Photodissociation of cytochrome oxidase-nitric oxide at low temperatures.

abstract：:Previously, we isolated t-cinnamoyl-D-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. The enzyme which catalyzes the formation of t-cinnamoyl-D-glucose has been purified 539-fold from sweet potato root (Ipomoea batatas Lam.) and characterized. It required UDP-glucose as a gl...

journal_title：Journal of biochemistry

authors： Shimizu T,Kojima M

更新日期：1984-01-01 00:00:00

abstract：:The mitochondrial regulatory region (mrr) located between the tRNAPhe and tRNAPro genes of mitochondrial DNA (mtDNA) is essential for regulation of replication and transcription of the mitochondrial genome. Polyadenylated short RNAs complementary to the L-strand of the mrr in human cells and similar RNAs (polyadenylat...

journal_title：Journal of biochemistry

authors： Nakamichi N,Rhoads DD,Hayashi JI,Kagawa Y,Matsumura T

更新日期：1998-03-01 00:00:00

abstract：:Glycogen phosphorylase (GP) is biologically active as a dimer of identical subunits. Each subunit has two distinct maltooligosaccharide binding sites: a storage site and a catalytic site. Our characterization of the properties of these sites suggested that GP activity consists of two activities: (i) binding to the gly...

journal_title：Journal of biochemistry

authors： Makino Y,Fujii Y,Taniguchi M

更新日期：2015-06-01 00:00:00

abstract：:The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In ...

journal_title：Journal of biochemistry

authors： Takiya S,Saito S,Yokoyama T,Matsumoto D,Aizawa T,Kamiya M,Demura M,Kawano K

更新日期：2009-07-01 00:00:00

abstract：:The SoxR protein of Escherichia coli responds to redox signals by activating the transcription of soxS, which encodes another transcription activator that directly stimulates oxidative stress genes. In contrast, Pseudomonas aeruginosa has an open reading frame (ORF) encoding a putative protein homologous to E. coli So...

journal_title：Journal of biochemistry

authors： Kobayashi K,Tagawa S

更新日期：2004-11-01 00:00:00

abstract：:By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

journal_title：Journal of biochemistry

authors： Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

更新日期：1982-06-01 00:00:00

abstract：:A p-nitrophenyl-alpha-D-glucopyranoside-hydrolyzing alpha-glucosidase from an obligate thermophile, Bacillus thermoglucosidius KP 1006, gave a triphasic relationship at pH 6.8 in the van't Hoff plot of Km, in the Arrhenius plot of the first order rate constant of inactivation with 0.1% sodium dodecyl sulfate, and in t...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nakamura N,Kishigami T,Abe S

更新日期：1980-03-01 00:00:00

abstract：:After a dose of 10 micrograms of 6-aminonicotinamide (6-AN) was administered to day-4- chick embryo in ovo, micromelia was obviously observed in the hind limbs of 7-day chick embryos. We examined the teratogenic mechanism of 6-AN by using the normal or micromelial hind limbs (buds) from day 5 to day 7, with special at...

journal_title：Journal of biochemistry

authors： Honda A,Iwama M,Umeda T,Mori Y

更新日期：1982-06-01 00:00:00

abstract：:Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

journal_title：Journal of biochemistry

authors： Soh KY,Loh JMS,Proft T

更新日期：2018-08-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:While many reports have shown that Ca2+ alone causes ryanodine binding to the heavy fraction of the sarcoplasmic reticulum (HFSR), our results demonstrate that caffeine or beta,gamma-methylene adenosine triphosphate (AMPOPCP) in addition to Ca2+ is necessary for ryanodine binding, although Ca2+ is indispensable for it...

journal_title：Journal of biochemistry

authors： Ogawa Y,Harafuji H

更新日期：1990-06-01 00:00:00

abstract：:The 565 amino-acid PRIB protein with a Zn(II)2Cys6 zinc-cluster DNA-binding motif is the expression product of the priB gene, which is most actively transcribed in an early stage of fruiting-body formation by the basidiomycete, Lentinus edodes. PRIB produced in Escherichia coli using the bacteriophage T7 expression sy...

journal_title：Journal of biochemistry

authors： Miyazaki Y,Tsunoka O,Shishido K

更新日期：1997-12-01 00:00:00

abstract：:We generated and characterized novel antibodies specific for a cleavage site of human caspase-8/FLICE and its substrate, FLICE-like inhibitory protein (FLIP). The synthetic peptides used as immunogens were CQGDNYQKGIPVETD (#791) and VSEGQLEDSSLLEVD (#1342), which corresponded to cleaved regions of N-terminal fragments...

journal_title：Journal of biochemistry

authors： Niikura Y,Nonaka T,Imajoh-Ohmi S

更新日期：2002-07-01 00:00:00

abstract：:The purpose of this study was to determine whether ATP-sensitive potassium channel (K(ATP) channel) activation generates oxygen free radicals in the rabbit heart. We assayed malondialdehyde (MDA) in rabbit heart slices in vitro as an indicator of oxygen free radical generation. The K(ATP) channel openers, pinacidil an...

journal_title：Journal of biochemistry

authors： Han J,Kim N,Park J,Seog DH,Joo H,Kim E

更新日期：2002-05-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-compl...

journal_title：Journal of biochemistry

authors： Baba S,Takahashi K,Noguchi S,Takaku H,Koyanagi Y,Yamamoto N,Kawai G

更新日期：2005-11-01 00:00:00

abstract：:To model gene transcription kinetics, empirical fitting with the Hill function or S-system is often used. In this study, we derived an analytical expression for gene transcription rates in a manner similar to that developed for enzyme kinetics to describe the kinetics of gene transcription mediated by dimeric transcri...

journal_title：Journal of biochemistry

authors： Yang HT,Hsu CP,Hwang MJ

更新日期：2007-08-01 00:00:00

abstract：:An H2O2-generating fraction was prepared from porcine thyroid homogenate by differential and Percoll-density gradient centrifugations. The fraction consisted of mainly fragmented plasma membranes as judged by marker enzyme analysis and electron microscopy. The fraction produced H2O2 by reaction with NADPH only in the ...

journal_title：Journal of biochemistry

authors： Nakamura Y,Ogihara S,Ohtaki S

更新日期：1987-11-01 00:00:00

abstract：:Recombinant human granulocyte-colony-stimulating factor (G-CSF) was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA. The recombinant human G-CSF was treated with alkaline borohydride and the oligosaccharide-alditols liberated were fractioned by gel filtration on a Bio-Gel P-4 column, follow...

journal_title：Journal of biochemistry

authors： Oheda M,Hase S,Ono M,Ikenaka T

更新日期：1988-03-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:A contractile protein closely resembling natural actomyosin (myosin B) of rabbit skeletal muscle was extracted from plasmodia of the slime mold, Physarum polycephalum, by protecting the SH-groups with beta-mercaptoethanol or dithiothreitol. Superprecipitation of the protein induced by Mg2+-ATP at low ionic strength wa...

journal_title：Journal of biochemistry

authors： Kato T,Tonomura Y

更新日期：1975-06-01 00:00:00

abstract：:A DNA polymerase beta (pol. beta) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-beta-D-glucoside, prunasin. The compound inhib...

journal_title：Journal of biochemistry

authors： Mizushina Y,Takahashi N,Ogawa A,Tsurugaya K,Koshino H,Takemura M,Yoshida S,Matsukage A,Sugawara F,Sakaguchi K

更新日期：1999-08-01 00:00:00

abstract：:Of the five HSV1 immediate-early proteins, infected-cell protein 22 (ICP22), the product of the Us1 gene, is a member whose function is less understood. In order to promote better understanding of the role of ICP22 in viral replication, mutation and fluorescence techniques were used to investigate the biochemical rela...

journal_title：Journal of biochemistry

authors： Cun W,Hong M,Liu LD,Dong CH,Luo J,Li QH

更新日期：2006-07-01 00:00:00

abstract：:Glucuronolactone reductase [EC 1.1.1.20] from rat kidney was purified over 300-fold by ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite columns, and preparative isoelectric focusing. The substrate specificity of the enzyme in the reduction reaction was broad, and hexuronic acid was ...

journal_title：Journal of biochemistry

authors： Hayashi S,Watanabe M,Kimura A

更新日期：1984-01-01 00:00:00

abstract：:Caldesmon, calmodulin-, and actin-binding protein of chicken gizzard did not affect the process of polymerization of actin induced by 0.1 M KCl. Caldesmon binds to F-actin, thus inhibiting the gelation action of actin binding protein (ABP; filamin). Low shear viscosity and flow birefringence measurements revealed that...

journal_title：Journal of biochemistry

authors： Maruyama K,Morimoto K,Kijima Y,Sobue K,Kakiuchi S

更新日期：1985-05-01 00:00:00

abstract：:The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...

journal_title：Journal of biochemistry

authors： Miyanishi T,Toyoshima C,Wakabayashi T,Matsuda G

更新日期：1988-03-01 00:00:00

abstract：:The transcription and replication of influenza RNA can be studied in vitro by the reconstitution of functional ribonucleoprotein (RNP) complex from viral core proteins including the RNA polymerase (complex of three P protein subunits) and nucleoprotein (NP), and model templates. Here, two different core protein prepar...

journal_title：Journal of biochemistry

authors： Seong BL,Kobayashi M,Nagata K,Brownlee GG,Ishihama A

更新日期：1992-04-01 00:00:00

abstract：:The receptor protein for the mitochondrial protein precursor synthesized in the cytosol was extensively purified from the mitochondrial membrane fraction by affinity column chromatography using a synthetic peptide containing the extrapeptide of ornithine aminotransferase as a ligand. The purified fraction contained tw...

journal_title：Journal of biochemistry

authors： Ono H,Tuboi S

更新日期：1990-06-01 00:00:00

abstract：:From the homogenate of rat submaxillary gland, two kinds of serine proteinases, named tentatively proteinases A and B, were isolated and their chemical properties and activities toward rat kininogens were examined, in comparison with those of submaxillary kallikrein. Proteinase A with Mr of 28,200 rapidly cleaved high...

journal_title：Journal of biochemistry

authors： Kato H,Nakanishi E,Enjyoji K,Hayashi I,Oh-ishi S,Iwanaga S

更新日期：1987-12-01 00:00:00