Purification and characterization of human serum C-reactive protein.

abstract：:Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...

journal_title：Journal of biochemistry

authors： Okubo T,Yanai N,Watanabe S,Arai Ki,Obinata M

更新日期：2000-04-01 00:00:00

abstract：:The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

journal_title：Journal of biochemistry

authors： Ohta M,Matsumoto H,Ishii K,Ueno Y

更新日期：1978-09-01 00:00:00

abstract：:TreP [trehalose-permease (phosphotransferase system (PTS) trehalose-specific enzyme IIBC component)] is one of the target proteins of tmRNA-mediated trans-translation in Bacillus subtilis [Fujihara et al. (2002) Detection of tmRNA-mediated trans-translation products in Bacillus subtilis. Genes Cells, 7, 343-350]. The ...

journal_title：Journal of biochemistry

authors： Ujiie H,Matsutani T,Tomatsu H,Fujihara A,Ushida C,Miwa Y,Fujita Y,Himeno H,Muto A

更新日期：2009-01-01 00:00:00

abstract：:The gene coding for a Trichosanthes trypsin inhibitor analog (Ala-6-TTI) in which methionine at position 6 was replaced by alanine was synthesized chemically. The synthetic gene was cloned into plasmid pWR590-1 and expressed in Escherichia coli as a fusion protein composed of beta-galactosidase fragment of 590 amino a...

journal_title：Journal of biochemistry

authors： Chen XM,Qian YW,Chi CW,Gan KD,Zhang MF,Chen CQ

更新日期：1992-07-01 00:00:00

abstract：:Plant cell-wall arabinoxylans have a complex structure that requires the action of a pool of debranching (arabinofuranosidases) and depolymerizing enzymes (endo-xylanase). Two Aspergillus nidulans strains over-secreting endo-xylanase and arabinofuranosidase were inoculated in defined 2% maltose-minimum medium resultin...

journal_title：Journal of biochemistry

authors： Damásio AR,Pessela BC,da Silva TM,Guimarães LH,Jorge JA,Guisán JM,Polizeli Mde L

更新日期：2013-09-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00

abstract：:Human matrix metalloproteinase 7 (MMP-7) activity exhibits broad bell-shaped pH profile with the acidic and alkaline pK(a) (pK(e1) and pK(e2)) values of about 4 and 10. The ionizable group for pK(e2) was assigned to Lys or Arg by thermodynamic analysis; however, no such residues are present in the active site. Hence, ...

journal_title：Journal of biochemistry

authors： Morishima A,Yasukawa K,Inouye K

更新日期：2012-05-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:The 20,000 dalton light chain (L2) was isolated from rabbit and chicken striated muscle myosins, and the Ca2+-induced conformational changes of these proteins were investigated. 1) The reaction of thiol groups of L2 with dithiobisnitrobenzoic acid (DTNB), 2) measurements of the UV difference absorption spectrum, 3) me...

journal_title：Journal of biochemistry

authors： Minowa O,Matsuda S,Yagi K

更新日期：1983-07-01 00:00:00

abstract：:The intracellular distribution of 10Sa RNA in Escherichia coli was investigated in cell extracts. Northern hybridization revealed that a large fraction of 10Sa RNA cosediments with 70S ribosomes. When 70S ribosomes were dissociated into 50S and 30S subunits in the presence of low levels of Mg2+ ions, almost all of the...

journal_title：Journal of biochemistry

authors： Komine Y,Kitabatake M,Inokuchi H

更新日期：1996-03-01 00:00:00

abstract：:The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the...

journal_title：Journal of biochemistry

authors： Yamamoto S,Okujo N,Yoshida T,Matsuura S,Shinoda S

更新日期：1994-05-01 00:00:00

abstract：:Chicken brain enolase was found to show multiple forms (I, II and III) separable by DEAE-cellulose column chromatography, whereas enolase from chicken skeletal muscle showed a single form. Brain enolase I, enolase III and muscle enolase were purified to electrophoretic homogeneity. These three isozymes were dimeric en...

journal_title：Journal of biochemistry

authors： Tanaka M,Sugisaki K,Nakashima K

更新日期：1985-12-01 00:00:00

abstract：:The binding of ADP to subfragment-1 was investigated by the gel filtration method. The amount of bound ADP was determined as a function of free ADP concentration. Linear Scatchard plots were obtained. The maximum binding number, 0.55 mole of ADP per 10(5) g of protein, and the dissociation constant, 1.6 x 10(-6) M, we...

journal_title：Journal of biochemistry

authors： Yoshida M,Morita F

更新日期：1975-05-01 00:00:00

abstract：:By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

journal_title：Journal of biochemistry

authors： Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

更新日期：1982-06-01 00:00:00

abstract：:A completely sodium dodecyl sulfate-insoluble protein was obtained from plasmodia of Physarum polycephalum. The amino acid composition of this protein was very similar to that of connectin, an elastic protein of muscle. When the protein suspension was treated with 6 M guanidine-HCl in the presence of 0.1 M 2-mercaptoe...

journal_title：Journal of biochemistry

authors： Ozaki K,Maruyama K

更新日期：1980-09-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

journal_title：Journal of biochemistry

authors： Andoh R,Yamamoto T

更新日期：1985-03-01 00:00:00

abstract：:Connectin (titin) links the Z line to the myosin filament in sarcomeres of vertebrate skeletal muscle. An 800 kDa fragment of alpha-connectin runs from the Z line up to the N2 line region in the I band, and the following beta-connectin portion runs up to the edge of the M line on the myosin filament in chicken breast ...

journal_title：Journal of biochemistry

authors： Kawamura Y,Kume H,Itoh Y,Ohtsuka S,Kimura S,Maruyama K

更新日期：1995-01-01 00:00:00

abstract：:The light protein components of silk fibroin were analyzed by Western blotting using polyclonal antibodies against two synthetic peptides that are segment of the light (L-) chain [Yamaguchi et al. (1989) J. Mol. Biol. 210, 127-139] and P25 [Chevillard et al. (1986) Sericologia 26, 435-449], respectively, and against t...

journal_title：Journal of biochemistry

authors： Tanaka K,Mori K,Mizuno S

更新日期：1993-07-01 00:00:00

abstract：:A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state vi...

journal_title：Journal of biochemistry

authors： Nishida E,Kuwaki T,Maekawa S,Sakai H

更新日期：1981-05-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:A form of UDP-glucuronyltransferase has been purified from liver microsomes of 3-methylcholanthrene-treated rats by a simple and rapid method involving chromatography on DEAE-Toyopearl and UDP-hexanolamine Sepharose columns. The purified preparation gave a single protein band (Mr 54,000) on sodium dodecyl sulfate-poly...

journal_title：Journal of biochemistry

authors： Yokota H,Yuasa A,Sato R

更新日期：1988-10-01 00:00:00

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:As a new strategy for drug discovery and development, I focus on drug re-profiling as a way to identify new treatments for diseases. In this strategy, the actions of existing medicines, whose safety and pharmacokinetic effects in humans have already been confirmed clinically and approved for use, are examined comprehe...

journal_title：Journal of biochemistry

authors： Mizushima T

更新日期：2011-05-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:Many membrane proteins such as ion channels are oligomers, but the determinants of the degree of oligomerization are not fully understood. Mechanosensitive channel with large conductance (MscL), which is ubiquitous in bacteria, is a homopentamer with two transmembrane helices and a cytoplasmic helix in each subunit. T...

journal_title：Journal of biochemistry

authors： Ando C,Liu N,Yoshimura K

更新日期：2015-08-01 00:00:00

abstract：:During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

journal_title：Journal of biochemistry

authors： Iwamuro S,Saeki M,Kato S

更新日期：1999-07-01 00:00:00

abstract：:Gp91(phox) is a key component of the phagocyte NADPH oxidase. Mutations of its promoter found in patients with chronic granulomatous disease cause deficient binding of PU.1 and HAF-1. Because the two factors bind to the same site (Pu box) of the promoter, we attempted to clarify their relative in vivo contributions to...

journal_title：Journal of biochemistry

authors： Islam MR,Fan C,Fujii Y,Hao LJ,Suzuki S,Kumatori A,Yang D,Rusvai E,Suzuki N,Kikuchi H,Nakamura M

更新日期：2002-04-01 00:00:00

abstract：:We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 m...

journal_title：Journal of biochemistry

authors： Nagino K,Nomura O,Takii Y,Myomoto A,Ichikawa M,Nakamura F,Higasa M,Akiyama H,Nobumasa H,Shiojima S,Tsujimoto G

更新日期：2006-04-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00