Up-regulation of telomerase in primary cultured rat hepatocytes.

abstract：:We have cloned a chitinase cDNA (MpChi-1) from plantain (Musa paradisiacal L) using rapid amplification of cDNA ends (RACE) according to a sequence fragment which we had cloned using the suppression subtractive hybridization (SSH) technique. The MpChi-1 encodes a protein of 326 amino acids and belongs to acidic chitin...

journal_title：Journal of biochemistry

authors： Fan J,Wang H,Feng D,Liu B,Liu H,Wang J

更新日期：2007-11-01 00:00:00

abstract：:Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1....

journal_title：Journal of biochemistry

authors： Komiya H,Takemura S

更新日期：1979-10-01 00:00:00

abstract：:Amino acid compositions of 356 proteins are expressed as points in an 18 dimensional space of 18 axes representing the contents of amino acids. The proteins are classified into four groups of intra- and extracellular enzymes and nonenzymes according to analysis of the distribution of the points. The groups have a sign...

journal_title：Journal of biochemistry

authors： Nishikawa K,Ooi T

更新日期：1982-05-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:The circadian change of gluconeogenesis in the liver in vivo in fed rats was studied. Gluconeogenesis in the liver in vivo was determined by measuring the rate of synthesis of 14C-labeled blood glucose derived from a 14C-labeled substrate, such as lactate, pyruvate or an amino acid injected into nephrectomized rats. G...

journal_title：Journal of biochemistry

authors： Kida K,Nishio T,Yokozawa T,Nagai K,Matsuda H,Nakagawa H

更新日期：1980-10-01 00:00:00

abstract：:Steady-state kinetic analyses suggest that Pseudomonas ochraceae 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde: NADP+ oxidoreductase [EC 1.2.1.45]) functions through an ordered BiBi mechanism. The enzyme binds one NADP+ molecule per subunit with a Kd of 4...

journal_title：Journal of biochemistry

authors： Maruyama K

更新日期：1988-04-01 00:00:00

abstract：:An inhibitor of neutral subtilopeptidase [EC 3.4.24.4] was purified from porcine serum by salting out with (NH4)2SO4, chromatography on anion exchange sephadex, gel filtration with Sepharose 6B, and isoelectric focusing. The preparation was homogeneous by electrophoretic and ultracentrifugal criteria, and was shown to...

journal_title：Journal of biochemistry

authors： Tsuru D,Tomimatsu M,Fujiwara K,Kawahara K

更新日期：1975-06-01 00:00:00

abstract：:We isolated three genomic clones of rat P-450(11 beta) genes (CYP11B). Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively. The third one was a novel gene resembling both CYP11B1 and CYP11B2, and was named CYP11B3 gene (CYP11B3). CYP11B2 and CYP11B3 are ...

journal_title：Journal of biochemistry

authors： Nomura M,Morohashi K,Kirita S,Nonaka Y,Okamoto M,Nawata H,Omura T

更新日期：1993-02-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00

abstract：:The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...

journal_title：Journal of biochemistry

authors： Murata D,Arai K,Iijima M,Sesaki H

更新日期：2020-03-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:Amino acid sequence analysis was done on a human lambda Bence Jones protein NIG-64 with the major objective of determining the sequence of the variable region. Nineteen tryptic peptides covering 216 residues were isolated from the completely reduced and aminoethylated protein, and 17 of these were completely sequenced...

journal_title：Journal of biochemistry

authors： Kametani F,Takayasu T,Suzuki S,Shinoda T,Okuyama T,Shimizu A

更新日期：1983-02-01 00:00:00

abstract：:We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 m...

journal_title：Journal of biochemistry

authors： Nagino K,Nomura O,Takii Y,Myomoto A,Ichikawa M,Nakamura F,Higasa M,Akiyama H,Nobumasa H,Shiojima S,Tsujimoto G

更新日期：2006-04-01 00:00:00

abstract：:beta-D-Galactosidase has been purified to apparent homogeneity from rabbit spleen. The purification steps involved ammonium sulphate precipitation, DEAE-cellulose, concanavalin A-Sepharose, Sephadex G-200, and Sepharose 4B-(epsilon-aminocaproyl)-2-deoxy-beta-D-glucosylamine affinity chromatographies. In the DEAE-cellu...

journal_title：Journal of biochemistry

authors： Rodríguez-Berrocal FJ,Páez de la Cadena M,Cabezas JA,Pérez-González N

更新日期：1988-07-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:Inhibitory PAS domain protein (IPAS) is a dual function protein acting as a transcriptional repressor and as a pro-apoptotic protein. Simultaneous dual-color single-molecule imaging of EGFP-IPAS coexpressed with Mit-TagRFP-T in living HeLa cells revealed that fraction of EGFP-IPAS was arrested in the nucleus and on mi...

journal_title：Journal of biochemistry

authors： Kasai S,Kajimoto S,Ito Y,Saito T,Yasumoto KI,Tokunaga M,Sakata-Sogawa K,Fukumura H,Sogawa K

更新日期：2017-03-01 00:00:00

abstract：:When meso-tetrakis(3-N-methylpyridiniumyl)porphyrin (m-TMPyP) formed a complex with poly[d(A-T)(2)], an intense bisignate excitonic CD in the Soret absorption region was observed. The excitonic CD of the m-TMPyP-poly[d(A-T)(2)] complex is unique in that no other combination of the related porphyrin, namely, meso-tetra...

journal_title：Journal of biochemistry

authors： Lee YA,Lee S,Lee HM,Lee CS,Kim SK

更新日期：2003-03-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00

abstract：:Ubiquinone was biosynthesized when rat liver mitochondria were incubated with S-adenosyl-L-methionine, solanesyl diphosphate, and [U-14C]p-hydroxybenzoate. The intermediates of ubiquinone biosynthesis but not ubiquinone were accumulated in mitochondria incubated without S-adenosyl-L-methionine and the accumulated inte...

journal_title：Journal of biochemistry

authors： Kang D,Fujiwara T,Takeshige K

更新日期：1992-03-01 00:00:00

abstract：:Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

journal_title：Journal of biochemistry

authors： Mima J,Suzuki H,Takahashi M,Hayashi R

更新日期：2002-12-01 00:00:00

abstract：:Electron transport particles (ETP) prepared from beef heart mitochondria formed malondialdehyde by NADPH-dependent lipid peroixidation in the presence of ferric ions and ADP or ATP. The reaction was inhibited by MnCl2, EDTA, or radical scavengers, but was not inhibited by p-hydroxymercuribenzoate (PHMB) or respiratory...

journal_title：Journal of biochemistry

authors： Takeshige K,Minadami S

更新日期：1975-05-01 00:00:00

abstract：:An NAD(P)H-dependent C-nitrosoreductase was purified 90 fold from porcine heart cytosol fraction by ammonium sulfate fractionation, gel filtration with Sephadex G-100, ion-exchange chromatography on DEAE-Sephadex A-50, and affinity chromatography on 5'-AMP-Sepharose. The enzyme had no aldehyde reductase activity and s...

journal_title：Journal of biochemistry

authors： Horie S,Ogura Y

更新日期：1980-10-01 00:00:00

abstract：:Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...

journal_title：Journal of biochemistry

authors： Haraguchi T,Nagano H

更新日期：1983-03-01 00:00:00

abstract：:Acid ceramidase (ACDase) metabolizes ceramide to sphingosine, leading to sphingosine 1-phosphate production. Reportedly, ACDase has been upregulated in prostate cancer. However, its regulatory mechanism remains unclear. LNCaP (androgen-sensitive prostate cancer cell line) but not PC3 and DU-145, (androgen-unresponsive...

journal_title：Journal of biochemistry

authors： Mizutani N,Inoue M,Omori Y,Ito H,Tamiya-Koizumi K,Takagi A,Kojima T,Nakamura M,Iwaki S,Nakatochi M,Suzuki M,Nozawa Y,Murate T

更新日期：2015-10-01 00:00:00

abstract：:In regenerating rat liver, nuclear protein histone H2A was shown to be phosphorylated on its amino-terminal serine residue [Sung et al. (1971) J. Biol. Chem. 246, 1358-1364], but the protein kinase which phosphorylates this residue has not been identified. To evaluate the possibility that protein kinase C can phosphor...

journal_title：Journal of biochemistry

authors： Takeuchi F,Hashimoto E,Yamamura H

更新日期：1992-06-01 00:00:00

abstract：:The location and state of an essential histidyl residue in a milk-clotting acid proteases, Mucor rennin, were investigated by NMR spectroscopy. Assignment of the C2H resonance peak of the essential histidyl residue was possible by comparison of the NMR spectrum of the native enzyme with that of the photo-oxidized enzy...

journal_title：Journal of biochemistry

authors： Etoh Y,Shoun H,Ogino T,Fujiwara S,Arima K,Beppu T

更新日期：1982-06-01 00:00:00

abstract：:We have isolated cDNAs and completed for the first time the primary structure for a novel collagenous chain that was partially characterized earlier and named alpha 1(Y) chain [Yoshioka, H. et al. (1992) Genomics 13, 884-886]. The size of the coding region was unexpectedly small compared with the length of the mRNA (>...

journal_title：Journal of biochemistry

authors： Inoguchi K,Yoshioka H,Khaleduzzaman M,Ninomiya Y

更新日期：1995-01-01 00:00:00

abstract：:The binding of nuclear envelope precursor vesicles and chromatin was characterized by using an in vitro system constituted from a Xenopus egg extract and demembranated Xenopus sperm chromatin. The results of binding studies in the presence of salts, urea, and a chelator showed that the binding involves an ionic intera...

journal_title：Journal of biochemistry

authors： Imai N,Sasagawa S,Yamamoto A,Kikuchi F,Sekiya K,Ichimura T,Omata S,Horigome T

更新日期：1997-11-01 00:00:00

abstract：:Human erytholeukemia K562 cells are induced to differentiate along the erythroid lineage by a variety of chemical compounds, including hemin, sodium butyrate and 1-β-d-arabinofuranosylcytosine. We have investigated the induction of erythroid differentiation of K562 cells by glutamine depletion. When K562 cells were cu...

journal_title：Journal of biochemistry

authors： Canh Hiep N,Kinohira S,Furuyama K,Taketani S

更新日期：2012-12-01 00:00:00