The significance of membrane lipids in exocytosis: control of liposome-evoked amylase release from secretory granules isolated from the rat parotid gland.


:We investigated the significance of the plasma membrane lipid composition in exocytosis in an in vitro interaction system using an intact secretory granular fraction (SG) isolated from the rat parotid gland. When various liposomes (as a model of plasma membranes) were added to this assay system, rapid and transient amylase release from the SG was evoked and increased by Ca2+ in a concentration-dependent manner. The extent depended upon not only the amount of liposomes but also their lipid composition. The addition of 1,2-diacylglycerol and phosphatidic acid to egg yolk phosphatidylcholine-liposomes remarkably facilitated the release. On the other hand, that of various free fatty acids had different effects depending upon their molecular species. Furthermore, a fluorescence de-quenching study demonstrated that membrane fusion actually occurred in this interaction system, and appeared to correlate with the amylase release. These results suggest that the transient alteration of the membrane lipid composition upon cell activation is a modulator of the exocytotic membrane interaction.


J Biochem


Journal of biochemistry


Mizuno-Kamiya M,Inokuchi H,Kameyama Y,Yashiro K,Shin SO,Fujita A




Has Abstract


1995-10-01 00:00:00












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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Cheang OO K

    更新日期:1975-03-01 00:00:00

  • Elucidation of the phenotypic change on the surface of Had-1 cell, a mutant cell line of mouse FM3A carcinoma cells selected by resistance to Newcastle disease virus infection.

    abstract::Had-1, which was isolated from mouse FM3A carcinoma cells, was a non-permissive mutant cell line to Newcastle disease virus infection. Comparative study of the asparagine-linked sugar chains of the surface glycoproteins of the mutant and its parental cells revealed that galactosylation of the complex-type sugar chains...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hara T,Endo T,Furukawa K,Kawakita M,Kobata A

    更新日期:1989-08-01 00:00:00

  • Site-directed mutagenesis of a thermostable alpha-amylase from Bacillus stearothermophilus: putative role of three conserved residues.

    abstract::The relationship between structure, activity, and stability of the thermostable Bacillus stearothermophilus alpha-amylase was studied by site-directed mutagenesis of the three most conserved residues. Mutation of His-238 to Asp involved in Ca2+ and substrate binding reduced the specific activity and thermal stability,...

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    pub_type: 杂志文章


    authors: Vihinen M,Ollikka P,Niskanen J,Meyer P,Suominen I,Karp M,Holm L,Knowles J,Mäntsälä P

    更新日期:1990-02-01 00:00:00

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    abstract::Guanidine hydrochloride (GdnHCl)-induced unfolding of bovine spleen galectin-1 (Gal-1) exhibits three-state mechanism involving exclusive, structured tertiary monomer in 0.5 M GdnHCl. Gal-1 has one tryptophan residue (Trp 68) per subunit. Phosphorescence spectra of both Gal-1 dimer and intermediate monomer at 77 K sho...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mandal P,Molla AR,Mandal DK

    更新日期:2013-12-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhang R,Yatsuki H,Kusakabe T,Iwabe N,Miyata T,Imai T,Yoshida M,Hori K

    更新日期:1995-03-01 00:00:00

  • Immunochemical studies on non-precipitating guinea pig antibody produced by administration of an excessive dose of Bacillus subtilis alpha-amylase.

    abstract::Administration of an excessive dose of Bacillus subtilis alpha-amylase [EC, alpha-1,4-glucan 4-glucanohydrolase] (BalphaA) induced the production of non-precipitating (non-ppt) IgG2 antibody in guinea pigs, whereas immunization with a normal dose produced precipitating (ppt) IgG1 and IgG2 antibodies. The non-p...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mori N,Nakamura T,Koyama J

    更新日期:1977-07-01 00:00:00

  • An intermediate of ubiquinone biosynthesis exists in the microsomal fraction of HepG2 cells.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


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    更新日期:1990-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Ohta T,Kawamura M,Hasegawa T,Ishikura H,Nagano K

    更新日期:1982-01-01 00:00:00

  • Genetic regulation of GM2 expression in liver of mouse.

    abstract::GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

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    pub_type: 杂志文章


    authors: Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

    更新日期:1983-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Honda A,Iwama M,Umeda T,Mori Y

    更新日期:1982-06-01 00:00:00

  • Opening of mitochondrial ATP-sensitive potassium channels evokes oxygen radical generation in rabbit heart slices.

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    pub_type: 杂志文章


    authors: Han J,Kim N,Park J,Seog DH,Joo H,Kim E

    更新日期:2002-05-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kigawa T,Yokoyama S

    更新日期:1991-08-01 00:00:00

  • Purification and characterization of a new GTP-binding protein of Mr 24,000 in bovine brain membranes.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohoka Y,Imai S,Kozasa T,Maehama T,Takahashi K,Kaziro Y,Ui M,Katada T

    更新日期:1991-03-01 00:00:00

  • gamma-Carboxyglutamic acid (Gla)-domainless blood coagulation factor IXa species: preparation and properties.

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    pub_type: 杂志文章


    authors: Morita T,Fukudome K,Miyata T,Iwanaga S

    更新日期:1991-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Ishihama A,Ikeuchi T,Matsumoto A,Yamamoto S

    更新日期:1976-05-01 00:00:00

  • Effect of N-terminal residues on the structural stability of recombinant horse L-chain apoferritin in an acidic environment.

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    pub_type: 杂志文章


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    更新日期:2007-12-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Jung G,Ueno H,Hayashi R

    更新日期:1998-08-01 00:00:00

  • DNA synthesis by fragment assembly using extra-cellular DNA delivered by artificial controlled horizontal transfer.

    abstract::DNA synthesis in the Bacillus subtilis cells has become possible using extra-cellular DNA. Generally, purified DNAs in a test tube have been required to introduce into the host cells for molecular cloning technology in the laboratory. We have developed a cell lysis technique for natural transformation using stable ext...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kaneko S,Fukushima H,Nakahama M,Asano S,Miyazaki Y,Aizawa Y,Itaya M

    更新日期:2018-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Tsunasawa S,Izu Y,Miyagi M,Kato I

    更新日期:1997-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Kusano M,Yasukawa K,Inouye K

    更新日期:2009-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

    更新日期:1983-12-01 00:00:00

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    abstract::Actin-microtubule crosstalk is implicated in the formation of cellular protrusions, but the mechanism remains unclear. In this study, we examined the regulation of cell protrusion involving a ubiquitously expressed microtubule-associated protein (MAP) 4, and its superfamily proteins, neuronal MAP2 and tau. Fluorescenc...

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    pub_type: 杂志文章


    authors: Doki C,Nishida K,Saito S,Shiga M,Ogara H,Kuramoto A,Kuragano M,Nozumi M,Igarashi M,Nakagawa H,Kotani S,Tokuraku K

    更新日期:2020-09-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhan Y,Yang C,Zhang Q,Yao L

    更新日期:2021-01-22 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tanaka Y,Yoshihara K,Tsuyuki M,Itaya-Hironaka A,Inada Y,Kamiya T

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  • Morphological and physicochemical changes in the myosin molecules induced by hydrostatic pressure.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamamoto K,Yoshida Y,Morita J,Yasui T

    更新日期:1994-07-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yagi T,Kirino J,Yamamoto S,Nozaki M

    更新日期:1985-10-01 00:00:00

  • G protein gamma subunits coimmunoprecipitated with antibodies against alpha subunits: identification of major isoforms in cultured cells by silver stain and immunoblotting with conventional transfer procedure.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ueda H,Morishita R,Katoh-Semba R,Kato K,Asano T

    更新日期:1998-11-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hatakeyama T,Suenaga T,Eto S,Niidome T,Aoyagi H

    更新日期:2004-01-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Masamoto K,Matsuura K,Itoh S,Nishimura M

    更新日期:1981-02-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Taguchi R,Ikezawa H

    更新日期:1977-11-01 00:00:00