Abstract:
:Amino acid compositions of 356 proteins are expressed as points in an 18 dimensional space of 18 axes representing the contents of amino acids. The proteins are classified into four groups of intra- and extracellular enzymes and nonenzymes according to analysis of the distribution of the points. The groups have a significant correlation to four folding types of secondary structures, and extra- and intracellular proteins to those with and without disulfide bond(s), respectively. The location and function of a protein seem to determine its amino acid composition and folding type.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Nishikawa K,Ooi Tdoi
10.1093/oxfordjournals.jbchem.a133877subject
Has Abstractpub_date
1982-05-01 00:00:00pages
1821-4issue
5eissn
0021-924Xissn
1756-2651journal_volume
91pub_type
杂志文章abstract::Affinity chromatography based on the reaction between SH groups in protein and +HgC6H4CO groups in the p-mercuribenzoylaminoethyl derivative of Sepharose 4B was examined with a crude preparation of calf thymus cysteine-containing histone. Adsorption of the histone onto the column by specific coupling was found to be ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130737
更新日期:1975-02-01 00:00:00
abstract::The phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme catalyzes reversibly the intra-molecular phosphoryl interconverting reaction of mannose-6-phosphate and mannose-1-phosphate or glucose-6-phosphate and glucose-1-phosphate. Glucose-6-phosphate and glucose-1-phosphate are known to be utilized for energy metaboli...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvi115
更新日期:2005-08-01 00:00:00
abstract::We previously reported a new laminin variant containing laminin gamma 2 (or B2t) chain, ladsin, which exerted prominent cell-scattering, cell-adhesion, and cell-migration activities. In the present study, this laminin was further characterized, and gene expression of its three subunits in various human tissues and can...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021541
更新日期:1996-12-01 00:00:00
abstract::Papain [EC 3.4.22.2] polymerizes readily upon treatment with tetranitromethane (TNM) by forming intermolecular covalent linkages through its tyrosine residues (Tsukamoto, S. & Ohno, M. (1974) J. Biochem. 75, 1377-1380). Polymerization occurred optimally at pH 9.0 with S-sulfenylsulfonate papain. Circular dichroic spec...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132289
更新日期:1978-12-01 00:00:00
abstract::Comparative studies of the unfolding equilibria of two homologous proteins, bovine alpha-lactalbumin and hen lysozyme, induced by treatment with guanidine hydrochloride have been made by analysis of the peptide and the aromatic circular dichroism spectra. The effect of the specific binding of Ca2+ ion by the former pr...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135582
更新日期:1986-04-01 00:00:00
abstract::Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/jb/mvq052
更新日期:2010-07-01 00:00:00
abstract::As a new strategy for drug discovery and development, I focus on drug re-profiling as a way to identify new treatments for diseases. In this strategy, the actions of existing medicines, whose safety and pharmacokinetic effects in humans have already been confirmed clinically and approved for use, are examined comprehe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvr032
更新日期:2011-05-01 00:00:00
abstract::Spectrophotometric studies revealed the irreversible photodissociation of reduced cytochrome oxidase-nitric oxide (NO) at 5 K. The dissociated NO recombined as the sample temperature was raised, and the half-recombination temperature was 65 K. The photodissociation at 5K was also confirmed by a change in the EPR spect...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133138
更新日期:1980-12-01 00:00:00
abstract::Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124099
更新日期:1993-06-01 00:00:00
abstract::Basigin belongs to the immunoglobulin superfamily and may be related to the primordial form of the superfamily. Human basigin cDNA was isolated and sequenced, and the predicted protein structure was compared with that of mouse basigin and two related molecules, embigin and the chicken blood-brain barrier antigen HT7. ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123657
更新日期:1991-11-01 00:00:00
abstract::I attempted to isolate synaptic vesicles by gel filtration. The rat brain synaptic vesicles in a synaptosomal lysate were collected by ammonium sulfate salting-out and fractionated on a Sephacryl S-500 with a mean exclusion size of 200 nm. Peak I at the void volume contained large vesicular membranes and coated vesicl...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvg057
更新日期:2003-04-01 00:00:00
abstract::A crude preparation of membrane-bound phospholipase A (detergent-resistant) in Escherichia coli K-12 cells was found to be quite stable or even apparently activated on incubation at 100 degrees C, but became strikingly thermolabile when it was highly purified and Triton X-100 was removed from the purified enzyme prepa...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132607
更新日期:1979-10-01 00:00:00
abstract::Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvaa082
更新日期:2020-12-26 00:00:00
abstract::The presence of two genomic DNA regions encoding malate synthase (MS) was shown by Southern blot analysis of the genomic DNA from an n-alkane-assimilating yeast, Candida tropicalis, using a partial MS cDNA probe, in accordance with the fact that two types of partial MS cDNAs have previously been isolated. This was als...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123688
更新日期:1991-12-01 00:00:00
abstract::GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/93.3.895
更新日期:1983-03-01 00:00:00
abstract::The three-dimensional structure of rat-liver acyl-CoA oxidase-II (ACO-II) in a complex with a C12-fatty acid was solved by the molecular replacement method based on the uncomplexed ACO-II structure. The crystalline form of the complex was obtained by cocrystallization of ACO-II with dodecanoyl-CoA. The crystalline com...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj088
更新日期:2006-04-01 00:00:00
abstract::Proteolytic trimming of isoprenylated proteins, including Ras, at the C-terminal CAAX motifs is a key event in their activation. However, the protease responsible for the proteolysis has not been well characterized yet. In this study, we established a novel assay method for the enzyme using a fluorescent substrate, da...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021767
更新日期:1997-08-01 00:00:00
abstract::Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021583
更新日期:1997-02-01 00:00:00
abstract::Pure bovine rhodopsin pellets were prepared by removal of cholic acid from rhodopsin-cholic acid complex, and X-ray diffraction patterns from the internal structure of rhodopsin were obtained for the first time using wet and dry pellet samples. The peaks at around 10 and 4.3 A spacings observed for both samples can be...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021254
更新日期:1996-03-01 00:00:00
abstract::A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135549
更新日期:1986-03-01 00:00:00
abstract::In eukaryotes, ribonuclease H1 (RNase H1) is involved in the processing and removal of RNA/DNA hybrids in both nuclear and mitochondrial DNA. The enzyme comprises a C-terminal catalytic domain and an N-terminal hybrid-binding domain (HBD), separated by a linker of variable length, 115 amino acids in Drosophila melanog...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvaa067
更新日期:2020-11-01 00:00:00
abstract::C-Proteins in developing, denervated, and dystrophic chicken skeletal muscles were examined by means of two-dimensional (2D) gel electrophoresis in combination with immunoblotting. In this analysis, the electrophoresis system which was devised by Hirabayashi (Anal. Biochem. 117, 443-451, 1981) provided excellent resol...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123069
更新日期:1990-03-01 00:00:00
abstract::Cholesterol: oxygen oxidoreductase [EC 1.1.3.6] from Brevibacterium sterolicum (ATCC 21387) was found to catalyze the oxidation of steroids such as sterols, steroid hormones, and bile acids having a free C-3beta hydroxyl group. However, the enzyme was inactive towards estradiol and estriol and had a weak activity towa...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132472
更新日期:1979-06-01 00:00:00
abstract::The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132810
更新日期:1980-03-01 00:00:00
abstract::Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) mainly catalyzes oxygenation when L-phenylalanine is used as the substrate, but oxidation when L-methionine is used as the substrate. Using [C(alpha)-H]-DL-methionine and [C(alpha)-D]-DL-methionine as substrate, the reductive half reaction of FAD co...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj049
更新日期:2006-03-01 00:00:00
abstract::Light harvesting (LH) bacteriochlorophyll (Bchl) protein complexes were isolated from chromatophores of Chromatium vinosum, by the combination of detergent solubilization, sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and hydroxyapatite chromatography. Addition of the absorption spectra of thes...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133093
更新日期:1980-11-01 00:00:00
abstract::It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131018
更新日期:1975-12-01 00:00:00
abstract::To investigate the role of the C-terminal region of barley beta-amylase, plasmid delta 54 was constructed with an expression vector (pBETA92) of barley beta-amylase by site-directed mutagenesis. Escherichia coli JM109 harboring plasmid delta 54 was expected to express delta 54 beta-amylase in which 54 amino acid resid...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124722
更新日期:1995-01-01 00:00:00
abstract::The isotypes of sarcoplasmic Ca2+ binding protein (SCP) were purified from shrimp tail muscle. SCP exists in a dimeric form. One sample of shrimp contained only alpha A chain, whereas another contained alpha B and beta chains, and a heterodimer of alpha B beta which was not analyzed precisely. The amino acid sequences...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134773
更新日期:1984-06-01 00:00:00
abstract::Recently, we revealed that the cloverleaf structure of some eukaryotic tRNAs is not always stable in vitro, and the denatured structures of these tRNAs are sometimes detected in bacterial RNase P reactions. We have designated the unusual internal cleavage reaction of these tRNAs as hyperprocessing. We have developed t...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003173
更新日期:2002-06-01 00:00:00