose, Superdex 200pg, Q-Sepharose, CM-Sepharos...

journal_title：Journal of biochemistry

authors： Yamamura A,Sakaguchi T,Murakami Y,Yokoyama K,Tamiya E

更新日期：1999-04-01 00:00:00

abstract：:Plant cell-wall arabinoxylans have a complex structure that requires the action of a pool of debranching (arabinofuranosidases) and depolymerizing enzymes (endo-xylanase). Two Aspergillus nidulans strains over-secreting endo-xylanase and arabinofuranosidase were inoculated in defined 2% maltose-minimum medium resultin...

journal_title：Journal of biochemistry

authors： Damásio AR,Pessela BC,da Silva TM,Guimarães LH,Jorge JA,Guisán JM,Polizeli Mde L

更新日期：2013-09-01 00:00:00

abstract：:The Serratia marcescens serine protease gene encoding a 1,045-amino-acid precursor protein of 112 kDa directs excretion of the mature protease of ca. 58 kDa through the outer membrane of Escherichia coli. A typical signal peptide of 27 amino acids and a large COOH-terminal domain of the precursor are both functionally...

journal_title：Journal of biochemistry

authors： Shikata S,Shimada K,Kataoka H,Horinouchi S,Beppu T

更新日期：1992-05-01 00:00:00

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00

abstract：:Studies on the solution structure dynamics of RNA/DNA are becoming crucially important. The phenomena of SSCP (single-strand conformation polymorphism), small RNA dynamics in a cell, and others can be related to the conformational changes of single-stranded (ss) RNAs/DNAs in solution. However, little is known about th...

journal_title：Journal of biochemistry

authors： Biyani M,Nishigaki K

更新日期：2005-10-01 00:00:00

abstract：:We examined the effects of growth factors on intracellular free Mg2+ concentrations ([Mg2+]i) in single Swiss 3T3 fibroblasts, using microfluorometry of a Mg(2+)-sensitive dye mag-fura-2. We had already noted an increase in [Mg2+]i after exposure to bombesin for 30-60 min [Ishijima, S., Sonoda, T., & Tatibana, M. (199...

journal_title：Journal of biochemistry

authors： Ishijima S,Tatibana M

更新日期：1994-04-01 00:00:00

abstract：:Oligosaccharides of a non-oligomannoside type were released from porcine alpha-mannosidase by hydrazinolysis, and were fractionated into at least 15 homogeneous oligosaccharides. Most of them are oligosaccharides with galactose and N-acetylglucosamine residues attached to a common core, alpha Man2 beta Man beta GlcNAc...

journal_title：Journal of biochemistry

authors： Kozutsumi Y,Kawasaki T,Yamashina I

更新日期：1986-08-01 00:00:00

abstract：:In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

journal_title：Journal of biochemistry

authors： Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

更新日期：1987-08-01 00:00:00

abstract：:We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 m...

journal_title：Journal of biochemistry

authors： Nagino K,Nomura O,Takii Y,Myomoto A,Ichikawa M,Nakamura F,Higasa M,Akiyama H,Nobumasa H,Shiojima S,Tsujimoto G

更新日期：2006-04-01 00:00:00

abstract：:tRNA(guanosine-2'-)-methyltransferases (Gm-methylases) isolated from extreme thermophiles, Thermus thermophilus strains HB 27 and HB 8, methylate the 2'-OH of the G18 ribose of the GG sequence in the D loop of tRNA, by recognizing the D "loop-stem" structure as a minimal requirement. To examine the role of the consens...

journal_title：Journal of biochemistry

authors： Hori H,Saneyoshi M,Kumagai I,Miura K,Watanabe K

更新日期：1989-11-01 00:00:00

abstract：:Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its cytosolically exposed domain, is expressed in Saccharomyces cerevisiae. Using various KAR2 promoter constructs in front of ...

journal_title：Journal of biochemistry

authors： Zimmer T,Ogura A,Ohta A,Takagi M

更新日期：1999-12-01 00:00:00

abstract：:We identified a protein with a molecular weight of about 44 kDa in an extract of rat regenerating liver. This protein was undetectable in normal, sham-operated, and completely regenerated liver. We also purified this 44-kDa protein from an extract of rat liver remnant after partial hepatectomy. The partial amino acid ...

journal_title：Journal of biochemistry

authors： Tanahashi T,Suzuki M,Itoh N,Mitsui Y

更新日期：1995-08-01 00:00:00

abstract：:When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

journal_title：Journal of biochemistry

authors： Bukhtiyarov YE,Shabalin YA,Kulaev IS

更新日期：1993-06-01 00:00:00

abstract：:After partial chromatographic purification of rat liver cell sap on DEAE-cellulose, including the removal of type M2 pyruvate kinase, different forms of type L pyruvate kinase were separated by chromatofocusing. Three fractions of pyruvate kinase activity were found, eluting at pH 5.0, 5.2, and 5.3, respectively. The ...

journal_title：Journal of biochemistry

authors： Ekdahl KN,Ekman P

更新日期：1984-04-01 00:00:00

abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

journal_title：Journal of biochemistry

authors： Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

更新日期：2002-02-01 00:00:00

abstract：:Identification of single-stranded regions in Torulopsis utilis 5S RNA was attempted by the use of Nuclease S1, a single-strand specific endonuclease. When T. utilis 5S RNA was subjected to prolonged incubation with Nuclease S1, about 50% of the substrate 5S RNA remained as large oligonucleotide "cores." Such Nuclease...

journal_title：Journal of biochemistry

authors： Nishikawa K,Takemura S

更新日期：1977-04-01 00:00:00

abstract：:Purified nuclease TT1 from Thermus thermophilus HB8 has multimolecular weight forms, each of which is composed of three different subunits, alpha (10.8 x 10(4)), beta (7.8 x 10(4)), and gamma (4.1 x 10(4)). The molecular weights of this enzyme were estimated by gel filtration, polyacrylamide gel electrophoresis and eq...

journal_title：Journal of biochemistry

authors： Takahashi M,Kobayashi M,Uchida T

更新日期：1981-11-01 00:00:00