Abstract:
:Purified nuclease TT1 from Thermus thermophilus HB8 has multimolecular weight forms, each of which is composed of three different subunits, alpha (10.8 x 10(4)), beta (7.8 x 10(4)), and gamma (4.1 x 10(4)). The molecular weights of this enzyme were estimated by gel filtration, polyacrylamide gel electrophoresis and equilibrium sedimentation. It was found that most of the enzyme has a molecular weight of about 22 x 10(4) being a monomer having the subunit composition of alpha beta gamma. The remaining part of the enzyme has larger molecular weights and is considered to be size-isomers of alpha beta gamma. The alpha-helical content, 5.5--6.5%, and the beta-structure, about 28%, were estimated from the CD spectrum at 4 degrees C.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Takahashi M,Kobayashi M,Uchida Tdoi
10.1093/oxfordjournals.jbchem.a133619subject
Has Abstractpub_date
1981-11-01 00:00:00pages
1521-7issue
5eissn
0021-924Xissn
1756-2651journal_volume
90pub_type
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