Abstract:
:The 20,000 dalton light chain (L2) was isolated from rabbit and chicken striated muscle myosins, and the Ca2+-induced conformational changes of these proteins were investigated. 1) The reaction of thiol groups of L2 with dithiobisnitrobenzoic acid (DTNB), 2) measurements of the UV difference absorption spectrum, 3) measurements of Stokes radius (Rs) by gel filtration and 4) measurements of the ESR spectrum of L2 whose cysteine or tyrosine residues were spin-labeled were used for the structural studies. The effect of Ca2+ on phosphorylated L2 was also investigated. The long axis of chicken L2 was calculated as 136A from the Stokes radius, suggesting that the L2 is an asymmetrical molecule. After the addition of Ca2+ the long axis was reduced to 104 A. The same effect of Ca2+ has been reported with rabbit L2 (Alexis, N.M. & Gratzer, W.B. (1978) Biochemistry 17, 2319-2325). Besides this large shape change, the addition of Ca2+ to L2 induced environmental changes around tyrosine residues and also changes in the reactivity of cysteine residues with DTNB. Ca2+ is supposed to be bound to the N-terminal region of the molecule, while the tyrosine and cysteine residues are located at the C-terminal region, which is probably sterically remote from the N-terminal region. The reason for the remote effect of Ca2+ may be related to the structural rigidity of the L2 molecule. The functions of two properties of L2, Ca2+-binding and phosphorylation, are discussed in relation to muscle contraction.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Minowa O,Matsuda S,Yagi Kdoi
10.1093/oxfordjournals.jbchem.a134337subject
Has Abstractpub_date
1983-07-01 00:00:00pages
25-35issue
1eissn
0021-924Xissn
1756-2651journal_volume
94pub_type
杂志文章abstract::A novel type of sulfotransferase was purified from Klebsiella K-36, an intestinal bacterium of rat. The enzyme (M(r) 160,000) is composed of two subunits (M(r) 73,000) with pI and optimal pH values of 5.3 and 10-10.5, respectively. The apparent Km for PNS (p-nitrophenyl sulfate) using phenol as an acceptor and that fo...
journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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更新日期:1983-01-01 00:00:00
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更新日期:1987-12-01 00:00:00
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doi:10.1093/oxfordjournals.jbchem.a134216
更新日期:1983-02-01 00:00:00
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doi:10.1093/oxfordjournals.jbchem.a130759
更新日期:1975-03-01 00:00:00
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更新日期:1986-03-01 00:00:00
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更新日期:1993-11-01 00:00:00
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更新日期:1985-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123573
更新日期:1991-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134609
更新日期:1984-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133858
更新日期:1982-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123823
更新日期:1992-06-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1994-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
abstract::A contractile protein closely resembling natural actomyosin (myosin B) of rabbit skeletal muscle was extracted from plasmodia of the slime mold, Physarum polycephalum, by protecting the SH-groups with beta-mercaptoethanol or dithiothreitol. Superprecipitation of the protein induced by Mg2+-ATP at low ionic strength wa...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00