Molecular and biochemical characterization of bifunctional pyruvate decarboxylases and pyruvate ferredoxin oxidoreductases from Thermotoga maritima and Thermotoga hypogea.

abstract：:We identified a protein with a molecular weight of about 44 kDa in an extract of rat regenerating liver. This protein was undetectable in normal, sham-operated, and completely regenerated liver. We also purified this 44-kDa protein from an extract of rat liver remnant after partial hepatectomy. The partial amino acid ...

journal_title：Journal of biochemistry

authors： Tanahashi T,Suzuki M,Itoh N,Mitsui Y

更新日期：1995-08-01 00:00:00

abstract：:Recently, we revealed that microtubule-associated protein (MAP) 4 isoforms, which differ in the number of repeat sequences, alter the microtubule surface properties, and we proposed a hypothesis stating that the change in the surface properties may regulate the movements of microtubule motors [Tokuraku et al. (2003) J...

journal_title：Journal of biochemistry

authors： Tokuraku K,Noguchi TQ,Nishie M,Matsushima K,Kotani S

更新日期：2007-04-01 00:00:00

abstract：:The time course of binding of N-ethylmaleimide (NEM) to the SR was measured at pH 7.5 in the presence or absence of ATP or ADP. The following results were obtained. 1. Both in the presence and absence of nucleotide, the ATPase [EC 3.6.1.3] activity decreased linearly with increase in the amount of NEM bound to the fra...

journal_title：Journal of biochemistry

authors： Yoshida H,Tonomura Y

更新日期：1976-03-01 00:00:00

abstract：:1. Chymotrypsin cleaved troponin-T of skeletal muscle into two subfragments, i.e., troponin-T1 and -T2, each of which could be isolated by the use of DEAE-Sephadex. Troponin-T1 was a single subfragment with a molecular weight of 26,000 (chicken) or 22,000 (rabbit) daltons. Troponin-T2 consisted of two subfragments wit...

journal_title：Journal of biochemistry

authors： Ohtsuki I

更新日期：1979-08-01 00:00:00

abstract：:A rat liver-specific antigen (RLSA) solubilized with the nonionic detergent non-anonyl-N-methylglucamide was purified through affinity column chromatography with a monoclonal antibody and by high-performance liquid chromatography with a hydroxylapatite column. The purified RLSA showed a single band on sodium dodecyl s...

journal_title：Journal of biochemistry

authors： Sato K,Ikeda T,Kawai F,Osada Y

更新日期：1987-10-01 00:00:00

abstract：:We have studied the protein composition of the pectoralis superficialis muscle of genetically dystrophic (New Hampshire line 413) and normal control (line 412) chickens by one- and two-dimensional gel electrophoresis. A protein, referred to hereafter as the 30 kDa abnormal protein, was specifically detected in the aff...

journal_title：Journal of biochemistry

authors： Kobayashi R,Toyoshima I,Masamune O,Tashima Y

更新日期：1990-01-01 00:00:00

abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...

journal_title：Journal of biochemistry

authors： Yokoyama A,Murata M,Oshima Y,Iwashita T,Yasumoto T

更新日期：1988-08-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:Transient and steady state kinetics were studied in the interactions of ATP with acto-H-meromyosin reconstituted from bovine arterial heavy-meromyosin (HMM) and rabbit skeletal muscle F-actin. The results showed that the rate of dissociation of the hybrid acto-HMM induced by ATP was slower than the rate of the fluores...

journal_title：Journal of biochemistry

authors： Takeuchi K,Tonomura Y

更新日期：1978-08-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:We found that transducing phages carrying the gal or bio regions of the Escherichia coli genome were formed during in vitro packaging of endogenous lambda DNA. Structural analysis of the transducing phage genomes indicated that they were formed by abnormal excision of lambda prophage. Formation of transducing phages w...

journal_title：Journal of biochemistry

authors： Tomono M,Shiozaki M,Ikeda H

更新日期：1989-03-01 00:00:00

abstract：:The primary structure of human IGF-I, except for the disulfide bond system, has been reported by Rinderknecht and Humbel. IGF-I afforded the corresponding characteristic peptide fragment on V8 protease digestion, which contained Cys6, Cys47, Cys48, and Cys52. Two possible fragments, Type I with Cys6-Cys47 and Cys48-Cy...

journal_title：Journal of biochemistry

authors： Iwai M,Kobayashi M,Tamura K,Ishii Y,Yamada H,Niwa M

更新日期：1989-12-01 00:00:00

abstract：:Calmodulin-dependent protein kinase IV (CaM-kinase IV) kinase was recently discovered in the rat brain by its activity to activate the inactive recombinant CaM-kinase IV expressed in Escherichia coli [Okuno, S. and Fujisawa, H. (1993) J. Biochem. 114, 167-170]. In the present study, CaM-kinase IV kinase was purified a...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：1994-10-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:Gastric cancer (GC) is one of the most common cancers in gastrointestinal malignant tumours. Long non-coding RNAs were widely reported to play a significant role in the regulation of occurrence or development of tumours. Bioinformatics analysis and a wide range of experiments were conducted to explore the expression s...

journal_title：Journal of biochemistry

authors： Xin H,Yan Z,Cao J

更新日期：2020-11-01 00:00:00

abstract：:Several trypsin inhibitors with different mobilities on polyacrylamide gel electrophoresis occur in the tubers of taro (Colocasia antiquorum), and they each have a dimeric molecular weight of 40,000. Of all the constituent subunits, molecular weight 20,000, of the taro trypsin inhibitor (TTI), three major subunit comp...

journal_title：Journal of biochemistry

authors： Ogata F,Makisumi S

更新日期：1985-02-01 00:00:00

abstract：:Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1...

journal_title：Journal of biochemistry

authors： Fukumori F,Takeuchi N,Hagiwara T,Ohbayashi H,Endo T,Kochibe N,Nagata Y,Kobata A

更新日期：1990-02-01 00:00:00

abstract：:The role of mannan chains in the formation and secretion of active acid phosphatase of yeast (Saccharomyces cerevisiae), a repressible cell surface mannoprotein, was studied in yeast protoplast systems by using tunicamycin at various temperatures. At 30 degrees C, tunicamycin-treated protoplasts did not produce active...

journal_title：Journal of biochemistry

authors： Mizunaga T,Izawa M,Ikeda K,Maruyama Y

更新日期：1988-02-01 00:00:00

abstract：:Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and spreading of hepatocytes on plates as monolayers. Another role is to maintain cell viability for over 2 days. For the first effect, serum could be replaced completel...

journal_title：Journal of biochemistry

authors： Horiuti Y,Nakamura T,Ichihara A

更新日期：1982-12-01 00:00:00

abstract：:The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the s...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：1984-06-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:Tetrahydrobiopterin (BH(4)) acts as a cofactor of the aromatic amino-acid hydroxylases, and its deficiency may result in hyperphenylalaninemia (HPA) and decreased production of the neurotransmitters. BH(4) is synthesized by sepiapterin reductase (SPR) from 6-pyruvoyl-tetrahydropterin (PPH(4)). A patient with SPR defic...

journal_title：Journal of biochemistry

authors： Hirakawa H,Sawada H,Yamahama Y,Takikawa S,Shintaku H,Hara A,Mase K,Kondo T,Iino T

更新日期：2009-07-01 00:00:00

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:We studied the roles of cofilin, an actin-binding phosphoprotein, in superoxide production of neutrophil-like HL-60 cells triggered by opsonized zymosan (OZ). OZ caused dephosphorylation of cofilin as well as a transient increase of F-actin. Both reactions were complete within 30 s. Okadaic acid (OA) magnified the OZ-...

journal_title：Journal of biochemistry

authors： Nagaishi K,Adachi R,Kawanishi T,Yamaguchi T,Kasahara T,Hayakawa T,Suzuki K

更新日期：1999-05-01 00:00:00

abstract：:We have cloned a chitinase cDNA (MpChi-1) from plantain (Musa paradisiacal L) using rapid amplification of cDNA ends (RACE) according to a sequence fragment which we had cloned using the suppression subtractive hybridization (SSH) technique. The MpChi-1 encodes a protein of 326 amino acids and belongs to acidic chitin...

journal_title：Journal of biochemistry

authors： Fan J,Wang H,Feng D,Liu B,Liu H,Wang J

更新日期：2007-11-01 00:00:00

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:A number of L-amino acids and derivatives were tested as substrates for the purified Pseudomonas L-phenylalanine oxidase. The reaction products of these amino acids were analyzed by high performance liquid chromatography and the kinetic properties of the reactions were partially characterized. In addition to L-phenyla...

journal_title：Journal of biochemistry

authors： Koyama H

更新日期：1984-08-01 00:00:00

abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

journal_title：Journal of biochemistry

authors： Maeng JH,Yoon JB

更新日期：1998-07-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00