Enzymatic deacetylation of N-acetylglucosamine residues in cell wall peptidoglycan.

Abstract:

:An enzyme which catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine residues in cell wall peptidoglycan was found in the supernatant and 20,000 X g pellet fractions of Bacillus cereus. Autolysis of the latter fraction resulted in solubilization and activation of the deacetylase. Among various bacteria, strains of B. cereus which contain high proportions of N-unsubstituted glucosamine residues in their cell wall peptidoglycan components are particularly rich in the deacetylase. The peptidoglycan deacetylase is distinguishable from N-acetylglucosamine-6-phosphate deacetylase [EC 3.5.1.25] on the basis of their cellular distribution and chromatographic behavior. The rate of reaction of the deacetylase with (N-acetylglucosaminyl-N-acetylmuramic acid)3 [abbreviated as (GlcNAc-MurNAc)3] is less than 1/100 of that with peptidoglycan, while the enzyme is inactive towards (GlcNAc-MurNAc)2, GlcNAc-MurNAc, and monomeric N-acetylglucosamine derivatives. The enzyme also deacetylates partially O-hydroxyethylated chitin. The concentrations of peptidoglycan and partially O-hydroxyethylated chitin required for half-maximum activities were found to be 0.29 and 6.9 mg per ml (or 0.17 and 20 mM with respect to N-acetylglucosamine residues), respectively. The occurrence of this enzyme accounts for the formation of cell wall peptidoglycan N-unsubstituted at the glucosamine residues.

journal_name

J Biochem

journal_title

Journal of biochemistry

authors

Araki Y,Oba S,Araki S,Ito E

doi

10.1093/oxfordjournals.jbchem.a132994

subject

Has Abstract

pub_date

1980-08-01 00:00:00

pages

469-79

issue

2

eissn

0021-924X

issn

1756-2651

journal_volume

88

pub_type

杂志文章
  • Determination of the protein components of native thin filaments isolated from natural actomyosin: nebulin and alpha-actinin are associated with actin filaments.

    abstract::A new method was developed for extracting natural actomyosin with full length connectin (titin) and nebulin from rabbit skeletal muscle. To determine the protein components of native thin filaments, thin filaments were isolated from natural actomyosin by sedimentation in a H2O/D2O/sucrose density gradient. Both alpha-...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124924

    authors: Meng Y,Yasunaga T,Wakabayashi T

    更新日期:1995-08-01 00:00:00

  • Alpha-casein-binding proteins of guinea pig macrophage membranes and their possible roles in chemotaxis.

    abstract::The existence of specific binding proteins for alpha-casein, a well-known potent chemoattractant, on guinea pig peritoneal macrophages was demonstrated. Binding of 3H-alpha-casein to macrophages was found to be specific and reversible. Its association constant and the number of binding sites were 5.0 X 10(-6) M and 7....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a132883

    authors: Katagiri T,Adachi I,Terao T,Osawa T

    更新日期:1980-05-01 00:00:00

  • Recognition of allylic substrates in Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase: analysis using mutated enzymes and artificial allylic substrates.

    abstract::We examined the substrate specificity of two mutated geranylgeranyl diphosphate synthases, I-9 and I-11, with respect to several artificial substrates. These mutated enzymes have replacements in the amino acid sequences from positions 170 to 173, which are thought to be a part of the putative substrate binding region....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a022040

    authors: Ohnuma S,Hemmi H,Koyama T,Ogura K,Nishino T

    更新日期:1998-06-01 00:00:00

  • Further study on association of 5SrRNA-L5 protein complex and methionyl-tRNA to methionyl-tRNA synthetase in the macromolecular aminoacyl-tRNA synthetase complex.

    abstract::To obtain direct evidence for the attachment of 5SrRNA-ribosomal L5 protein particles (5SRNP) and methionine-tRNA (tRNA(met)) to methionyl-tRNA synthetase (MetRS) in the macromolecular aminoacyl-tRNA synthetase (ARS) complex of rat liver, a MetRS-5SRNP-tRNA(met) complex was dissociated from the macromolecular ARS comp...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021500

    authors: Ogata K,Ohno R,Morioka S,Terao K

    更新日期:1996-11-01 00:00:00

  • Autotaxin--an LPA producing enzyme with diverse functions.

    abstract::Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审

    doi:10.1093/jb/mvq052

    authors: Nakanaga K,Hama K,Aoki J

    更新日期:2010-07-01 00:00:00

  • Importance of the ionic nature of ionic liquids in affecting enzyme performance.

    abstract::The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF(6)], [BMIm][BF(4)]), and [BMIm][MeSO(4)], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO(4), KPF(6), and NaBF(4)) on the enzyme performance wa...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvn173

    authors: Yang Z,Yue YJ,Huang WC,Zhuang XM,Chen ZT,Xing M

    更新日期:2009-03-01 00:00:00

  • Functionally active thrombomodulin is present in human platelets.

    abstract::We found functionally active thrombomodulin in human platelets (60 +/- 18 molecules per platelet). Protein C appeared not to be activated by thrombin with gel-filtered platelets. However, the activation of protein C by thrombin was accelerated by thrombin-stimulated and washed platelets. This cofactor activity of the ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a122523

    authors: Suzuki K,Nishioka J,Hayashi T,Kosaka Y

    更新日期:1988-10-01 00:00:00

  • Co-immobilization of fungal endo-xylanase and α-L-arabinofuranosidase in glyoxyl agarose for improved hydrolysis of arabinoxylan.

    abstract::Plant cell-wall arabinoxylans have a complex structure that requires the action of a pool of debranching (arabinofuranosidases) and depolymerizing enzymes (endo-xylanase). Two Aspergillus nidulans strains over-secreting endo-xylanase and arabinofuranosidase were inoculated in defined 2% maltose-minimum medium resultin...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvt053

    authors: Damásio AR,Pessela BC,da Silva TM,Guimarães LH,Jorge JA,Guisán JM,Polizeli Mde L

    更新日期:2013-09-01 00:00:00

  • Identification of amino acid residues responsible for the changes of absorption and fluorescence spectra on the binding of subtilisin BPN' and Streptomyces subtilisin inhibitor.

    abstract::An ultraviolet absorption difference spectrum characteristic of the ionization change of a tyrosyl residue was observed on the binding of subtilisin BPN' with Streptomyces subtilisin inhibitor (SSI) at alkaline pH. This difference spectrum was considered to be induced by a pKa shift (from 9.7 to > or = 11.5) of a tyro...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124275

    authors: Masuda-Momma K,Shimakawa T,Inouye K,Hiromi K,Kojima S,Kumagai I,Miura K,Tonomura B

    更新日期:1993-12-01 00:00:00

  • Nature of the binding site around and reactivity of histidine-15 in lysozyme.

    abstract::Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a134632

    authors: Yamada H,Uozumi F,Ishikawa A,Imoto T

    更新日期:1984-02-01 00:00:00

  • A screening system for artificial small RNAs that inhibit the growth of Escherichia coli.

    abstract::We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvr055

    authors: Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

    更新日期:2011-09-01 00:00:00

  • Interaction of mitochondrial aspartate aminotransferase with negatively charged lecithin liposomes.

    abstract::Several kinds of hydrophilic proteins were examined to determine their interaction with artificial liposomes. Mitochondrial aspartate aminotransferase (m-GOT) [EC 2.6.1.1], as well as cytochrome c, was found to interact strongly with negatively charged liposomes. In each case, an appreciable amount of the protein boun...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:

    authors: Furuya E,Yoshida Y,Tagawa K

    更新日期:1979-05-01 00:00:00

  • Localization of the phosphatidylserine-binding site of glyceraldehyde-3-phosphate dehydrogenase responsible for membrane fusion.

    abstract::In this study, we demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phosphatidylserine (PS)-binding protein and localized the putative PS-binding site involved in the membrane fusion induced by this enzyme. In an attempt to identify the PS-binding proteins, we raised polyclonal antibodies against...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021886

    authors: Kaneda M,Takeuchi K,Inoue K,Umeda M

    更新日期:1997-12-01 00:00:00

  • A mechanism of respiratory control: studies with proteoliposomes containing cytochrome oxidase and bacteriorhodopsin.

    abstract::Both beef heart cytochrome oxidase and bacteriorhodopsin of Halobacterium halobium were reconstituted into liposomes by the sonication-cholate dialysis method. The proteoliposomes showed the respiratory control ratio of 4.2, and steady-state illumination of the vesicles lead to the 2.7-fold stimulation of the oxidase ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a122033

    authors: Miki T,Orii Y,Mukohata Y

    更新日期:1987-07-01 00:00:00

  • Ca2+- and S1-induced conformational changes of reconstituted skeletal muscle thin filaments observed by fluorescence energy transfer spectroscopy: structural evidence for three States of thin filament.

    abstract::Rabbit skeletal muscle alpha-tropomyosin (Tm) and the deletion mutant (D234Tm) in which internal actin-binding pseudo-repeats 2, 3, and 4 are missing [Landis et al. (1997) J. Biol. Chem. 272, 14051-14056] were used to investigate the interaction between actin and tropomyosin or actin and troponin (Tn) by means of fluo...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a003116

    authors: Hai H,Sano K,Maeda K,Maéda Y,Miki M

    更新日期:2002-03-01 00:00:00

  • Purification and properties of xanthine dehydrogenase from Streptomyces cyanogenus.

    abstract::Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:

    authors: Ohe T,Watanabe Y

    更新日期:1979-07-01 00:00:00

  • Altered activity of bacteriorhodopsin in high concentrations of guanidine hydrochloride.

    abstract::Bacteriorhodopsin from Halobacterium halobium was stable and active in high concentrations of guanidine hydrochloride. However the decay of the intermediate with absorption maximum at 407 nm and the uptake of protons from the medium were remarkably delayed. At alkaline pH values, all the molecules of bacteriohodopsin ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a132769

    authors: Yoshida M,Ohno K,Takeuchi Y

    更新日期:1980-02-01 00:00:00

  • Different buffer effects in selecting HM-1 killer toxin single-chain fragment variable anti-idiotypic antibodies.

    abstract::We reported previously competitive panning elution with PBS (pH 7.0) that contains HM-1 killer toxin (HM-1) and Candida albicans membrane fraction (CaMF) to release phages bound with CaMF as an antigen. Here, as an alternative strategy, we isolated high-binding affinity recombinant single-chain fragment variables (scF...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvq006

    authors: Krishnaswamy S,Kabir ME,Miyamoto M,Furuichi Y,Komiyama T

    更新日期:2010-05-01 00:00:00

  • Uncoupling of ATP splitting from Ca(2+)-transport in Ca(2+)-transporting ATPase of the sarcoplasmic reticulum as a result of modification by N-(3-pyrene)maleimide: activation of a channel with a specificity for alkaline earth metal ions.

    abstract::The sarcoplasmic reticulum (SR) membranes of rabbit skeletal muscle were allowed to react with N-(3-pyrene)maleimide (PMI) at pH 7 at 30 degrees C. The Ca(2+)-transporting activity of the SR membranes was reduced to 20% when PMI was bound to the extent of 1 mol/mol of Ca(2+)-transporting ATPase. The ATPase and the E-P...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124155

    authors: Suzuki T,Kawakita M

    更新日期:1993-08-01 00:00:00

  • Effects of random mutagenesis in a putative substrate-binding domain of geranylgeranyl diphosphate synthase upon intermediate formation and substrate specificity.

    abstract::Archaeal geranylgeranyl diphosphate (GGPP) synthase catalyzes the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce GGPP with significant amounts of intermediates. To obtain information about the amino acids involved in the condensation and the release of intermediates, we ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021642

    authors: Ohnuma S,Hemmi H,Ohto C,Nakane H,Nishino T

    更新日期:1997-04-01 00:00:00

  • Molecular characterization of a novel beta1,3-galactosyltransferase for capsular polysaccharide synthesis by Streptococcus agalactiae type Ib.

    abstract::A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a003086

    authors: Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

    更新日期:2002-02-01 00:00:00

  • Effects of modification of 4-thiouridine in E. coli tRNA(fMet) on its methyl acceptor activity by thermostable Gm-methylases.

    abstract::tRNA(guanosine-2'-)-methyltransferases (Gm-methylases) isolated from extreme thermophiles, Thermus thermophilus strains HB 27 and HB 8, methylate the 2'-OH of the G18 ribose of the GG sequence in the D loop of tRNA, by recognizing the D "loop-stem" structure as a minimal requirement. To examine the role of the consens...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a122933

    authors: Hori H,Saneyoshi M,Kumagai I,Miura K,Watanabe K

    更新日期:1989-11-01 00:00:00

  • Molecular cloning and characterization of two zebrafish alpha(1,3)fucosyltransferase genes developmentally regulated in embryogenesis.

    abstract::Some alpha(1,3)fucosylated oligosaccharides serve as counter receptors to lectin-like adhesion proteins or are expressed with temporal precision during embryogenesis, and alpha(1, 3)fucosyltransferase is a key enzyme in the production of these oligosaccharides. Two alpha(1,3)-fucosyltransferase genes, designated zFT1 ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a022357

    authors: Kageyama N,Natsuka S,Hase S

    更新日期:1999-04-01 00:00:00

  • Electrostatic recognition of matrix targeting signal by mitochondrial processing peptidase.

    abstract::Mitochondrial processing peptidase (MPP), a metalloendopeptidase consisting of alpha and beta subunits, specifically recognizes a large variety of mitochondrial precursor proteins and cleaves off the N-terminal basic matrix targeting signals (MTS). Basic residues in MTS and acidic sites in MPP are required for effecti...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a002827

    authors: Kitada S,Ito A

    更新日期:2001-01-01 00:00:00

  • Fructose 2,6-bisphosphate in human erythrocytes.

    abstract::The fructose 2,6-bisphosphate concentrations in unwashed, washed, and leukocyte-free erythrocytes were compared. The concentration in washed red cells was 31 +/- 15 pmol per ml of cells (mean +/- S.D., n = 6). The concentration in unwashed erythrocytes was at least twofold higher, but the value in washed red cells was...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a122384

    authors: Moriyasu M,Yamamoto M,Hamasaki N

    更新日期:1988-06-01 00:00:00

  • Different consequences of reactions with hydrogen peroxide and t-butyl hydroperoxide in the hyperoxidative inactivation of rat peroxiredoxin-4.

    abstract::Eukaryotic typical 2-Cys type peroxiredoxin (Prx) is inactivated by hyperoxidation of the peroxidatic cysteine to a sulphinic acid in a catalytic cycle-dependent manner. This inactivation process has been well documented for cytosolic isoforms of Prx. However, such a hyperoxidative inactivation has not fully been inve...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvq156

    authors: Ikeda Y,Nakano M,Ihara H,Ito R,Taniguchi N,Fujii J

    更新日期:2011-04-01 00:00:00

  • Mutation in the a-subunit of F(1)F(O)-ATPase causes an increased motility phenotype through the sodium-driven flagella of Vibrio.

    abstract::Bacterial flagellar motors exploit the electrochemical potential gradient of a coupling ion as energy source and are composed of stator and rotor proteins. Vibrio alginolyticus has a Na(+)-driven motor and its stator is composed of PomA and PomB. Recently, we isolated increased motility strains (sp1-sp4) from the PomA...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvt042

    authors: Terashima H,Terauchi T,Ihara K,Nishioka N,Kojima S,Homma M

    更新日期:2013-08-01 00:00:00

  • delta-Aminolevulinate synthetases in the liver cytosol fraction and mitochondria of mice treated with allylisopropylacetamide and 3,5-dicarbethoxyl-1,4-dihydrocollidine.

    abstract::Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecul...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a131365

    authors: Igarashi J,Hayashi N,Kikuchi G

    更新日期:1976-11-01 00:00:00

  • Proteoglycan synthesis by corneal explants from developing embryonic chicken.

    abstract::Proteoglycan biosynthesis in developing chicken corneas was studied. Corneas free of scleral rims were prepared from 5- to 18-day-old chick embryos and labeled in vitro with [3H]glucosamine and [35S]sulfate. Then, the labeled medium and corneal proteoglycans were analyzed. Day 5 corneas synthesized mainly chondroitin ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124767

    authors: Nakazawa K,Suzuki S,Wada K,Nakazawa K

    更新日期:1995-04-01 00:00:00

  • Thermodynamics of association of egg yolk riboflavin binding protein with 8-substituted riboflavins. Comparison with the egg white protein.

    abstract::The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133823

    authors: Matsui K,Sugimoto K,Kasai S

    更新日期:1982-04-01 00:00:00