Abstract:
:The voltage-dependent L-type Ca2+ channel in the heart is regulated by cAMP-dependent protein kinase (PKA) and possibly by protein kinase C (PKC). We have investigated the channel modulation through phosphorylation by these protein kinases, using liposomes into which Ca2+ channels from bovine heart were reconstituted. Phosphorylation of the proteoliposomes with PKA increased the dihydropyridine-sensitive Ca2+ efflux from them by about 70%. PKA rapidly phosphorylated membrane proteins of 210 and 170 kDa. A dihydropyridine-class Ca2+ channel blocker, [3H]azidopine, specifically photo-labeled a protein of 210 kDa, suggesting that the 210-kDa phosphoprotein might be the alpha 1 subunit of the Ca2+ channel. In contrast, phosphorylation of the proteoliposomes with PKC failed to modulate the Ca2+ efflux. Although PKC catalyzed the phosphorylation of membrane proteins of 150, 130, 95, 67, and 62 kDa, the 210- and 170-kDa proteins were not phosphorylated by this kinase. These results suggest that phosphorylation of the 210-kDa protein in the cardiac sarcolemma by PKA may be responsible for modulation of the channel function, whereas modulation of the channel by PKC, if it occurs, must be the result of an indirect mechanism, e.g. phosphorylation of a cytoplasmic protein or an associated channel polypeptide, that cannot function in the reconstituted system.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Kameyama A,Shearman MS,Sekiguchi K,Kameyama Mdoi
10.1093/oxfordjournals.jbchem.a021380subject
Has Abstractpub_date
1996-07-01 00:00:00pages
170-6issue
1eissn
0021-924Xissn
1756-2651journal_volume
120pub_type
杂志文章abstract::Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...
journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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abstract::Increased fructose concentration in erythrocytes of diabetic patients subject haemoglobin (Hb) to be glycated by fructose. Haemoglobin glycation results in early and advanced glycation end products which are known as HbA(1c) and Hb-AGE, respectively. In diabetics the Hb-AGE content raises and the mean parameter multip...
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
abstract::Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells...
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abstract::Mitochondrial porin is a major integral membrane protein of the outer membrane. To assess the stop-transfer sequence in the yeast porin molecule (P), we constructed the following chimeric proteins. (i) The signal sequence of interleukin 2, a secretory protein, was fused to the amino-terminus of porin (SP). (ii) The ma...
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123884
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
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更新日期:1998-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134352
更新日期:1983-07-01 00:00:00
abstract::The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122292
更新日期:1988-03-01 00:00:00
abstract::The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the...
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124432
更新日期:1994-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1981-12-01 00:00:00
abstract::A beta-galactosidase was extracted from the internal organs of a sea squirt, Styela plicata, and purified 959-fold, with an 18% yield, by successive gel chromatography, anion-exchange chromatography, chromatofocusing, and affinity chromatography on a Con A-Sepharose column. The purified enzyme was fairly homogeneous, ...
journal_title:Journal of biochemistry
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doi:
更新日期:1991-07-01 00:00:00
abstract::The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
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更新日期:2020-03-01 00:00:00
abstract::Two transferrin (Tf) glycan-isoforms were previously found in cerebrospinal fluid (CSF); one appears to be derived from serum (Tf-2) and the other from choroid plexus, a CSF-producing tissue (Tf-1). To analyse metabolic differences associated with the two isoforms, their ratio (Tf-2/Tf-1) was defined as the Tf index. ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
abstract::1. The endogenous phosphorylation of mouse brain microsomes was studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). 2. It was found that specific proteins and lipids in brain microsomes were phosphorylated by the terminal phosphate of ATP under appropriate con...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131866
更新日期:1977-12-01 00:00:00
abstract::The acceptor specificity of the transglycosylation reaction of cyclodextrin glycosyltransferase[EC 2.4.1.19] was investigated using various sugars and sugar alcohols. L-Sorbose, D-xylose, and D-galactose, which contain configurational or structural changes relative to the D-glucopyranose unit at positions other than p...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1976-03-01 00:00:00
abstract::The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124827
更新日期:1995-05-01 00:00:00
abstract::Human matrix metalloproteinase 7 (MMP-7) activity exhibits broad bell-shaped pH profile with the acidic and alkaline pK(a) (pK(e1) and pK(e2)) values of about 4 and 10. The ionizable group for pK(e2) was assigned to Lys or Arg by thermodynamic analysis; however, no such residues are present in the active site. Hence, ...
journal_title:Journal of biochemistry
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更新日期:2012-05-01 00:00:00
abstract::Troponin is the regulatory protein of striated muscle. Without Ca2+, the contraction of striated muscle is inhibited. Binding of Ca2+ to troponin activates contraction. The location of troponin on the thin filaments and its relation to the regulatory mechanism has been unknown, though the Ca2+-induced dislocation of t...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022424
更新日期:1999-07-01 00:00:00