Subgroup differences in 'brain-type' transferrin and α-synuclein in Parkinson's disease and multiple system atrophy.

abstract：:Methylation and demethylation of the methyl-accepting chemotaxis protein (MCP) was studied in vitro. The in vitro MCP methylating system showed the following characteristics. 1. Multiple bands of methylated MCP were produced. 2. The reaction could be separated into two phases: the initial phase, in which the level of ...

journal_title：Journal of biochemistry

authors： Hayashi H,Minoshima S,Ohba M

更新日期：1982-08-01 00:00:00

abstract：:N omega-Phosphoarginine hydrolase from rat liver cytosol was purified to apparent homogeneity on SDS-PAGE, by employing column chromatographies on Sephadex G-75, DEAE-cellulose, QAE-Toyopearl, and glutathione-2-pyridyl-disulfide-Superose. One milligram protein of the final preparation released 4 mumol/min of inorganic...

journal_title：Journal of biochemistry

authors： Yokoyama K,Ohmori H,Kumon A

更新日期：1993-02-01 00:00:00

abstract：:The complete amino acid sequence of acylphosphatase from rabbit skeletal muscle has been elucidated by automatic Edman degradation of peptides obtained from staphylococcal protease and trypsin digestions. The enzyme consisted of a single polypeptide chain of 98 amino acid residues, lacking only histidine. Its amino (N...

journal_title：Journal of biochemistry

authors： Kizaki T,Takasawa T,Mizuno Y,Shiokawa H

更新日期：1985-04-01 00:00:00

abstract：:The effects of sphingoid bases, sphingosine and dihydrosphingosine, which are protein kinase C (PKC) inhibitors, on NADPH oxidase were examined in a cell-free system. The bases inhibited cell-free activation of NADPH oxidase by arachidonic acid at lower concentration than N-acetylsphingosine. Thus, positive charge in ...

journal_title：Journal of biochemistry

authors： Sasaki JI,Yamaguchi M,Saeki S,Yamane H,Okamura N,Ishibashi S

更新日期：1996-10-01 00:00:00

abstract：:Under conditions where nM level of calmodulin was able to show full activation of myosin light chain kinase and cyclic-nucleotide phosphodiesterase, the fragments of calmodulin at concentrations as high as 20 microM failed to activate these enzymes in the presence of Ca2+. The fragments tested were Ala1-Lys75 (F12), A...

journal_title：Journal of biochemistry

authors： Minowa O,Yazawa M,Sobue K,Ito K,Yagi K

更新日期：1988-03-01 00:00:00

abstract：:Pyruvate decarboxylase [2-oxo acid carboxy-lyase, EC 4.1.1.1] was isolated from sweet potato roots and was partially purified from healthy and diseased tissues. There was no appreciable difference in properties between the enzymes from healthy and diseased tissues. The molecular weight of the enzyme was found to be 24...

journal_title：Journal of biochemistry

authors： Oba K,Uritani I

更新日期：1975-06-01 00:00:00

abstract：:Seven ganglioside species were isolated and purified from erythrocyte membranes of Japanese cats by DEAE-Sephadex and Iatrobeads column chromatographies. The structures of these gangliosides were determined as Gmi(NeuGc), Gm3(NeuAc), GM3(NeuGc), GD3(NeuGc), GD3(NeuGc comes from NeuAc), GT3(NeuGc), and another GM3 cont...

journal_title：Journal of biochemistry

authors： Ando N,Yamakawa T

更新日期：1982-03-01 00:00:00

abstract：:To identify functionally essential sequences and residues of CTLA-2alpha, in vitro mutagenesis was carried out. The coefficient of inhibition (K(i)) was determined towards rabbit cathepsin L using Z-Phe-Arg-MCA as the substrate. Recombinant CTLA-2alpha inhibited the enzyme potently (K(i) = 15 nM). A truncated mutant, ...

journal_title：Journal of biochemistry

authors： Deshapriya RM,Yuhashi S,Usui M,Kageyama T,Yamamoto Y

更新日期：2010-03-01 00:00:00

abstract：:The functions of the helix E (W97-F105), an amphiphilic lumenal 310 helix of the major antenna of photosystem II (LHCII), are still unidentified. To elucidate the roles of individual amino acid residue of the helix E, alanine scanning mutagenesis has been performed to mutate every residue of this domain to alanine. Th...

journal_title：Journal of biochemistry

authors： Liu C,Rao Y,Zhang L,Yang C

更新日期：2014-10-01 00:00:00

abstract：:Streptomyces caespitosus neutral protease (ScNP) is one of the smallest metalloproteinase with a molecular mass of 14 kDa. Effects of solvent composition on ScNP activity were examined using a peptide substrate. The k(cat)/K(m) values of ScNP exhibited bell-shaped pH-dependence with the optimal pH of 6.4-7.0 and the p...

journal_title：Journal of biochemistry

authors： Inouye K,Shimada T,Yasukawa K

更新日期：2007-09-01 00:00:00

abstract：:We studied enzymatic activities in sea urchin egg extracts that phosphorylate myosin regulatory light chain (MRLC) from chicken gizzard smooth muscle. The activity in the presence of EGTA showed cell cycle-dependent changes similar to that of histone H1 kinase, namely, it peaked shortly before cleavage, while that in ...

journal_title：Journal of biochemistry

authors： Mishima M,Mabuchi I

更新日期：1996-05-01 00:00:00

abstract：:Fluorescence changes of 1-anilino-8-naphthalenesulfonic acid and 3,3'-dipropyl-2,2'-thiadicarbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increase...

journal_title：Journal of biochemistry

authors： Ueno T,Sekine T

更新日期：1978-10-01 00:00:00

abstract：:The terminal bases of the viral ribonucleic acid from the Caulobacter ribonucleic acid phage phiCp2 were examined. The viral ribonucleic acid contained guanosine triphosphate (pppG) at the 5'-terminus and adenosine (AOH) at the 3'-terminus. ...

journal_title：Journal of biochemistry

authors： Fujiki K,Fukuda A,Okada Y

更新日期：1978-07-01 00:00:00

abstract：:A p-nitrophenyl-alpha-D-glucopyranoside-hydrolyzing alpha-glucosidase from an obligate thermophile, Bacillus thermoglucosidius KP 1006, gave a triphasic relationship at pH 6.8 in the van't Hoff plot of Km, in the Arrhenius plot of the first order rate constant of inactivation with 0.1% sodium dodecyl sulfate, and in t...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nakamura N,Kishigami T,Abe S

更新日期：1980-03-01 00:00:00

abstract：:Bacterial flagellar motors exploit the electrochemical potential gradient of a coupling ion as energy source and are composed of stator and rotor proteins. Vibrio alginolyticus has a Na(+)-driven motor and its stator is composed of PomA and PomB. Recently, we isolated increased motility strains (sp1-sp4) from the PomA...

journal_title：Journal of biochemistry

authors： Terashima H,Terauchi T,Ihara K,Nishioka N,Kojima S,Homma M

更新日期：2013-08-01 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

journal_title：Journal of biochemistry

authors： Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

更新日期：2002-02-01 00:00:00

abstract：:Aldehyde reductases from several mammalian and avian tissues transferred the pro-4R hydrogen of NADPH to the substrate, whereas the stereospecificity of carbonyl reductases was not uniform being correlated with the ability to catalyze the oxidoreduction of hydroxysteroids. ...

journal_title：Journal of biochemistry

authors： Nakayama T,Hara A,Kariya K,Sawada H

更新日期：1985-10-01 00:00:00

abstract：:The mitogen-activated protein (MAP) kinase cascades play essential roles in a variety of cell processes by influencing transcriptional or translational regulation. ERKs play a central role in survival and mitogenic signaling, while JNKs and p38 MAP kinases are preferentially activated by environmental stresses and are...

journal_title：Journal of biochemistry

authors： Matsukawa J,Matsuzawa A,Takeda K,Ichijo H

更新日期：2004-09-01 00:00:00

abstract：:A rapid micro-assay method for gelatinolytic activitiy has been developed using 3H-labeled heat-denatured polymeric collagen (gelatin) as a substrate to investigate enzymes involved in the post-collagenase catabolism of collagen. The method is based on the incubation of gelatin with enzyme followed by determination of...

journal_title：Journal of biochemistry

authors： Sunada H,Nagai Y

更新日期：1980-06-01 00:00:00

abstract：:Soybean Bowman-Birk inhibitor, a double-headed inhibitor of trypsin and alpha-chymotrypsin, was treated with cyanogen bromide and then pepsin to yield two inhibitory active fragments. Structural investigation showed that one of the fragments was derived from the trypsin inhibitory domain and the other from the chymotr...

journal_title：Journal of biochemistry

authors： Odani S,Ikenaka T

更新日期：1978-03-01 00:00:00

abstract：:By monitoring the activation of protein C and the regulation of factor Xa-catalyzed thrombin formation by the activated protein C (APC) on the surface of human umbilical vein endothelial cells (HUVEC), we found that functional protein C was synthesized in cultured HUVEC and expressed thereon in the presence of vitamin...

journal_title：Journal of biochemistry

authors： Tanabe S,Sugo T,Matsuda M

更新日期：1991-06-01 00:00:00

abstract：:An inorganic pyrophosphatase [EC 3.6.1.1] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum acti...

journal_title：Journal of biochemistry

authors： Tominaga N,Mori T

更新日期：1977-02-01 00:00:00

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:The protein structure, kinin-releasing activity, and tissue distribution of four major proteinases of mouse submandibular gland (mK22, mK9, proteinase F, proteinase P) were studied. When compared with the deduced amino acid sequence of each member of the tissue (glandular) kallikrein gene family, the amino acid sequen...

journal_title：Journal of biochemistry

authors： Hosoi K,Tsunasawa S,Kurihara K,Aoyama H,Ueha T,Murai T,Sakiyama F

更新日期：1994-01-01 00:00:00

abstract：:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. Th...

journal_title：Journal of biochemistry

authors： Uchikoba T,Yonezawa H,Kaneda M

更新日期：1995-05-01 00:00:00

abstract：:Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported...

journal_title：Journal of biochemistry

authors： Kashima N,Matsui H,Yamasaki S,Kondo N,Hamuro J

更新日期：1987-10-01 00:00:00

abstract：:After the intraperitoneal administration of radioactive cholic acid to rats, the binding of the cholic acid to protein was immunologically investigated. The serum and liver cytosol were separately incubated with antiligandin or antialbumin immunoglobulin and then subjected to sucrose density gradient centrifugation. T...

journal_title：Journal of biochemistry

authors： Fukuda H,Iritani N

更新日期：1981-12-01 00:00:00

abstract：:1. Porcine cardiac native tropomyosin was phosphorylated by bovine cardiac 3':5'-cyclic AMP-dependent protein kinase. Most of the phosphate incorporation was observed in troponin I, the maximum of which was 0.7 mol of Pi per mol of troponin I. 2. In the presence of phosphorylated native tropomyosin, actomyosin ATPase ...

journal_title：Journal of biochemistry

authors： Yamamoto K,Ohtsuki I

更新日期：1982-05-01 00:00:00

abstract：:Heat-stable malate dehydrogenase isolated from Thermus flavus AT62 was completely inactivated by treatment with diethylpyrocarbonate. The inactivation was accompanied by the loss of 1.2 histidine residues per subunit of the enzyme. The enzyme was protected from inactivation by NADH. The enzyme was also inactivated by ...

journal_title：Journal of biochemistry

authors： Iijima S,Oh MJ,Saiki T,Beppu T

更新日期：1986-06-01 00:00:00