The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity.

abstract：:Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

journal_title：Journal of biochemistry

authors： Murase T,Yamada N,Akanuma Y,Ohsawa N

更新日期：1982-09-01 00:00:00

abstract：:Syk protein-tyrosine kinase (PTK) has been implicated in a variety of hematopoietic cell responses including immunoreceptor signaling. However, so far, there has been no evidence of the expression of Syk or Syk-related PTK in non-hematopoietic tissues. In this study, we have purified from blood cell-depleted rat liver...

journal_title：Journal of biochemistry

authors： Tsuchida S,Yanagi S,Inatome R,Ding J,Hermann P,Tsujimura T,Matsui N,Yamamura H

更新日期：2000-02-01 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...

journal_title：Journal of biochemistry

authors： Karasaki Y,Ohno M

更新日期：1978-09-01 00:00:00

abstract：:Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin,...

journal_title：Journal of biochemistry

authors： Murayama T,Nakauchi Y,Kimura S,Maruyama K

更新日期：1989-02-01 00:00:00

abstract：:We have been interested in partially folded proteins with marginal stability and activity, because they have a potential to be mature proteins by artificial evolution. A module is defined as a contiguous peptide chain forming a compact region in a globular protein. Modules may be used as building blocks to create part...

journal_title：Journal of biochemistry

authors： Tsuji T,Nagata T,Yanagawa H

更新日期：2008-10-01 00:00:00

abstract：:We have recently demonstrated the localization of associated m-calpain and calpastatin in the endoplasmic reticulum (ER) of bovine pulmonary artery smooth muscle. Herein, we sought to determine the role of m-calpain on calcium-dependent proteolytic cleavage of Na(+)/Ca(2+) exchanger (NCX) in the ER. Treatment of the E...

journal_title：Journal of biochemistry

authors： Samanta K,Kar P,Chakraborti T,Chakraborti S

更新日期：2010-02-01 00:00:00

abstract：:Ion-exchange column-purified I-protein was labeled by fluorescein isothiocyanate (FITC) at an equimolar ratio. When FITC-labeled I-protein was reacted with glycerinated myofibrils of chicken breast muscle in a phosphate-buffered saline, fluorescence was observed at the A-band and/or the Z-line of the sarcomere. Howeve...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1988-02-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:Apoprotein of electron-transferring flavoprotein (ETF) reacts with FAD as follows: A*<-->A, A+FAD<-->holoETF. Two different forms of apoETF (A* and A) convert into each other and only one of them, A, can associate with FAD [Sato, K. et al. (1991) J. Biochem. 109, 734-740]. In the present study, the reactions between a...

journal_title：Journal of biochemistry

authors： Sato K,Nishina Y,Shiga K

更新日期：1992-12-01 00:00:00

abstract：:The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

journal_title：Journal of biochemistry

authors： Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

更新日期：2001-08-01 00:00:00

abstract：:We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

journal_title：Journal of biochemistry

authors： Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

更新日期：2011-09-01 00:00:00

abstract：:Thioredoxin 1 (Trx1) is known to play an important role in protecting against cell death. However, the mechanism for control of Trx1 in cell death resulting from DNA damage has not been fully investigated. In this study, we used the DNA-damaging agent methyl methanesulfonate (MMS) to investigate the protective effects...

journal_title：Journal of biochemistry

authors： Gu L,Gao W,Yang HM,Wang BB,Wang XN,Xu J,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:Ornithine transcarbamylase (OTC) is one of the urea cycle enzymes. While the chicken is a uricotelic animal, it has measurable OTC activity in its kidney. OTC activity is highly variable within and between chicken breeds. Chicken OTC may have some physiological significance because of its significant activity in the k...

journal_title：Journal of biochemistry

authors： Shimogiri T,Kono M,Mannen H,Mizutani M,Tsuji S

更新日期：1998-11-01 00:00:00

abstract：:Aspartic proteases of human malarial parasites are thought to play key roles in essential pathways of merozoite release, invasion and host cell hemoglobin degradation during the intraerythrocytic stages of their life cycle. Therefore, we have purified and characterized Plasmodium vivax aspartic protease, to determine ...

journal_title：Journal of biochemistry

authors： Sharma A,Eapen A,Subbarao SK

更新日期：2005-07-01 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...

journal_title：Journal of biochemistry

authors： Higashi K,Kirino Y

更新日期：1983-12-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00

abstract：:We investigated whether transforming growth factor (TGF)-β1 promoted epithelial-mesenchymal transition (EMT) and migration of human oral squamous cell carcinoma (hOSCC) cells. Among 6 hOSCC cell lines investigated, Smad2 phosphorylation and TGF-β target genes expression were most clearly upregulated following TGF-β1 s...

journal_title：Journal of biochemistry

authors： Saito D,Kyakumoto S,Chosa N,Ibi M,Takahashi N,Okubo N,Sawada S,Ishisaki A,Kamo M

更新日期：2013-03-01 00:00:00

abstract：:The inhibitory effects of galloyl pedunculagin (GP) and eugeniin on the phosphorylation of histone H2B by cAMP-dependent protein kinase (A-kinase) and autophosphorylation of its beta-regulatory subunit (A-kinase beta) were examined in vitro. It was found that (i) GP (ID(50) = approx. 50 nM) effectively inhibits the ac...

journal_title：Journal of biochemistry

authors： Kosuge S,Maekawa T,Saito C,Tanaka T,Kouno I,Ohtsuki K

更新日期：2001-03-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

journal_title：Journal of biochemistry

authors： Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

更新日期：1996-08-01 00:00:00

abstract：:The effects of unsaturated fatty acids on the activities of peroxisomal enzymes of Tetrahymena pyriformis were investigated. When saturated fatty acids and the corresponding unsaturated fatty acids (C18) were added to the culture medium at 0.05%, the activities of peroxisomal enzymes [fatty acyl-CoA oxidase (FAO), car...

journal_title：Journal of biochemistry

authors： Gotoh K,Takei M,Watanabe T,Suga T

更新日期：1986-10-01 00:00:00

abstract：:Bdellin-KL is a trypsin-plasmin inhibitor from Hirudo nipponia, whose N-terminal sequence was identified as a non-classical Kazal-type. A cDNA clone encoding the inhibitor was isolated by reverse transcription-PCR and 5' rapid amplification of cDNA ends. The cDNA showed an open reading frame of 155 amino acids compris...

journal_title：Journal of biochemistry

authors： Kim YH,Choi JG,Lee GM,Kang KW

更新日期：2001-09-01 00:00:00

abstract：:This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

journal_title：Journal of biochemistry

authors： Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

更新日期：2020-03-01 00:00:00

abstract：:Using a combined pharmacological and genetic approach, we have identified aa 260-280 in the C2 region as a critical factor in the catalytic function of protein kinase Calpha (PKCalpha). Progressive truncations from the N-terminus as well as selected internal deletion mutants were expressed in Saccharomyces cerevisiae ...

journal_title：Journal of biochemistry

authors： Rotenberg SA,Zhu J,Hansen H,Li XD,Sun XG,Michels CA,Riedel H

更新日期：1998-10-01 00:00:00

abstract：:In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed ...

journal_title：Journal of biochemistry

authors： Nakayama C,Saneyoshi M

更新日期：1985-05-01 00:00:00

abstract：:Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-ac...

journal_title：Journal of biochemistry

authors： Kobayashi K,Morisawa Y,Ishituka T,Ishimoto M

更新日期：1975-11-01 00:00:00

abstract：:To reveal the molecular orientation of plastocyanin (PC) on spinach thylakoid membranes, the position of Lys residues modified by acetic anhydride was compared between thylakoid-bound PC and the isolated one. Digestion of the isolated PC by a trypsin yielded a peptide map with seven spots prior to the acetylation of t...

journal_title：Journal of biochemistry

authors： Takano M,Takahashi M,Oobatake M,Asada K

更新日期：1985-11-01 00:00:00

abstract：:Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

journal_title：Journal of biochemistry

authors： Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

更新日期：1977-07-01 00:00:00

abstract：:Previous studies have shown that purified mitochondrial outer membrane is able to catalyze the transfer of sialic acid from CMP-Neu5Ac to an exogenous asialoglycoprotein acceptor, asialofetuin. Considering the heterogeneity of the glycan chains borne by this glycoprotein, an investigation of mitochondrial sialyltransf...

journal_title：Journal of biochemistry

authors： Gasnier F,Baubichon-Cortay H,Louisot P,Gateau-Roesch O

更新日期：1991-11-01 00:00:00

abstract：:Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...

journal_title：Journal of biochemistry

authors： Miwa T,Akaboshi E,Matsubara K

更新日期：1983-08-01 00:00:00