Abstract:
:Amino acid sequence analysis was done on a human lambda Bence Jones protein NIG-64 with the major objective of determining the sequence of the variable region. Nineteen tryptic peptides covering 216 residues were isolated from the completely reduced and aminoethylated protein, and 17 of these were completely sequenced. These comprised the entire variable region and 11 from the constant region. For the remaining peptides covering the rest of the constant region, only partial sequences or the amino acid compositions were determined. All the tryptic peptides could be arranged in order on the basis of the above results and homology with other human lambda light chains of the same isotype. The sequence of the variable region of the protein is highly homologous with that of protein New of subgroup V lambda I as compared with other proteins of the same subgroup, suggesting that subgroup V lambda I may be further divided into subsubgroups, namely subsubgroups V lambda I-1 and V lambda I-2.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Kametani F,Takayasu T,Suzuki S,Shinoda T,Okuyama T,Shimizu Adoi
10.1093/oxfordjournals.jbchem.a134196subject
Has Abstractpub_date
1983-02-01 00:00:00pages
421-9issue
2eissn
0021-924Xissn
1756-2651journal_volume
93pub_type
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