Transformation of leukotriene D4 catalyzed by lysosomal cathepsin H of rat liver.

abstract：:Two transferrin (Tf) glycan-isoforms were previously found in cerebrospinal fluid (CSF); one appears to be derived from serum (Tf-2) and the other from choroid plexus, a CSF-producing tissue (Tf-1). To analyse metabolic differences associated with the two isoforms, their ratio (Tf-2/Tf-1) was defined as the Tf index. ...

journal_title：Journal of biochemistry

authors： Yoshihara A,Fukatsu M,Hoshi K,Ito H,Nollet K,Yamaguchi Y,Ishii R,Tokuda T,Miyajima M,Arai H,Kato T,Furukawa K,Arai H,Kikuchi A,Takeda A,Ugawa Y,Hashimoto Y

更新日期：2016-08-01 00:00:00

abstract：:Three-dimensional images of the actin-tropomyosin-myosin subfragment-1 (S1) complex were reconstituted from both minimal- and high-dose electron micrographs by using a conventional reconstruction technique. Higher resolution (1/15 A-1) than those of the previous reconstructions was attained. A multi-domain structure s...

journal_title：Journal of biochemistry

authors： Toyoshima C,Wakabayashi T

更新日期：1985-01-01 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four ...

journal_title：Journal of biochemistry

authors： Hirose J,Sakurai T,Imamura K,Watanabe H,Iwamoto H,Hiromi K,Itoh H,Shin T,Murao S

更新日期：1994-05-01 00:00:00

abstract：:Activity-oligomeric assembly relationships using octaethylene glycol dodecyl ether (C12E8) solubilized pig gastric H/K-ATPase (unmodified H/K-ATPase) or H/K-ATPase modified with Fluorescein 5'-isothiocyanate (FITC-H/K-ATPase) were examined. The amount of oligomeric species in FITC-H/K-ATPase, which retained little H/K...

journal_title：Journal of biochemistry

authors： Abe K,Kaya S,Taniguchi K,Hayashi Y,Imagawa T,Kikumoto M,Oiwa K,Sakaguchi K

更新日期：2005-09-01 00:00:00

abstract：:Cytochrome c oxidase from Thiobacillus ferrooxidans was purified to homogeneity and some of its properties were studied. The oxidase was solubilized with n-octyl-beta-D-thioglucoside (OTG) under acidic conditions (pH 4.0) and purified by one step of ion-exchange chromatography with a CM-Toyopearl column. The absorptio...

journal_title：Journal of biochemistry

authors： Kai M,Yano T,Tamegai H,Fukumori Y,Yamanaka T

更新日期：1992-12-01 00:00:00

abstract：:The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or ...

journal_title：Journal of biochemistry

authors： Takigawa M,Watanabe R,Ishida H,Asada A,Suzuki F

更新日期：1979-01-01 00:00:00

abstract：:Heat-stable malate dehydrogenase isolated from Thermus flavus AT62 was completely inactivated by treatment with diethylpyrocarbonate. The inactivation was accompanied by the loss of 1.2 histidine residues per subunit of the enzyme. The enzyme was protected from inactivation by NADH. The enzyme was also inactivated by ...

journal_title：Journal of biochemistry

authors： Iijima S,Oh MJ,Saiki T,Beppu T

更新日期：1986-06-01 00:00:00

abstract：:1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z...

journal_title：Journal of biochemistry

authors： Muguruma M,Muguruma Y,Fukazawa T

更新日期：1980-07-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00

abstract：:The FLI-1 transcription factor is a member of the ETS gene family, most closely related to ERG. In this study, the FLI-1 protein products were characterized using a specific monoclonal antibody previously developed against bacterially expressed protein. In the human T-cell line Jurkat, both isoforms of FLI-1, p51 and ...

journal_title：Journal of biochemistry

authors： Zhang XK,Watson DK

更新日期：2005-03-01 00:00:00

abstract：:Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

journal_title：Journal of biochemistry

authors： Narutaki S,Dunn BM,Oda K

更新日期：1999-01-01 00:00:00

abstract：:Covalently closed-circular, superhelical DNAs, including viral DNAs, bacterial plasmid DNAs, and bacteriophage replicative-form DNA, were treated with a small amount of Haemophilus gallinarum DNA-relaxing enzyme to generate incompletely relaxed DNA molecules. Each sample consisted of a set of closed-circular DNA molec...

journal_title：Journal of biochemistry

authors： Shishido K

更新日期：1979-09-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00

abstract：:Acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was purified from rat liver. The final preparation was judged to be nearly homogeneous from the results of sedimentation analysis. Ultrogel AcA-34 column chromatography, and phosphocellulose column chromatography. The molecular weight of th...

journal_title：Journal of biochemistry

authors： Osumi T,Hashimoto T,Ui N

更新日期：1980-06-01 00:00:00

abstract：:Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...

journal_title：Journal of biochemistry

authors： Hamidi S,Sheng G

更新日期：2018-10-01 00:00:00

abstract：:By employing EPR spectrometry with the aid of a spin-trapping agent, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO), the generation of superoxide anion and hydroxyl radical was reevaluated during the respiratory burst of porcine and human neutrophils. Properly prepared resting neutrophils did not generate any spin-trapped ra...

journal_title：Journal of biochemistry

authors： Ueno I,Kohno M,Mitsuta K,Mizuta Y,Kanegasaki S

更新日期：1989-06-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:The relationship between DNA structure of replacement vectors and gene targeting efficiency was studied using positive-negative selection. The vectors contained pBR322 DNA, a bacterial neomycin-resistance gene (neo) for positive selection, a herpes simplex virus (HSV) thymidine kinase gene (tk) for negative selection,...

journal_title：Journal of biochemistry

authors： Horie K,Nishiguchi S,Maeda S,Shimada K

更新日期：1994-03-01 00:00:00

abstract：:A gene encoding an Na+/H+ antiporter was cloned from chromosomal DNA of the slightly halophilic marine bacterium Vibrio parahaemolyticus. The host was an Escherichia coli mutant that lacked both of the two major Na+/H+ antiporters, NhaA and NhaB. Untransformed mutant cells were unable to grow in the presence of 0.6 M ...

journal_title：Journal of biochemistry

authors： Kuroda T,Shimamoto T,Inaba K,Tsuda M,Tsuchiya T

更新日期：1994-11-01 00:00:00

abstract：:Two kinds of myosin phosphatases were purified from fresh chicken gizzard smooth muscle. Alkaline phosphatase (CGP-a), which requires Mg2+, was most active at pH 8.6 with 2 to 4 mM Mg2+, and was essentially the same as the phosphatase we reported previously (J. Biochem. 99, 1027-1036 (1986]. On the other hand, neutral...

journal_title：Journal of biochemistry

authors： Yoshida M,Yagi K

更新日期：1988-02-01 00:00:00

abstract：:We previously isolated a Ca2+-binding protein from a ciliate, Tetrahymena, and designated it as TCBP (Tetrahymena Ca2+-binding protein). The present paper reports that TCBP, which has two high affinity Ca2+-binding sites (Kd=4.6 X 10(-6) M), could activate porcine brain cyclic nucleotide phosphodiesterase at a concent...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nagao S,Abe K,Hirabayashi T,Watanabe Y

更新日期：1981-01-01 00:00:00

abstract：:Capacitative Ca(2+) entry is essential for refilling intracellular Ca(2+) stores and is thought to be regulated primarily by inositol 1, 4,5-trisphosphate (IP(3))-sensitive stores in nonexcitable cells. In nonexcitable A549 cells, the application of caffeine or ryanodine induces Ca(2+) release in the absence of extrac...

journal_title：Journal of biochemistry

authors： Xue HH,Zhao DM,Suda T,Uchida C,Oda T,Chida K,Ichiyama A,Nakamura H

更新日期：2000-08-01 00:00:00

abstract：:The oxygen exchange during ATP hydrolysis by glycerinated muscle fibers, myofibrils, and synthetic actomyosin filaments was studied from the distribution of the [18O]Pi species produced by the hydrolysis of [gamma-18O]ATP. The products were mixtures of two species, one with a low extent of oxygen exchange and the othe...

journal_title：Journal of biochemistry

authors： Yasui M,Ohe M,Kajita A,Arata T,Inoue A

更新日期：1989-04-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:The denaturation of recombinant horse L-chain apoferritin (rLF), which is composed of 24 L-chain subunits, in acidic solution was studied. Using two rLF mutants, lacking four (Fer4) or eight (Fer8) N-terminal amino acid residues, the effect of N-terminal residues on the protein's stability was investigated. Of the two...

journal_title：Journal of biochemistry

authors： Yoshizawa K,Mishima Y,Park SY,Heddle JG,Tame JR,Iwahori K,Kobayashi M,Yamashita I

更新日期：2007-12-01 00:00:00

abstract：:When crude neurofilaments were dissolved in a solution containing 8 M urea and 1% beta-mercaptoethanol (beta-ME), the component proteins of the neurofilaments and other contaminating filaments were solubilized into monomeric forms. However, when reassembled filaments were solubilized again by the addition of urea to 8...

journal_title：Journal of biochemistry

authors： Tokutake S,Tanaka K

更新日期：1989-01-01 00:00:00

abstract：:The effect of extracellular type-II phospholipase A2 (PLA2) on prostaglandin (PG) synthesis has been studied using rat liver-derived BRL-3A cells. The addition of type-II PLA2 to the medium of BRL-3A cells resulted in a marked decrease in the enzymatic activity in the medium. An immunochemical study involving an anti-...

journal_title：Journal of biochemistry

authors： Suga H,Murakami M,Kudo I,Inoue K

更新日期：1995-11-01 00:00:00

abstract：:We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. Th...

journal_title：Journal of biochemistry

authors： Ema M,Matsushita N,Sogawa K,Ariyama T,Inazawa J,Nemoto T,Ota M,Oshimura M,Fujii-Kuriyama Y

更新日期：1994-10-01 00:00:00