Cyclic AMP antagonist Rp-cAMPS inhibits amylase exocytosis from saponin-permeabilized parotid acini.

abstract：:Soluble guanylate cyclase (sGC) consisting of two different subunits (alpha: Mr = 74,000, beta: Mr = 69,000) was purified more than 12,000-fold in terms of specific activity from the supernatant of bovine lung homogenates and characterized. The heme content determined with the pyridine hemochromogen method and Bradfor...

journal_title：Journal of biochemistry

authors： Tomita T,Tsuyama S,Imai Y,Kitagawa T

更新日期：1997-09-01 00:00:00

abstract：:In this work we have studied the equilibrium and kinetic stability of a hyperthermophilic protein, O(6)-methylguanine-DNA methyltransferase (Tk-MGMT), and its mesophilic counterpart AdaC, in various chemical solutions. In an unfolding experiment using guanidine hydrochloride (GdnHCl), the unfolding free-energy change ...

journal_title：Journal of biochemistry

authors： Nishikori S,Shiraki K,Okanojo M,Imanaka T,Takagi M

更新日期：2004-10-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:A simple and rapid purification method for human serum C-reactive protein (CRP) was developed. CRP was strongly adsorbed on a DEAE-cellulose column and was easily separated from other serum proteins. CRP was purified approximately 1,000-fold with a high yield (50%). The final preparation showed a single band as judged...

journal_title：Journal of biochemistry

authors： Tsujimoto M,Inoue K,Nojima S

更新日期：1983-11-01 00:00:00

abstract：:The betagamma subunits of G proteins were coimmunoprecipitated with antibodies against various alpha subunits, and analyzed by silver stain and immunoblotting with conventional transfer procedure and membrane-blocking buffer containing 2% BSA. Multiple isoforms of gamma were coimmunoprecipitated with no significant di...

journal_title：Journal of biochemistry

authors： Ueda H,Morishita R,Katoh-Semba R,Kato K,Asano T

更新日期：1998-11-01 00:00:00

abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

journal_title：Journal of biochemistry

authors： Kumagai JI,Ohta T,Imahori K

更新日期：1976-03-01 00:00:00

abstract：:The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG1 in the expression...

journal_title：Journal of biochemistry

authors： Inayoshi Y,Kaneoka H,Machida Y,Terajima M,Dohda T,Miyake K,Iijima S

更新日期：2005-10-01 00:00:00

abstract：:To study how collagen synthesis is regulated in developing chick embryonic skin, hydroxyproline synthesis, incorporation of proline, and translational activity and content of collagen mRNA in 12-, 15-, and 18-day skins were determined and compared with each other. Hydroxyproline synthesis in the 18-day skins was marke...

journal_title：Journal of biochemistry

authors： Ohyama K,Tokimitsu I,Tajima S

更新日期：1991-08-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:Mitochondrial DNA of an erythromycin-resistant petite mutant of yeast, E734, showed physical maps of inversion, which occurred between two cross-over sites in the fragments Hae A and Hae B. The pair of cross-over sites was inferred to be accommodated within a repeat unit of E734 mtDNA during petite mutation by joining...

journal_title：Journal of biochemistry

authors： Wakabayashi K,Furutani Y

更新日期：1984-11-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00

abstract：:We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

journal_title：Journal of biochemistry

authors： Yamamoto K

更新日期：1993-12-01 00:00:00

abstract：:Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was r...

journal_title：Journal of biochemistry

authors： Degawa M,Namiki M,Yoshimoto N,Makino M,Iwamoto M,Nemoto K,Hashimoto Y

更新日期：2003-06-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:Thermal denaturation of cytochromes c of horse, cow, and Candida krusei in aqueous guanidine hydrochloride in the neutral pH region was studied by means of absorption and optical rotation measurements. The values of standard free energy change upon denaturation were estimated over the temperature range from 3 to 51 de...

journal_title：Journal of biochemistry

authors： Kawaguchi H,Noda H

更新日期：1977-05-01 00:00:00

abstract：:During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

journal_title：Journal of biochemistry

authors： Iwamuro S,Saeki M,Kato S

更新日期：1999-07-01 00:00:00

abstract：:A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase ...

journal_title：Journal of biochemistry

authors： Takamura H,Kito M

更新日期：1991-03-01 00:00:00

abstract：:It was found that the absorption spectrum of beta-naphthyl triphosphate is different from that of beta-naphthyl diphosphate in the range 290-335 nm. Thus, beta-naphthyl triphosphate hydrolysis by heavy meromyosin can be recorded continuously as a function of time by means of a spectrophotometer. By analyzing the time ...

journal_title：Journal of biochemistry

authors： Kuwajima T,Kagawa H,Asai H

更新日期：1975-11-01 00:00:00

abstract：:Detergent treatments were examined for their efficacy in purifying trimethylamine-N-oxide (TMAO) demethylase activity from fish muscle microsomes. Tritons X-100 and X-45, deoxycholate, Brijs, Tweens 20, 65, and 80, and SDS were generally ineffective in solubilizing demethylase activity from this membrane fraction, at ...

journal_title：Journal of biochemistry

authors： Parkin KL,Hultin HO

更新日期：1986-07-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:C3-translational product, which was synthesized with rabbit liver mRNA in a reticulocyte lysate protein-synthesizing system, did not react with [14C]methylamine, indicating the lack of an internal thioester bond. Instead, the C3-translational product reacted with iodo[1-14C]acetamide, as determined by gel electrophore...

journal_title：Journal of biochemistry

authors： Iijima M,Tobe T,Sakamoto T,Tomita M

更新日期：1984-11-01 00:00:00

abstract：:Frontal gel chromatography is a convenient and accurate method to obtain the free ligand concentration of a protein-ligand mixture. Because a large amount of sample (more than 6 ml) is required for the method, it has been rarely used for binding experiments. We have developed a system to carry out frontal gel chromato...

journal_title：Journal of biochemistry

authors： Honjo M,Ishida T,Horiike K

更新日期：1997-08-01 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:We prepared a streptavidin-based neoglycoprotein which carries more than 140 GT1b oligosaccharides. GT1b oligosaccharides were covalently coupled to streptavidin by reductive amination, yielding a monomer form of streptavidin carrying 13 oligosaccharides. The monomer form of glycosylated streptavidin was polymerized w...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki M,Crocker PR,Suzuki A

更新日期：1998-03-01 00:00:00

abstract：:Eph/ephrin signalling plays essential roles in various tissue developments, such as axon guidance, angiogenesis and tissue separation. Interaction between Ephs and ephrins upon cell-cell contact results in forward (towards Eph-expressing cells) and reverse (towards ephrin-expressing cells) signalling. Although the mol...

journal_title：Journal of biochemistry

authors： Takeuchi S,Katoh H,Negishi M

更新日期：2015-09-01 00:00:00

abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

journal_title：Journal of biochemistry

authors： Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

更新日期：1980-03-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, applying the computer searching method to primary ...

journal_title：Journal of biochemistry

authors： Iida Y

更新日期：1985-04-01 00:00:00

abstract：:Polynucleotide phosphorylase from Thermus thermophilus catalyzed the addition of short guanylyl blocks from GDP to the 3'-hydroxyl termini of oligonucleotide primers at low temperature in a simple reaction mixture. Polyguanylic acid formation was inhibited at 37 degrees C, but the addition of one or two guanylyl resid...

journal_title：Journal of biochemistry

authors： Kikuchi Y,Hirai K,Sakaguchi K

更新日期：1979-11-01 00:00:00